Abstract：
:Prior studies have shown that filarial nematodes can effectively metabolize hydrogen peroxide in excess of that generated by activated host cells. However, the mechanisms of H2O2 removal by the filarial parasites are unclear. Herein we report the results of studies carried out on the biochemical activity and on immunolocalization of a recombinant peroxiredoxin (Prx) enzyme from the dog filarial parasite Dirofilaria immitis. A full-length cDNA encoding a 1-Cys Prx enzyme from the dog heartworm D. immitis was expressed in Escherichia coli as a recombinant polyhistidine fusion protein (rDiPrx-1). rDiPrx-1 was capable of reducing H2O2 in the presence of dithiothreitol. The apparent kinetic constants determined for DiPrx-1 using H2O2 as a substrate were a Michaelis constant (Km) of 16.28 mM and a maximal velocity (Vmax) of 16 micromol/min(-1). Consistent with the enzyme activity, D. immitis adult worms could detoxify exogenously added H2O2 in vitro. Antibodies to rDiPrx-1 identified a 27-kDa native antigen in parasite extracts and larval and adult excretory-secretory products. The antibodies were used to localize the native antigen to the lateral hypodermal chords of both male and female worms by immunohistochemistry. In addition, labeling was seen in the afibrillar muscle cells in male worms and in some areas of the uterine wall in female worms. Thus, DiPrx-1 is the first parasite Prx to be shown to detoxify exogenously added H2O2 in an in vitro system.
journal_name
Parasitol Resjournal_title
Parasitology researchauthors
Chandrashekar R,Tsuji N,Morales TH,Carmody AB,Ozols VO,Welton J,Tang Ldoi
10.1007/s004360050032subject
Has Abstractpub_date
2000-03-01 00:00:00pages
200-6issue
3eissn
0932-0113issn
1432-1955journal_volume
86pub_type
杂志文章abstract：:Studies were conducted in Mwea Rice Scheme, Kenya during the period April 2005 and January 2007 to determine the host-feeding pattern of culicine mosquitoes. Mosquitoes were collected indoors and outdoors and tested for human, bovine, goat, and donkey blood meals by an Enzyme-Linked Immunosorbent Assay. A total of 1,7...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-008-0914-7
更新日期：2008-05-01 00:00:00
abstract：:Praziquantel, due to high efficacy, excellent tolerability, few and transient side effects, simple administration, and competitive cost, is virtually the only drug of choice for treatment of human schistosomiasis. Treatment of schistosomiasis has shown great advances with the introduction of the drug into the therapeu...
journal_title：Parasitology research
pub_type： 杂志文章,评审
doi：10.1007/s00436-011-2670-3
更新日期：2011-12-01 00:00:00
abstract：:Larvae of the eye fluke, Diplostomum, emerge from snails and infect fish by penetrating skin or gills, then move to the lens where they may impair the vision of the fish. For the fluke to reproduce, a bird must eat the infected fish, and it has been suggested that they therefore actively manipulate the fish's behaviou...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-017-5439-5
更新日期：2017-06-01 00:00:00
abstract：:To contribute to the knowledge of the role of small mammals in the transmission cycle of tegumentary leishmaniasis caused by Leishmania braziliensis, we studied the small mammal community and its temporal and spatial association with phlebotominae, as well as small mammal infection by Leishmania spp. by PCR-RFLP analy...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-017-5714-5
更新日期：2018-02-01 00:00:00
abstract：:A polymerase chain reaction (PCR) assay followed by partial sequencing of the 16S ribosomal RNA gene was performed for the presence of Ehrlichia and/or Anaplasma. A total of 242 ixodid ticks were collected from domestic ruminants and their shelters, as well as humans, and their individual salivary glands were dissecte...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-009-1377-1
更新日期：2009-04-01 00:00:00
abstract：:Intestines of California sea lion (Zalophus californianus) pups (n= 204), born in 2002 on San Miguel Island, California, were examined for hookworms (Uncinaria spp.) as part of a seasonal mortality study from June through December 2002 and January 2003. The investigation was planned to coincide with most of the previo...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-005-1335-5
更新日期：2005-05-01 00:00:00
abstract：:Hassalstrongylus epsilon is a small nematode, whose adult forms are found among the intestinal microvilli of the water rat Nectomys squamipes, Brants 1827 (Rodentia: Muridae). The external appearance of the cuticle, which presents transversal striations and longitudinal ridges, is described using scanning electron mic...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-003-0845-2
更新日期：2003-07-01 00:00:00
abstract：:Maturation of trematode larval stages is expected to be temporally and spatially adapted to maximise the encounter with the adequate downstream host, i.e. the host, which will be infected by this parasite stage. Since studies on intramolluscan parasite maturation are scarce but important in the context of parasite tra...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-015-4774-7
更新日期：2016-02-01 00:00:00
abstract：:To mimic a human malaria infection in the endemic condition, two strains of mice (Balb/c and CBA) were infected and treated several times to generate so-called semi-immune status. As previously reported, neither mice (Balb/c and CBA) strain showed cerebral malaria, even in the susceptible C57BL/6 (B6). The significant...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-010-2121-6
更新日期：2011-04-01 00:00:00
abstract：:Loa loa, a filarial worm, can cause fatal encephalitis in humans. In an attempt to find alternatives to the standard treatments (ivermectin and diethylcarbamazine citrate), we tested 12 methanolic extracts of nine traditional plant remedies. The extracts (100-0.09 microg/ml) were incubated with 20 Loa loa microfilaria...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-010-1910-2
更新日期：2010-08-01 00:00:00
abstract：:Feasibility of implementing a DEC-fortified (DEC at 0.2% w/w and iodine) salt strategy to hasten elimination of diurnally sub-periodic Wuchereria bancrofti (DspWB) from the lone foci in Nancowry islands, Nicobar district, India, was assessed. This is a two-arm community-based study: one arm (12 villages, population 29...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-020-06659-7
更新日期：2020-05-01 00:00:00
abstract：:This analysis investigated the influence of breed and gender on the pharmacokinetics of monepantel, and influence of breed, age, and gender on its efficacy against gastrointestinal nematodes of sheep. In a comparison of pharmacokinetic profiles from two studies, Merino lambs had significantly greater maximum concentra...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-009-1671-y
更新日期：2010-01-01 00:00:00
abstract：:The free-living amoebae, Acanthamoeba, can act as opportunistic parasites on a wide range of vertebrates and are becoming a serious threat to human health due to the resistance of their cysts to harsh environmental conditions, disinfectants, some water treatment practices, and their ubiquitous distribution. Subgenus c...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-014-3945-2
更新日期：2014-08-01 00:00:00
abstract：:Although the life cycle of the equid stomach parasite Habronema muscae was disclosed more than 100 years ago, little is known about the effect of the developing nematode larvae in its intermediate host, Musca domestica. In a series of experiments, freshly hatched M. domestica larvae were exposed to H. muscae eggs cont...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-016-5312-y
更新日期：2017-02-01 00:00:00
abstract：:A human homologue of high mobility group box 1 (HMGB1) protein was cloned and characterized from the human filarial parasites Wuchereria bancrofti and Brugia malayi. Sequence analysis showed that W. bancrofti HMGB1 (WbHMGB1) and B. malayi HMGB1 (BmHMGB1) proteins share 99 % sequence identity. Filarial HMGB1 showed typ...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-012-2878-x
更新日期：2012-08-01 00:00:00
abstract：:The present work was aimed at evaluating the "in vitro" efficacy of different concentrations of thymol on engorged nymphs and females of Rhipicephalus sanguineus. The nymphs were separated in seven groups and immersed in different concentrations of thymol (0.25%, 0.5%, 1.0%, 1.5%, and 2.0%) for 5 min. A control group ...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-009-1530-x
更新日期：2009-10-01 00:00:00
abstract：:In this paper, we cloned a novel full-length cDNA that encodes a Trichinella spiralis cathepsin B-like protease gene (TsCPB) using 3'-RACE PCR. The recombinant mature TsCPB protein (rTsCPB) was then expressed in an Escherichia coli expression system and purified with Ni-affinity chromatography. Real-time quantitative ...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-013-3497-x
更新日期：2013-09-01 00:00:00
abstract：:From March to October 2003, a total of 2,518 host-seeking Ixodes ricinus ticks (1,944 nymphs, 264 females, 310 males) were collected by blanket dragging at 45 sites all over the city area of Bonn, western Germany, to be checked for Borrelia burgdorferi infection. The collection sites included 20 private gardens, nine ...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-004-1240-3
更新日期：2005-01-01 00:00:00
abstract：:Seventy perch (Perca fluviatilis) caught in Lake Constance at Langenargen in May 1990 were examined for parasites. The frequency distributions of the eyefluke Diplostomum spathaceum (Rudolphi, 1819), and the tapeworms Proteocephalus percae (Müller, 1780) and Triaenophorus nodulosus (Pallas, 1781) fitted closely to a n...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s004360050264
更新日期：1997-01-01 00:00:00
abstract：:Galectin plays an important role in host-parasite interactions. In this study, we identified a novel gene encoding galectin-10 (AcGal-10) from the cDNA library of Angiostrongylus cantonensis and characterized its biological role in the parasite. Sequence and phylogeny analysis showed that AcGal-10 is related to other ...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-012-3210-5
更新日期：2013-03-01 00:00:00
abstract：:Two neolignans, burchellin and nordihydroguaiaretic acid (NDGA), were toxic only to Trypanosoma cruzi clone Dm28c maintained in brain heart infusion (BHI) medium at a concentration of 100 microg/ml, not 10 microg/ml. When Rhodnius prolixus was fed with epimastigotes of T. cruzi and treated simultaneously with a single...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s004360050532
更新日期：1999-03-01 00:00:00
abstract：:Hepatic enzymes and biochemical parameter values were analysed in the sera of 32 lambs: 12 infected with 1,000 Dicrocoelium dendriticum metacercariae, 12 with 3,000 and eight controls. Blood samples were collected at intervals of 30 days from day 0 post-infection (p.i.). Half the lambs in each group were slaughtered o...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-004-1128-2
更新日期：2004-08-01 00:00:00
abstract：:The published data concerning Toxoplasma gondii, Hammondia hammondi, H. heydorni and Neospora caninum on one side and between T. gondii on the other were neglected by most authors. As conclusion we are convinced that there are only two valid species: Isospora (Toxoplasma) gondii and Hammondia heydorni. The first inclu...
journal_title：Parasitology research
pub_type： 杂志文章,评审
doi：10.1007/s004360050028
更新日期：2000-02-01 00:00:00
abstract：:The pathogenicity of 19 hypocrealean entomopathogenic fungi from seven different genera in adult Aedes aegypti was tested. All fungi proved to be pathogenic, and Isaria fumosorosea, Lecanicillium muscarium, Lecanicillium psalliotae, Metarhizium anisopliae, Metarhizium lepidiotae, Metarhizium majus, Metarhizium frigidu...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-010-1991-y
更新日期：2010-10-01 00:00:00
abstract：:This study identifies and characterizes one novel type of triactinospomyxon in oligochaete specimen of Branchiura sowerbyi Beddard collected from a fish pond used for rearing gibel carp located in Caidian Experimental Station of the Institute of Hydrobiology. It is nominated as Triactinospomyxon caidianensis type. The...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-011-2775-8
更新日期：2012-06-01 00:00:00
abstract：:Mosquitoes are the major vector for the transmission of malaria, dengue fever, yellow fever, filariasis, schistosomiasis, and Japanese encephalitis. Mosquito control is facing a threat because of the emergence of resistance to synthetic insecticides. Insecticides of botanical origin may serve as suitable alternative b...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-013-3709-4
更新日期：2014-02-01 00:00:00
abstract：:Adult worms that belong to Carassotrema Park 1938 and Elonginurus Lu, 1955 were found in the intestine of Carassius gibelio Bloch, 1782 from the southern Russian Far East and Mugil cephalus Linnaeus, 1758 from northern Vietnam, respectively. Morphometric parameters, geographic location and host species composition of ...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-019-06328-4
更新日期：2019-07-01 00:00:00
abstract：:This report presents the results of the first faunistic study of hard ticks in Qazvin province of Iran. The primary objective was to determine the species diversity and geographic distribution of hard ticks that parasitize domestic ruminants. Information about the abiotic preferences of these species has been provided...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-011-2501-6
更新日期：2012-01-01 00:00:00
abstract：:Toxoplasma gondii is an important zoonotic protozoan of the phylum Apicomplexa that can infect nearly all warm-blooded animals. The parasite can exist as the interconvertible tachyzoite or bradyzoite forms, leading to acute or latent infection, respectively. No drug has been reported to penetrate the cyst wall and red...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-020-06722-3
更新日期：2020-07-01 00:00:00
abstract：:Morphological abnormalities were observed on the cephalic bulb hooklets of advanced third-stage larvae (AdvL(3)) of genus Gnathostoma. The larvae were obtained from the fish "Mexican rivulus" Millerichthys robustus collected from a seasonal pond near Tlacotalpan, Veracruz, Mexico. The abnormalities involved (1) extra ...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-009-1604-9
更新日期：2009-11-01 00:00:00
