Abstract:
:Seven isolates of Cryptocaryon irritans from different host species and geographical locations in Mainland China were characterized by the first (ITS-1) and second (ITS-2) internal transcribed spacers (ITS) of nuclear ribosomal DNA (rDNA) using two isolates of Ichthyophthirius multifiliis for comparative purposes. The rDNA region including the ITS-1, 5.8S, ITS-2, and flanking 18S and 28S sequences were amplified by polymerase chain reaction and the amplicons were sequenced directly. The ITS-1, 5.8S, and ITS-2 sequences were 129, 160, and 190 bp in length, respectively, for all seven C. irritans isolates, whereas the corresponding sequences for the two I. multifiliis isolates were 142, 153, and 194 bp, respectively. While sequence variation among the seven C. irritans isolates ranged from 0 to 1.6% in both the ITS-1 and ITS-2, and the two I. multifiliis isolates differed by 1.4% in the ITS-1 and 1.0% in the ITS-2; C. irritans differed from I. multifiliis by 57.1-60.9% in the ITS-1 and 79.4-83.0% in the ITS-2, indicating that ITS sequences provide reliable genetic markers for the identification and differentiation of the two species. Phylogenetic analysis using the sequence pairwise-distance data using the neighbor-joining method inferred that the seven C. irritans isolates from Mainland China and two other isolates (T.A and Aus.C) from other countries clustered together to show monophyly, which could be readily distinguished from the other monophyletic group all from other regions. Therefore, ITS sequence data and phylogenetic analysis provided strong support that C. irritans isolates from Mainland China represent a single species. The definition of genetic markers in the ITS rDNA provide opportunities for studying the ecology and population genetic structures of the C. irritans from Mainland China and elsewhere and is also relevant to the diagnosis and control of fish diseases they cause.
journal_name
Parasitol Resjournal_title
Parasitology researchauthors
Sun HY,Zhu XQ,Xie MQ,Wu XY,Li AX,Lin RQ,Song HQdoi
10.1007/s00436-006-0151-xsubject
Has Abstractpub_date
2006-07-01 00:00:00pages
160-6issue
2eissn
0932-0113issn
1432-1955journal_volume
99pub_type
杂志文章abstract::Calcareous corpuscles, known as mineral concretions in Platyhelminthes, may have special biological functions. In the present study we succeeded in the quantitative separation of calcareous corpuscles in plerocercoids of Spirometra mansoni (sparganum) using Ficoll. Purified calcareous corpuscles were reacted with crud...
journal_title:Parasitology research
pub_type: 杂志文章
doi:10.1007/s004360000241
更新日期:2000-09-01 00:00:00
abstract::Rhodnius nasutus, a triatomine species autochthonous in the Brazilian Northeast, is primarily associated with the Copernicia prunifera palm tree (Carnauba). For the first time, the colonization of this triatomine in another tree species is reported. To investigate the existence of an infected triatomine focus located ...
journal_title:Parasitology research
pub_type: 杂志文章
doi:10.1007/s00436-007-0823-1
更新日期:2008-03-01 00:00:00
abstract::During the present investigation, alterations in the intrahepatic venous and portal systems in Rattus norvegicus infected with 250, 500, and 1000 Taenia taeniaeformis eggs were studied. The corrosion cast technique was employed, which provides an extrinsic opportunity for study of the three-dimensional orientation of ...
journal_title:Parasitology research
pub_type: 杂志文章
doi:10.1007/s004360050703
更新日期:2000-06-01 00:00:00
abstract::Cysticercoids as well as 6-, 10-, and 14-day Hymenolepis diminuta were evaluated in terms of enzymatic activities related to phosphoenolpyruvate (PEP) utilization and mitochondrial succinate accumulation. The data obtained support a transition toward anaerobic electron-transport-dependent succinate accumulation, chara...
journal_title:Parasitology research
pub_type: 杂志文章
doi:10.1007/s004360050487
更新日期:1998-10-01 00:00:00
abstract::The dynamics of production and excretion of leukotrienes by parasitic larvae of Oesophagostomum dentatum and their role for the development of the larvae were studied. Larvae were cultured in vitro to the fourth stage (L4). Leukotriene B4 (LTB4) was detected in homogenates and in supernatant of larvae, with the homoge...
journal_title:Parasitology research
pub_type: 杂志文章
doi:10.1007/s004360050138
更新日期:1996-01-01 00:00:00
abstract::Three new myxosporeans of the genus Sphaeromyxa Thélohan 1892 were discovered from the coastal waters off Xiamen in the East China Sea and characterized based on morphological and SSU rDNA data. Sphaeromyxa photopectoralis sp. n. was described from Photopectoralis bindus, and Sphaeromyxa sebastisca sp. n. was describe...
journal_title:Parasitology research
pub_type: 杂志文章
doi:10.1007/s00436-020-06656-w
更新日期:2020-05-01 00:00:00
abstract::The efficacy of emodepside plus toltrazuril (Procox® oral suspension for dogs) against different species of gastrointestinal nematodes (Toxocara canis, Ancylostoma caninum, Uncinaria stenocephala) was evaluated in nine randomised,blinded and placebo-controlled laboratory studies in naturally or experimentally infected...
journal_title:Parasitology research
pub_type: 杂志文章,随机对照试验
doi:10.1007/s00436-011-2397-1
更新日期:2011-08-01 00:00:00
abstract::Amebic cysteine protease 2 (EhCP2) was purified from ethyl ether extracts of axenically grown trophozoites of Entamoeba histolytica strain HM1-IMSS. The purification procedure involved molecular filtration and electroelution. Sequence analysis of the purified product revealed EhCP2 and ubiquitin(s). Electrophoretic mi...
journal_title:Parasitology research
pub_type: 杂志文章
doi:10.1007/s00436-002-0763-8
更新日期:2003-06-01 00:00:00
abstract::Trypanosomatidae is a family of unicellular parasites belonging to the phylum Euglenozoa, which are causative agents in high impact human diseases such as Leishmaniasis, Chagas disease and African sleeping sickness. The impact on human health and local economies, together with a lack of satisfactory chemotherapeutic t...
journal_title:Parasitology research
pub_type: 杂志文章
doi:10.1007/s00436-020-06779-0
更新日期:2020-09-01 00:00:00
abstract::The free-living amoebae, Acanthamoeba, can act as opportunistic parasites on a wide range of vertebrates and are becoming a serious threat to human health due to the resistance of their cysts to harsh environmental conditions, disinfectants, some water treatment practices, and their ubiquitous distribution. Subgenus c...
journal_title:Parasitology research
pub_type: 杂志文章
doi:10.1007/s00436-014-3945-2
更新日期:2014-08-01 00:00:00
abstract::Amoebic keratitis is difficult to treat without total efficacy in some patients because of cysts, which are less susceptible than trophozoites to the usual treatments. The aim of this study is to evaluate the in vitro amoebicidal activity of the methanolic extracts of Satureja cuneifolia and Melissa officinalis. In th...
journal_title:Parasitology research
pub_type: 杂志文章
doi:10.1007/s00436-011-2744-2
更新日期:2012-06-01 00:00:00
abstract::The utility of an enzyme-linked immunosorbent assay to determine the sensitization against the trematode Fasciola hepatica in horses from an endemic area (NW Spain) was assessed. Blood samples were collected from 536 horses and tested against a 2.9-kDa recombinant surface protein (FhrAPS) to estimate the presence of I...
journal_title:Parasitology research
pub_type: 杂志文章
doi:10.1007/s00436-011-2587-x
更新日期:2012-02-01 00:00:00
abstract::A study of development of Eimeria tenella in chickens fed high n-3 fatty acids (n-3FA) diets showed ultrastructural degeneration of both asexual and sexual parasite stages. Abnormal shedding of asexual and sexual parasite developmental stages into the cecal lumen was also observed. Ultrastructural degeneration was cha...
journal_title:Parasitology research
pub_type: 杂志文章
doi:10.1007/s004360050277
更新日期:1997-01-01 00:00:00
abstract::Light, scanning and transmission electron microscope examinations of adult Cystidicoloides ephemeridarum nematodes, parasitic in the stomach of brown trout (Salmo trutta fario L.), revealed that the body wall is composed of a cuticle, a hypodermis and longitudinally oriented somatic musculature. The body cuticle is co...
journal_title:Parasitology research
pub_type: 杂志文章
doi:10.1007/s00436-003-0935-1
更新日期:2003-09-01 00:00:00
abstract::Canine trypanosomosisis (CT) is a common disease caused by tsetse- and non-tsetse-transmitted trypanosomes worldwide. The severity of the disease varies from acute, sub-acute to chronic with non-specific clinical signs. Here, we attempt in a cross-sectional study to assess the current situation of CT and the role of d...
journal_title:Parasitology research
pub_type: 杂志文章
doi:10.1007/s00436-017-5598-4
更新日期:2017-11-01 00:00:00
abstract::Our previous in vivo studies on angiostrongyliasis showed that PF1022A had stronger killing effects against female adults than against males. No killing effects were observed against young adult worms in the central nervous system. To characterize the former in vivo action of PF1022A, in vitro effects of PF1022A on th...
journal_title:Parasitology research
pub_type: 杂志文章
doi:10.1007/s004360050301
更新日期:1997-01-01 00:00:00
abstract::The chemotherapeutic interventions against visceral leishmaniasis (VL) are limited and facing serious concerns of toxicity, high cost, and emerging drug resistance. There is a greater interest in new drug developments from traditionally used medicinal plants which offers unprecedented diversity in structures and bioac...
journal_title:Parasitology research
pub_type: 杂志文章
doi:10.1007/s00436-007-0822-2
更新日期:2008-02-01 00:00:00
abstract::Entamoeba histolytica infection in humans is still one of the leading causes of parasitic diseases among the developing countries, including India. It therefore becomes extremely important to characterize this parasite with the aim of preventing and controlling the amoebiasis which it causes. The present study describ...
journal_title:Parasitology research
pub_type: 杂志文章
doi:10.1007/s004360000363
更新日期:2002-02-01 00:00:00
abstract::Seven proprietary repellent formulations (3 hydro-alcoholic spray solutions and 4 skin lotions) with active ingredient IR3,535 (ethyl butylacetylaminopropionate, EBAAP) or Picaridin (hydroxyethyl isobutyl piperidine carboxylate, KBR 3,023, Bayrepel) were tested in a field study on 10 test persons over a period of 10 h...
journal_title:Parasitology research
pub_type: 杂志文章
doi:10.1007/s00436-006-0450-2
更新日期:2007-06-01 00:00:00
abstract::Companion animals including dogs are one of the important components in One Health. Parasites may cause not only diseases in pet animals but also many zoonotic diseases infecting humans. In this study, we performed a survey of intestinal parasites in fecal specimens (n = 485) collected from outpatient pet dogs with di...
journal_title:Parasitology research
pub_type: 杂志文章
doi:10.1007/s00436-017-5631-7
更新日期:2018-01-01 00:00:00
abstract::Human colonisation of New Zealand has resulted in the introduction of emerging diseases, such as avian malaria and toxoplasmosis, which arrived with their exotic avian and mammalian hosts. Plasmodium spp. and Toxoplasma gondii have a wide host range, and several species of endemic New Zealand birds have developed a fa...
journal_title:Parasitology research
pub_type: 杂志文章
doi:10.1007/s00436-017-5536-5
更新日期:2017-08-01 00:00:00
abstract::The larvicidal activities of methanolic fractions from Adhatoda vasica leaf extracts were investigated against the bancroftian filariasis vector Culex quinquefasciatus and dengue vector Aedes aegypti. The results indicated that the mortality rates was high at 100, 150, 200 and 250 ppm of methanol extract of fractions ...
journal_title:Parasitology research
pub_type: 杂志文章
doi:10.1007/s00436-011-2728-2
更新日期:2012-05-01 00:00:00
abstract::During our investigations on helminthofauna in roe deer (Capreolus capreolus) and red deer (Cervus elaphus) in France (1985-1998) we isolated nematodes not only of the genus Haemonchus Cobb, 1898 but also of the genus Ashworthius Le Roux 1930, both of which belong to the same subfamily of Haemonchinae. The prevalence ...
journal_title:Parasitology research
pub_type: 杂志文章
doi:10.1007/pl00008534
更新日期:2000-07-01 00:00:00
abstract::Because benznidazole (BZL) was found to downregulate nitric oxide (NO) and cytokine synthesis by murine macrophages, we analyzed the potential immunological repercussions of BZL treatment in Trypanosoma cruzi-infected rats. To evaluate whether the effects of BZL were also observed in the presence of an immunostimulati...
journal_title:Parasitology research
pub_type: 杂志文章
doi:10.1007/s004360000357
更新日期:2001-07-01 00:00:00
abstract:ABTRACT:Structure of the helminth community of Megaleporinus obtusidens collected in Lake Guaíba was evaluated, and the results indicated that the diversity of helminth species was probably determined by fish behavior and eating habits. The influence of sex, weight, and standard length of hosts for parasitic indices wa...
journal_title:Parasitology research
pub_type: 杂志文章
doi:10.1007/s00436-018-5933-4
更新日期:2018-08-01 00:00:00
abstract::During infections, Giardia lamblia undergoes a continuous change of its major surface antigens, the variant-specific surface proteins (VSPs). Many studies on antigenic variation have been performed using G. lamblia clone GS/M-83-H7, which expresses surface antigen VSP H7. The present study was focused on the identific...
journal_title:Parasitology research
pub_type: 杂志文章
doi:10.1007/s004360100490
更新日期:2001-12-01 00:00:00
abstract::Research on the infection of snails by trematodes has been conducted in Europe for over a hundred years. The initial poor knowledge of the intra-molluscan stages of these parasites together with the difficulty of classifying them constituted a serious obstacle to the undertaking of integrated parasitological and malac...
journal_title:Parasitology research
pub_type: 杂志文章,评审
doi:10.1007/s00436-009-1462-5
更新日期:2009-08-01 00:00:00
abstract::Plasmodium berghei XAT is an irradiation-induced, permanent attenuated derivative from high-virulence P. berghei NK65. Monoclonal antibodies against XAT were developed. By immunofluorescence screening, one monoclonal antibody was identified that was reactive with XAT at the schizont stage but not with NK65 nor with an...
journal_title:Parasitology research
pub_type: 杂志文章
doi:10.1007/BF00535143
更新日期:1988-01-01 00:00:00
abstract::A quantitative colorimetric assay using the oxidation-reduction indicator resazurin was developed to measure cytotoxicity of compounds against the protozoan parasite Trypanosoma cruzi. This method is based on the detection of colorimetric changes caused by the oxidation (blue) and reduction (pink) capabilities of resa...
journal_title:Parasitology research
pub_type: 杂志文章
doi:10.1007/s00436-006-0126-y
更新日期:2006-07-01 00:00:00
abstract::Diagnosis of Encephalitozoon cuniculi infection in rabbits remains a major veterinary issue. ELISA or immunofluorescence assays are the current reference standards of serological tests. However, these conventional techniques suffer from a lack of accuracy for distinguishing active from past infections, as a positive s...
journal_title:Parasitology research
pub_type: 杂志文章
doi:10.1007/s00436-016-5343-4
更新日期:2017-02-01 00:00:00