当前位置： SCI文献检索 > PARASITOLOGY RESEARCH期刊下所有文献 > Entomopathogenic marine actinomycetes as potential and low-cost biocontrol agents against bloodsucking arthropods.

Entomopathogenic marine actinomycetes as potential and low-cost biocontrol agents against bloodsucking arthropods.

Abstract：

:A novel approach to control strategies for integrated blood-feeding parasite management is in high demand, including the use of biological control agents. The present study aims to determine the efficacy of optimized crude extract of actinomycetes strain LK1 as biological control agent against the fourth-instar larvae of Anopheles stephensi and Culex tritaeniorhynchus (Diptera: Culicidae) and adults of Haemaphysalis bispinosa, Rhipicephalus (Boophilus) microplus (Acari: Ixodidae), and Hippobosca maculata (Diptera: Hippoboscidae). Antiparasitic activity was optimized using the Plackett-Burman method, and the design was developed using the software Design-Expert version 8.0.7.1. The production of the optimized crude actinomycetes LK1 strain extract was performed using response surface methodology to optimize the process parameters of protease inhibitor activity of marine actinobacteria for the independent variables like pH, temperature, glucose, casein, and NaCl at two levels (-1 and +1). The potential actinomycetes strain was identified as Saccharomonas spp., and the metamodeling surface simulation procedure was followed. It was studied using a computer-generated experimental design, automatic control of simulation experiments, and sequential optimization of the metamodels fitted to a simulation response surface function. The central composite design (CCD) used for the analysis of treatment showed that a second-order polynomial regression model was in good agreement with the experimental results at R (2) = 0.9829 (p < 0.05). The optimized values of the variables for antioxidant production were pH 6.00, glucose 1.3%, casein 0.09%, temperature 31.23 °C, and NaCl 0.10%. The LK1 strain-optimized crude extract was purified using reversed-phase high-pressure liquid chromatography, and the isolated protease inhibitor showed antiparasitic activity. The antiparasitic activity of optimized crude extract of LK1 was tested against larvae of A. stephensi (LC₅₀ = 31.82 ppm; r(2) = 0.818) and C. tritaeniorhynchus (LC₅₀ = 26.62 ppm; r(2) = 0.790) and adults of H. bispinosa (LC₅₀ = 106.58 ppm; r(2) = 0.871), R. (B.) microplus (LC₅₀ = 92.96 ppm; r(2) = 0.913), and H. maculata (LC₅₀ = 84.90 ppm; r(2) = 0.857).

journal_name

Parasitol Res

journal_title

Parasitology research

authors

Loganathan K,Kumar G,Kirthi AV,Rao KV,Rahuman AA

doi

10.1007/s00436-013-3585-y

subject

Has Abstract

pub_date

2013-11-01 00:00:00

pages

3951-9

issue

eissn

0932-0113

issn

1432-1955

journal_volume

112

pub_type

杂志文章

在线工具

Entomopathogenic marine actinomycetes as potential and low-cost biocontrol agents against bloodsucking arthropods.