Trypanosoma cruzi: evaluation of (-)-cubebin derivatives activity in the messenger RNAs processing.


:No fully effective treatment has been developed since the discovery of Chagas' disease. Since drug-resistant Trypanosoma cruzi strains are occurring and the current therapy is effective in the acute phase but with various adverse side effects, more studies are needed to characterize the susceptibility of T. cruzi to new drugs. Pre-mRNA maturation in trypanosomatids occurs through a process called trans-splicing, which is unusual RNA processing reaction, and it implies the processing of polycistronic transcription units into individual mRNAs; a short transcript spliced leader (SL RNA) is trans-spliced to the acceptor pre-mRNA, giving origin to the mature mRNA. Cubebin derivatives seem to provide treatments with less collateral effects than benznidazole and showed similar or better trypanocidal activities than benznidazole. Therefore, the cubebin derivatives ((-)-6,6'-dinitrohinokinin (DNH) and (-)-hinokinin (HQ)) interference in the mRNA processing was evaluated using T. cruzi permeable cells (Y and BOL (Bolivia) strains) following by RNase protection reaction. These substances seem to intervene in any step of the RNA transcription, promoting alterations in the RNA synthesis, even though the RNA processing mechanism still occurs. Furthermore, HQ presented better activity against the parasites than DNH, meaning that BOL strain seems to be more resistant than Y.


Parasitol Res


Parasitology research


Andrade e Silva ML,Cicarelli RM,Pauletti PM,Luz PP,Rezende KC,Januário AH,da Silva R,Pereira AC,Bastos JK,de Albuquerque S,Magalhães LG,Cunha WR




Has Abstract


2011-08-01 00:00:00












  • Enzyme-linked immunosorbent assays for the detection of equine antibodies specific to a recombinant Fasciola hepatica surface antigen in an endemic area.

    abstract::The utility of an enzyme-linked immunosorbent assay to determine the sensitization against the trematode Fasciola hepatica in horses from an endemic area (NW Spain) was assessed. Blood samples were collected from 536 horses and tested against a 2.9-kDa recombinant surface protein (FhrAPS) to estimate the presence of I...

    journal_title:Parasitology research

    pub_type: 杂志文章


    authors: Arias MS,Piñeiro P,Hillyer GV,Francisco I,Cazapal-Monteiro CF,Suárez JL,Morrondo P,Sánchez-Andrade R,Paz-Silva A

    更新日期:2012-02-01 00:00:00

  • Identification of stage-specific proteins of Angiostrongylus vasorum (Baillet, 1866) Kamensky.

    abstract::Sera of ten dogs infected with Angiostrongylus vasorum were analyzed by immunoblotting proteins of first stage larvae (L1) and adult parasites. The molecular weights (m.w.) of the principal L1 proteins identified by IgG, IgM, IgA, and IgE were 18-118 kDa and those of the adult parasite were 28-209 kDa. The L1 proteins...

    journal_title:Parasitology research

    pub_type: 杂志文章


    authors: De Oliveira Vasconcelos V,De Almeida Vitor RW,Dos Santos Lima W

    更新日期:2008-02-01 00:00:00

  • Interaction of BOP1, a protein for ribosome biogenesis, with EB1 in Giardia lamblia.

    abstract::A search for a protein(s) interacting with the block of proliferation 1 (Bop1) protein of Giardia lamblia led to the isolation of end binding (EB1) protein 1, a protein that is known to function as a microtubule (MT) plus-end-tracking protein in other organisms. Interaction between Bop1 and EB1 of G. lamblia was confi...

    journal_title:Parasitology research

    pub_type: 杂志文章


    authors: Kim J,Sim S,Yong TS,Park SJ

    更新日期:2008-11-01 00:00:00

  • Detection of Blastocystis from stool samples using real-time PCR.

    abstract::We developed a real-time LC PCR assay to detect a 152 bp sequence in an uncharacterized region of the Blastocystis genome. The described assay detected 11 of 11 ATCC strains of Blastocystis from subtypes 1, 3, and 4. Three of three stool samples from Oregon and California military personnel that were negative for Blas...

    journal_title:Parasitology research

    pub_type: 杂志文章


    authors: Jones MS 2nd,Ganac RD,Hiser G,Hudson NR,Le A,Whipps CM

    更新日期:2008-08-01 00:00:00

  • Strains of Toxoplasma gondii used for tachyzoite antigens to stimulate spleen cells of infected mice in vitro affect cytokine responses of the cells in the culture.

    abstract::Cytokine responses of lymphocytes against Toxoplasma gondii are usually studied by stimulating the cells from infected animals with tachyzoite lysate antigens (TLA) in vitro. We examined whether strains (genotypes) of the parasite used for TLA affect the production of IL-2, IL-4, IL-10 and IFN-gamma in the culture of ...

    journal_title:Parasitology research

    pub_type: 杂志文章


    authors: Rodgers L,Wang X,Wen X,Dunford B,Miller R,Suzuki Y

    更新日期:2005-10-01 00:00:00

  • Protective immunity against Toxoplasma gondii in mice induced by the SAG2 internal image of anti-idiotype antibody.

    abstract::A monoclonal antibody TGCM5 (isotype G, subclass 1, kappa light chain) against SAG2, a major surface antigen of Toxoplasma gondii tachyzoites, was produced in quantity and its Fab-containing idiotype (Id) was injected into rabbits for production of rabbit anti-Id sera. The rabbit anti-Id IgG (aId-IgG) with a SAG2 inte...

    journal_title:Parasitology research

    pub_type: 杂志文章


    authors: Yang CD,Chang GN,Chao D

    更新日期:2003-12-01 00:00:00

  • An enzyme-linked immunosorbent assay for detection of Theileria parva antibodies in cattle using a recombinant polymorphic immunodominant molecule.

    abstract::Field and experimental bovine infection sera were used in immunoblots of sporozoite and schizont lysates of Theileria parva to identify candidate diagnostic antigens. Four parasite antigens of Mr 67,000 (p67), 85,000 (the polymorphic immunodominant molecule, PIM), 104,000 (p104), and 150,000 (p150) were selected for a...

    journal_title:Parasitology research

    pub_type: 杂志文章


    authors: Katende J,Morzaria S,Toye P,Skilton R,Nene V,Nkonge C,Musoke A

    更新日期:1998-05-01 00:00:00

  • Outbreak of toxoplasmosis in a flock of domestic chickens (Gallus gallus domesticus) and guinea fowl (Numida meleagris).

    abstract::Toxoplasmosis is a disease with a worldwide distribution that affects a wide variety of animal species, though with rare descriptions in chickens. We describe the clinical, epidemiological, pathological, and molecular aspects of a toxoplasmosis outbreak in domestic chickens and guinea fowl in southern Brazil. The floc...

    journal_title:Parasitology research

    pub_type: 杂志文章


    authors: Vielmo A,Pena HFJ,Panziera W,Bianchi RM,De Lorenzo C,Oliveira S,Alves BF,Gennari SM,Pavarini SP,de Barros CSL,Driemeier D

    更新日期:2019-03-01 00:00:00

  • In silico cloning and B/T cell epitope prediction of triosephosphate isomerase from Echinococcus granulosus.

    abstract::Cystic echinococcosis is a worldwide zoonosis caused by Echinococcus granulosus. Because the methods of diagnosis and treatment for cystic echinococcosis were limited, it is still necessary to screen target proteins for the development of new anti-hydatidosis vaccine. In this study, the triosephosphate isomerase gene ...

    journal_title:Parasitology research

    pub_type: 杂志文章


    authors: Wang F,Ye B

    更新日期:2016-10-01 00:00:00

  • Survivability of Kudoa septempunctata in human intestinal conditions.

    abstract::To elucidate whether Kudoa septempunctata was able to live in the human intestine, we assessed viability of K. septempunctata sporoplasms under conditions that mimicked human and ragworm digestive tracts. To study the effect of osmotic pressure on viability, sporoplasms were incubated in 0.9 or 3.4 % sodium chloride s...

    journal_title:Parasitology research

    pub_type: 杂志文章


    authors: Ohnishi T,Fujiwara M,Tomaru A,Yoshinari T,Sugita-Konishi Y

    更新日期:2016-06-01 00:00:00

  • Molecular identification of Echinococcus species from eastern and southern Qinghai, China, based on the mitochondrial cox1 gene.

    abstract::The Qinghai-Tibetan Plateau (QTP, in western China), which is the largest and highest plateau on Earth, is a highly epidemic region for Echinococcus spp. We collected 70 Echinococcus samples from humans, dogs, sheep, yaks, plateau pikas, and voles in eastern and southern Qinghai and genotyped them using the mitochondr...

    journal_title:Parasitology research

    pub_type: 杂志文章


    authors: Ma J,Wang H,Lin G,Craig PS,Ito A,Cai Z,Zhang T,Han X,Ma X,Zhang J,Liu Y,Zhao Y,Wang Y

    更新日期:2012-07-01 00:00:00

  • The effects of artemether-lumefantrine vs amodiaquine-sulfalene-pyrimethamine on the hepatomegaly associated with Plasmodium falciparum malaria in children.

    abstract::An open randomized controlled study of artemether-lumefantrine (AL) and amodiaquine-sulfalene-pyrimethamine (ASP) for the treatment of uncomplicated Plasmodium falciparum malaria was carried out in 181 children. In 79 children, the hepatomegaly reduction ratios (HRR) and the speed of resolution of hepatomegaly, the he...

    journal_title:Parasitology research

    pub_type: 杂志文章,随机对照试验


    authors: Sowunmi A,Gbotosho GO,Adedeji AA,Tambo E,Fateye BA,Happi CT,Amoo AO

    更新日期:2007-02-01 00:00:00

  • A scanning electron microscopy study of early development in vitro of Contracaecum multipapillatum s.l. (Nematoda: Anisakidae) from a brown pelican (Pelecanus occidentalis) from the Gulf of California, Mexico.

    abstract::Eggs obtained from the uteri of female nematodes, genetically identified as Contracaecum multipapillatum s.l., found in a brown pelican (Pelecanus occidentalis) from Bahía de La Paz, Gulf of California, Mexico, were used to study the early developmental stages of this anisakid by scanning electron microscopy (SEM). Eg...

    journal_title:Parasitology research

    pub_type: 杂志文章


    authors: Molina-Fernández D,Valles-Vega I,Hernández-Trujillo S,Adroher FJ,Benítez R

    更新日期:2017-10-01 00:00:00

  • Coccidia (Apicomplexa: Eimeriidae) of elk (Alces alces) in Poland.

    abstract::Over a 4-year period, we analyzed 128 fecal samples from free-living elk (Alces alces L., 1758) to determine the prevalence of Eimeria infections and identify the species present. Two eimerian species were isolated including Eimeria alces and a morphotype resembling Eimeria catubrina. Overall, two samples from 128 sam...

    journal_title:Parasitology research

    pub_type: 杂志文章


    authors: Pyziel AM,Demiaszkiewicz AW

    更新日期:2013-05-01 00:00:00

  • Response to repeated inoculations with Ascaris suum eggs in pigs during the fattening period. I. Studies on worm population kinetics.

    abstract::This experimental study on pigs was designed to simulate natural, long-term exposure to Ascaris suum under modern management conditions. Parasite kinetics were followed in pigs receiving A. suum eggs as repeated trickle inoculations at two dose levels beginning at a body weight of 25 kg until their slaughter at 90 kg ...

    journal_title:Parasitology research

    pub_type: 杂志文章


    authors: Eriksen L,Nansen P,Roepstorff A,Lind P,Nilsson O

    更新日期:1992-01-01 00:00:00

  • Spermatological characters of bothriocephalideans (Cestoda) inferred from an ultrastructural study on Oncodiscus sauridae and Senga sp.

    abstract::Spermiogenesis and ultrastructure of the spermatozoon of two bothriocephalidean cestodes, Oncodiscus sauridae from the lizardfish Saurida nebulosa Valenciennes, 1850 and Senga sp. from the eel Mastacembelus armatus (Lacepède, 1800), have been studied using transmission electron microscopy. Spermiogenesis included the ...

    journal_title:Parasitology research

    pub_type: 杂志文章


    authors: Sípková L,Levron C,Oros M,Justine JL

    更新日期:2011-07-01 00:00:00

  • Fine structure of uterus and non-functioning paruterine organ in Orthoskrjabinia junlanae (Cestoda, Cyclophyllidea).

    abstract::Some cyclophyllidean cestodes provide protection for their eggs in the external environment by providing them with additional protective layers around the egg membranes. In attempting to examine such adaptations, the microanatomy and fine structure of the uterus of pregravid and gravid proglottids of the cyclophyllide...

    journal_title:Parasitology research

    pub_type: 杂志文章


    authors: Korneva JV,Kornienko SA,Jones MK

    更新日期:2016-06-01 00:00:00

  • Analysis of a novel cathepsin B circulating antigen and its response to drug treatment in Trichinella-infected mice.

    abstract::In this paper, we cloned a novel full-length cDNA that encodes a Trichinella spiralis cathepsin B-like protease gene (TsCPB) using 3'-RACE PCR. The recombinant mature TsCPB protein (rTsCPB) was then expressed in an Escherichia coli expression system and purified with Ni-affinity chromatography. Real-time quantitative ...

    journal_title:Parasitology research

    pub_type: 杂志文章


    authors: Zhan JH,Yao JP,Liu W,Hu XC,Wu ZD,Zhou XW

    更新日期:2013-09-01 00:00:00

  • Mitochondrial haplotype diversity of Gyrodactylus thymalli (Platyhelminthes; Monogenea): extended geographic sampling in United Kingdom, Poland, and Norway reveals further lineages.

    abstract::In recent years, the mitochondrial haplotype diversity of the monogenean ectoparasites Gyrodactylus salaris Malmberg, 1957 on Atlantic salmon and G. thymalli Zitnan, 1960 on grayling has been studied intensively to understand the taxonomy and phylogeography of the two species. According to these studies, neither speci...

    journal_title:Parasitology research

    pub_type: 杂志文章


    authors: Hansen H,Bakke TA,Bachmann L

    更新日期:2007-05-01 00:00:00

  • Contamination of the environment by strongylid (Nematoda: Strongylidae) infective larvae at horse farms of various types in Ukraine.

    abstract::Analysis of the influence of horse-keeping conditions by contamination of the environment (pastures, paddocks, and stalls) by the strongylid infective larvae (L(3)) was carried out at various types of horse farms, hippodromes, and riding clubs in Ukraine. A total of 1,237 horses from three types of horse-keeping condi...

    journal_title:Parasitology research

    pub_type: 杂志文章


    authors: Kuzmina TA

    更新日期:2012-05-01 00:00:00

  • Caryospora cherrughi sp. n. (Apicomplexa: Eimeriidae) infecting Falco cherrug in Saudi Arabia.

    abstract::The oocysts of Caryospora cherrughi sp. n. were described from the feces of the saker falcon, Falco cherrug, collected from the falcon market in Riyadh City, Saudi Arabia. The prevalence of infection was 21.4 % (15/70). The majority of the oocysts examined had completed sporulation within 64-84 h at 24 ± 2 °C. Sporula...

    journal_title:Parasitology research

    pub_type: 杂志文章


    authors: Alfaleh FA,Alyousif MS,Al-Shawa YR,Al-Quraishy S

    更新日期:2013-03-01 00:00:00

  • Elimination of diurnally sub-periodic Wuchereria bancrofti in Andaman and Nicobar Islands, India, using mass DEC-fortified salt as a supplementary intervention to MDA.

    abstract::Feasibility of implementing a DEC-fortified (DEC at 0.2% w/w and iodine) salt strategy to hasten elimination of diurnally sub-periodic Wuchereria bancrofti (DspWB) from the lone foci in Nancowry islands, Nicobar district, India, was assessed. This is a two-arm community-based study: one arm (12 villages, population 29...

    journal_title:Parasitology research

    pub_type: 杂志文章


    authors: Shriram AN,Premkumar A,Krishnamoorthy K,De A,Paul SK,Subramanian S,Vijayachari P,Jambulingam P

    更新日期:2020-05-01 00:00:00

  • Screening the Schistosoma mansoni transcriptome for genes differentially expressed in the schistosomulum stage in search for vaccine candidates.

    abstract::Schistosomiasis affects more than 200 million people worldwide; another 600 million are at risk of infection. The schistosomulum stage is believed to be the target of protective immunity in the attenuated cercaria vaccine model. In an attempt to identify genes up-regulated in the schistosomulum stage in relation to ce...

    journal_title:Parasitology research

    pub_type: 杂志文章


    authors: Farias LP,Tararam CA,Miyasato PA,Nishiyama MY Jr,Oliveira KC,Kawano T,Verjovski-Almeida S,Leite LC

    更新日期:2011-01-01 00:00:00

  • Primary structure of a BiP homologue in Eimeria spp.

    abstract::cDNA clones for homologues of a molecular chaperone of the endoplasmic reticulum called the immunoglobulin heavy-chain binding protein (BiP) have been isolated from Eimeria maxima and E. tenella sporozoite cDNA libraries. The E. tenella cDNA clone is of full length and has a predicted N-terminal signal sequence of app...

    journal_title:Parasitology research

    pub_type: 杂志文章


    authors: Dunn PP,Bumstead JM,Tomley FM

    更新日期:1996-01-01 00:00:00

  • In vivo and in vitro experimental intestinal amebiasis in Mongolian gerbils (Meriones unguiculatus).

    abstract::One of the main drawbacks of experimental amebiasis is the lack of an adequate animal model for invasive intestinal lesions. Mongolian gerbils are useful because both intestinal and hepatic amebiasis can be produced experimentally with Entamoeba histolytica trophozoites. In this paper we show results obtained with in ...

    journal_title:Parasitology research

    pub_type: 杂志文章


    authors: Shibayama M,Navarro-García F,López-Revilla R,Martínez-Palomo A,Tsutsumi V

    更新日期:1997-01-01 00:00:00

  • Echinococcus multilocularis metacestode metabolites contain a cysteine protease that digests eotaxin, a CC pro-inflammatory chemokine.

    abstract::In many helminthic infections, eotaxin, a CC-chemokine, triggers the mobilization of eosinophils, thus, contributing to an elevated blood and periparasitic eosinophil level. Following an experimental intraperitoneal infection of C57BL6 mice with Echinococcus multilocularis metacestodes, however, we observed the absenc...

    journal_title:Parasitology research

    pub_type: 杂志文章


    authors: Mejri N,Gottstein B

    更新日期:2009-10-01 00:00:00

  • Seasonal dynamics of Trichodina spp. on whiting (Merlangius merlangus)in relation to organic pollution on the eastern Black Sea coast of Turkey.

    abstract::It was determined that there is a relationship between prevalence and mean densities of Trichodina spp. on Merlangius merlangus and organic pollution, measured as levels of nitrite, nitrate and phosphate in the surrounding environment. Monthly, two left anterior gill arcs of 60 M. merlangus (unless otherwise stated) c...

    journal_title:Parasitology research

    pub_type: 杂志文章


    authors: Ogut H,Palm HW

    更新日期:2005-06-01 00:00:00

  • Sarcocystis neurona reacting antibodies in Missouri feral domestic cats (Felis domesticus) and their role as an intermediate host.

    abstract::Sarcocystis neurona is the parasite associated with equine protozoal myeloencephalitis (EPM). Recently, cats (Felis domesticus) have been implicated as a potential intermediate host in the life cycle of S. neurona. This study was initiated to determine whether cats have antibodies that react to S. neurona antigens sim...

    journal_title:Parasitology research

    pub_type: 杂志文章


    authors: Turay HO,Barr BC,Caldwell A,Branson KR,Cockrell MK,Marsh AE

    更新日期:2002-01-01 00:00:00

  • Inhibitory effect of allicin on the growth of Babesia and Theileria equi parasites.

    abstract::Allicin is an active ingredient of garlic that has antibacterial, antifungal, antiviral, and antiprotozoal activity. However, the inhibitory effects of allicin on Babesia parasites have not yet been examined. In the present study, allicin was tested as a potent inhibitor against the in vitro growth of bovine and equin...

    journal_title:Parasitology research

    pub_type: 杂志文章


    authors: Salama AA,AbouLaila M,Terkawi MA,Mousa A,El-Sify A,Allaam M,Zaghawa A,Yokoyama N,Igarashi I

    更新日期:2014-01-01 00:00:00

  • Evora district as a new focus for canine leishmaniasis in Portugal.

    abstract::On the basis of information acquired from local health authorities in Evora district of Portugal on cases of visceral leishmaniasis (VL), an epidemiology survey study was conducted. To determine the prevalence of anti-Leishmania antibodies in the local human and canine populations residing in Evora town and 14 adjacen...

    journal_title:Parasitology research

    pub_type: 杂志文章


    authors: Semião-Santos SJ,el Harith A,Ferreira E,Pires CA,Sousa C,Gusmão R

    更新日期:1995-01-01 00:00:00