Abstract：
:No fully effective treatment has been developed since the discovery of Chagas' disease. Since drug-resistant Trypanosoma cruzi strains are occurring and the current therapy is effective in the acute phase but with various adverse side effects, more studies are needed to characterize the susceptibility of T. cruzi to new drugs. Pre-mRNA maturation in trypanosomatids occurs through a process called trans-splicing, which is unusual RNA processing reaction, and it implies the processing of polycistronic transcription units into individual mRNAs; a short transcript spliced leader (SL RNA) is trans-spliced to the acceptor pre-mRNA, giving origin to the mature mRNA. Cubebin derivatives seem to provide treatments with less collateral effects than benznidazole and showed similar or better trypanocidal activities than benznidazole. Therefore, the cubebin derivatives ((-)-6,6'-dinitrohinokinin (DNH) and (-)-hinokinin (HQ)) interference in the mRNA processing was evaluated using T. cruzi permeable cells (Y and BOL (Bolivia) strains) following by RNase protection reaction. These substances seem to intervene in any step of the RNA transcription, promoting alterations in the RNA synthesis, even though the RNA processing mechanism still occurs. Furthermore, HQ presented better activity against the parasites than DNH, meaning that BOL strain seems to be more resistant than Y.
journal_name
Parasitol Resjournal_title
Parasitology researchauthors
Andrade e Silva ML,Cicarelli RM,Pauletti PM,Luz PP,Rezende KC,Januário AH,da Silva R,Pereira AC,Bastos JK,de Albuquerque S,Magalhães LG,Cunha WRdoi
10.1007/s00436-011-2275-xsubject
Has Abstractpub_date
2011-08-01 00:00:00pages
445-51issue
2eissn
0932-0113issn
1432-1955journal_volume
109pub_type
杂志文章abstract：:The utility of an enzyme-linked immunosorbent assay to determine the sensitization against the trematode Fasciola hepatica in horses from an endemic area (NW Spain) was assessed. Blood samples were collected from 536 horses and tested against a 2.9-kDa recombinant surface protein (FhrAPS) to estimate the presence of I...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-011-2587-x
更新日期：2012-02-01 00:00:00
abstract：:Sera of ten dogs infected with Angiostrongylus vasorum were analyzed by immunoblotting proteins of first stage larvae (L1) and adult parasites. The molecular weights (m.w.) of the principal L1 proteins identified by IgG, IgM, IgA, and IgE were 18-118 kDa and those of the adult parasite were 28-209 kDa. The L1 proteins...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-007-0774-6
更新日期：2008-02-01 00:00:00
abstract：:A search for a protein(s) interacting with the block of proliferation 1 (Bop1) protein of Giardia lamblia led to the isolation of end binding (EB1) protein 1, a protein that is known to function as a microtubule (MT) plus-end-tracking protein in other organisms. Interaction between Bop1 and EB1 of G. lamblia was confi...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-008-1131-0
更新日期：2008-11-01 00:00:00
abstract：:We developed a real-time LC PCR assay to detect a 152 bp sequence in an uncharacterized region of the Blastocystis genome. The described assay detected 11 of 11 ATCC strains of Blastocystis from subtypes 1, 3, and 4. Three of three stool samples from Oregon and California military personnel that were negative for Blas...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-008-1006-4
更新日期：2008-08-01 00:00:00
abstract：:Cytokine responses of lymphocytes against Toxoplasma gondii are usually studied by stimulating the cells from infected animals with tachyzoite lysate antigens (TLA) in vitro. We examined whether strains (genotypes) of the parasite used for TLA affect the production of IL-2, IL-4, IL-10 and IFN-gamma in the culture of ...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-005-1416-5
更新日期：2005-10-01 00:00:00
abstract：:A monoclonal antibody TGCM5 (isotype G, subclass 1, kappa light chain) against SAG2, a major surface antigen of Toxoplasma gondii tachyzoites, was produced in quantity and its Fab-containing idiotype (Id) was injected into rabbits for production of rabbit anti-Id sera. The rabbit anti-Id IgG (aId-IgG) with a SAG2 inte...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-003-1006-3
更新日期：2003-12-01 00:00:00
abstract：:Field and experimental bovine infection sera were used in immunoblots of sporozoite and schizont lysates of Theileria parva to identify candidate diagnostic antigens. Four parasite antigens of Mr 67,000 (p67), 85,000 (the polymorphic immunodominant molecule, PIM), 104,000 (p104), and 150,000 (p150) were selected for a...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s004360050419
更新日期：1998-05-01 00:00:00
abstract：:Toxoplasmosis is a disease with a worldwide distribution that affects a wide variety of animal species, though with rare descriptions in chickens. We describe the clinical, epidemiological, pathological, and molecular aspects of a toxoplasmosis outbreak in domestic chickens and guinea fowl in southern Brazil. The floc...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-019-06233-w
更新日期：2019-03-01 00:00:00
abstract：:Cystic echinococcosis is a worldwide zoonosis caused by Echinococcus granulosus. Because the methods of diagnosis and treatment for cystic echinococcosis were limited, it is still necessary to screen target proteins for the development of new anti-hydatidosis vaccine. In this study, the triosephosphate isomerase gene ...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-016-5166-3
更新日期：2016-10-01 00:00:00
abstract：:To elucidate whether Kudoa septempunctata was able to live in the human intestine, we assessed viability of K. septempunctata sporoplasms under conditions that mimicked human and ragworm digestive tracts. To study the effect of osmotic pressure on viability, sporoplasms were incubated in 0.9 or 3.4 % sodium chloride s...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-016-5036-z
更新日期：2016-06-01 00:00:00
abstract：:The Qinghai-Tibetan Plateau (QTP, in western China), which is the largest and highest plateau on Earth, is a highly epidemic region for Echinococcus spp. We collected 70 Echinococcus samples from humans, dogs, sheep, yaks, plateau pikas, and voles in eastern and southern Qinghai and genotyped them using the mitochondr...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-012-2815-z
更新日期：2012-07-01 00:00:00
abstract：:An open randomized controlled study of artemether-lumefantrine (AL) and amodiaquine-sulfalene-pyrimethamine (ASP) for the treatment of uncomplicated Plasmodium falciparum malaria was carried out in 181 children. In 79 children, the hepatomegaly reduction ratios (HRR) and the speed of resolution of hepatomegaly, the he...
journal_title：Parasitology research
pub_type： 杂志文章,随机对照试验
doi：10.1007/s00436-006-0293-x
更新日期：2007-02-01 00:00:00
abstract：:Eggs obtained from the uteri of female nematodes, genetically identified as Contracaecum multipapillatum s.l., found in a brown pelican (Pelecanus occidentalis) from Bahía de La Paz, Gulf of California, Mexico, were used to study the early developmental stages of this anisakid by scanning electron microscopy (SEM). Eg...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-017-5583-y
更新日期：2017-10-01 00:00:00
abstract：:Over a 4-year period, we analyzed 128 fecal samples from free-living elk (Alces alces L., 1758) to determine the prevalence of Eimeria infections and identify the species present. Two eimerian species were isolated including Eimeria alces and a morphotype resembling Eimeria catubrina. Overall, two samples from 128 sam...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-012-3262-6
更新日期：2013-05-01 00:00:00
abstract：:This experimental study on pigs was designed to simulate natural, long-term exposure to Ascaris suum under modern management conditions. Parasite kinetics were followed in pigs receiving A. suum eggs as repeated trickle inoculations at two dose levels beginning at a body weight of 25 kg until their slaughter at 90 kg ...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/BF00931733
更新日期：1992-01-01 00:00:00
abstract：:Spermiogenesis and ultrastructure of the spermatozoon of two bothriocephalidean cestodes, Oncodiscus sauridae from the lizardfish Saurida nebulosa Valenciennes, 1850 and Senga sp. from the eel Mastacembelus armatus (Lacepède, 1800), have been studied using transmission electron microscopy. Spermiogenesis included the ...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-010-2215-1
更新日期：2011-07-01 00:00:00
abstract：:Some cyclophyllidean cestodes provide protection for their eggs in the external environment by providing them with additional protective layers around the egg membranes. In attempting to examine such adaptations, the microanatomy and fine structure of the uterus of pregravid and gravid proglottids of the cyclophyllide...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-016-4997-2
更新日期：2016-06-01 00:00:00
abstract：:In this paper, we cloned a novel full-length cDNA that encodes a Trichinella spiralis cathepsin B-like protease gene (TsCPB) using 3'-RACE PCR. The recombinant mature TsCPB protein (rTsCPB) was then expressed in an Escherichia coli expression system and purified with Ni-affinity chromatography. Real-time quantitative ...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-013-3497-x
更新日期：2013-09-01 00:00:00
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journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-006-0423-5
更新日期：2007-05-01 00:00:00
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journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-011-2684-x
更新日期：2012-05-01 00:00:00
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journal_title：Parasitology research
pub_type： 杂志文章
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更新日期：2013-03-01 00:00:00
abstract：:Feasibility of implementing a DEC-fortified (DEC at 0.2% w/w and iodine) salt strategy to hasten elimination of diurnally sub-periodic Wuchereria bancrofti (DspWB) from the lone foci in Nancowry islands, Nicobar district, India, was assessed. This is a two-arm community-based study: one arm (12 villages, population 29...
journal_title：Parasitology research
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更新日期：2020-05-01 00:00:00
abstract：:Schistosomiasis affects more than 200 million people worldwide; another 600 million are at risk of infection. The schistosomulum stage is believed to be the target of protective immunity in the attenuated cercaria vaccine model. In an attempt to identify genes up-regulated in the schistosomulum stage in relation to ce...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-010-2045-1
更新日期：2011-01-01 00:00:00
abstract：:cDNA clones for homologues of a molecular chaperone of the endoplasmic reticulum called the immunoglobulin heavy-chain binding protein (BiP) have been isolated from Eimeria maxima and E. tenella sporozoite cDNA libraries. The E. tenella cDNA clone is of full length and has a predicted N-terminal signal sequence of app...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s004360050163
更新日期：1996-01-01 00:00:00
abstract：:One of the main drawbacks of experimental amebiasis is the lack of an adequate animal model for invasive intestinal lesions. Mongolian gerbils are useful because both intestinal and hepatic amebiasis can be produced experimentally with Entamoeba histolytica trophozoites. In this paper we show results obtained with in ...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s004360050228
更新日期：1997-01-01 00:00:00
abstract：:In many helminthic infections, eotaxin, a CC-chemokine, triggers the mobilization of eosinophils, thus, contributing to an elevated blood and periparasitic eosinophil level. Following an experimental intraperitoneal infection of C57BL6 mice with Echinococcus multilocularis metacestodes, however, we observed the absenc...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-009-1549-z
更新日期：2009-10-01 00:00:00
abstract：:It was determined that there is a relationship between prevalence and mean densities of Trichodina spp. on Merlangius merlangus and organic pollution, measured as levels of nitrite, nitrate and phosphate in the surrounding environment. Monthly, two left anterior gill arcs of 60 M. merlangus (unless otherwise stated) c...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-005-1346-2
更新日期：2005-06-01 00:00:00
abstract：:Sarcocystis neurona is the parasite associated with equine protozoal myeloencephalitis (EPM). Recently, cats (Felis domesticus) have been implicated as a potential intermediate host in the life cycle of S. neurona. This study was initiated to determine whether cats have antibodies that react to S. neurona antigens sim...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s004360100503
更新日期：2002-01-01 00:00:00
abstract：:Allicin is an active ingredient of garlic that has antibacterial, antifungal, antiviral, and antiprotozoal activity. However, the inhibitory effects of allicin on Babesia parasites have not yet been examined. In the present study, allicin was tested as a potent inhibitor against the in vitro growth of bovine and equin...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-013-3654-2
更新日期：2014-01-01 00:00:00
abstract：:On the basis of information acquired from local health authorities in Evora district of Portugal on cases of visceral leishmaniasis (VL), an epidemiology survey study was conducted. To determine the prevalence of anti-Leishmania antibodies in the local human and canine populations residing in Evora town and 14 adjacen...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/BF00937115
更新日期：1995-01-01 00:00:00
