Abstract:
:Fecal specimens from 432 pre-weaned calves younger than 35 days were collected over a 2-year period (2010-2012) from 74 dairy cattle farms in the central area of Colombia. These samples were microscopically examined for the presence of Cryptosporidium oocysts, and positive specimens were selected for molecular examination. Microscopy revealed that 115 calves (26.6%) from 44 farms (59.5%) tested positive. Oocyst shedding was recorded in calves aged 3-day-old onwards, although the infection rate peaked at 8-14 days (40.7%). Infection rates were higher in diarrheic (52.2%) than in non-diarrheic calves (19.9%) (p < 0.0001, χ2), and infected calves had up to seven times more probability of having diarrhea than non-infected calves. Cryptosporidium species and subtypes were successfully identified in 73 samples from 32 farms. Restriction and sequence analyses of the SSU rRNA gene revealed C. parvum in all but two isolates identified as Cryptosporidium bovis. Sequence analyses of the 60-KDa glycoprotein (gp60) gene revealed eight subtypes within the IIa family. An unusual subtype (IIaA18G5R1) was the most prevalent and widely distributed (more than 66% specimens and 68% farms) while the subtype most frequently reported in cattle worldwide (IIaA15G2R1) was found in less than 13% of specimens and 16% farms. The remaining subtypes (IIaA16G2R1, IIaA17G4R1, IIaA20G5R1, IIaA19G6R1, IIaA20G6R1, and IIaA20G7R1) were restricted to 1-3 farms. This is the first large-sample size study of Cryptosporidium species and subtypes in Colombia and demonstrates the genetic uniqueness of this protozoan in cattle farms in this geographical area.
journal_name
Parasitol Resjournal_title
Parasitology researchauthors
Avendaño C,Ramo A,Vergara-Castiblanco C,Sánchez-Acedo C,Quílez Jdoi
10.1007/s00436-018-5818-6subject
Has Abstractpub_date
2018-05-01 00:00:00pages
1317-1323issue
5eissn
0932-0113issn
1432-1955pii
10.1007/s00436-018-5818-6journal_volume
117pub_type
杂志文章abstract::Lungworms of the genera Parafilaroides and Otostrongylus are responsible for parasitic bronchopneumonia, the foremost disease of eastern Atlantic common seals (EACS, Phoca vitulina vitulina) in the Dutch North Sea. Recently, there have been increased reports of lungworm cases and observations of unusually long Parafil...
journal_title:Parasitology research
pub_type: 杂志文章
doi:10.1007/s00436-020-06675-7
更新日期:2020-06-01 00:00:00
abstract::Myxobolus turpisrotundus Zhang, 2009, infects allogynogenetic gibel carp Carassius auratus gibelio (Bloch) and is always regarded as synonymous with Myxobolus rotundus Nemeczek, 1911, since its first report in goldfish Carassius auratus auratus (L.) in China in 1955. In this study, it was comprehensively examined by m...
journal_title:Parasitology research
pub_type: 杂志文章
doi:10.1007/s00436-010-1835-9
更新日期:2010-06-01 00:00:00
abstract::The var-gene encoding Plasmodium falciparum erythrocyte membrane protein 1 (PfEMP1) is known to play a major role in the pathogenicity of the P. falciparum parasite. The protein enables the parasite to adhere to the endothelial linings of small blood vessels (cytoadherence) as well as to non-infected erythrocytes (ros...
journal_title:Parasitology research
pub_type: 杂志文章
doi:10.1007/s00436-009-1583-x
更新日期:2009-11-01 00:00:00
abstract::Analysis of the initial interaction between Entamoeba histolytica trophozoites and the large intestine is impossible in humans and very difficult in experimental animals. To circumvent this obstacle we treated the luminal side of full-thickness rabbit colon segments mounted in Ussing-type chambers with trophozoite lys...
journal_title:Parasitology research
pub_type: 杂志文章
doi:10.1007/BF00931595
更新日期:1993-01-01 00:00:00
abstract::The nematode Anisakis simplex is a marine parasite that causes allergy as well as anisakiasis. Although five Anisakis allergens have already been identified, immunoblotting studies suggested that unidentified allergens still exist. In this study, an expression cDNA library constructed from A. simplex was subjected to ...
journal_title:Parasitology research
pub_type: 杂志文章
doi:10.1007/s00436-006-0396-4
更新日期:2007-05-01 00:00:00
abstract::Serine proteinases secreted by adult Trichinella spiralis were isolated from excretory/secretory products (ES) of in-vitro-cultured parasites by affinity chromatography with p-benzamidine-celite. The purified enzymes had molecular weights of approximately 18, 40, and 50 kDa and displayed enzyme activity against a rang...
journal_title:Parasitology research
pub_type: 杂志文章
doi:10.1007/pl00008552
更新日期:2000-08-01 00:00:00
abstract::The ultrastructure of the tegument, including putative subtegumental cells, of the monogenean gill parasite Macrogyrodactylus clarii is described for the first time. The lateral cells are secretory in nature and can be classified into four types: T1 producing elongated, electron-dense, bodies (t1); T2 manufacturing el...
journal_title:Parasitology research
pub_type: 杂志文章
doi:10.1007/s00436-006-0448-9
更新日期:2007-06-01 00:00:00
abstract::In April 2000, we collected and analyzed for parasites 174 feces belonging to 48 different and identified Cantabrian capercaillies (Tetrao urogallus cantabricus) from 39 lek sites in the Cantabrian Mountains (North Spain) and determined the factors related with prevalence and abundance of propagule excretion: (1) inte...
journal_title:Parasitology research
pub_type: 杂志文章
doi:10.1007/s00436-008-1015-3
更新日期:2008-08-01 00:00:00
abstract::Hookworm infection is still prevalent in southern Thailand despite control measures. Hookworm eggs submerged for an extended period under water from rainfall or in latrines may not survive, but they may recover their ability to develop into infective larvae when exposed to atmospheric air. This study examined the surv...
journal_title:Parasitology research
pub_type: 杂志文章
doi:10.1007/s00436-016-5031-4
更新日期:2016-07-01 00:00:00
abstract::Blastocystis is an emerging zoonotic pathogen with global distribution. However, limited data exist on the prevalence and genetic diversity of Blastocystis in the USA and in food animals. We conducted the first large-scale molecular investigation of Blastocystis in the USA by testing 2539 fecal samples from dairy heif...
journal_title:Parasitology research
pub_type: 杂志文章
doi:10.1007/s00436-018-6149-3
更新日期:2019-02-01 00:00:00
abstract::Spawning individuals of allis shad, Alosa alosa (Linnaeus, 1758), and twaite shad, Alosa fallax (Lacépède, 1803), were sampled from three rivers on the Atlantic coast of the Iberian Peninsula (Ulla, Minho, Mondego) during 2008 to 2013 to assess the presence of the zoonotic marine parasite Anisakis spp. larvae. The res...
journal_title:Parasitology research
pub_type: 杂志文章
doi:10.1007/s00436-015-4403-5
更新日期:2015-06-01 00:00:00
abstract::Levamisole phosphate, chosen based on its 100 % efficacy demonstrated by a previous fecal egg count reduction test (FECRT), was used as the exclusive anthelmintic treatment in the Embrapa Southeast Livestock sheep flock from 2009 to 2014 in a target selected treatment scheme. In the present study, the effectiveness of...
journal_title:Parasitology research
pub_type: 杂志文章
doi:10.1007/s00436-015-4844-x
更新日期:2016-03-01 00:00:00
abstract::Micronemes are characteristic secretory organelles located within the apical cell region of apicomplexan parasites. The protein contents are exocytosed during an early phase of host cell invasion and contribute to parasite motility and the invasion of target cells. We report here on the cloning and heterologous expres...
journal_title:Parasitology research
pub_type: 杂志文章
doi:10.1007/s00436-002-0600-0
更新日期:2003-05-01 00:00:00
abstract::The artemisinin-based combination therapy (ACT) is adopted by several countries as first line for malaria treatment in the last decade. Concomitantly, the World Health Organization and other research reports showed a dramatic decline in malaria burden in terms of morbidity, mortality and treatment failure (TF). The op...
journal_title:Parasitology research
pub_type: 杂志文章,评审
doi:10.1007/s00436-009-1700-x
更新日期:2010-02-01 00:00:00
abstract::Neurocysticercosis (NC) is one of the most important diseases caused by parasites affecting the central nervous system. We fractionated by ion-exchange chromatography using diethylaminoethyl (DEAE)-sepharose resin the total saline extract (S) from Taenia solium metacestodes and evaluated obtained fractions (DEAE S1 an...
journal_title:Parasitology research
pub_type: 杂志文章
doi:10.1007/s00436-014-3908-7
更新日期:2014-07-01 00:00:00
abstract::Excretory/secretory (ES) products collected from exsheathed L3 or parasitic stages of Haemonchus contortus were tested in vitro for gastrin stimulatory properties using an ovine abomasal antral mucosal preparation. In addition, the motility of exsheathed L3 and parasites recovered on weeks 2, 6 and 8 post-infection wa...
journal_title:Parasitology research
pub_type: 杂志文章
doi:10.1007/s00436-004-1274-6
更新日期:2005-01-01 00:00:00
abstract::The proboscis apex of the eoacanthocephalan parasite Paratenuisentis ambiguus was studied, using electron and light microscopy, for a better understanding of the parasite's attachment at the intestinal wall of its definitive hosts (Anguilla rostrata and A. anguilla). The results suggest the presence of an epidermis co...
journal_title:Parasitology research
pub_type: 杂志文章
doi:10.1007/pl00008583
更新日期:2001-04-01 00:00:00
abstract::Members of the Naegleria genus are free-living amoebae, and the only pathogenic specie described to date for humans is N. fowleri. However, as the complete genome of this specie has not been reported, non-pathogenic N. gruberi is employed to describe molecular pathways in N. fowleri. Regardless, certain mechanisms, su...
journal_title:Parasitology research
pub_type: 杂志文章
doi:10.1007/s00436-016-5293-x
更新日期:2017-01-01 00:00:00
abstract::Schistosomiasis is the second widespread tropical disease that affects the health of over 240 million people of 78 countries. Questionnaires have been commonly used to diagnose schistosomiasis, while no meta-analysis of their efficacy had been reported previously. This meta-analysis was conducted to assess their diagn...
journal_title:Parasitology research
pub_type: 杂志文章,meta分析
doi:10.1007/s00436-015-4579-8
更新日期:2015-09-01 00:00:00
abstract::The nervous system of Cysticercus cellulosae, the metacestode stage of the tapeworm Taenia solium, was delineated using histochemical methods for the localization of the enzyme markers; nonspecific esterase and acetylcholinesterase. The main features of the nervous system include a pair of cerebral ganglia, a circumce...
journal_title:Parasitology research
pub_type: 杂志文章
doi:10.1007/BF00936456
更新日期:1992-01-01 00:00:00
abstract::Field and experimental bovine infection sera were used in immunoblots of sporozoite and schizont lysates of Theileria parva to identify candidate diagnostic antigens. Four parasite antigens of Mr 67,000 (p67), 85,000 (the polymorphic immunodominant molecule, PIM), 104,000 (p104), and 150,000 (p150) were selected for a...
journal_title:Parasitology research
pub_type: 杂志文章
doi:10.1007/s004360050419
更新日期:1998-05-01 00:00:00
abstract::A parasitological survey of 651 northern fur seals Callorhinus ursinus L. from five subpopulations was conducted on St. Paul Island, Alaska, during July-August 2012-2014. Digenean trematodes were found in 210 of 651 fur seals with a total prevalence of 32.3%. Intensity of infection varied from 1 to 1540 parasites with...
journal_title:Parasitology research
pub_type: 杂志文章
doi:10.1007/s00436-018-5784-z
更新日期:2018-04-01 00:00:00
abstract::Strongyloides stercoralis is an intestinal nematode in humans, distributed through tropical and subtropical regions of the world. In most individuals, the infection has a chronic nature due to auto-infection at the low level. Accelerated auto-infection, mainly after an alteration in immune status, can cause a syndrome...
journal_title:Parasitology research
pub_type: 杂志文章
doi:10.1007/s00436-007-0531-x
更新日期:2007-08-01 00:00:00
abstract::In the present study, Diplectanum laubieri was redescribed from material obtained from wild and cultured fish. The population dynamics of D. aequans and D. laubieri under culture conditions was studied. The prevalence and mean intensity (average number of worms per parasitized fish) were 80.64% and 112 for D. aequans ...
journal_title:Parasitology research
pub_type: 杂志文章
doi:10.1007/BF00930906
更新日期:1991-01-01 00:00:00
abstract::Intrauterine embryonic development in the caryophyllidean tapeworm Khawia sinensis has been investigated using transmission electron microscopy and cytochemical staining with periodic acid-thiosemicarbazide-silver proteinate for glycogen. Contrary to previous light microscopy findings that reported the release of non-...
journal_title:Parasitology research
pub_type: 杂志文章
doi:10.1007/s00436-011-2590-2
更新日期:2012-02-01 00:00:00
abstract::Exposure to viable Acanthamoeba may cause fatal encephalitis and blinding keratitis in humans. Quantification of environmental Acanthamoeba by a reliable analytical assay is essential to assess the risk of human exposure and efficacy of control measures (e.g., superheating). Two DNA binding dyes (ethidium monoazide (E...
journal_title:Parasitology research
pub_type: 杂志文章
doi:10.1007/s00436-013-3556-3
更新日期:2013-11-01 00:00:00
abstract::Systematic, continuous mosquito surveillance is considered the most reliable tool to predict the spread and establishment of alien mosquito species such as the Asian tiger mosquito (Aedes albopictus), Japanese bush mosquito (Aedes japonicus), and the transmission risk of mosquito-borne arboviruses to humans. Only sing...
journal_title:Parasitology research
pub_type: 杂志文章
doi:10.1007/s00436-019-06287-w
更新日期:2019-05-01 00:00:00
abstract::Three juvenile nematode parasites were collected naturally from 90 (75 %) out of 120 specimens of the marine greater lizard fish Saurida undosquamis captured from water coasts at Hurghada City along the Red Sea in Egypt during the period from September 2013 to April 2014. Worms were identified on the basis of light an...
journal_title:Parasitology research
pub_type: 杂志文章
doi:10.1007/s00436-014-4285-y
更新日期:2015-03-01 00:00:00
abstract::Giardia lamblia and Cryptosporidium parvum are recognized as the most common protozoan infections in Saudi Arabia. Microscopic examination of stool samples, either direct or concentrated, for the recovery of G. lamblia cysts and trophozoites and C. parvum oocysts is still the most commonly used for the diagnosis of bo...
journal_title:Parasitology research
pub_type: 杂志文章
doi:10.1007/s00436-013-3319-1
更新日期:2013-04-01 00:00:00
abstract::Macroscopically visible sarcocysts were observed in the skeletal muscles of naturally infected skinks of the genus Agama (infection rate 11.3%). Sarcocysts were described by means of transmission electron microscopy. These cysts measured 0.03-0.25 x 0.38-1.7 mm (mean 0.12 x 1.1 mm). Typical mature cysts were bordered ...
journal_title:Parasitology research
pub_type: 杂志文章
doi:10.1007/pl00008559
更新日期:2000-09-01 00:00:00