Abstract:
:Micronemes are characteristic secretory organelles located within the apical cell region of apicomplexan parasites. The protein contents are exocytosed during an early phase of host cell invasion and contribute to parasite motility and the invasion of target cells. We report here on the cloning and heterologous expression of a novel member of the Sarcocystis muris microneme lectin family. The deduced amino acid sequence is in total agreement with that obtained after sequencing the native protein and is characterized by two copies of the apple domain motif. The recombinant polypeptide is expressed in a biologically active conformation as demonstrated by its galactose binding properties.
journal_name
Parasitol Resjournal_title
Parasitology researchauthors
Klein H,Mueller S,Loeschner B,Toenjes RR,Braun G,Mueller EC,Otto A,Montag Tdoi
10.1007/s00436-002-0600-0subject
Has Abstractpub_date
2003-05-01 00:00:00pages
84-6issue
1eissn
0932-0113issn
1432-1955journal_volume
90pub_type
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