Abstract：
:A pellet freezing method, one type of rapid freezing protocol, was established for Trichomonas vaginalis. As cryoprotectants, 5-20% dimethylsulfoxide (DMSO), ethylene glycol, propylene glycol, and glycerol were used. Of these, DMSO showed a high protective effect, with a maximum effect at a concentration of 20%. No effect of equilibration time was recognized with any of the cryoprotectants. Therefore, the optimal condition was deemed 20% DMSO without equilibration. The pellet freezing method was also compared with the conventional slow cooling protocol. The survival rate was 73 +/- 8% with the slow cooling protocol and 48 +/- 4% with the pellet freezing method. The delay in growth with the pellet freezing method was no more than 5.4 h compared with the slow cooling protocol, suggesting its validity as a technique for cryopreservation of T. vaginalis.
journal_name
Parasitol Resjournal_title
Parasitology researchauthors
Matsuo Jdoi
10.1007/s00436-007-0559-ysubject
Has Abstractpub_date
2007-09-01 00:00:00pages
907-11issue
4eissn
0932-0113issn
1432-1955journal_volume
101pub_type
杂志文章abstract：:Experiments were undertaken to develop a serum-free medium for the in vitro cultivation of Babesia caballi, a tick-borne hemoprotozoan parasite, one of the causative agents of equine piroplasmosis. A modified HL-1 medium supplemented with horse serum, L-glutamine, antibiotics, and hypoxanthine was used. B. caballi org...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s004360050568
更新日期：1999-05-01 00:00:00
abstract：:We analyzed extra chromosomal genomes of avian blood protozoa, Leucocytozoon caulleryi. One of the genomes, the mitochondrial genome was completely sequenced resulting 5,959 bp in length. This genome contained the identical gene organization and contents to that of other avian blood protozoa previously analyzed: three...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-008-1083-4
更新日期：2008-09-01 00:00:00
abstract：:Praziquantel is still very effective for the treatment of schistosomiasis, but there are rising concerns on the potential risk of developing resistances because of the extensive use of this drug. Triclabendazole, a systemic anthelmintic, is very effective against other trematodes such as Paragonimus spp. and Fasciola ...
journal_title：Parasitology research
pub_type： 临床试验,杂志文章
doi：10.1007/s00436-007-0802-6
更新日期：2008-03-01 00:00:00
abstract：:Echinostoma trivolvis adults grown in golden hamsters were treated in vitro with [3H]-tyrosine to label sperm and transplanted to uninfected hamsters alone or with four to six unlabeled adults. After 4-5 days, worms were recovered, processed for autoradiography, and observed for silver grains over their seminal recept...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/BF00932258
更新日期：1993-01-01 00:00:00
abstract：:Recent studies regarding the harmful effects of synthetic larvicides initiated the need to investigate for unconventional measures that are environmentally safe and target-specific against Aedes aegypti larvae. Thus, the main objectives of the study are to evaluate the larvicidal toxicity of the solvent fractions of A...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-015-4598-5
更新日期：2015-10-01 00:00:00
abstract：ABTRACT:Structure of the helminth community of Megaleporinus obtusidens collected in Lake Guaíba was evaluated, and the results indicated that the diversity of helminth species was probably determined by fish behavior and eating habits. The influence of sex, weight, and standard length of hosts for parasitic indices wa...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-018-5933-4
更新日期：2018-08-01 00:00:00
abstract：:We examined the in vitro effect of increasing gliotoxin concentrations on the infectivity of Toxoplasma gondii for NIH-3T3 murine fibroblasts and on Toxoplasma chymotrypsin-like activity, which is specific to the proteasome. Parasite penetration of host cells was not modified by a high gliotoxin concentration (1 micro...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-002-0644-1
更新日期：2002-08-01 00:00:00
abstract：:Feline lungworms infect the respiratory tract of wild and domestic cats, causing infection often associated with clinical disease. Until recently, Aelurostrongylus abstrusus has been considered the most relevant species of lungworm, while Troglostrongylus brevior was considered of less significance. Fecal samples of f...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-020-06852-8
更新日期：2020-10-01 00:00:00
abstract：:Haemonchus contortus isolates were evaluated for benzimidazole (BZ) resistance or susceptibility by allele-specific PCR based on β-tubulin isotype 1 gene polymorphisms at the F167Y, E198A, and F200Y sites. Two isolates, one presumed susceptible from wild pronghorn antelope (PH) and one known to be resistant from goats...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-016-5357-y
更新日期：2017-02-01 00:00:00
abstract：:This paper reports results of the first cytogenetic study carried out on a recently described monozoic tapeworm, Khawia saurogobii Xi et al., 2009, from the Chinese lizard gudgeon (Saurogobio dabryi). The karyotype of this species is composed of eight pairs of metacentric and telocentric chromosomes (2n = 16; n = 3m +...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-009-1702-8
更新日期：2010-02-01 00:00:00
abstract：:Serine oligopeptidases of trypanosomatids are emerging as important virulence factors and therapeutic targets in trypanosome infections. A complete open reading frame of oligopeptidase B from Leishmania amazonensis was amplified with polymerase chain reaction with gradient annealing temperatures using primers designed...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-007-0552-5
更新日期：2007-09-01 00:00:00
abstract：:Diversity in parasite virulence is one of the factors that contribute to the clinical outcome of malaria infections. The association between the severity of Plasmodium falciparum malaria and the number of distinct parasite populations infecting the host (multiplicity of infection) or polymorphism within any of the spe...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-013-3325-3
更新日期：2013-04-01 00:00:00
abstract：:Ticks spend most of their life cycle in the environment, and all tick life cycle stages are dependent on a complex combination of climate variables. Furthermore, host availability and vegetation significantly modulate the dynamics of tick populations. Tick recruitment is dependent on successful reproduction, which in ...
journal_title：Parasitology research
pub_type： 杂志文章,评审
doi：10.1007/s00436-008-1056-7
更新日期：2008-12-01 00:00:00
abstract：:Exsheathment and midgut invasion of nocturnally subperiodic Brugia malayi microfilariae were analyzed using light and scanning electron microscopy in a refractory vector, Aedes aegypti (Thailand strain). Results showed that exsheathed microfilariae represented only approximately 1% of the total microfilaria midguts di...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-014-4086-3
更新日期：2014-11-01 00:00:00
abstract：:Mixed infections with Schistosoma japonicum and S. mansoni were carried out in mice. S. japonicum females paired with S. mansoni males developed normally and produced numerous viable eggs; very little sperm was found in the female genital tract. The eggs yielded many miracidia infective to Oncomelania hupensis, the ho...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/BF00932672
更新日期：1994-01-01 00:00:00
abstract：:A molecular phylogenetic hypothesis is presented for the genus Trichuris based on sequence data from mitochondrial cytochrome c oxidase 1 (cox1) and cytochrome b (cob). The taxa consisted of nine populations of whipworm from five species of Sigmodontinae rodents from Argentina. Bayesian Inference, Maximum Parsimony, a...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-016-5045-y
更新日期：2016-08-01 00:00:00
abstract：:Serum and colostrum were collected from adult buffalo cows naturally infected with Toxocara vitulorum. When injected into mice, the colostrum reduced the number of larvae of T. vitulorum that migrated in the mice. Injection of particularly the IgG-containing fraction but also the IgM-containing fraction of Sephadex G2...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/BF00932381
更新日期：1994-01-01 00:00:00
abstract：:Serine proteinases secreted by adult Trichinella spiralis were isolated from excretory/secretory products (ES) of in-vitro-cultured parasites by affinity chromatography with p-benzamidine-celite. The purified enzymes had molecular weights of approximately 18, 40, and 50 kDa and displayed enzyme activity against a rang...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/pl00008552
更新日期：2000-08-01 00:00:00
abstract：:A retrospective study was carried out on the experimental infections of Lymnaea truncatula with Fasciola hepatica performed over the last 20 years to determine if the populations of snails living in highland or lowland countries had the same ability to sustain trematode larval development. The six highland populations...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-001-0542-y
更新日期：2002-04-01 00:00:00
abstract：:This study aims to observe the histopathological alterations in schistosomula of Schistosoma japonicum induced by mefloquine. Mice were infected with S. japonicum cercariae, and after 14 days, a single dose of mefloquine (400 mg/kg) was administered orally. After 8 h, 24 h, 3 days, 7 days, and 14 days, groups of two t...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-009-1611-x
更新日期：2009-11-01 00:00:00
abstract：:The problems of parasite resistance, as well as the toxic residues to most of the commercially available antipiroplasmic drugs severely weaken their effective, curative, and environmental safe employment. Therefore, it is clear that the development of treatment options for piroplasmosis is vital for improving disease ...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-020-06796-z
更新日期：2020-09-01 00:00:00
abstract：:Spermiogenesis and the ultrastructure of the spermatozoon of the bothriocephalidean cestode Clestobothrium crassiceps (Rudolphi, 1819), a parasite of the teleost fish Merluccius merluccius (Linnaeus, 1758), have been studied by means of transmission electron microscopy. Spermiogenesis involves firstly the formation of...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-011-2446-9
更新日期：2012-01-01 00:00:00
abstract：:Sheep infected with the triclabendazole-susceptible Cullompton isolate of Fasciola hepatica were dosed with 15 mg/kg of compound alpha at 12 weeks post-infection. Adult flukes were recovered from the bile ducts at 24, 48, and 72 h post-treatment (p.t.). Changes to the spermatogenic cells in the testis were examined by...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-009-1649-9
更新日期：2010-01-01 00:00:00
abstract：:In the present study the behavior and pathogenicity of second-stage larvae of Toxocara canis were examined in different mouse strains with special emphasis on the major histocompatibility complex (MHC). Mice of the inbred strains BALB, C3H, C57BL, and DBA and the outbred strain NMRI were infected orally with 1000 seco...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/BF00932955
更新日期：1994-01-01 00:00:00
abstract：:A study has been carried out to investigate whether the action of triclabendazole (TCBZ) against Fasciola hepatica is altered by inhibition of drug metabolism. The cytochrome P450 (CYP 450) enzyme pathway was inhibited using ketoconazole (KTZ) to see whether a TCBZ-resistant isolate could be made more sensitive to TCB...
journal_title：Parasitology research
pub_type： 更正并重新发布的文章,杂志文章
doi：10.1007/s00436-011-2554-6
更新日期：2011-10-01 00:00:00
abstract：:The principal health problem in small ruminants is helminthiasis and the rapid development of nematode resistance to anthelminthics has limited the success of control in several countries, stimulating the search for alternatives. In this study, extracts of immature fruits of the mango Mangifera indica L. var Ubá were ...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-012-3017-4
更新日期：2012-12-01 00:00:00
abstract：:Clonorchiasis, caused by Clonorchis sinensis infection, is a zoonotic parasitic disease of hepatobiliary system in which the proteins released by adult are major pathogenetic factors. In this study, we first characterized a putative sphingomyelin phosphodiesterase (CsSMPase) A-like secretory protein, which was highly ...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-013-3388-1
更新日期：2013-06-01 00:00:00
abstract：:Monosaccharides, lectins, periodate, trypsin and neuraminidase were used to analyse the process of adhesion of Giardia duodenalis trophozoites to IEC cells, an intestinal epithelial cell line. Addition of N-acetyl-glucosamine, N-acetyl-galactosamine, galactose and fucose to the interaction medium inhibited attachment ...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/BF02351874
更新日期：1994-01-01 00:00:00
abstract：:In this study, the larvicidal activity of an enriched fraction of the major lipophilic phenolic compounds from Hypericum carinatum Griseb. (Clusiaceae) was investigated against larvae of Aedes aegypti (Diptera: Culicidae), the main vector of dengue virus in Brazil. The larval mortality rate ranged 37.33 to 72.00 % at ...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-013-3401-8
更新日期：2013-06-01 00:00:00
abstract：:The third larval stage (L3) of Anisakis simplex (Anisakidae) is one of the zoonotic parasitic nematodes in the musculature and visceral organs of marine fishes belonging to family Moronidae. The consumption of these high-commercial-value fish is widespread in many countries around the Mediterranean Sea including Egypt...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-015-4500-5
更新日期：2015-08-01 00:00:00
