Abstract：
:The purified metalloprotease and the partially purified cysteine protease from pathogenic Cryptobia salmositica were characterized. Using haemoglobin gel electrophoresis, we detected five enzymatic bands in crude parasite lysate; one protease (200 kDa) yielded a metalloprotease band and other four, cysteine protease bands (97, 70, 66 and 49 kDa). Both the metalloprotease and the cysteine protease had high levels of proteolytic activity against azocasein, haemoglobin and fibrinogen. The metalloprotease had high levels of activity against azocoll and gelatin but a low degree of activity against albumin. In contrast, the cysteine protease had extensive activity against albumin but low levels of activity against azocoll and gelatin. The metallo- and cysteine proteases had no activity against Pz-peptide, a specific substrate for bacterial collagenase. The optimal pH for the metalloprotease and the cysteine protease was 7.0 and 5.0, respectively. The metalloprotease was inhibited by metal-chelating agents and excess of zinc ions but was activated by calcium ions. The cysteine protease was inhibited by thiol-blocking agents. The natural antiprotease alpha2-macroglobulin, but not alpha1-protease inhibitor, inhibited the activity of both proteases from C. salmositica. The optimal in vitro temperature for the purified metalloprotease was 30 degrees C.
journal_name
Parasitol Resjournal_title
Parasitology researchauthors
Zuo X,Woo PTdoi
10.1007/s004360050435subject
Has Abstractpub_date
1998-06-01 00:00:00pages
492-8issue
6eissn
0932-0113issn
1432-1955journal_volume
84pub_type
杂志文章abstract：:Climate change with an increase in average temperature may be responsible for propagation of temperature-dependent vectors and/or vector-associated pathogens in regions that were previously not endemic. The analysis of climate data of Brandenburg state (Germany) had showed that the extrinsic development of Dirofilaria...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-013-3431-2
更新日期：2013-07-01 00:00:00
abstract：:The value profiles of 5 intracellular enzymes, 15 metabolites (with 2 associated ratios), and 3 electrolytes were monitored over time in 9 captive-reared African black-footed penguins (Spheniscus demersus) with different avian malaria clinical status: uninfected, subclinically infected, and clinically infected with fa...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/BF00931537
更新日期：1995-01-01 00:00:00
abstract：:Morphology of capsules surrounding acanthocephalan Corynosoma strumosum in paratenic hosts (sea fishes of three species from the northern part of the Sea of Okhotsk) was studied. A thick layer of glycocalyx is formed on the surface of acanthocephalan's tegument in smelts Osmerus mordax dentex and Hypomesus olidus; the...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-010-2088-3
更新日期：2011-02-01 00:00:00
abstract：:Tryptophan-N-formylated gramicidin A, a nonhemolytic derivative of the toxic peptide antibiotic gramicidin A, has previously been shown to induce potassium leakage from Plasmodium falciparum-infected erythrocytes in vitro and to inhibit the growth of the parasite. In the present study the antimalarial activities of tw...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/BF00932413
更新日期：1995-01-01 00:00:00
abstract：:Ultrastructural changes of gastritis due to infection with Physaloptera clausa in 12 fresh carcasses of euthanized European hedgehogs (Erinaceus europaeus) collected from different part of Urmia, Iran, in which they were highly populated with this animal, six females and six males were subjected to detail necropsy wit...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-015-4597-6
更新日期：2015-10-01 00:00:00
abstract：:The invasive fish tapeworm, Khawia japonensis (Yamaguti, 1934) originally described in Japan, is reported for the first time in Slovakia. The tapeworm was found in farmed common carp (Cyprinus carpio L.) from East-Slovakian breeding fish pond. This finding is registered 4 years after the first announcement of this par...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-015-4447-6
更新日期：2015-05-01 00:00:00
abstract：:Spermatogenesis, testes, and vas deferens in the parthenogenetic monozoic tapeworm Atractolytocestus huronensis Anthony, 1958 (Cestoda: Caryophyllidea) from Slovakia, parasitizing the carp Cyprinus carpio L., have been investigated by means of transmission electron microscopy for the first time. The present results sh...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-010-2038-0
更新日期：2011-01-01 00:00:00
abstract：:This study assessed the anisakid nematode distribution pattern in the fish collected from coasts of Mediterranean Sea, Egypt, during the period September 2010-April 2011. Two hundred thirty out of 300 (76.7%) Dicentrarchus labrax (European seabass) marine fishes belonging to family Moronidae were dissected and found t...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-011-2600-4
更新日期：2012-03-01 00:00:00
abstract：:Intestinal coccidiosis of rabbits induced by E. coecicola causes enormous economic losses in rabbit farms. Here, we investigate the effect of E. coecicola on the liver of the rabbit Oryctolagus cuniculus. On day 7 p.i., fecal expulsion of E. coecicola oocysts is maximal and rabbits have lost approximately 25% of their...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-011-2574-2
更新日期：2012-02-01 00:00:00
abstract：:Little is known of the prevalence and genetic identity of Giardia duodenalis in sheep in Algeria. The present study aimed at characterizing G. duodenalis in lambs up to 6 months of age in Djelfa, Algeria. A total of 346 fecal specimens were collected from 28 farms and screened for G. duodenalis cysts by zinc sulfate f...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-020-06808-y
更新日期：2020-09-01 00:00:00
abstract：:Although cystic echinococcosis (CE) has been a recognized public health problem in Greece, molecular data are lacking regarding the types and prevalences of infecting strains of the etiological agent Echinococcus granulosus. Therefore, we investigated the prevalence of CE and determined the infecting genotypes in shee...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-007-0568-x
更新日期：2007-09-01 00:00:00
abstract：:The present study uses scanning and transmission electron microscopical observations to examine the haptoral features of the hexabothriid polyopisthocotylean monogenean Rajonchocotyle emarginata from the gills of the elasmobranch Amblyraja radiata. The haptor possesses six equal, pedunculate suckers, each armed with a...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-016-4973-x
更新日期：2016-06-01 00:00:00
abstract：:Changes in the hepatocytes of male hamsters (Mesocricetus auratus) and jirds (Meriones unguiculatus) at 220 days after experimental infection with the liver fluke Opisthorchis viverrini were studied by light and electron microscopy. The hepatocytes of the control group were characterized by an intracellular compartmen...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/BF00931823
更新日期：1993-01-01 00:00:00
abstract：:Trichomonas vaginalis, a flagellated protozoan parasite causes a variety of adverse health consequences in both men and women. The parasite exists in the trophozoite and the pseudocystic stage. The study reports for the first time that pseudocyst forms of T. vaginalis isolated from cervical neoplasia (CN) patients dem...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-012-2848-3
更新日期：2012-07-01 00:00:00
abstract：:Dermanyssus gallinae (Acari: Mesostigmata) is the most important hematophagous ectoparasite in layer farms in many countries. The reproduction rate of the parasite is rapid and can be completed in a week under favorable conditions. The parasite has direct and indirect effects on birds. It can also act as a vector for ...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-014-3759-2
更新日期：2014-03-01 00:00:00
abstract：:The inhibitory activity of mangiferin (50 mg/kg/die and 100 mg/kg/die) on Cryptosporidium parvum was evaluated in a neonatal mouse model and its activity was compared with that of paromomycin (100 mg/kg/die). At 4 days of age, neonatal Swiss conventional outbred mice were experimentally infected by oral administration...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-006-0165-4
更新日期：2006-07-01 00:00:00
abstract：:Ticks can significantly affect the health and fitness of the host. Seasonal population dynamics of ticks play a vital role in disease transmission and the shaping of life-history traits of both tick and host. In this study, we examine the seasonal population dynamics of Ixodes hirsti in South Australia. For 2 years, w...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-008-1071-8
更新日期：2008-09-01 00:00:00
abstract：:Parasites of managed bees can disrupt the colony success of the host, but also influence local bee-parasite dynamics, which is regarded as a threat for wild bees. Therapeutic measures have been suggested to improve the health of managed bees, for instance, exploiting the bees' RNA interference (RNAi) pathway to treat ...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-018-6176-0
更新日期：2019-02-01 00:00:00
abstract：:Host susceptibility to parasites is likely to be influenced by intrinsic factors, such as host oxidative status determined by the balance between pro-oxidant production and antioxidant defences. As a result, host oxidative status acts as an environmental factor for parasites and may constrain parasite development. We ...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-018-5869-8
更新日期：2018-07-01 00:00:00
abstract：:The present study deals with investigation of larvicidal activity and their chemical constituents of the essential oil from the seeds of Nigella sativa L. (Ranunculaceae). Totally, 18 chemical compounds were identified by GC and GC-MS analysis. Thymol (19.13 %) and α-phellandrene (14.9 %) were identified as major chem...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-015-4563-3
更新日期：2015-09-01 00:00:00
abstract：:For years, mammals of the order Pilosa have been considered Leishmania reservoirs. But while most studies have focused on sloth species, anteaters have been overlooked, and in many Leishmania endemic countries like Mexico, no studies have been carried out. The aims of this work were to identify the presence of Leishma...
journal_title：Parasitology research
pub_type： 杂志文章,评审
doi：10.1007/s00436-019-06253-6
更新日期：2019-04-01 00:00:00
abstract：:Parasite-mediated selection is currently believed to play an important role in life-history evolution. To assess how simple hematoserological and biochemical condition indices reflect host immunocompetence and infection resistance controlled laboratory experiments are required. We addressed these issues by infecting l...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-006-0142-y
更新日期：2006-04-01 00:00:00
abstract：:The viability of Cryptosporidium parvum oocysts ingested by Artemia franciscana metanauplii was evaluated using two fluorogenic vital dyes. There was no significant difference (p = 0.09) between the viability of oocysts maintained in saline (control) and those recovered from the digestive tract of the microcrustacean ...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-007-0543-6
更新日期：2007-08-01 00:00:00
abstract：:The uptake of amino acids is mediated by active transporters located on the basolateral and brush border membranes of intestinal epithelial cells. The current study investigated the expression of amino acid transporters (AAT) and other genes in the intestine of chicks infected with Eimeria maxima. At 7-day postinfecti...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-014-4114-3
更新日期：2014-10-01 00:00:00
abstract：:From March to October 2003, a total of 2,518 host-seeking Ixodes ricinus ticks (1,944 nymphs, 264 females, 310 males) were collected by blanket dragging at 45 sites all over the city area of Bonn, western Germany, to be checked for Borrelia burgdorferi infection. The collection sites included 20 private gardens, nine ...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-004-1240-3
更新日期：2005-01-01 00:00:00
abstract：:This study was conducted to identify genetic characteristics of Besnoitia spp. isolated from goat in Iran. Molecular analysis of two portions of nuclear ribosomal DNA (ITS1 and ITS2) was used for the genetic characterization of the Besnoitia species. Comparison of the sequencing data of the Iranian Besnoitia samples o...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-010-2107-4
更新日期：2011-03-01 00:00:00
abstract：:Infection with helminth parasites or the administration of their antigens can prevent or attenuate autoimmune diseases. To date, the specific molecules that prime the amelioration are only limited. In this study, recombinant Schistosoma japonicum cystatin (rSjcystatin) and fructose-1,6-bisphosphate aldolase (rSjFBPA) ...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-019-06511-7
更新日期：2020-01-01 00:00:00
abstract：:Black bullhead Ameiurus melas (Actinopterygii: Siluriformes) is an alien fish species of North American origin, which has expanded its invasive geographical range in Europe. In 2017-2019, 32 black bullhead specimens from the Lake Srebarna Biosphere Reserve, Bulgaria, were examined for monogenean parasites. Two species...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-020-06699-z
更新日期：2020-07-01 00:00:00
abstract：:Domestic cats and other felids are definitive hosts for the zoonotic protozoan parasite Toxoplasma gondii. Serology is widely used in epidemiological studies conducted to estimate the proportion of domestic cats that have encountered the parasite. However, a limited number of such studies are available from some regio...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-019-06368-w
更新日期：2019-08-01 00:00:00
abstract：:The oncomiracidium (larva) of the entobdelline Neoentobdella parvitesticulata (Monogenea: Capsalidae), adults of which parasitise the skin of the stingray Himantura fai (Dasyatidae) at Heron Island, Great Barrier Reef, Queensland, Australia, is described. The larva undergoes precocious development (progenesis) in the ...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-005-1399-2
更新日期：2005-07-01 00:00:00
