Molecular identification and phylogenetic analysis of Heterakis gallinae from native chickens in Egypt.

Abstract:

:The genomic DNA was extracted from nematode parasites, Heterakis gallinae from specific host, native chickens. The polymerase chain reaction (PCR) was applied to make amplification of the DNA content that aids in the further studies. The primer was used in the PCR reactions that have the following sequences: forward (5'-GTTTCCGTAGGTGAACCTGC-3') and reverse (5'-ATATGCTTAAGTTCAGCGGGT-3'). It gave total amplified fragment markers at 914 bp. The gene sequences obtained from Egyptian H. gallinae was to make phylogenetic relationships of Egyptian H. gallinae with other nematodes on the database of an accession number (AJ876757), showing that it was nearly similar to Heterakis gallinarum.

journal_name

Parasitol Res

journal_title

Parasitology research

authors

Bazh EK

doi

10.1007/s00436-013-3539-4

subject

Has Abstract

pub_date

2013-10-01 00:00:00

pages

3557-60

issue

10

eissn

0932-0113

issn

1432-1955

journal_volume

112

pub_type

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