Abstract：
:A quantitative colorimetric assay using the oxidation-reduction indicator resazurin was developed to measure cytotoxicity of compounds against the protozoan parasite Trypanosoma cruzi. This method is based on the detection of colorimetric changes caused by the oxidation (blue) and reduction (pink) capabilities of resazurin dye, an indicator for metabolic cell function. To validate the assay, the experimental conditions were adjusted, such as number of parasites, dye concentration, and time of incubation, with respect to linearity and lower limit of detection. We found that absorbances increased linearly, with the plating density of parasites as low as 5-100 x 10(4)/well (r=0.99; p<0.001) when they were incubated for 5 h at 28 degrees C in the presence of 10% resazurin solution (3 mM). When the cytotoxicity of the reference drugs nifurtimox and benznidazole was measured with this assay and compared to the microscopic counting method, the same range was obtained, demonstrating that the resazurin microtiter assay is valid for the screening of new trypanocidal compounds. This test is very simple, fast, sensitive, and cheap.
journal_name
Parasitol Resjournal_title
Parasitology researchauthors
Rolón M,Vega C,Escario JA,Gómez-Barrio Adoi
10.1007/s00436-006-0126-ysubject
Has Abstractpub_date
2006-07-01 00:00:00pages
103-7issue
2eissn
0932-0113issn
1432-1955journal_volume
99pub_type
杂志文章abstract：:Synoptic data and an understanding of helminth parasite diversity among diverse rodent assemblages across temperate latitudes of North America remain remarkably incomplete. Renewed attention to comprehensive survey and inventory to establish the structure of biodiverse faunas is essential in providing indicators and p...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-016-5255-3
更新日期：2016-12-01 00:00:00
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journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-019-06233-w
更新日期：2019-03-01 00:00:00
abstract：:This study assessed the anisakid nematode distribution pattern in the fish collected from coasts of Mediterranean Sea, Egypt, during the period September 2010-April 2011. Two hundred thirty out of 300 (76.7%) Dicentrarchus labrax (European seabass) marine fishes belonging to family Moronidae were dissected and found t...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-011-2600-4
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pub_type： 杂志文章
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更新日期：2010-10-01 00:00:00
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journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-012-3262-6
更新日期：2013-05-01 00:00:00
abstract：:Cryptosporidium is a significant pathogen in humans and animals. Cases of infection by C. meleagridis or C. baileyi with zoonotic potential have also been reported in domestic birds; and recent studies indicate the presence of new host-adapted species or genotype in birds. Therefore, accurately identifying isolates is...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-004-1082-z
更新日期：2004-04-01 00:00:00
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journal_title：Parasitology research
pub_type： 杂志文章
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更新日期：2008-08-01 00:00:00
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journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-017-5549-0
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journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-006-0428-0
更新日期：2007-06-01 00:00:00
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journal_title：Parasitology research
pub_type： 杂志文章,评审
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更新日期：2016-04-01 00:00:00
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journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-008-1275-y
更新日期：2009-04-01 00:00:00
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journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s004360050497
更新日期：1998-10-01 00:00:00
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journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-010-1932-9
更新日期：2010-09-01 00:00:00
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journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-017-5709-2
更新日期：2018-01-01 00:00:00
abstract：:Many malaria-endemic areas are also associated with high rates of human immunodeficiency virus (HIV) infection. An understanding of the chemotherapeutic interactions that occur during malaria and HIV co-infections is important. Our previous studies have demonstrated that some antiretroviral protease inhibitors are eff...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-011-2427-z
更新日期：2011-12-01 00:00:00
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journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-009-1412-2
更新日期：2009-08-01 00:00:00
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journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-009-1512-z
更新日期：2009-10-01 00:00:00
abstract：:Knowledge of species and genotypes on Cryptosporidium involved in human transmission is fundamental in studying the epidemiology of cryptosporidiosis. This is the first study in molecular epidemiology of cryptosporidiosis that has been made in Chile. Cryptosporidium parvum species and genotypes involved in human and b...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-005-1391-x
更新日期：2005-08-01 00:00:00
abstract：:Seventy-five previously frozen Northern Lapwings, Vanellus vanellus (Linnaeus, 1758) were dissected and examined for trematodes. The available birds were euthanized after suffering severe injuries from a hail storm. Trematode specimens were found in the air sacs, body cavities and kidneys. They were cleared, stained i...
journal_title：Parasitology research
pub_type： 杂志文章
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更新日期：2014-02-01 00:00:00
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journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-015-4456-5
更新日期：2015-07-01 00:00:00
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journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-005-1301-2
更新日期：2005-03-01 00:00:00
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pub_type： 杂志文章
doi：10.1007/BF00531638
更新日期：1988-01-01 00:00:00
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journal_title：Parasitology research
pub_type： 杂志文章,评审
doi：10.1007/s00436-007-0782-6
更新日期：2008-02-01 00:00:00
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journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-007-0684-7
更新日期：2007-09-01 00:00:00
abstract：:This analysis investigated the influence of breed and gender on the pharmacokinetics of monepantel, and influence of breed, age, and gender on its efficacy against gastrointestinal nematodes of sheep. In a comparison of pharmacokinetic profiles from two studies, Merino lambs had significantly greater maximum concentra...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-009-1671-y
更新日期：2010-01-01 00:00:00
abstract：:Discoid granules were isolated from the tegument of adult Schistosoma mansoni by differential centrifugation of freeze-thaw extracts. Comparison of proteins and glycoproteins from discoid granules, surface membranes and the soluble fraction implied that discoid granules and the surface membrane were functionally linke...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/BF00539573
更新日期：1988-01-01 00:00:00
abstract：:This study was made to investigate efficacy of eprinomectin against to Toxocara canis in dogs. In the study, 20 stray dogs naturally infected with T. canis were divided into two groups as treatment (ten dogs) and control (ten dogs). Eprinomectin (100 microg/kg, Eprinex 250 ml) was given to treatment group dogs orally,...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-007-0776-4
更新日期：2008-02-01 00:00:00
abstract：:Schistosomiasis is the second widespread tropical disease that affects the health of over 240 million people of 78 countries. Questionnaires have been commonly used to diagnose schistosomiasis, while no meta-analysis of their efficacy had been reported previously. This meta-analysis was conducted to assess their diagn...
journal_title：Parasitology research
pub_type： 杂志文章,meta分析
doi：10.1007/s00436-015-4579-8
更新日期：2015-09-01 00:00:00
