Abstract：
:The purpose of this study was to evaluate the practicality of using real-time PCR for quantifying feces-derived trichostrongyle eggs. Haemonchus contortus eggs were used to evaluate fecal contaminants, time after egg embryonation, and the presence of competing and non-competing DNAs as factors that might interfere with generating reproducible results during simplex and multiplex quantitative real-time PCR (QPCR). Real-time PCR results showed linear quantifiable amplification with DNA from five to 75 eggs. However, threshold cycle (C (T)) values obtained by amplification of DNA from egg numbers between 75 and 1,000 did not differ significantly. Inhibitors of QPCR were effectively removed during DNA extraction as exemplified by the absence of any improvement in C (T) values with bovine serum albumin or phytase treatments. Changes from egg embryonation could only be detected during the first 6 h. Noncompetitive DNA did not appear to impact amplification; however, in a multiplex reaction a competing trichostrongyle such as Cooperia oncophora can hinder amplification of H. contortus DNA, when present at tenfold greater amounts. This study demonstrates the usefulness of QPCR for amplification and quantification of trichostrongyle eggs, and identifies potential limitations, which may be addressed through multiplex assays or the addition of a standard: exogenous DNA target.
journal_name
Parasitol Resjournal_title
Parasitology researchauthors
Harmon AF,Williams ZB,Zarlenga DS,Hildreth MBdoi
10.1007/s00436-006-0428-0subject
Has Abstractpub_date
2007-06-01 00:00:00pages
71-6issue
1eissn
0932-0113issn
1432-1955journal_volume
101pub_type
杂志文章abstract：:Clinostomum complanatum is an important trematode in fishes, birds of the family Ardeidae, and humans. Until now, limited knowledge is available regarding its molecular epidemiology, ecology, and phylogenetic study. Knowledge of the full mitochondrial genome of C. complanatum will provide important information for the...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-016-5102-6
更新日期：2016-08-01 00:00:00
abstract：:In an experiment including 8 groups of 15 mice, the effect of migrating Ascaris suum larvae in the lungs on the establishment and pathogenicity of aerosol exposure to Pasteurella multocida was investigated. Following aerosol exposure to P. multocida, mice with migrating A. suum in their lungs developed more severe pne...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/BF00931575
更新日期：1992-01-01 00:00:00
abstract：:Domestic cats and other felids are definitive hosts for the zoonotic protozoan parasite Toxoplasma gondii. Serology is widely used in epidemiological studies conducted to estimate the proportion of domestic cats that have encountered the parasite. However, a limited number of such studies are available from some regio...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-019-06368-w
更新日期：2019-08-01 00:00:00
abstract：:The CSP, EMP2/MESA, MSP2, MSP3, MSP5, RAP1, RAP2, RESA1, SERA1 and SSP2/TRAP genes of Plasmodium falciparum are vaccine candidates. The hepatic phase of the infection is of major interest due to the protection induced by immunization with radiation-attenuated sporozoites. We therefore performed RT-PCR experiments to d...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-003-1061-9
更新日期：2004-04-01 00:00:00
abstract：:The purified metalloprotease and the partially purified cysteine protease from pathogenic Cryptobia salmositica were characterized. Using haemoglobin gel electrophoresis, we detected five enzymatic bands in crude parasite lysate; one protease (200 kDa) yielded a metalloprotease band and other four, cysteine protease b...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s004360050435
更新日期：1998-06-01 00:00:00
abstract：:This study presents the ultrastructural characteristics of sporogenesis in the genus Myxobolus which infects four economically important Egyptian fishes: Oreochromis niloticus niloticus, Barbus bynni, Labeo niloticus and Hydrocynus forskalii, specimens of which were collected at Giza city. Although the cysts were obse...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-004-1250-1
更新日期：2005-02-01 00:00:00
abstract：:One cDNA clone was purified from an adult Clonorchis sinensis cDNA library, and its deduced polypeptide sequence was found to be homologous with myosin regulatory light chain (MRLC) of invertebrates and vertebrates. Two amino-acid residues, Thr and Ser, were conserved at the phosphorylation sites that regulate the fun...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-005-1376-9
更新日期：2005-08-01 00:00:00
abstract：:The uterine organization in Tetrabothrius erostris (Tetrabothriidea) was investigated by the methods of transmission and scanning electron microscopy. In sexually mature proglottids, the uterine wall consists of a syncytial epithelium (1.4-2.5 μm thick, except in regions containing nuclei). The ribosomes, mitochondria...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-014-4153-9
更新日期：2014-12-01 00:00:00
abstract：:Cyclospora spp. in nonhuman primates are most closely related to Cyclospora cayetanensis, an emerging human pathogen causing outbreaks of cyclosporiasis in North America. Studies thus far indicate the possible existence of host specificity in Cyclospora spp. In this study, 411 fecal specimens from free-range rhesus mo...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-015-4367-5
更新日期：2015-05-01 00:00:00
abstract：:Preliminary findings of a high prevalence of Clonorchis sinensis in wild-caught fish in a North Vietnam reservoir (Thac Ba reservoir, Yen Bai Province) prompted a longitudinal epidemiological study of fish infections. Monthly collections of fish from September 2014 to August 2015 were processed for recovery of metacer...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-016-5100-8
更新日期：2016-09-01 00:00:00
abstract：:Two of the three vector species of Schistosoma mansoni Sambon, 1907 in Brazil occur in the state of Maranhão: Biomphalaria glabrata (Say, 1818) and Biomphalaria straminea (Dunker, 1848). For the implementation of effective measures to combat schistosomiasis, it is necessary to identify the spatial and seasonal dynamic...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-018-5810-1
更新日期：2018-05-01 00:00:00
abstract：:A detailed study of the surface topography of the echinostome digenean Mesorchis denticulatus (Rudolphi 1802) Dietz 1909 was carried out using the scanning electron microscope. The surface area of the redia is increased by microvilli, and ciliate sensory structures are common around the mouth. The cannibalistic behavi...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/BF00536336
更新日期：1987-01-01 00:00:00
abstract：:Trichomonas gallinae are parasitic flagellates of importance in wild and domestic birds. The parasite is worldwide distributed, and Columbine birds are its main host. Current research focuses mostly on epidemiological and phylogenetic studies. However, there is still a lack of knowledge regarding parasite-host interac...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-020-06887-x
更新日期：2020-11-01 00:00:00
abstract：:The levels of parasite-specific IgG1 and IgG2 antibodies and mitogen-induced and parasite-specific proliferative T-cell responses were determined in Litomosoides sigmodontis-infected Mastomys coucha throughout an observation period of 400 days post infection (p.i.). These were compared with the respective reactions in...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s004360050018
更新日期：2000-02-01 00:00:00
abstract：:The aims of this study are to identify Leishmania species and compare and validate internal transcribed spacers (ITS) polymerase chain reaction (PCR) assay against parasitological methods for diagnosing cutaneous leishmaniasis (CL). We used the ITS-PCR, parasite culture, and microscopic evaluation of stained smears on...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-008-1111-4
更新日期：2008-10-01 00:00:00
abstract：:Hookworm infection is still a public health problem in developing countries. Aspartic protease plays an important role in parasite invasion and migration in the host. Aspartic protease gene from Ancylostoma caninum has been reported (Biochem Biophys Res Commun 227:294-302, 1996), but the activity of Acasp in eggs and ...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-009-1332-1
更新日期：2009-06-01 00:00:00
abstract：:In urban environments, catch basins serve as major developmental and resting sites for anthropophilic and zoophilic mosquitoes. However, the use of this habitat is inconsistent, with abundance of larvae varying significantly across catch basins at a fine spatial scale. During seasonal summer investigations on mosquito...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-016-4912-x
更新日期：2016-04-01 00:00:00
abstract：:Canine trypanosomosisis (CT) is a common disease caused by tsetse- and non-tsetse-transmitted trypanosomes worldwide. The severity of the disease varies from acute, sub-acute to chronic with non-specific clinical signs. Here, we attempt in a cross-sectional study to assess the current situation of CT and the role of d...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-017-5598-4
更新日期：2017-11-01 00:00:00
abstract：:A total of 116 samples (44 clinical specimens and 72 environmental samples) have been analyzed for the presence of Acanthamoeba. The environmental samples (ESs) were collected from four drinking water treatment plants (DWTP, n=32), seven wastewater treatment plants (n=28), and six locations of influence (n=12) on four...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-012-2849-2
更新日期：2012-07-01 00:00:00
abstract：:This study aimed to explore Toxoplasma gondii nucleus coding apicoplast protein acyl carrier protein (ACP) synthesis and trafficking in delayed death. The recombinant T. gondii ACP was expressed by prokaryotic expression method, and anti-ACP polyclonal antibody was obtained from rabbit immune. T. gondii "delayed death...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-014-4281-2
更新日期：2015-03-01 00:00:00
abstract：:The emergence of resistance against most of the drugs in current use against malaria has aggravated the disease burden in endemic regions. Several plants species have been used for treatment of malaria in traditional/cultural health systems. Bergenia ciliata, used traditionally for treatment of fever by local communit...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-013-3487-z
更新日期：2013-09-01 00:00:00
abstract：:In the study, we screened a total of 399 adult sheep keds (Melophagus ovinus) for the presence of RNA and DNA specific for arboviral, bacterial, and protozoan vector-borne pathogens. All investigated keds were negative for flaviviruses, phleboviruses, bunyaviruses, Borrelia burgdorferi, Rickettsia spp., Anaplasma phag...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-016-5175-2
更新日期：2016-10-01 00:00:00
abstract：:The aim of this study was to reveal genomic diversity formed during parthenogenetic reproduction of rediae of the trematode Himasthla elongata in its molluskan host Littorina littorea. We applied amplification fragment length polymorphism (AFLP) to determine the genomic diversity of individual cercariae within the clo...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-016-5249-1
更新日期：2016-12-01 00:00:00
abstract：:DNA primers were designed from the 18S rRNA sequence from the relevant digenean trematode Dicrocoelium dendriticum to evaluate a polymerase chain reaction-based diagnostic method of this parasite from its eggs in faeces of naturally and experimentally infected sheep. In order to get DNA from D. dendriticum eggs, sever...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-013-3313-7
更新日期：2013-04-01 00:00:00
abstract：:Spermiogenesis and the ultrastructure of the spermatozoon of the bothriocephalidean cestode Clestobothrium crassiceps (Rudolphi, 1819), a parasite of the teleost fish Merluccius merluccius (Linnaeus, 1758), have been studied by means of transmission electron microscopy. Spermiogenesis involves firstly the formation of...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-011-2446-9
更新日期：2012-01-01 00:00:00
abstract：:The acanthocephalan Acanthocephalus dirus is a trophically transmitted parasite that modifies both the physiology and behavior of its intermediate host (isopod) prior to transmission to its definitive host (fish). Infected isopods often contain multiple A. dirus individuals and we examined the relationships between ho...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-016-5279-8
更新日期：2017-01-01 00:00:00
abstract：:Mastomys coucha and jirds infected with Acanthocheilonema viteae, a filarial species free of endosymbiontic bacteria of the genus Wolbachia, suffer lethal side effects after effective microfilaricidal therapy with diethylcarbamazine and levamisole, whereas, M. coucha infected with the Wolbachia-infested species Brugia...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-005-1386-7
更新日期：2005-10-01 00:00:00
abstract：:Four new Ceratomyxa species were described from the gallbladder of four Red Sea fishes at Suez and Hurghada, Egypt. These species are Ceratomyxa bassoni sp. n. from Plectorhinchus gaterinus (Forsskal 1775) at Suez and Hurghada, Ceratomyxa entzerothi sp. n. from Valamugil seheli (Forsskal 1775) at Suez and Hurghada, Ce...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-008-1010-8
更新日期：2008-08-01 00:00:00
abstract：:The paucity of human cell lines expressing defined receptors for the cytoadhesion of erythrocytes infected with the human malarial parasite Plasmodium falciparumhas hampered the investigation of this important virulence property. Here, we investigate a permanent cell line derived from a human, malignant schwannoma, te...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-002-0744-y
更新日期：2003-02-01 00:00:00
abstract：:This analysis investigated the influence of breed and gender on the pharmacokinetics of monepantel, and influence of breed, age, and gender on its efficacy against gastrointestinal nematodes of sheep. In a comparison of pharmacokinetic profiles from two studies, Merino lambs had significantly greater maximum concentra...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-009-1671-y
更新日期：2010-01-01 00:00:00
