Real-time PCR for quantifying Haemonchus contortus eggs and potential limiting factors.


:The purpose of this study was to evaluate the practicality of using real-time PCR for quantifying feces-derived trichostrongyle eggs. Haemonchus contortus eggs were used to evaluate fecal contaminants, time after egg embryonation, and the presence of competing and non-competing DNAs as factors that might interfere with generating reproducible results during simplex and multiplex quantitative real-time PCR (QPCR). Real-time PCR results showed linear quantifiable amplification with DNA from five to 75 eggs. However, threshold cycle (C (T)) values obtained by amplification of DNA from egg numbers between 75 and 1,000 did not differ significantly. Inhibitors of QPCR were effectively removed during DNA extraction as exemplified by the absence of any improvement in C (T) values with bovine serum albumin or phytase treatments. Changes from egg embryonation could only be detected during the first 6 h. Noncompetitive DNA did not appear to impact amplification; however, in a multiplex reaction a competing trichostrongyle such as Cooperia oncophora can hinder amplification of H. contortus DNA, when present at tenfold greater amounts. This study demonstrates the usefulness of QPCR for amplification and quantification of trichostrongyle eggs, and identifies potential limitations, which may be addressed through multiplex assays or the addition of a standard: exogenous DNA target.


Parasitol Res


Parasitology research


Harmon AF,Williams ZB,Zarlenga DS,Hildreth MB




Has Abstract


2007-06-01 00:00:00












  • Trematode maturation patterns in a migratory snail host: What happens during upshore residency in a Mediterranean lagoon?

    abstract::Maturation of trematode larval stages is expected to be temporally and spatially adapted to maximise the encounter with the adequate downstream host, i.e. the host, which will be infected by this parasite stage. Since studies on intramolluscan parasite maturation are scarce but important in the context of parasite tra...

    journal_title:Parasitology research

    pub_type: 杂志文章


    authors: Born-Torrijos A,Raga JA,Holzer AS

    更新日期:2016-02-01 00:00:00

  • Repetitive sequences in the ITS1 region of the ribosomal DNA of Tunga penetrans and other flea species (Insecta, Siphonaptera).

    abstract::Different Tunga penetrans isolates from various hosts obtained from South America (Fortaleza. Brazil) have been studied by nucleotide sequence comparison of the first and the second internal transcribed spacer (ITS1, ITS2) of the ribosomal deoxyribonucleic acid (rDNA) and part of the mitochondrial 16S rDNA. Results sh...

    journal_title:Parasitology research

    pub_type: 杂志文章


    authors: Gamerschlag S,Mehlhorn H,Heukelbach J,Feldmeier H,D'Haese J

    更新日期:2008-01-01 00:00:00

  • Experimental Toxoplasma gondii and Eimeria tenella co-infection in chickens.

    abstract::The widespread apicomplexan parasites Toxoplasma gondii (T. gondii) and Eimeria tenella (E. tenella) are important pathogens with high prevalence in poultry. The aim of our study was the investigation of mutual influences in co-infected chickens, focusing on immune response and course of infection. Two separate trials...

    journal_title:Parasitology research

    pub_type: 杂志文章


    authors: Hiob L,Koethe M,Schares G,Goroll T,Daugschies A,Bangoura B

    更新日期:2017-11-01 00:00:00

  • The first detection of Cryptosporidium deer-like genotype in cattle in Japan.

    abstract::The general perception is that cattle are major reservoirs for Cryptosporidium parvum infections in humans and that C. parvum is a major cause of diarrhea and production loss in cattle. Adult cattle may play an important role as cryptic carrier of the infection. Cryptosporidium spp. in asymptomatic adult dairy cattle ...

    journal_title:Parasitology research

    pub_type: 杂志文章


    authors: Amer S,Honma H,Ikarashi M,Oishi R,Endo M,Otawa K,Nakai Y

    更新日期:2009-03-01 00:00:00

  • Hirudins and hirudin-like factors in Hirudinidae: implications for function and phylogenetic relationships.

    abstract::Haematophagous leeches express a broad variety of bioactive factors that are released from the salivary gland cells into the wound of a host during feeding. Among these, hirudin is probably the best studied factor and, moreover, the only one that has successfully made the transition from nature to clinical use. Many c...

    journal_title:Parasitology research

    pub_type: 杂志文章


    authors: Müller C,Haase M,Lemke S,Hildebrandt JP

    更新日期:2017-01-01 00:00:00

  • Molecular detection and identification of Ehrlichia and Anaplasma species in ixodid ticks.

    abstract::A polymerase chain reaction (PCR) assay followed by partial sequencing of the 16S ribosomal RNA gene was performed for the presence of Ehrlichia and/or Anaplasma. A total of 242 ixodid ticks were collected from domestic ruminants and their shelters, as well as humans, and their individual salivary glands were dissecte...

    journal_title:Parasitology research

    pub_type: 杂志文章


    authors: Aktas M,Altay K,Dumanli N,Kalkan A

    更新日期:2009-04-01 00:00:00

  • Inhibitory effects of novel ciprofloxacin derivatives on the growth of four Babesia species and Theileria equi.

    abstract::The problems of parasite resistance, as well as the toxic residues to most of the commercially available antipiroplasmic drugs severely weaken their effective, curative, and environmental safe employment. Therefore, it is clear that the development of treatment options for piroplasmosis is vital for improving disease ...

    journal_title:Parasitology research

    pub_type: 杂志文章


    authors: Batiha GE,Tayebwa DS,Beshbishy AM,N'Da DD,Yokoyama N,Igarashi I

    更新日期:2020-09-01 00:00:00

  • Morphological and genetic characterization of Hysterothylacium zhoushanensis sp. nov. (Ascaridida: Anisakidae) from the flatfish Pseudorhombus oligodon (Bleeker) (Pleuronectiformes: Paralichthyidae) in the East China Sea.

    abstract::Hysterothylacium zhoushanensis sp. nov. collected from the intestine of the flatfish Pseudorhombus oligodon (Bleeker) (Pleuronectiformes: Paralichthyidae) in the East China Sea is described and illustrated by light and scanning electron microscopy. The new species can be easily distinguished from its congeners by the ...

    journal_title:Parasitology research

    pub_type: 杂志文章


    authors: Li L,Liu YY,Zhang LP

    更新日期:2012-12-01 00:00:00

  • Immunoelectrophoretic analyses of antigens shared by the vesicular fluid and cyst wall of Taenia crassiceps and Taenia saginata metacestodes.

    abstract::In this study, the antigenic mosaic of the vesicular fluid (VF) and hydrosoluble cyst-wall extract (CWE) of T. crassiceps (Tc; harvested from mice) and T. saginata (Ts) metacestodes was analyzed by combined precipitation maps developed in single and bidimensional immunoelectrophoresis against the respective rabbit ant...

    journal_title:Parasitology research

    pub_type: 杂志文章


    authors: Kalinna B,Becker M,Geyer E

    更新日期:1989-01-01 00:00:00

  • Gene structure of Taenia solium paramyosin.

    abstract::Paramyosin is a muscle protein that probably plays a role in the survival of the larval stage of Taenia solium during its prolonged host-parasite relationship. Here we describe the structure of the gene coding for the paramyosin of T. solium. The characterization of two clones obtained from a genomic library showed th...

    journal_title:Parasitology research

    pub_type: 杂志文章


    authors: Vargas-Parada L,Laclette JP

    更新日期:2003-03-01 00:00:00

  • Monogenean assemblages and the apparent transmission capability of monogeneans between related fish species: an experimental study.

    abstract::Gyrodactylid monogeneans are considered to be the most invasive fish parasites. They are omnipresent on teleost fishes and host change might play a crucial role in their biology. In the present study, related fish species were kept in single- or mixed-species groups to test the ability of Gyrodactylus species to chang...

    journal_title:Parasitology research

    pub_type: 杂志文章


    authors: Blazek RD,Bagge A,Valtonen ET

    更新日期:2008-05-01 00:00:00

  • Antimalarial drug interactions of compounds isolated from Kigelia africana (Bignoniaceae) and their synergism with artemether, against the multidrug-resistant W2mef Plasmodium falciparum strain.

    abstract::For decades, drug resistance has been the major obstacle in the fight against malaria, and the search for new drugs together with the combination therapy constitutes the major approach in responding to this situation. The present study aims at assessing the in vitro antimalarial activity of four compounds isolated fro...

    journal_title:Parasitology research

    pub_type: 杂志文章


    authors: Zofou D,Tene M,Tane P,Titanji VP

    更新日期:2012-02-01 00:00:00

  • Sarcocystis neurona reacting antibodies in Missouri feral domestic cats (Felis domesticus) and their role as an intermediate host.

    abstract::Sarcocystis neurona is the parasite associated with equine protozoal myeloencephalitis (EPM). Recently, cats (Felis domesticus) have been implicated as a potential intermediate host in the life cycle of S. neurona. This study was initiated to determine whether cats have antibodies that react to S. neurona antigens sim...

    journal_title:Parasitology research

    pub_type: 杂志文章


    authors: Turay HO,Barr BC,Caldwell A,Branson KR,Cockrell MK,Marsh AE

    更新日期:2002-01-01 00:00:00

  • Purification and cellular localization of the Entamoeba histolytica transcarboxylase.

    abstract::Genome analysis of Entamoeba histolytica predicts the presence of acetyl-CoA carboxylase. Using Western blot, histochemistry, and confocal microscopy, we demonstrated the presence of a biotin-containing protein in the cytoplasm of E. histolytica, with a molecular weight of 136 kDa and biotin-carboxylase activity. This...

    journal_title:Parasitology research

    pub_type: 杂志文章


    authors: Barbosa-Cabrera E,Salas-Casas A,Rojas-Hernández S,Jarillo-Luna A,Abarca-Rojano E,Rodríguez MA,Campos-Rodríguez R

    更新日期:2012-09-01 00:00:00

  • Small ruminant resistance against gastrointestinal nematodes: a case of Haemonchus contortus.

    abstract::Gastrointestinal nematode (GIN) infections are a common constraint to small ruminant industry throughout the world, and among those, haemonchosis has its own significance. Control of GIN primarily relies on the use of anthelmintics, but this approach has become less reliable due to the development of resistance in GIN...

    journal_title:Parasitology research

    pub_type: 杂志文章,评审


    authors: Saddiqi HA,Jabbar A,Sarwar M,Iqbal Z,Muhammad G,Nisa M,Shahzad A

    更新日期:2011-12-01 00:00:00

  • Nippotaenia mogurndae Yamaguti et Myiata, 1940 (Cestoda, Nippotaeniidea): first data on spermiogenesis and sperm ultrastructure.

    abstract::Spermiogenesis and the spermatozoon ultrastructure of the cestode Nippotaenia mogurndae Yamaguti et Myiata, 1940 (Nippotaeniidea), a parasite of Perccottus glenii Dubowski, 1877 (Perciformes: Odontobutidae), have been investigated by means of transmission electron microscopy, cytochemical staining with periodic acid-t...

    journal_title:Parasitology research

    pub_type: 杂志文章


    authors: Bruňanská M,Bílý T,Nebesářová J

    更新日期:2015-04-01 00:00:00

  • Field evaluation of the efficacy of proprietary repellent formulations with IR3535 and picaridin against Aedes aegypti.

    abstract::Seven proprietary repellent formulations (3 hydro-alcoholic spray solutions and 4 skin lotions) with active ingredient IR3,535 (ethyl butylacetylaminopropionate, EBAAP) or Picaridin (hydroxyethyl isobutyl piperidine carboxylate, KBR 3,023, Bayrepel) were tested in a field study on 10 test persons over a period of 10 h...

    journal_title:Parasitology research

    pub_type: 杂志文章


    authors: Naucke TJ,Kröpke R,Benner G,Schulz J,Wittern KP,Rose A,Kröckel U,Grünewald HW

    更新日期:2007-06-01 00:00:00

  • Molecular identification of different trypanosome species in tsetse flies caught in the wildlife reserve of Santchou in the western region of Cameroon.

    abstract::Addressing the problems linked to tsetse-transmitted trypanosomiases requires considerable data on tsetse distribution and trypanosome infections. Although efforts to map tsetse and trypanosome infections have been undertaken at continental level, published data are still rare in wildlife reserves of West and Central ...

    journal_title:Parasitology research

    pub_type: 杂志文章


    authors: Kamdem CN,Tiofack AAZ,Mewamba EM,Ofon EA,Gomseu EBD,Simo G

    更新日期:2020-03-01 00:00:00

  • Hepatic Capillaria hepatica (Bancroft, 1893) infection in cat (Felis catus)-histopathological findings and first report from Iran.

    abstract::Capillaria hepatica (syn. Calodium hepaticum) is a globally distributed nematode with a high affinity to the liver of a wide range of mammalian hosts, including humans. Documented reports of the nematode in cats and associated histopathology are rare. Here, we describe a case of C. hepatica infection in a 5-year-old m...

    journal_title:Parasitology research

    pub_type: 杂志文章


    authors: Sazmand A,Khordadmehr M,Nourian A,Otranto D

    更新日期:2021-01-22 00:00:00

  • Interaction of Tetracapsuloides bryosalmonae, the causative agent of proliferative kidney disease, with host proteins in the kidney of Salmo trutta.

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    journal_title:Parasitology research

    pub_type: 杂志文章


    authors: Kumar G,Gotesman M,El-Matbouli M

    更新日期:2015-05-01 00:00:00

  • Cimex pipistrelli (Heteroptera, Cimicidae) and the dispersal propensity of bats: an experimental study.

    abstract::Previous results have shown frequent movements of crevice-dwelling bats between different shelters. Low roost fidelity of some dwelling bat species reduces the reproductive success of ectoparasites. The question of whether high densities of bat bugs (Cimex pipistrelli) represent a cost for crevice-dwelling bats (Pipis...

    journal_title:Parasitology research

    pub_type: 杂志文章


    authors: Bartonicka T

    更新日期:2008-12-01 00:00:00

  • Investigations into the production and function of leukotrienes during histotropic development of Oesophagostomum dentatum.

    abstract::The dynamics of production and excretion of leukotrienes by parasitic larvae of Oesophagostomum dentatum and their role for the development of the larvae were studied. Larvae were cultured in vitro to the fourth stage (L4). Leukotriene B4 (LTB4) was detected in homogenates and in supernatant of larvae, with the homoge...

    journal_title:Parasitology research

    pub_type: 杂志文章


    authors: Daugschies A

    更新日期:1996-01-01 00:00:00

  • Toxicity and potential utility of ivermectin and moxidectin as xenointoxicants against the common bed bug, Cimex lectularius L.

    abstract::The recent resurgence of the common bed bug Cimex lectularius L. throughout western industrialized nations has been facilitated in part by the insect becoming pesticide-resistant. Novel control strategies, including xenointoxication, should be considered to combat C. lectularius. Ivermectin, a U.S. Food and Drug Admin...

    journal_title:Parasitology research

    pub_type: 杂志文章


    authors: Sheele JM,Ridge GE

    更新日期:2016-08-01 00:00:00

  • Lyophilisation as a simple and safe method for long-term storage of free-living amoebae at ambient temperature.

    abstract::Free-living amoebae (FLA) are protozoa ubiquitously found in nature. As some species or strains of these FLA are pathogenic for humans and animals, they represent objects of medical and parasitological research worldwide. Storage of valuable FLA strains in laboratories is often time- and energy-consuming and expensive...

    journal_title:Parasitology research

    pub_type: 杂志文章


    authors: Balczun C,Scheid PL

    更新日期:2018-10-01 00:00:00

  • Trypanosoma cruzi-secreted vesicles have acid and alkaline phosphatase activities capable of increasing parasite adhesion and infection.

    abstract::Trypanosoma cruzi virulence factors include molecules expressed on the cell surface as well as those secreted or shed into the extracellular medium. Phosphatase activities modulate different aspects of T. cruzi infection, although no studies to date addressed the presence and activity of phosphatases in vesicles secre...

    journal_title:Parasitology research

    pub_type: 杂志文章


    authors: Neves RF,Fernandes AC,Meyer-Fernandes JR,Souto-Padrón T

    更新日期:2014-08-01 00:00:00

  • Oviposition deterrent and ovicidal activities of seven herbal essential oils against female adults of housefly, Musca domestica L.

    abstract::The oviposition deterrent and ovicidal of seven herbal essential oils derived from Citrus sinensis, Cymbopogon citratus, Eucalyptus glubulus, Illicium verum, Lavandula angustifolia, Mentha piperita, and Zingiber cussumunar were assessed against the gravid female of housefly, Musca domestica L., under laboratory condit...

    journal_title:Parasitology research

    pub_type: 杂志文章


    authors: Sinthusiri J,Soonwera M

    更新日期:2014-08-01 00:00:00

  • Induction of matrix metalloproteinase-9 in mice during Toxocara canis larvae migration.

    abstract::The relationships between inflammation in organs with Toxocara canis larval migration and matrix metalloproteinase-9 (MMP-9) were investigated following the infection of mice with 1,000 infective eggs. Gelatinase activity was defined by gelatin zymography, optimum pH, inhibitor specificity and Western blot analysis. M...

    journal_title:Parasitology research

    pub_type: 杂志文章


    authors: Lai SC,Chen KM,Chen HC,Lee HH

    更新日期:2005-02-01 00:00:00

  • Paramphistomum daubneyi and Fasciola hepatica: the redial burden and cercarial shedding in Lymnaea truncatula subjected to successive unimiracidial cross-exposures.

    abstract::The development of redial burden and cercarial shedding were studied in two groups of Lymnaea truncatula subjected to successive cross-exposures to one miracidium of Paramphistomum daubneyi and one of Fasciola hepatica per snail, or vice versa. The results were compared with those obtained in controls subjected to two...

    journal_title:Parasitology research

    pub_type: 杂志文章


    authors: Augot D,Abrous M,Rondelaud D,Dreyfuss G

    更新日期:1996-01-01 00:00:00

  • An in vitro model to evaluate the cytokine response in Echinococcus infections.

    abstract::With the aim of proposing an alternative model to animal experimentation, we investigated cytokine production in response to antigens in an in vitro system. This is a co-culture system of healthy human leukocytes and enterocyte-like Caco-2 cells. The antigens tested, EgA31, EgTrp, and FABP1, are candidates for vaccine...

    journal_title:Parasitology research

    pub_type: 杂志文章


    authors: Fraize M,Sarciron ME,Saboulard D,Azzouz S,Debard AL,Bosquet G,Petavy AF

    更新日期:2004-04-01 00:00:00

  • Expression of nutrient transporters in duodenum, jejunum, and ileum of Eimeria maxima-infected broiler chickens.

    abstract::The uptake of amino acids is mediated by active transporters located on the basolateral and brush border membranes of intestinal epithelial cells. The current study investigated the expression of amino acid transporters (AAT) and other genes in the intestine of chicks infected with Eimeria maxima. At 7-day postinfecti...

    journal_title:Parasitology research

    pub_type: 杂志文章


    authors: Fetterer RH,Miska KB,Jenkins MC,Wong EA

    更新日期:2014-10-01 00:00:00