Abstract:
:Anopheles campestris-like is proven to be a high-potential vector of Plasmodium vivax in Thailand. In this study, A. campestris-like salivary gland proteins were determined and analyzed by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE), two-dimensional gel electrophoresis, and nano-liquid chromatography-mass spectrometry. The total amount of salivary gland proteins in the mosquitoes aged 3-5 days was approximately 0.1 ± 0.05 μg/male and 1.38 ± 0.01 μg/female. SDS-PAGE analysis revealed at least 12 major proteins found in the female salivary glands and each morphological region of the female glands contained different major proteins. Two-dimensional gel electrophoresis showed approximately 20 major and several minor protein spots displaying relative molecular masses from 10 to 72 kDa with electric points ranging from 3.9 to 10. At least 15 glycoproteins were detected in the female glands. Similar electrophoretic protein profiles were detected comparing the male and proximal-lateral lobes of the female glands, suggesting that these lobes are responsible for sugar feeding. Blood-feeding proteins, i.e., putative 5'-nucleotidase/apyrase, anti-platelet protein, long-form D7 salivary protein, D7-related 1 protein, and gSG6, were detected in the distal-lateral lobes (DL) and/or medial lobes (ML) of the female glands. The major spots related to housekeeping proteins from other arthropod species including Culex quinquefasciatus serine/threonine-protein kinase rio3 expressed in both male and female glands, Ixodes scapularis putative sil1 expressed in DL and ML, and I. scapularis putative cyclophilin A expressed in DL. These results provide information for further study on the salivary gland proteins of A. campestris-like that are involved in hematophagy and disease transmission.
journal_name
Parasitol Resjournal_title
Parasitology researchauthors
Sor-Suwan S,Jariyapan N,Roytrakul S,Paemanee A,Saeung A,Thongsahuan S,Phattanawiboon B,Bates PA,Poovorawan Y,Choochote Wdoi
10.1007/s00436-012-3233-ysubject
Has Abstractpub_date
2013-03-01 00:00:00pages
1065-75issue
3eissn
0932-0113issn
1432-1955journal_volume
112pub_type
杂志文章abstract::Schistosomiasis affects more than 240 million people worldwide, an infection which may cause urogenital manifestations including, among others, squamous bladder cancer and prostate involvement. We describe the first case of a prostate adenocarcinoma associated with prostatic Schistosoma haematobium infection occurring...
journal_title:Parasitology research
pub_type: 杂志文章,评审
doi:10.1007/s00436-014-4250-9
更新日期:2015-02-01 00:00:00
abstract::A novel microsporidial disease was documented in two ornamental fish species, black tetra Gymnocorymbus ternetzi Boulenger 1895 and cardinal tetra Paracheirodon axelrodi Schultz 1956. The non-xenoma-forming microsporidium occurred diffusely in most internal organs and the gill, thus referring to the condition as tetra...
journal_title:Parasitology research
pub_type: 杂志文章
doi:10.1007/s00436-020-06988-7
更新日期:2021-01-08 00:00:00
abstract::This study is focused on the feasibility of two treatments of alcohol-fixed monogenean parasites which are intended to be use for the combined morphological and molecular characterizations. The monogenean parasite, Eudiplozoon nipponicum, was selected as a model parasite species; however it is expected that these tech...
journal_title:Parasitology research
pub_type: 杂志文章
doi:10.1007/s00436-010-1949-0
更新日期:2010-09-01 00:00:00
abstract::Seventy specimens of Hypsiboas albopunctatus (Anura: Hylidae) were collected and surveyed for endoparasites in Brasília, Brazil at the end of the rainy season (February and March) in 2005. Sixteen males (22.86%) were infected by Rhabdias sp., Aplectana sp., Cosmocerca sp., and Falcaustra mascula. Rhabdias sp., a lung ...
journal_title:Parasitology research
pub_type: 杂志文章
doi:10.1007/s00436-007-0797-z
更新日期:2008-03-01 00:00:00
abstract::Haplosporidian spores from Ostrea edulis, previously described as Minchinia armoricana, were reexamined by light and electron microscopy. These spores were either free among host cells or enclosed in sporocysts. They contained two long epispore cytoplasm extensions (ECE), each possessing cytoskeletal structures corres...
journal_title:Parasitology research
pub_type: 杂志文章
doi:10.1007/s004360050669
更新日期:1999-12-01 00:00:00
abstract::A molecular phylogenetic hypothesis is presented for the genus Trichuris based on sequence data from mitochondrial cytochrome c oxidase 1 (cox1) and cytochrome b (cob). The taxa consisted of nine populations of whipworm from five species of Sigmodontinae rodents from Argentina. Bayesian Inference, Maximum Parsimony, a...
journal_title:Parasitology research
pub_type: 杂志文章
doi:10.1007/s00436-016-5045-y
更新日期:2016-08-01 00:00:00
abstract::The larvicidal activities of methanolic fractions from Adhatoda vasica leaf extracts were investigated against the bancroftian filariasis vector Culex quinquefasciatus and dengue vector Aedes aegypti. The results indicated that the mortality rates was high at 100, 150, 200 and 250 ppm of methanol extract of fractions ...
journal_title:Parasitology research
pub_type: 杂志文章
doi:10.1007/s00436-011-2728-2
更新日期:2012-05-01 00:00:00
abstract::The existing armament of drugs for the treatment and prevention of malaria is inadequate due to development of resistance. In addition to this due to lack of economic enticement the rate of new drug development and new drug discovery in the segment of parasitic diseases is very low as compared to the other segments. T...
journal_title:Parasitology research
pub_type: 杂志文章
doi:10.1007/s00436-010-1789-y
更新日期:2010-05-01 00:00:00
abstract::In this study, we compared and validated a nested ABC polymerase chain reaction restriction fragment length polymorphism (PCR-RFLP) assay to amplify Cryptosporidium parvum oocyst wall protein (COWP) gene fragment with a previous nested PCR-RFLP method. When RFLP of COWP gene did not provide clear results, we further a...
journal_title:Parasitology research
pub_type: 杂志文章
doi:10.1007/s00436-008-1233-8
更新日期:2009-02-01 00:00:00
abstract::With the aim of proposing an alternative model to animal experimentation, we investigated cytokine production in response to antigens in an in vitro system. This is a co-culture system of healthy human leukocytes and enterocyte-like Caco-2 cells. The antigens tested, EgA31, EgTrp, and FABP1, are candidates for vaccine...
journal_title:Parasitology research
pub_type: 杂志文章
doi:10.1007/s00436-004-1075-y
更新日期:2004-04-01 00:00:00
abstract::The purpose of the present study was to investigate the acaricidal and larvicidal activity against the larvae of Haemaphysalis bispinosa Neumann (Acarina: Ixodidae) and larvae of hematophagous fly Hippobosca maculata Leach (Diptera: Hippoboscidae) and against the fourth-instar larvae of malaria vector, Anopheles steph...
journal_title:Parasitology research
pub_type: 杂志文章
doi:10.1007/s00436-011-2473-6
更新日期:2012-08-01 00:00:00
abstract::The distribution of actin filaments in the spermatogenic cells of Fasciola hepatica was determined using a fluorescent derivative of phalloidin. Actin was localised primarily in the region of separation of a secondary spermatogonium from a primary spermatogonium, in the inner faces at the centre of four-cell clusters ...
journal_title:Parasitology research
pub_type: 杂志文章
doi:10.1007/BF00935425
更新日期:1991-01-01 00:00:00
abstract::Metazoan parasites of guilds of benthic snails and crustaceans and of four fish families--Gobiidae, Gasterosteidae, Syngnathidae, and Zoarcidae--were investigated off the brackish Salzhaff area (Southwest Baltic) in the semienclosed Salzhaff and the near Rerik Riff in the free Baltic. Comparisons revealed greater simi...
journal_title:Parasitology research
pub_type: 杂志文章
doi:10.1007/s004360050681
更新日期:2000-05-01 00:00:00
abstract::We present a novel and simple technique for storing live Acanthamoeba for long periods of time. The amoebae are maintained at refrigerator temperatures in a peptone-yeast extract-glucose (PYG) medium normally used for cultivation. Using this method, we obtained survival rates of at least 4 years for Acanthamoeba polyp...
journal_title:Parasitology research
pub_type: 杂志文章
doi:10.1007/s00436-008-1304-x
更新日期:2009-03-01 00:00:00
abstract::The aim of this paper was to investigate the component communities of parasites in Chaetobranchopsis orbicularis from a tributary of the Amazon River system, in Northern Brazil. In 32 fish examined, 902,551 parasites were collected, including Ichthyophthirius multifiliis, Piscinoodinium pillulare, Sciadicleithrum geop...
journal_title:Parasitology research
pub_type: 杂志文章
doi:10.1007/s00436-017-5539-2
更新日期:2017-08-01 00:00:00
abstract::Two soluble Toxoplasma gondii antigen preparations were compared using the immunoblotting technique. The first preparation, which is commonly employed in ELISA tests, corresponds to a lytic extract of the parasite. The second known as exo-antigen, has yielded good results in cell immunity research and is obtained from...
journal_title:Parasitology research
pub_type: 杂志文章
doi:10.1007/BF00931730
更新日期:1992-01-01 00:00:00
abstract::The narrow Andean-Patagonian temperate rainforest strip in the west of southern South America is inhabited by two endemic species of cervids, the southern pudu (Pudu puda) and the huemul (Hippocamelus bisulcus), both cataloged as near threatened and threatened species, respectively. One of the possible causes of their...
journal_title:Parasitology research
pub_type: 杂志文章
doi:10.1007/s00436-017-5429-7
更新日期:2017-05-01 00:00:00
abstract::While Gobioidei comprises showcases of (adaptive) radiation, the scientific interest they yielded did not ensure full understanding of goby biodiversity. Even in a well-studied region like Europe, wide knowledge gaps remain. Sand gobies represent one of the few clades whose monogenean parasites have been thoroughly st...
journal_title:Parasitology research
pub_type: 杂志文章
doi:10.1007/s00436-013-3693-8
更新日期:2014-02-01 00:00:00
abstract::Aelurostrongylus abstrusus (Nematoda, Metastrongyloidea) causes verminous pneumonia in cats worldwide. This study evaluated the seroprevalence of A. abstrusus antibodies in 220 stray and free-roaming cats from insular (Mykonos, Crete, Skopelos) and continental (Thessaloniki, Attica) Greece. The results were compared w...
journal_title:Parasitology research
pub_type: 杂志文章
doi:10.1007/s00436-020-06839-5
更新日期:2020-09-01 00:00:00
abstract::Experimental infections of Galba truncatula with Fasciola hepatica were carried out under laboratory conditions to determine why the use of Tetraphyll, when provided every 3 days as food for snails, stimulated the intensity of cercarial shedding. In snails raised on cos lettuce and Tetraphyll, the number of metacercar...
journal_title:Parasitology research
pub_type: 杂志文章
doi:10.1007/s00436-004-1172-y
更新日期:2004-09-01 00:00:00
abstract::The objective of the study was to estimate the prevalence of helminths in the horse population of the state of Brandenburg, Germany. One hundred and twenty-six horse farms in the state were selected by randomised stratified sampling. In total, 1,407 horses across all farms were examined coproscopically. The experiment...
journal_title:Parasitology research
pub_type: 杂志文章
doi:10.1007/s00436-011-2362-z
更新日期:2011-05-01 00:00:00
abstract::Both the oncospheral tegument and the hook region membrane (HRM) of Echinococcus multilocularis hexacanths originate from a syncytial binucleate complex that appears in the early stage of morphogenesis and organogenesis of the hexacanth larva. The primordium of this binucleate complex forms a binucleate syncytial cap ...
journal_title:Parasitology research
pub_type: 杂志文章
doi:10.1007/s00436-018-5752-7
更新日期:2018-03-01 00:00:00
abstract::Biomphalaria glabrataembryonic (Bge) cells have been shown to provide favourable environmental conditions for the development of Schistosoma mansoni sporocysts. We investigated the effect of Bge excretory-secretory products on metabolic activity and gene transcription in S. mansoni mother sporocysts. Using the differe...
journal_title:Parasitology research
pub_type: 杂志文章
doi:10.1007/s00436-002-0643-2
更新日期:2003-01-01 00:00:00
abstract::An epidemiological and molecular survey was conducted to investigate the role of cattle in the transmission chain of cystic echinococcosis (CE) in the Campania region of southern Italy. Out of a total of 434 cattle examined for CE, 45 (10.4%) were found infected. A total of 363 cysts were collected from the infected a...
journal_title:Parasitology research
pub_type: 杂志文章
doi:10.1007/s00436-008-0948-x
更新日期:2008-06-01 00:00:00
abstract::Necrophagous Sarcophagidae are among the insects most frequently reported from human corpses. The broad forensic application of flesh flies is restricted by the lack of reliable tools for species identification of larval stages and mass breeding of collected flesh fly larvae to the adult stage, and more recently DNA-b...
journal_title:Parasitology research
pub_type: 杂志文章
doi:10.1007/s00436-015-4421-3
更新日期:2015-06-01 00:00:00
abstract::To mimic a human malaria infection in the endemic condition, two strains of mice (Balb/c and CBA) were infected and treated several times to generate so-called semi-immune status. As previously reported, neither mice (Balb/c and CBA) strain showed cerebral malaria, even in the susceptible C57BL/6 (B6). The significant...
journal_title:Parasitology research
pub_type: 杂志文章
doi:10.1007/s00436-010-2121-6
更新日期:2011-04-01 00:00:00
abstract::Macroscopically visible sarcocysts were observed in the skeletal muscles of naturally infected skinks of the genus Agama (infection rate 11.3%). Sarcocysts were described by means of transmission electron microscopy. These cysts measured 0.03-0.25 x 0.38-1.7 mm (mean 0.12 x 1.1 mm). Typical mature cysts were bordered ...
journal_title:Parasitology research
pub_type: 杂志文章
doi:10.1007/pl00008559
更新日期:2000-09-01 00:00:00
abstract::The invasive fish tapeworm, Khawia japonensis (Yamaguti, 1934) originally described in Japan, is reported for the first time in Slovakia. The tapeworm was found in farmed common carp (Cyprinus carpio L.) from East-Slovakian breeding fish pond. This finding is registered 4 years after the first announcement of this par...
journal_title:Parasitology research
pub_type: 杂志文章
doi:10.1007/s00436-015-4447-6
更新日期:2015-05-01 00:00:00
abstract::Nanotechnology is a broad interdisciplinary area of research, development, and industrial activity that has been growing rapidly worldwide for the past decade. More ambitious uses of nanoparticles are bioremediation of contaminated environments, controlled release of fragrances, biocides, and antifungals on textiles. ...
journal_title:Parasitology research
pub_type: 杂志文章,评审
doi:10.1007/s00436-008-0975-7
更新日期:2008-07-01 00:00:00
abstract::Clinostomum complanatum is an important trematode in fishes, birds of the family Ardeidae, and humans. Until now, limited knowledge is available regarding its molecular epidemiology, ecology, and phylogenetic study. Knowledge of the full mitochondrial genome of C. complanatum will provide important information for the...
journal_title:Parasitology research
pub_type: 杂志文章
doi:10.1007/s00436-016-5102-6
更新日期:2016-08-01 00:00:00