Abstract：
:With the aim of proposing an alternative model to animal experimentation, we investigated cytokine production in response to antigens in an in vitro system. This is a co-culture system of healthy human leukocytes and enterocyte-like Caco-2 cells. The antigens tested, EgA31, EgTrp, and FABP1, are candidates for vaccines in infections caused by Echinococcus spp. in the gut. All three have previously been described in the protoscolex stage and belong to protein families which confer protective immunity against several helminths. In this study, we evaluate the Th1/Th2 profile (Th1: IL-12, IFN-gamma; Th2: IL-6, IL-10) in response to protoscoleces, EgA31 and the mixture of EgA31, EgTrp and FABP1. No cytokine production was detected in response to protoscoleces. Neither IFN-gamma nor IL-6, but a significant IL-10 and IL-12 concentration was detected in response to both types of antigens. These findings suggest that EgA31 and the mixture EgA31/EgTrp/FABP1 generated an immunogenic response associated with a mixed Th1/Th2 cytokine.
journal_name
Parasitol Resjournal_title
Parasitology researchauthors
Fraize M,Sarciron ME,Saboulard D,Azzouz S,Debard AL,Bosquet G,Petavy AFdoi
10.1007/s00436-004-1075-ysubject
Has Abstractpub_date
2004-04-01 00:00:00pages
506-12issue
6eissn
0932-0113issn
1432-1955journal_volume
92pub_type
杂志文章abstract：:Gametocyte proteins are being explored as potential vaccine candidates against Eimeria sp. in chicken since they are the components of the resilient oocyst wall. The aim of this study was to investigate the immunoprophylactic efficacy of recombinant Eimeria tenella gametocyte antigen 22 (EtGam22) in chickens against h...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-018-06198-2
更新日期：2019-03-01 00:00:00
abstract：:The source of chemical anthelmintics (levamisole, flubendazole, and thiabendazole) had limited the success of gastrointestinal nematodiasis control in sheep and goats and thus awakened interest in the study of medicinal plant extracts as alternative sources of anthelmintics. The egg hatching and larvicidal effect of i...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-010-2218-y
更新日期：2011-07-01 00:00:00
abstract：:Specimens of Bertiella studeri (Cyclophyllidea, Anoplocephalidae) that have been preserved in ethanol or fixed permanently in formalin for >2 years were used for DNA extraction and molecular characterization. DNA was amplified via PCR from samples collected from different monkey and human hosts using eucestoda-specifi...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-011-2550-x
更新日期：2012-02-01 00:00:00
abstract：:In the present article, we report on the identification of Vermamoeba (Hartmannella) vermiformis as the etiological agent of a tissue infection close to the eye of a female patient. Laboratory examination revealed no involvement of any pathogenic bacteria or fungi in the tissue infection. V. vermiformis was identified...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-019-06312-y
更新日期：2019-06-01 00:00:00
abstract：:The aim of this study was to investigate the clinical efficiency of chemotherapy for the treatment of human alveolar echinococcosis (AE). Twenty-four patients who suffered from late-stage AE were enrolled in this study. The classification of the disease stages was performed according to the PNM (parasite lesion, neigh...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-013-3382-7
更新日期：2013-06-01 00:00:00
abstract：:The Asian tiger mosquito Aedes albopictus has undergone a dramatic expansion of its range in the last few decades. Since its first detection in 2007 in Germany at the motorway A5 coming from Italy via Switzerland to Germany, it has been continuously introduced by vehicles, most probably from Italy. After a hint from a...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-016-5356-z
更新日期：2017-03-01 00:00:00
abstract：:The tick Rhipicephalus microplus affects cattle health, with production loss in tropical and subtropical regions. Moreover, the use of commercial acaricides has been reduced due to the resistance of this parasite. Although alternatives such as plant bioactive molecules have been sought, essential oils present variatio...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-017-5662-0
更新日期：2018-01-01 00:00:00
abstract：:In order to assess the potential of the stem bark of Kigelia africana (Lam.) Benth as source of new anti-malarial leads, n-hexane and ethyl acetate (EtOAc) extracts and four compounds isolated from the stem bark were screened in vitro against the chloroquine-resistant W-2 and two field isolates of Plasmodium falciparu...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-011-2363-y
更新日期：2011-06-01 00:00:00
abstract：:The present study describes a transmission electron microscopical investigation of trophozoites from Giardia lamblia clone GS/M-83-H7 after exposure to monoclonal antibody (MAb) G10/4. From previous studies it is known that this antibody immunoreacts with the parasite's major surface antigen VSP (variable surface prot...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s004360050096
更新日期：1996-01-01 00:00:00
abstract：:Members of the genus Crassicauda (Nematoda: Spirurida) are parasites of the body tissues of whales and dolphins. Owing to the large size of worms and difficulties in the recovery of entire nematodes from the tissues of hosts, limited information is available on morphological descriptions of both male and female worms....
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-014-4245-6
更新日期：2015-03-01 00:00:00
abstract：:The authors previously reported that an extract from Zingiber officinale, traditionally eaten along with raw fish and used in traditional Chinese medicine, effectively destroyed Anisakis larvae in vitro. In this study, we analyzed the effective components of ginger rhizomes. Methanol extracts were fractionated after f...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/BF00931082
更新日期：1990-01-01 00:00:00
abstract：:The principal health problem in small ruminants is helminthiasis and the rapid development of nematode resistance to anthelminthics has limited the success of control in several countries, stimulating the search for alternatives. In this study, extracts of immature fruits of the mango Mangifera indica L. var Ubá were ...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-012-3017-4
更新日期：2012-12-01 00:00:00
abstract：:The present investigation was undertaken to identify and characterize trophozoite proteases of five axenic strains of Giardia duodenalis isolated in Brazil and the reference strain Portland 1 isolated in the United States. Trophozoite cell lysates of each strain were analysed for the pattern of proteins and for proteo...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-005-0124-5
更新日期：2006-07-01 00:00:00
abstract：:An in vitro system for studying the transformation of bloodstream forms of Trypanosoma brucei brucei into procylic (midgut) forms is described. In this system, transformation of the parasites was stimulated by Glossina morsitans morsitans midgut homogenates at 27 degrees C but not at 4 degrees C. The transformation-st...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/BF00936174
更新日期：1992-01-01 00:00:00
abstract：:Malarial antigens on the surface of infected erythrocytes have been described by many investigators. However, few of these antigens have been unambiguously demonstrated to be exposed on the surface of erythrocytes. This study demonstrates that mild glutaraldehyde fixation results in the cytoplasmic face of the host me...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/BF00932242
更新日期：1993-01-01 00:00:00
abstract：:With this case report, we describe Trombiculidae larvae (Neotrombicula autumnalis) infestation in a Little Bittern (Ixobrychus minutus) which was brought to our clinics by the Directorship of Environmental and Forestry authorities of Samsun, Turkey in April, 2007. The male Little Bittern (I. minutus), with a black bac...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-008-0898-3
更新日期：2008-04-01 00:00:00
abstract：:Levamisole phosphate, chosen based on its 100 % efficacy demonstrated by a previous fecal egg count reduction test (FECRT), was used as the exclusive anthelmintic treatment in the Embrapa Southeast Livestock sheep flock from 2009 to 2014 in a target selected treatment scheme. In the present study, the effectiveness of...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-015-4844-x
更新日期：2016-03-01 00:00:00
abstract：:Water buffalo is important livestock in several countries in the Latin American and Caribbean regions. This buffalo species can be infected by tick-borne hemoparasites and remains a carrier of these pathogens which represent a risk of infection for more susceptible species like cattle. Therefore, studies on the epidem...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-018-06194-6
更新日期：2019-03-01 00:00:00
abstract：:This experimental study on pigs was designed to simulate natural, long-term exposure to Ascaris suum under modern management conditions. Parasite kinetics were followed in pigs receiving A. suum eggs as repeated trickle inoculations at two dose levels beginning at a body weight of 25 kg until their slaughter at 90 kg ...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/BF00931733
更新日期：1992-01-01 00:00:00
abstract：:A comprehensive icthyoparasotological survey among estuarine and freshwater fishes of West Bengal, India revealed Trichodinella sunderbanensis sp. nov. and Trichodina acuta Lom, 1961 from an estuarine fish Mystus gulio (Hamilton, 1822) from the Vidyadhari river; Trichodina nandusi sp. nov. from a freshwater fish, Nand...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-012-3234-x
更新日期：2013-03-01 00:00:00
abstract：:Nearly 1.4 billion people in 73 countries worldwide are threatened by lymphatic filariasis, a parasitic infection that leads to a disease commonly known as elephantiasis. Filariasis is vectored by mosquitoes, with special reference to the genus Culex. The main control tool against mosquito larvae is represented by tre...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-015-4417-z
更新日期：2015-06-01 00:00:00
abstract：:Haemoproteus spp. are cosmopolitan vector-born haemosporidian parasites, some species of which cause diseases in non-adapted birds. Recent polymerase chain reaction (PCR)-based studies have detected mitochondrial cytochrome b gene lineages of these Haemoproteus parasites in blood-sucking mosquitoes and speculated abou...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-013-3375-6
更新日期：2013-06-01 00:00:00
abstract：:Experimental taeniid strobilae from Taenia solium and T. crassiceps (WFU strain) were incubated for 0-72 h in 0, 5 or 20 mM glucose solutions and further exposed for 15 min to the gap junction fluorochrome Lucifer Yellow. Frozen sections were obtained from each worm and observed under an epifluorescent microscope. Wor...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-005-1348-0
更新日期：2005-07-01 00:00:00
abstract：:The blowfly Lucilia sericata (Diptera: Calliphoridae) is a facultative ectoparasite that causes myiasis in both man and animals, leading to serious human health problems and economic losses in the livestock industry. Like other insects, olfaction of this species plays an important role in host location and is presumab...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-011-2563-5
更新日期：2012-02-01 00:00:00
abstract：:Hookworm infection is still prevalent in southern Thailand despite control measures. Hookworm eggs submerged for an extended period under water from rainfall or in latrines may not survive, but they may recover their ability to develop into infective larvae when exposed to atmospheric air. This study examined the surv...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-016-5031-4
更新日期：2016-07-01 00:00:00
abstract：:Synoptic data and an understanding of helminth parasite diversity among diverse rodent assemblages across temperate latitudes of North America remain remarkably incomplete. Renewed attention to comprehensive survey and inventory to establish the structure of biodiverse faunas is essential in providing indicators and p...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-016-5255-3
更新日期：2016-12-01 00:00:00
abstract：:Two soluble Toxoplasma gondii antigen preparations were compared using the immunoblotting technique. The first preparation, which is commonly employed in ELISA tests, corresponds to a lytic extract of the parasite. The second known as exo-antigen, has yielded good results in cell immunity research and is obtained from...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/BF00931730
更新日期：1992-01-01 00:00:00
abstract：:The inhibitory activity of mangiferin (50 mg/kg/die and 100 mg/kg/die) on Cryptosporidium parvum was evaluated in a neonatal mouse model and its activity was compared with that of paromomycin (100 mg/kg/die). At 4 days of age, neonatal Swiss conventional outbred mice were experimentally infected by oral administration...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-006-0165-4
更新日期：2006-07-01 00:00:00
abstract：:The morphology and ecology are described for four sibling species of digenean metacercariae: Meiogymnophallus minutus (Cobbold 1859), M. strigatus (Lebour 1908) from the estuarine cockle Cerastoderma edule L. and M. fossarum (Bartoli, 1965) and M. rebecqui (Bartoli, 1983) comb. nov. from the brackish water lagoon cock...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s004360050153
更新日期：1996-01-01 00:00:00
abstract：:A monoclonal antibody raised against membrane proteins of Toxoplasma gondii with molecular weights of 35 and 21 kDa also reacts strongly and "specifically" with surface antigens of Pneumocystis carinii with molecular weights of 394.2 and 69 kDa when used in a direct immunofluorescence antibody test, on the one hand, a...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/BF00930914
更新日期：1991-01-01 00:00:00
