Abstract：
:Two soluble Toxoplasma gondii antigen preparations were compared using the immunoblotting technique. The first preparation, which is commonly employed in ELISA tests, corresponds to a lytic extract of the parasite. The second known as exo-antigen, has yielded good results in cell immunity research and is obtained from Toxoplasma gondii culture supernatants. In both preparations, components exhibiting same molecular weight of 57-52 (doublet), 43, 38, 35, 30 and 20 kDa were revealed. In addition, two major 27- and 78-kDa components were detected in exo-antigens. The 30 kDa protein was intensely recognised by all sera, confirming the advantage of its use as an antigen in serological reactions. Exo-antigens could be employed as a soluble antigenic solution because they are easy to obtain, they display good antigenicity and their antigenic composition has been defined. Moreover their preparation does not require mouse inoculation. An evaluation of the enzyme-linked immunosorbent assays (ELISAs) involving these antigens has yet to be performed.
journal_name
Parasitol Resjournal_title
Parasitology researchauthors
Bessières MH,Le Breton S,Séguéla JPdoi
10.1007/BF00931730subject
Has Abstractpub_date
1992-01-01 00:00:00pages
222-8issue
3eissn
0932-0113issn
1432-1955journal_volume
78pub_type
杂志文章abstract：:High resolution two-dimensional separation of Toxoplasma gondii tachyzoite lysate revealed up to 224 distinct protein spots in Coomassie-stained gel. Computional matching of 14 digitized gels yielded a standard two-dimensional proteome map. The excretory T. gondii dense granule proteins GRA1-GRA8, S16/acid phosphatase...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s004360000367
更新日期：2001-08-01 00:00:00
abstract：:Intraspecific variation in Schistosoma mansoni infection and modulation of its expression by vertebrate host genetics was studied by evaluation of some biological parameters of the infection in BALB/c and C57BL/6 mice infected with one Brazilian (BH) and two Venezuelan (YT and SM) laboratory strains of the parasite. M...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/pl00008565
更新日期：2001-02-01 00:00:00
abstract：:Effects of gamma ray irradiation on the first-stage larvae of Angiostrongylus cantonensis were studied. Compared to the non-treated controls, infection of rats with third-stage larvae which developed from irradiated first-stage larvae resulted in reduced recovery rates of adults. There was also a change in the male-fe...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/BF00536473
更新日期：1987-01-01 00:00:00
abstract：:Entamoeba histolytica infection in humans is still one of the leading causes of parasitic diseases among the developing countries, including India. It therefore becomes extremely important to characterize this parasite with the aim of preventing and controlling the amoebiasis which it causes. The present study describ...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s004360000363
更新日期：2002-02-01 00:00:00
abstract：:The ultrastructure of alpha-bacteroids in relation to the fat-body cells of the lantern bug Pyrops candelaria was described. The fat-body-cell cytoplasm contained numerous mitochondria, rough endoplasmic reticula, vacuoles, and storage granules. Its nucleus had scattered chromatin materials. The alpha-bacteroid was en...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s004360050287
更新日期：1997-01-01 00:00:00
abstract：:Rodents are intermediate hosts for many species of Sarcocystis. Little is known of Sarcocystis cymruensis that uses the Brown rat (Rattus norvegicus) as intermediate hosts and the domestic cat (Felis catus) as experimental definitive host. Here, we identified and described Sarcocystis cymruensis in naturally infected ...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-018-5799-5
更新日期：2018-04-01 00:00:00
abstract：:An easy and rapid tool for the individual identification of Haemonchus species in domestic ruminants might be very useful in the study of natural populations, especially when two (or three) species are sympatric. Techniques based on cytology, cuticular-ridge patterns, sublateral hypodermic chords, or molecular biology...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s004360050213
更新日期：1997-01-01 00:00:00
abstract：:The var-gene encoding Plasmodium falciparum erythrocyte membrane protein 1 (PfEMP1) is known to play a major role in the pathogenicity of the P. falciparum parasite. The protein enables the parasite to adhere to the endothelial linings of small blood vessels (cytoadherence) as well as to non-infected erythrocytes (ros...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-009-1583-x
更新日期：2009-11-01 00:00:00
abstract：:The C57BL/6J strain of Mus musculus is susceptible to the terrestrial trematode Brachylaima cribbi. The duration of infection in these mice is generally 9-12 weeks with a peak excretion of eggs at 4 weeks post-infection (wpi). The effects of age and sex on the course of infection were investigated by comparing infecti...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-002-0642-3
更新日期：2002-07-01 00:00:00
abstract：:The efficacy of emodepside plus toltrazuril (Procox® oral suspension for dogs) against different species of gastrointestinal nematodes (Toxocara canis, Ancylostoma caninum, Uncinaria stenocephala) was evaluated in nine randomised,blinded and placebo-controlled laboratory studies in naturally or experimentally infected...
journal_title：Parasitology research
pub_type： 杂志文章,随机对照试验
doi：10.1007/s00436-011-2397-1
更新日期：2011-08-01 00:00:00
abstract：:Spirocerca vulpis is a parasitic nematode of red foxes associated with gastric nodule formation. Since its description in 2018, this species has been reported in red foxes from Spain, Bosnia and Herzegovina, Italy, and Portugal. We present here the analysis of uncharacterized nematodes obtained from gastric nodules of...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-020-06791-4
更新日期：2020-09-01 00:00:00
abstract：:Cryptosporidiosis, caused by Cryptosporidium, causes self-limited diarrhea in normal hosts but may cause life-threatening diarrhea in immunocompromised persons. Cryptosporidium-induced manifestations, including weight-loss and intestinal physiological alterations are not noted in adult immunocompetent mice. So far, st...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-007-0785-3
更新日期：2008-02-01 00:00:00
abstract：:Passive transfer of immunity using a mouse monoclonal IgE antibody against Schistosoma japonicum was found to be enhanced by heterologous helminth infections. BALB/c mice were infected with Toxocara canis or Nippostrongylus brasiliensis so as to induce eosinophilia prior to a challenge infection with S. japonicum. Rec...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/BF00928681
更新日期：1991-01-01 00:00:00
abstract：:Analyses were made on the adenosine transporter-1 gene in Trypanosoma brucei (TbAT1), encoding a P2-like nucleoside transporter, from T. brucei brucei field stocks to investigate a possible link between the presence of mutations in this gene and isometamidium resistance. We have analysed the gene from 11 isometamidium...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-006-0141-z
更新日期：2006-08-01 00:00:00
abstract：:Feasibility of implementing a DEC-fortified (DEC at 0.2% w/w and iodine) salt strategy to hasten elimination of diurnally sub-periodic Wuchereria bancrofti (DspWB) from the lone foci in Nancowry islands, Nicobar district, India, was assessed. This is a two-arm community-based study: one arm (12 villages, population 29...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-020-06659-7
更新日期：2020-05-01 00:00:00
abstract：:Knowledge of species and genotypes on Cryptosporidium involved in human transmission is fundamental in studying the epidemiology of cryptosporidiosis. This is the first study in molecular epidemiology of cryptosporidiosis that has been made in Chile. Cryptosporidium parvum species and genotypes involved in human and b...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-005-1391-x
更新日期：2005-08-01 00:00:00
abstract：:To determine the prevalence of endoparasites and their association with diarrhea, a survey was conducted in the Southern Province of Zambia that used conventional and molecular techniques applied to stool and urine samples from school-age children (n = 93). Almost half of the stools (49.5%) were diarrhetic. The overal...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-005-0003-0
更新日期：2005-12-01 00:00:00
abstract：:The existing armament of drugs for the treatment and prevention of malaria is inadequate due to development of resistance. In addition to this due to lack of economic enticement the rate of new drug development and new drug discovery in the segment of parasitic diseases is very low as compared to the other segments. T...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-010-1789-y
更新日期：2010-05-01 00:00:00
abstract：:The epidemiological importance of increasing reports worldwide on Cryptosporidium contamination of oysters remains unknown in relation to foodborne cryptosporidiosis. Thirty market-size oysters (Crassostrea virginica), collected from each of 53 commercial harvesting sites in Chesapeake Bay, MD, were quantitatively tes...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-006-0261-5
更新日期：2007-01-01 00:00:00
abstract：:During our investigations on helminthofauna in roe deer (Capreolus capreolus) and red deer (Cervus elaphus) in France (1985-1998) we isolated nematodes not only of the genus Haemonchus Cobb, 1898 but also of the genus Ashworthius Le Roux 1930, both of which belong to the same subfamily of Haemonchinae. The prevalence ...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/pl00008534
更新日期：2000-07-01 00:00:00
abstract：:In July 2005, 107 rainbow trout in age 1+ from a salmonid farm in Southern Germany situated in the southern tributary area of the Danube river were examined. The aim of this study was to determine the gyrodactylid species found on rainbow trout and to identify their location on the host's body. In total, 291 specimens...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-008-1244-5
更新日期：2009-02-01 00:00:00
abstract：:This study investigates the occurrence of anisakids and raphidascarids in commercial fish from Balearic Sea (Western Mediterranean). A total of 335 fish including 19 black anglerfish (Lophius budegassa), 33 white anglerfish (L. piscatorius), 129 European hake (Merluccius merluccius), 30 red mullet (Mullus barbatus), a...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-018-6110-5
更新日期：2018-12-01 00:00:00
abstract：:Three in vitro studies were designed to develop an assay for anticoccidial efficacy by use of laboratory (Houghton) and field (T-376) Eimeria tenella strains. In study (1), minimum inhibitory concentrations (MICs) of monensin (Mon), maduramicin (Mad), salinomycin (Sal), and lasalocid (Las) were determined that are abl...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-015-4404-4
更新日期：2015-06-01 00:00:00
abstract：:Antroponotic cutaneous leishmaniasis (ACL) is an endemic disease and one of the major health problems in Sanliurfa province located in the southeastern region of Turkey. Leishmania tropica is confirmed as the causative agent of ACL in this region. In Sanliurfa city alone, the recorded total cases of ACL were 6,817 bet...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-008-1013-5
更新日期：2008-08-01 00:00:00
abstract：:Cyclospora cayetanensis was identified as the cause of large outbreaks of diarrhea in many parts of the world, but its host range and reservoirs remains poorly defined. Recently, oocysts resembling the C. cayetanensis were detected in dairy cattle fecal specimens from China. The 18S rDNA from two of these Cyclospora-l...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-006-0380-z
更新日期：2007-04-01 00:00:00
abstract：:The apparent scarcity or absence of blood parasites in some avian groups, such as seabirds, has been related to intrinsic and extrinsic factors including host immunological capacity, host-parasite assemblage, and ecological parameters, but also to reduced sensitivity of some methods to detect low parasite prevalence/i...
journal_title：Parasitology research
pub_type： 杂志文章,评审
doi：10.1007/s00436-017-5676-7
更新日期：2018-01-01 00:00:00
abstract：:Three actinosporean types--raabeia, aurantiactinomyxon, and guyenotia--from the oligochaete Branchiura sowerbyi Beddard collected from a crucian carp (Carassius auratus gibelio) pond are described in this report. Compared with the actinospores described previously, the raabeia type presents similar spore shape with th...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-013-3306-6
更新日期：2013-04-01 00:00:00
abstract：:The aim of this paper was to investigate the component communities of parasites in Chaetobranchopsis orbicularis from a tributary of the Amazon River system, in Northern Brazil. In 32 fish examined, 902,551 parasites were collected, including Ichthyophthirius multifiliis, Piscinoodinium pillulare, Sciadicleithrum geop...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-017-5539-2
更新日期：2017-08-01 00:00:00
abstract：:The sequence, genomic organization, and transcription of the gene encoding the H2A histone protein of the protozoan parasite Trypanosoma rangeli is described in this paper. The locus encoding the T. rangeli H2A protein is formed by at least 11 gene units measuring 790 nucleotides in length, organized in tandem, and lo...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s004360000270
更新日期：2000-11-01 00:00:00
abstract：:In three different areas in northern Germany, hippoboscids were collected from red deer (Cervus elaphus). Typanosomatids were demonstrated in the midgut and hindgut of 9/37 Lipoptena cervi. The trypanosomatids were morphologically similar to vector forms of the subgenus Megatrypanum that had been found in tabanids at ...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/BF00928691
更新日期：1991-01-01 00:00:00
