Abstract：
:Non-invasive diagnoses for most of the extraintestinal helminth infections among vertebrates are rare. In the present study, we developed and tested a non-invasive visual diagnosis method to detect the infection of the nematode Philometra ovata, which is parasitising in the body cavity of the common minnow Phoxinus phoxinus. By observing the surface of minnow's abdomen, we diagnosed infected minnows with irregular or vermiform-shaped uplifts as a consequence of the presence of P. ovata in the body cavity. We conducted the diagnosis in minnows with or without anaesthetisation, and our results showed the non-invasive method is highly reliable (correct rate of diagnoses > 95 %) in both anaesthetised and non-anaesthetised groups. The correct rate of diagnoses in truly uninfected fish (i.e. specificity) was nearly 100 % in both groups, while the correct rate of diagnoses in truly infected fish (i.e. sensitivity) was 71 and 65 % in anaesthetised and non-anaesthetised fish, respectively. The correct rate in fish diagnosed as infected or uninfected (i.e. positive or negative predictive power) of non-invasive diagnosis was nearly 100 % among the anaesthetised fish, and over 95 % in non-anaesthetised group. The diagnosis also yielded prevalence of P. ovata infection similar to the real prevalence in anaesthetised fish. Diagnoses conducted by an inexperienced observer indicated that the method is repeatable. Taken together, the present non-invasive method seems to be a promising new tool for non-invasive detection of P. ovata infections in minnows and probably can be applied for the detection of other body cavity dwelling metazoan parasites in various host taxa.
journal_name
Parasitol Resjournal_title
Parasitology researchauthors
Lai YT,Taskinen J,Kekäläinen J,Kortet Rdoi
10.1007/s00436-012-3099-zsubject
Has Abstractpub_date
2012-12-01 00:00:00pages
2411-8issue
6eissn
0932-0113issn
1432-1955journal_volume
111pub_type
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