Non-invasive diagnosis for Philometra ovata (Nematoda) infection in the common minnow Phoxinus phoxinus.


:Non-invasive diagnoses for most of the extraintestinal helminth infections among vertebrates are rare. In the present study, we developed and tested a non-invasive visual diagnosis method to detect the infection of the nematode Philometra ovata, which is parasitising in the body cavity of the common minnow Phoxinus phoxinus. By observing the surface of minnow's abdomen, we diagnosed infected minnows with irregular or vermiform-shaped uplifts as a consequence of the presence of P. ovata in the body cavity. We conducted the diagnosis in minnows with or without anaesthetisation, and our results showed the non-invasive method is highly reliable (correct rate of diagnoses > 95 %) in both anaesthetised and non-anaesthetised groups. The correct rate of diagnoses in truly uninfected fish (i.e. specificity) was nearly 100 % in both groups, while the correct rate of diagnoses in truly infected fish (i.e. sensitivity) was 71 and 65 % in anaesthetised and non-anaesthetised fish, respectively. The correct rate in fish diagnosed as infected or uninfected (i.e. positive or negative predictive power) of non-invasive diagnosis was nearly 100 % among the anaesthetised fish, and over 95 % in non-anaesthetised group. The diagnosis also yielded prevalence of P. ovata infection similar to the real prevalence in anaesthetised fish. Diagnoses conducted by an inexperienced observer indicated that the method is repeatable. Taken together, the present non-invasive method seems to be a promising new tool for non-invasive detection of P. ovata infections in minnows and probably can be applied for the detection of other body cavity dwelling metazoan parasites in various host taxa.


Parasitol Res


Parasitology research


Lai YT,Taskinen J,Kekäläinen J,Kortet R




Has Abstract


2012-12-01 00:00:00












  • Identification of hard ticks (Acari: Ixodidae) and seroprevalence to Theileria parva in cattle raised in North Kivu Province, Democratic Republic of Congo.

    abstract::This study aimed to identify tick species and to determine their relationship with the Theileria parva seroprevalence in cattle raised under an extensive farming system in North Kivu Province, Democratic Republic of Congo in two agro-ecological zones namely medium (1,000-1,850 m) and high (>1,850 m) altitude. Among th...

    journal_title:Parasitology research

    pub_type: 杂志文章


    authors: Kalume MK,Saegerman C,Mbahikyavolo DK,Makumyaviri AM,Marcotty T,Madder M,Caron Y,Lempereur L,Losson B

    更新日期:2013-02-01 00:00:00

  • Changes of the abundance of Culicoides obsoletus s.s. and Culicoides scoticus in Southwest Germany identified by a PCR-based differentiation.

    abstract::The outbreak of bluetongue disease in Central Europe necessitates new approaches in the identification of vectors to follow-up changes of populations of species and not of complexes. Since females of species of the complex of Culicoides obsoletus are difficult to be identified according to morphological criteria, we a...

    journal_title:Parasitology research

    pub_type: 杂志文章


    authors: Balczun C,Vorsprach B,Meiser CK,Schaub GA

    更新日期:2009-08-01 00:00:00

  • Freeze-fracture studies on Pneumocystis carinii. II. Fine structure of the trophozoite.

    abstract::Ultrastructure of the trophozoite of Pneumocystis carinii was studied by the freeze-fracture technique. Nuclei and cytoplasmic organelles such as the endoplasmic reticulum, mitochondria, cytoplasmic vacuoles and small round bodies were observed. The mean number of nuclear pores was 8 per micron 2, which is small compa...

    journal_title:Parasitology research

    pub_type: 杂志文章


    authors: Yoshikawa H,Morioka H,Yoshida Y

    更新日期:1987-01-01 00:00:00

  • Avian and simian malaria: do they have a cancer connection?

    abstract::It has been claimed that infectious agents transmitted by mosquitoes (Diptera: Culicidae) may have a greater connection to cancer then hitherto supposed and that the immune system struggles to recognize and fight some of these infectious agents. One of the claims made is that there is a connection between human malari...

    journal_title:Parasitology research

    pub_type: 杂志文章,评审


    authors: Ward M,Benelli G

    更新日期:2017-03-01 00:00:00

  • Biological activity of Paecilomyces genus against Toxocara canis eggs.

    abstract::Saprophytic soil fungi can exert ovicidal and ovistatic effects on helminths with differing degrees of efficiency. The representatives of such fungi from temperate regions, Paecilomyces lilacinus (Thom) Samson and P. marquandii (Masse) Hughes, exhibit recognized ovicidal activity on some nematodes. We evaluated the ac...

    journal_title:Parasitology research

    pub_type: 杂志文章


    authors: Basualdo JA,Ciarmela ML,Sarmiento PL,Minvielle MC

    更新日期:2000-10-01 00:00:00

  • Phylogenetic characterization of Setaria equina and its association with other filarids.

    abstract::Molecular characterization studies on Setaria equina are limited. The present study aimed to characterize S. equina at the cytochrome c oxidase gene and to examine its phylogenetic relationships with other filarid species. Sequence analysis showed 100% nucleotide homology with an S. equina sequence from Italy (AJ54487...

    journal_title:Parasitology research

    pub_type: 杂志文章


    authors: Devi A,Sudan V,Shanker D

    更新日期:2020-12-01 00:00:00

  • Molecular characterization of the parasitic tapeworm Bertiella studeri from the island of Mauritius.

    abstract::Specimens of Bertiella studeri (Cyclophyllidea, Anoplocephalidae) that have been preserved in ethanol or fixed permanently in formalin for >2 years were used for DNA extraction and molecular characterization. DNA was amplified via PCR from samples collected from different monkey and human hosts using eucestoda-specifi...

    journal_title:Parasitology research

    pub_type: 杂志文章


    authors: Taleb-Hossenkhan N,Bhagwant S

    更新日期:2012-02-01 00:00:00

  • Mapping the genome of Eimeria tenella: use of a yeast artificial chromosome library as the basis for a physical map.

    abstract::Efforts to derive a physical map of the genome of Eimeria tenella are being made to complement genetic mapping and to assist in the production of an integrated map. A large insert library of DNA from the Houghton (H) reference strain of E. tenella has been constructed in yeast artificial chromosomes (YACs). The librar...

    journal_title:Parasitology research

    pub_type: 杂志文章


    authors: Hoogendoorn B,Toye AA,Bumstead N,Shirley MW

    更新日期:1998-01-01 00:00:00

  • The first genotype determination of Acanthamoeba potential threat to human health, isolated from natural water reservoirs in Poland.

    abstract::Different species of amoebae belonging to the genus Acanthamoeba are widely distributed in many parts of the world and known as free-living organisms. Some strains of the protozoans may exist as parasites and cause risk to human health as causative agents of serious human diseases. Currently, in Poland, there is no su...

    journal_title:Parasitology research

    pub_type: 杂志文章


    authors: Lass A,Szostakowska B,Idzińska A,Chomicz L

    更新日期:2014-07-01 00:00:00

  • Real-time PCR for quantifying Haemonchus contortus eggs and potential limiting factors.

    abstract::The purpose of this study was to evaluate the practicality of using real-time PCR for quantifying feces-derived trichostrongyle eggs. Haemonchus contortus eggs were used to evaluate fecal contaminants, time after egg embryonation, and the presence of competing and non-competing DNAs as factors that might interfere wit...

    journal_title:Parasitology research

    pub_type: 杂志文章


    authors: Harmon AF,Williams ZB,Zarlenga DS,Hildreth MB

    更新日期:2007-06-01 00:00:00

  • Ultrastructure of the body wall of Cystidicoloides ephemeridarum (Nematoda, Cystidicolidae) in relation to the histopathology of this nematode in salmonids.

    abstract::Light, scanning and transmission electron microscope examinations of adult Cystidicoloides ephemeridarum nematodes, parasitic in the stomach of brown trout (Salmo trutta fario L.), revealed that the body wall is composed of a cuticle, a hypodermis and longitudinally oriented somatic musculature. The body cuticle is co...

    journal_title:Parasitology research

    pub_type: 杂志文章


    authors: Frantová D,Moravec F

    更新日期:2003-09-01 00:00:00

  • Do parasites influence behavioural traits of wild and hatchery-reared Murray cod, Maccullochella peelii?

    abstract::This study aimed to investigate the links between parasites and behavioural traits of juvenile Murray cod (Maccullochella peelii). The Murray cod is an endangered Australian freshwater fish for which restocking programs are in place and there is a growing human consumption market. However, little is known about the pa...

    journal_title:Parasitology research

    pub_type: 杂志文章


    authors: Shamsi S,Rogers L,Sales E,Kopf RK,Freire R

    更新日期:2021-01-06 00:00:00

  • Coccidia of Columbiformes: a taxonomic review of its Eimeriidae species and Eimeria columbinae n. sp. from Columbina talpacoti (Temminck, 1809) from Brazil.

    abstract::Coccidia (Chromista: Miozoa: Eimeriidae) of columbiform birds (Aves: Columbiformes) have been described since the end of the nineteenth century; however, some of these descriptions were poorly detailed or inconclusive. In this sense, the current work makes a detailed taxonomic revision reconsidering and organizing 18 ...

    journal_title:Parasitology research

    pub_type: 杂志文章


    authors: Ortúzar-Ferreira CN,Oliveira MS,Genovez-Oliveira JL,Franco HA,Thode-Filho S,Cardozo SV,Oliveira ÁA,Lima VM,Ferreira I,Berto BP

    更新日期:2020-01-01 00:00:00

  • Molecular characterization of Leishamania isolates from China by inter-simple sequence repeat polymerase chain reaction.

    abstract::Leishmania has distinct epidemiological and biological characteristics and causes a variety of clinical symptoms. To understand the genetic diversity and the phylogenetic relationships among Leishmania isolates from China, 29 Leishmania isolates from different geographic origins, vectors, and hosts were analyzed using...

    journal_title:Parasitology research

    pub_type: 杂志文章


    authors: Wang Y,Yang Y,Wang J,Bao Y,Guan L,Gao C,Shi F

    更新日期:2010-05-01 00:00:00

  • Molecular identification of different trypanosome species in tsetse flies caught in the wildlife reserve of Santchou in the western region of Cameroon.

    abstract::Addressing the problems linked to tsetse-transmitted trypanosomiases requires considerable data on tsetse distribution and trypanosome infections. Although efforts to map tsetse and trypanosome infections have been undertaken at continental level, published data are still rare in wildlife reserves of West and Central ...

    journal_title:Parasitology research

    pub_type: 杂志文章


    authors: Kamdem CN,Tiofack AAZ,Mewamba EM,Ofon EA,Gomseu EBD,Simo G

    更新日期:2020-03-01 00:00:00

  • Predominance of Blastocystis sp. subtype 4 in rural communities, Nepal.

    abstract::Blastocystis sp. is a common intestinal parasite. To date, there have been sporadic and scanty studies on Blastocystis sp. carried out in rural communities in Nepal. We surveyed the prevalence of Blastocystis sp. and its possible associated risk factors, and reported the predominant Blastocystis sp. subtype in two rur...

    journal_title:Parasitology research

    pub_type: 杂志文章


    authors: Lee IL,Tan TC,Tan PC,Nanthiney DR,Biraj MK,Surendra KM,Suresh KG

    更新日期:2012-04-01 00:00:00

  • Multicomponent DNA vaccine-encoding Toxoplasma gondii GRA1 and SAG1 primes: anti-Toxoplasma immune response in mice.

    abstract::A multicomponent DNA vaccine, encoding Toxoplasma gondii GRA1 and SAG1, was constructed and tested for its ability to confer protection. BALB/c mice were challenged with tachyzoites of the virulent T. gondii RH strain at 4 weeks following the last immunization, and immune responses and survival times were observed. Th...

    journal_title:Parasitology research

    pub_type: 杂志文章


    authors: Wu XN,Lin J,Lin X,Chen J,Chen ZL,Lin JY

    更新日期:2012-11-01 00:00:00

  • Clinical implications of a gradual dormancy concept in malaria.

    abstract::Malaria recurrences after an initially successful therapy and malarial fever occurring a long time after infection are well-known problems in malariology. Currently, two distinct types of malaria recurrences are defined: recrudescence and relapse. A recrudescence is thought to originate from circulating Plasmodium blo...

    journal_title:Parasitology research

    pub_type: 杂志文章,评审


    authors: Richter J,Franken G,Holtfreter MC,Walter S,Labisch A,Mehlhorn H

    更新日期:2016-06-01 00:00:00

  • Prevalence and distribution of Cryptosporidium spp. in dairy cattle in Heilongjiang Province, China.

    abstract::Few data are available on the molecular characterization of Cryptosporidium spp. in cattle in China. In the present study, a total of 507 fecal specimens from six dairy farms in Heilongjiang Province were examined for Cryptosporidium spp. by light microscopy of concentrates from the formalin-ethyl acetate sedimentatio...

    journal_title:Parasitology research

    pub_type: 杂志文章


    authors: Liu A,Wang R,Li Y,Zhang L,Shu J,Zhang W,Feng Y,Xiao L,Ling H

    更新日期:2009-09-01 00:00:00

  • Purification and identification of a neutral endopeptidase in Plasmodium falciparum schizonts and merozoites.

    abstract::An endopeptidase specific to the Plasmodium falciparum erythrocytic schizont stage and to free merozoites was detected using the fluorogenic GlcA-Val-Leu-Gly-Lys(or Arg)-AEC substrate. The enzyme was purified by high performance liquid chromatography (HPLC); its optimal activity was around pH 7.5 and its isoelectric p...

    journal_title:Parasitology research

    pub_type: 杂志文章


    authors: Grellier P,Picard I,Bernard F,Mayer R,Heidrich HG,Monsigny M,Schrével J

    更新日期:1989-01-01 00:00:00

  • Structure of parasites community in Chaetobranchopsis orbicularis (Cichlidae), a host from the Amazon River system in northern Brazil.

    abstract::The aim of this paper was to investigate the component communities of parasites in Chaetobranchopsis orbicularis from a tributary of the Amazon River system, in Northern Brazil. In 32 fish examined, 902,551 parasites were collected, including Ichthyophthirius multifiliis, Piscinoodinium pillulare, Sciadicleithrum geop...

    journal_title:Parasitology research

    pub_type: 杂志文章


    authors: Tavares-Dias M,Oliveira MSB

    更新日期:2017-08-01 00:00:00

  • The effect of a taste-enhancement process for cold-stored raw shell-stock oysters (Crassostrea virginica) on the spillage of human enteropathogens.

    abstract::A taste-enhancement process for cold-stored, raw shell-stock Crassostrea virginica oysters (i.e., application of table salt to shells) when externally contaminated with human enteropathogens intensified spillage of these enteropathogens to oyster' storage containers (77% compared to 27% for controls) but did not, howe...

    journal_title:Parasitology research

    pub_type: 杂志文章


    authors: Graczyk TK,Tamang L,Pelz R

    更新日期:2007-11-01 00:00:00

  • Morphology of the alimentary tract of Trichinella spiralis muscle larvae with emphasis on the esophagus.

    abstract::This study was designed to provide a comprehensive description of the ultrastructure of the esophagus of Trichinella spiralis muscle larvae. Although the esophagus exhibited basically the same structure throughout its entire length, being composed of a single cell-layered epithelium, the basal lamina, and the cuticle,...

    journal_title:Parasitology research

    pub_type: 杂志文章


    authors: Takahashi Y,Uno T,Yagi J,Yamada S,Araki T

    更新日期:1988-01-01 00:00:00

  • Trombiculidae larvae (Neotrombicula autumnalis) infestation in a Little Bittern (Ixobrychus minutus) in Turkey.

    abstract::With this case report, we describe Trombiculidae larvae (Neotrombicula autumnalis) infestation in a Little Bittern (Ixobrychus minutus) which was brought to our clinics by the Directorship of Environmental and Forestry authorities of Samsun, Turkey in April, 2007. The male Little Bittern (I. minutus), with a black bac...

    journal_title:Parasitology research

    pub_type: 杂志文章


    authors: Cakiroglu D,Pekmezci D,Meral Y,Gokalp G,Acici M

    更新日期:2008-04-01 00:00:00

  • Removal of hydrogen peroxide by a 1-cysteine peroxiredoxin enzyme of the filarial parasite Dirofilaria immitis.

    abstract::Prior studies have shown that filarial nematodes can effectively metabolize hydrogen peroxide in excess of that generated by activated host cells. However, the mechanisms of H2O2 removal by the filarial parasites are unclear. Herein we report the results of studies carried out on the biochemical activity and on immuno...

    journal_title:Parasitology research

    pub_type: 杂志文章


    authors: Chandrashekar R,Tsuji N,Morales TH,Carmody AB,Ozols VO,Welton J,Tang L

    更新日期:2000-03-01 00:00:00

  • Climate, niche, ticks, and models: what they are and how we should interpret them.

    abstract::Ticks spend most of their life cycle in the environment, and all tick life cycle stages are dependent on a complex combination of climate variables. Furthermore, host availability and vegetation significantly modulate the dynamics of tick populations. Tick recruitment is dependent on successful reproduction, which in ...

    journal_title:Parasitology research

    pub_type: 杂志文章,评审


    authors: Estrada-Peña A

    更新日期:2008-12-01 00:00:00

  • Ultrastructural alterations induced by nifurtimox and another nitro derivative on epimastigotes of Trypanosoma cruzi.

    abstract::We report the ultrastructural alterations induced on epimastigotes by nifurtimox and 5-nitro-2-thienyl-malononitrile (5NO2TM), a novel compound with anti-Trypanosoma cruzi activity. Parasites treated with concentrations of nifurtimox lower than usually employed for this kind of study showed vacuolisation, alterations ...

    journal_title:Parasitology research

    pub_type: 杂志文章


    authors: Muelas-Serrano S,Pérez-Serrano J,Gómez-Barrio A,Arán VJ,Rodríguez-Caabeiro F

    更新日期:2002-02-01 00:00:00

  • Design of protease-resistant pexiganan enhances antileishmanial activity.

    abstract::Antimicrobial peptides are increasingly being explored as alternative agents for therapy against the parasitic protozoan Leishmania. Previously, we reported that the synthetic magainin analog, pexiganan, induced apoptosis of surface protease-deficient Leishmania. Here, we report the development of an arginine-rich var...

    journal_title:Parasitology research

    pub_type: 杂志文章


    authors: Kulkarni MM,Karafova A,Kamysz W,McGwire BS

    更新日期:2014-05-01 00:00:00

  • Toxoplasma gondii: redistribution of tachyzoite surface protein during host cell invasion and intracellular development.

    abstract::Immunoperoxidase localisation of antigen at the electron microscope level confirms that parasite surface proteins, in association with membrane, are shed from the surface of the zoite on invasion, while varying amounts are also internalised. SAG 1 is stable on intracellular zoites for up to 48 h, although new protein ...

    journal_title:Parasitology research

    pub_type: 杂志文章


    authors: Grimwood J,Smith JE

    更新日期:1995-01-01 00:00:00

  • Formation by the uterus of a peripheral layer of the sheath in microfilariae of Litomosoides carinii and Brugia malayi.

    abstract::The eggshells of young developmental stages in the uterus are rather thin and homogenous. In the brezel stage of Brugia malayi they are 35 nm thick and 20 nm in Litomosoides carinii. In young developmental stages up to brezel stages the eggshells bind the lectins WGA, DBA and PNA labelled with colloidal gold. This sho...

    journal_title:Parasitology research

    pub_type: 杂志文章


    authors: Schraermeyer U,Peters W,Zahner H

    更新日期:1987-01-01 00:00:00