Molecular survey of arthropod-borne pathogens in sheep keds (Melophagus ovinus), Central Europe.

Abstract:

:In the study, we screened a total of 399 adult sheep keds (Melophagus ovinus) for the presence of RNA and DNA specific for arboviral, bacterial, and protozoan vector-borne pathogens. All investigated keds were negative for flaviviruses, phleboviruses, bunyaviruses, Borrelia burgdorferi, Rickettsia spp., Anaplasma phagocytophilum, "Candidatus Neoehrlichia mikurensis," and Babesia spp. All ked pools were positive for Bartonella DNA. The sequencing of the amplified fragments of the gltA and 16S-23S rRNA demonstrated a 100 % homology with Bartonella melophagi previously isolated from a sheep ked and from human blood in the USA. The identification of B. melophagi in sheep keds in Central Europe highlights needs extending a list of hematophagous arthropods beyond ticks and mosquitoes for a search of emerging arthropod-borne pathogens.

journal_name

Parasitol Res

journal_title

Parasitology research

authors

Rudolf I,Betášová L,Bischof V,Venclíková K,Blažejová H,Mendel J,Hubálek Z,Kosoy M

doi

10.1007/s00436-016-5175-2

subject

Has Abstract

pub_date

2016-10-01 00:00:00

pages

3679-82

issue

10

eissn

0932-0113

issn

1432-1955

pii

10.1007/s00436-016-5175-2

journal_volume

115

pub_type

杂志文章