Abstract：
:Antigens derived from Echinococcus granulosus, Taenia hydatigena and T. pisiformis cyst fluids, T. solium cysticerci, E. multilocularis protoscoleces and E. vogeli cyst membranes were examined by enzyme-linked immunosorbent assay (ELISA) and immunoelectrophoresis (IEP) using four monoclonal antibodies (mAb) to E. granulosus antigen 5 (Ag5) and antigen B (AgB). Anti-Ag5 mAbs 24.14 and 61A12 reacted strongly with T. hydatigena and T. pisiformis cyst fluids and, to a lesser degree, anti-AgB MAbs 31.15 and 39B3 also displayed some reaction with these antigens in ELISA. The formation of a modified arc 5 band between Anti-Ag5 mAbs and T. hydatigena cyst fluid (THCF) in IEP further confirms the existence of Ag5 in T. hydatigena cyst fluid. However, the inability of THCF and T. pisiformis cyst fluid (TPCF) to form an AgB band as well as that of TPCF to form an arc 5 band with mAbs in IEP does not exclusively prove the lack of AgB in THCF and TPCF or the lack of Ag5 in TPCF. The absence of a reaction of mAbs with T. solium, E. multilocularis and E. vogeli antigen preparations in ELISA or IEP would suggest that these mAbs may recognise epitopes different from those of T. solium, E. multilocularis and E. vogeli parasites; this might be exploited for specific differentiation of E. granulosus.
journal_name
Parasitol Resjournal_title
Parasitology researchauthors
Liu D,Rickard MD,Lightowlers MWdoi
10.1007/BF00931225subject
Has Abstractpub_date
1993-01-01 00:00:00pages
82-5issue
1eissn
0932-0113issn
1432-1955journal_volume
79pub_type
杂志文章abstract：:Severe and complicated malaria is usually caused by Plasmodium falciparum malaria (PF) but it has been increasingly observed that Plasmodium vivax malaria (PV), which was otherwise considered to be benign malaria, with a low case-fatality ratio, can also occasionally result in severe disease as with PF malaria. There ...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-011-2776-7
更新日期：2012-06-01 00:00:00
abstract：:With the aim of proposing an alternative model to animal experimentation, we investigated cytokine production in response to antigens in an in vitro system. This is a co-culture system of healthy human leukocytes and enterocyte-like Caco-2 cells. The antigens tested, EgA31, EgTrp, and FABP1, are candidates for vaccine...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-004-1075-y
更新日期：2004-04-01 00:00:00
abstract：:Over a 4-year period, we analyzed 128 fecal samples from free-living elk (Alces alces L., 1758) to determine the prevalence of Eimeria infections and identify the species present. Two eimerian species were isolated including Eimeria alces and a morphotype resembling Eimeria catubrina. Overall, two samples from 128 sam...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-012-3262-6
更新日期：2013-05-01 00:00:00
abstract：:Exsheathment and midgut invasion of nocturnally subperiodic Brugia malayi microfilariae were analyzed using light and scanning electron microscopy in a refractory vector, Aedes aegypti (Thailand strain). Results showed that exsheathed microfilariae represented only approximately 1% of the total microfilaria midguts di...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-014-4086-3
更新日期：2014-11-01 00:00:00
abstract：:Thus far, Entamoeba species have been classified based on morphology such as the number of nuclei in mature cysts and their hosts. Using recently developed molecular tools, ruminant Entamoeba spp. are currently classified into four species/genotypes: E. bovis and Entamoeba ribosomal lineages (RL) 1, 2, and 4. However,...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-017-5689-2
更新日期：2018-01-01 00:00:00
abstract：:The migratory response of peripheral blood granulocytes and monocytes from the European eel Anguilla anguilla (L.) to infective larvae of the swimbladder nematode Anguillicola crassus Kuwahara, Niimi and Hagaki, 1974 was examined by means of light microscopical histology and with an in vitro assay using a modified Boy...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-008-0910-y
更新日期：2008-05-01 00:00:00
abstract：:Tetramicra brevifilum, a microsporidian parasite of Scophthalmus maximus, was found in Lophius budegassa for the first time. This parasite was detected in 5 of 199 hosts captured in the coastal waters of Barcelona (Northwest Mediterranean), which enlarges the geographic distribution of this microsporidian. Affected fi...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s004360050384
更新日期：1998-01-01 00:00:00
abstract：:Malaria is caused by unicellular parasites of the genus Plasmodium, which reside in erythrocytes during the clinically relevant stage of infection. To separate parasite from host cell material, haemolytic agents such as saponin are widely used. Previous electron microscopy studies on saponin-treated parasites reported...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-020-06932-9
更新日期：2020-12-01 00:00:00
abstract：:The hypothesis of a positive correlation between host specificity and taxonomic diversification was tested in a family of fish ectoparasites, the Diplectanidae Bychowsky 1957 (Monogenea). A comparative analysis of correlation of species richness with host specificity was performed using an adapted independent contrast...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s004360000325
更新日期：2001-03-01 00:00:00
abstract：:Toxoplasma gondii uses a unique mechanism to fulfill its asexual life cycles by which the parasite can infect all the warm-blooded animals including humans. Mitogen-activated protein kinase (MAPK) or extracellular signal-regulated kinase (ERK) pathway widely existed in eukaryotic cells mediates the conversion of envir...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-016-5103-5
更新日期：2016-09-01 00:00:00
abstract：:The prevalence data of Leishmania infantum infection in cats are characterized by a large variability mainly attributed to the differences in diagnostic techniques. In the absence of consensus about the method of choice for diagnosing feline leishmaniosis, the performance of a new immunofluorescence antibody test (IFA...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-020-06627-1
更新日期：2020-04-01 00:00:00
abstract：:Catecholamines, such as dopamine and noradrenaline, play important roles as neuromuscular transmitters and modulators in all parasitic helminthes, including Schistosoma japonicum. S. japonicum tyrosine hydroxylase (SjTH) was amplified by rapid amplification of cDNA ends polymerase chain reaction that shows strong homo...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-011-2347-y
更新日期：2011-10-01 00:00:00
abstract：:Tachyzoites of Toxoplasma gondii attach to the macrophage surface and are internalized either by a phagocytic process, which can be inhibited by cytochalasin D, or by an active process, independent of host cell actin. Previous studies have shown that parasite attachment induces the secretion of macromolecules found in...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/pl00008500
更新日期：2000-01-01 00:00:00
abstract：:Ras are key components of diverse signal transduction pathways and play important roles in growth and development. To know about growth regulation in Clonorchis sinensis, we have identified a full-length sequence encoding a ras-related protein (rap2) from our adult cDNA library. The open reading frame contains 561 bp ...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-010-2147-9
更新日期：2011-04-01 00:00:00
abstract：:Immunoperoxidase localisation of antigen at the electron microscope level confirms that parasite surface proteins, in association with membrane, are shed from the surface of the zoite on invasion, while varying amounts are also internalised. SAG 1 is stable on intracellular zoites for up to 48 h, although new protein ...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/BF00931842
更新日期：1995-01-01 00:00:00
abstract：:The beet cyst nematode Heterodera schachtii is an important pathogen worldwide, but its molecular characterization has been limited to studying individual genes of interest. We undertook a high-throughput genomic approach and drastically increased the number of available sequences for this parasite. A total of 2,662 e...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-005-0029-3
更新日期：2006-04-01 00:00:00
abstract：:The aim of this work was to perform an analysis based on mtDNA sequences of the 16S rRNA gene in order to determine the phylogenetic position of ticks belonging to the Rhipicephalus sanguineus group from the Canary Islands, Cyprus, and Croatia. All the haplotypes obtained from ticks collected in the Canary Islands and...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-019-06214-z
更新日期：2019-03-01 00:00:00
abstract：:Although cystic echinococcosis (CE) has been a recognized public health problem in Greece, molecular data are lacking regarding the types and prevalences of infecting strains of the etiological agent Echinococcus granulosus. Therefore, we investigated the prevalence of CE and determined the infecting genotypes in shee...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-007-0568-x
更新日期：2007-09-01 00:00:00
abstract：:To determine the prevalence of endoparasites and their association with diarrhea, a survey was conducted in the Southern Province of Zambia that used conventional and molecular techniques applied to stool and urine samples from school-age children (n = 93). Almost half of the stools (49.5%) were diarrhetic. The overal...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-005-0003-0
更新日期：2005-12-01 00:00:00
abstract：:Analysis of the initial interaction between Entamoeba histolytica trophozoites and the large intestine is impossible in humans and very difficult in experimental animals. To circumvent this obstacle we treated the luminal side of full-thickness rabbit colon segments mounted in Ussing-type chambers with trophozoite lys...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/BF00931595
更新日期：1993-01-01 00:00:00
abstract：:The relationships between inflammation in organs with Toxocara canis larval migration and matrix metalloproteinase-9 (MMP-9) were investigated following the infection of mice with 1,000 infective eggs. Gelatinase activity was defined by gelatin zymography, optimum pH, inhibitor specificity and Western blot analysis. M...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-004-1271-9
更新日期：2005-02-01 00:00:00
abstract：:The efficacies of 20 mg/kg body weight (BW) of toltrazuril (Toltra) 2 days post-infection (dpi), 2 mg/kg BW of diclazuril 2 and 3 dpi and 200 mg/kg BW of sulphadimidine 2, 3 and 4 dpi were compared in a model for piglet isosporosis. Weight gain (first 4 weeks of life) and diarrhoea and oocyst excretion from 4 to 11 dp...
journal_title：Parasitology research
pub_type： 杂志文章,随机对照试验
doi：10.1007/s00436-006-0314-9
更新日期：2007-01-01 00:00:00
abstract：:Investigations on the occurrence of trichodinid ciliates from fish caught in the Kiel Bight and Kiel Fjord (western Baltic Sea) were carried out between September 1996 and March 1997. Smears of the gills, fins, and skin of 120 Gadus morhua and 92 Platichthys flesus caught by fish traps and trammel nets revealed the pr...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s004360050622
更新日期：1999-08-01 00:00:00
abstract：:We have investigated how knowledge of endoplasmic reticulum (ER) retrieval signals can be used to study specific trafficking pathways in the malaria-infected erythrocyte. We show that addition of various lumenal ER retrieval signals to soluble green fluorescent protein (GFP) chimaera causes retrieval of the fusion pro...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-009-1397-x
更新日期：2009-06-01 00:00:00
abstract：:The diversity of ticks and tick-borne pathogens (TBPs) infesting domestic animals in Tchicala-Tcholoanga, Angola, in 2016 was investigated. Seventeen tick species were recorded, Amblyomma pomposum being the most abundant on cattle (40%), goats (38%) and sheep (35%); Rhipicephalus turanicus was the most abundant on dog...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-020-07009-3
更新日期：2021-01-08 00:00:00
abstract：:During the present investigation, alterations in the intrahepatic venous and portal systems in Rattus norvegicus infected with 250, 500, and 1000 Taenia taeniaeformis eggs were studied. The corrosion cast technique was employed, which provides an extrinsic opportunity for study of the three-dimensional orientation of ...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s004360050703
更新日期：2000-06-01 00:00:00
abstract：:The taxonomic status of the two known strains of Schistosoma intercalatum, the Lower Guinea strain (originating from Edea, Cameroon) and the Zaire strain (originating from Kinshasa, Democratic Republic of Congo, formerly Zaire) was examined using random amplified polymorphic DNA (RAPD) markers. Two additional species ...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s004360000301
更新日期：2001-01-01 00:00:00
abstract：:We examine the taxonomic composition and endemism of adult helminth parasites of freshwater fishes of Mexico, with regard to the main hydrological basins of the country. A presence-absence matrix, including every species of adult helminth parasites of freshwater fishes from 23 Mexican hydrological basins was compiled ...
journal_title：Parasitology research
pub_type： 杂志文章,评审
doi：10.1007/s00436-012-3175-4
更新日期：2013-01-01 00:00:00
abstract：:Climate change with an increase in average temperature may be responsible for propagation of temperature-dependent vectors and/or vector-associated pathogens in regions that were previously not endemic. The analysis of climate data of Brandenburg state (Germany) had showed that the extrinsic development of Dirofilaria...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-013-3431-2
更新日期：2013-07-01 00:00:00
abstract：:Spawning individuals of allis shad, Alosa alosa (Linnaeus, 1758), and twaite shad, Alosa fallax (Lacépède, 1803), were sampled from three rivers on the Atlantic coast of the Iberian Peninsula (Ulla, Minho, Mondego) during 2008 to 2013 to assess the presence of the zoonotic marine parasite Anisakis spp. larvae. The res...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-015-4403-5
更新日期：2015-06-01 00:00:00
