Abstract：
:The effects of trypsin, bile, trypsin-bile, pepsin, dithiothreitol (DTT) and metacercarial excretory-secretory product (ESP) on the in vitro excystment of Clonorchis sinensis metacercariae were investigated. The majority of metacercariae excysted immediately in trypsin-bile in PBS solution, a process which was complete after 30 min of incubation. When incubated in metacercarial ESP in PBS, excystment was potentiated in the presence of 5 mM DTT, but was inhibited dose-dependently by a cysteine protease inhibitor, iodoacetic acid. Two active protease bands of 28 and 40 kDa were identified in the ESP of metacercariae by gelatin substrate SDS-PAGE. Scanning electron microscopy demonstrated that the larvae in solutions of DTT and ESP migrated through a small hole on the metacercarial wall, whereas larvae were liberated by entire wall disruption in trypsin solution. These results suggest that trypsin is a major extrinsic factor of the rapid excystment of C. sinensis metacercariae, and that endogenous cysteine proteases are also involved in metacercarial excystment.
journal_name
Parasitol Resjournal_title
Parasitology researchauthors
Li S,Chung YB,Chung BS,Choi MH,Yu JR,Hong STdoi
10.1007/s00436-004-1097-5subject
Has Abstractpub_date
2004-05-01 00:00:00pages
36-40issue
1eissn
0932-0113issn
1432-1955journal_volume
93pub_type
杂志文章abstract：:Free living amoebae (FLA) are protists ubiquitously present in the environment. Aspergillus fumigatus is a mould responsible for severe deep-seated infections, and that can be recovered in the same habitats as the FLA. By conducting coculture experiments and fungal incubation with amoebal supernatants, we report herei...
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journal_title：Parasitology research
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pub_type： 杂志文章
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pub_type： 杂志文章
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journal_title：Parasitology research
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