Entamoeba histolytica sequences and their relationship with experimental liver abscesses in hamsters.

Abstract:

:The purpose of the present paper was to analyse the association between sequences of Entamoeba histolytica and their relationship with the development of hepatic abscesses in hamsters, using a complementary DNA library for E. histolytica. From the sequences obtained, we designed oligonucleotides for amplification by PCR. Trophozoites were isolated from faeces of 11 patients in whom cysts from E. histolytica were identified, and these trophozoites were then subjected to monoaxenic culture. Then 1 x 10(5) trophozoites were inoculated into hamster liver, with three hamsters used for every culture. Sequences were obtained for ubiquitin, lectin surface precursor, replication factor MCM3 and surface antigen. The associations between sequences and hepatic abscesses were: 11/11 for ubiquitin, 9/11 for lectin precursor, 4/11 for replication factor and 1/11 for surface antigen. These results suggest that ubiquitin could be an important protein involved in the mechanism of E. histolytica invasion.

journal_name

Parasitol Res

journal_title

Parasitology research

authors

Crisóstomo-Vázquez Mdel P,Jiménez-Cardoso E,Arroyave-Hernández C

doi

10.1007/s00436-005-0006-x

subject

Has Abstract

pub_date

2006-01-01 00:00:00

pages

94-8

issue

2

eissn

0932-0113

issn

1432-1955

journal_volume

98

pub_type

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