Abstract:
:The purpose of the present paper was to analyse the association between sequences of Entamoeba histolytica and their relationship with the development of hepatic abscesses in hamsters, using a complementary DNA library for E. histolytica. From the sequences obtained, we designed oligonucleotides for amplification by PCR. Trophozoites were isolated from faeces of 11 patients in whom cysts from E. histolytica were identified, and these trophozoites were then subjected to monoaxenic culture. Then 1 x 10(5) trophozoites were inoculated into hamster liver, with three hamsters used for every culture. Sequences were obtained for ubiquitin, lectin surface precursor, replication factor MCM3 and surface antigen. The associations between sequences and hepatic abscesses were: 11/11 for ubiquitin, 9/11 for lectin precursor, 4/11 for replication factor and 1/11 for surface antigen. These results suggest that ubiquitin could be an important protein involved in the mechanism of E. histolytica invasion.
journal_name
Parasitol Resjournal_title
Parasitology researchauthors
Crisóstomo-Vázquez Mdel P,Jiménez-Cardoso E,Arroyave-Hernández Cdoi
10.1007/s00436-005-0006-xsubject
Has Abstractpub_date
2006-01-01 00:00:00pages
94-8issue
2eissn
0932-0113issn
1432-1955journal_volume
98pub_type
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