Abstract：
:Hookworm infection is still a public health problem in developing countries. Aspartic protease plays an important role in parasite invasion and migration in the host. Aspartic protease gene from Ancylostoma caninum has been reported (Biochem Biophys Res Commun 227:294-302, 1996), but the activity of Acasp in eggs and larvae stage has not been studied. This paper reported the cloning of Acasp, expression in Escherichia coli, and characterization in eggs and larval stage. The mRNA encoding Acasp was detected using reverse transcriptase polymerase chain reaction in L4 and adult worm. A polyclonal antiserum against E. coli expressed recombinant Acasp was produced and used to detect and localize the Acasp in various developmental stages of A. caninum. Results from Western blot and indirect fluorescent immunoassay showed that the Acasp was present in the embryo, larvae, as well as in the adult worms. The recombinant Acasp exhibited the protease activity by the gelatin gel digestion assay.
journal_name
Parasitol Resjournal_title
Parasitology researchauthors
Yang Y,Wei H,Qin W,Zheng Jdoi
10.1007/s00436-009-1332-1subject
Has Abstractpub_date
2009-06-01 00:00:00pages
1327-33issue
6eissn
0932-0113issn
1432-1955journal_volume
104pub_type
杂志文章abstract：:Dengue is an arthropod-borne viral infection mainly vectored through the bite of Aedes mosquitoes. Recently, its transmission has strongly increased in urban and semi-urban areas of tropical and sub-tropical regions worldwide, becoming a major international public health concern. There is no specific treatment for den...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-015-4556-2
更新日期：2015-09-01 00:00:00
abstract：:Two soluble Toxoplasma gondii antigen preparations were compared using the immunoblotting technique. The first preparation, which is commonly employed in ELISA tests, corresponds to a lytic extract of the parasite. The second known as exo-antigen, has yielded good results in cell immunity research and is obtained from...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/BF00931730
更新日期：1992-01-01 00:00:00
abstract：:Fecal specimens from 432 pre-weaned calves younger than 35 days were collected over a 2-year period (2010-2012) from 74 dairy cattle farms in the central area of Colombia. These samples were microscopically examined for the presence of Cryptosporidium oocysts, and positive specimens were selected for molecular examina...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-018-5818-6
更新日期：2018-05-01 00:00:00
abstract：:Angiostrongylus costaricensis can infect several mollusks, and its migration route in intermediate hosts has been studied only in Sarasinula marginata. To verify the susceptibility of Omalonyx sp. as an intermediate host of A. costaricensis and to analyze the nematode migration route, individuals were infected with st...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-008-1138-6
更新日期：2008-11-01 00:00:00
abstract：:The prevalence data of Leishmania infantum infection in cats are characterized by a large variability mainly attributed to the differences in diagnostic techniques. In the absence of consensus about the method of choice for diagnosing feline leishmaniosis, the performance of a new immunofluorescence antibody test (IFA...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-020-06627-1
更新日期：2020-04-01 00:00:00
abstract：:The parasite community structure of the neotropical cormorant, Phalacrocorax brasilianus, from two lagoons (Coyuca and Tres Palos) from Guerrero state, México, was examined. Fourteen species of adult helminths (6,391 individuals) from 48 cormorants were identified: 9 digeneans, 1 acanthocephalan, 1 cestode, and 3 nema...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-011-2377-5
更新日期：2011-11-01 00:00:00
abstract：:The objectives of this study were to evaluate the occurrence of Leishmania infantum in the male and female genital tract and female mammary glands of dogs and the parasite burden and to identify histological alterations associated with this protozoan. Twenty male and 20 female Leishmania-seropositive dogs with isolati...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-016-4987-4
更新日期：2016-06-01 00:00:00
abstract：:Climate change with an increase in average temperature may be responsible for propagation of temperature-dependent vectors and/or vector-associated pathogens in regions that were previously not endemic. The analysis of climate data of Brandenburg state (Germany) had showed that the extrinsic development of Dirofilaria...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-013-3431-2
更新日期：2013-07-01 00:00:00
abstract：:The anthelmintic potentials of the chloroform and methanol extracts of Buchholzia coriacea Engler seed were investigated. In folklore medicine, B. coriacea (Capparidaceae) is believed to be useful in the treatment of various kinds of ailments and diseases. At doses of 10 mg/kg, 25 mg/kg and 50 mg/kg, the extracts were...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-011-2274-y
更新日期：2011-08-01 00:00:00
abstract：:The genomic DNA was extracted from nematode parasites, Heterakis gallinae from specific host, native chickens. The polymerase chain reaction (PCR) was applied to make amplification of the DNA content that aids in the further studies. The primer was used in the PCR reactions that have the following sequences: forward (...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-013-3539-4
更新日期：2013-10-01 00:00:00
abstract：:The fine structure of the infective hexacanths of Echinococcus multilocularis was examined with particular emphasis on the functional ultrastructure of penetration glands and nerve cells directly involved in the mechanism of initial host infection. The oncosphere contains two types of penetration glands, PG1 and PG2, ...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-018-5957-9
更新日期：2018-08-01 00:00:00
abstract：:This study was conducted to evaluate the phytochemical composition and larvicidal effect of leaf essential oil from Murraya exotica against early fourth-instar larvae of Aedes aegypti, Anopheles stephensi and Culex quinquefasciatus. Gas chromatography (GC) and gas chromatography mass spectrometry (GC-MS) analyses reve...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-015-4370-x
更新日期：2015-05-01 00:00:00
abstract：:Many factors contribute to parasites varying in host specificity and distribution among potential hosts. Metagonimoides oregonensis is a digenetic trematode that uses stream-dwelling plethodontid salamanders as second intermediate hosts in the Eastern US. We completed a field survey to identify which stream salamander...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-015-4550-8
更新日期：2015-08-01 00:00:00
abstract：:Echinostoma trivolvis adults grown in golden hamsters were treated in vitro with [3H]-tyrosine to label sperm and transplanted to uninfected hamsters alone or with four to six unlabeled adults. After 4-5 days, worms were recovered, processed for autoradiography, and observed for silver grains over their seminal recept...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/BF00932258
更新日期：1993-01-01 00:00:00
abstract：:Filter-feeding molluscan shellfish can concentrate environmentally derived waterborne pathogens of humans, which can be utilized in the sanitary assessment of water quality. In the present study, oocysts of Cryptosporidium were detected in Bent mussels (Ischadium recurvum) at two Chesapeake Bay sites from which C. par...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s004360050590
更新日期：1999-07-01 00:00:00
abstract：:The oocysts of Caryospora cherrughi sp. n. were described from the feces of the saker falcon, Falco cherrug, collected from the falcon market in Riyadh City, Saudi Arabia. The prevalence of infection was 21.4 % (15/70). The majority of the oocysts examined had completed sporulation within 64-84 h at 24 ± 2 °C. Sporula...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-012-3217-y
更新日期：2013-03-01 00:00:00
abstract：:A void volume fraction and fractions of mean sizes 800 kdalton and 300 kdalton were isolated by gel filtration from lysate of bovine erythrocytes infected with Babesia bovis. All fractions had good serological activity, as assayed by ELISA and IFA. Groups of four splenectomized calves were vaccinated with each fractio...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/BF00531085
更新日期：1987-01-01 00:00:00
abstract：:The present report demonstrates that the Gymnorhynchus gigas plerocercoid possesses various types of endo- and exoproteases with activity against general (azocoll, azocasein, and azoalbumin) and specific substrates (elastin, keratin, collagen, hemoglobin, fibrinogen, plasma, and immunoglobulin G). The activity against...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s004360050508
更新日期：1999-01-01 00:00:00
abstract：:The morphology and ecology are described for four sibling species of digenean metacercariae: Meiogymnophallus minutus (Cobbold 1859), M. strigatus (Lebour 1908) from the estuarine cockle Cerastoderma edule L. and M. fossarum (Bartoli, 1965) and M. rebecqui (Bartoli, 1983) comb. nov. from the brackish water lagoon cock...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s004360050153
更新日期：1996-01-01 00:00:00
abstract：:A case of orotracheal myiasis in an 80-year-old man in coma for 1 week in the intensive care unit of Baskent University Hospital, Ankara, Turkey, is reported. A number of larvae of Wohlfahrtia magnifica were recovered repeatedly from the mouth and from the intubation tube of the patient and were reared to adults to fa...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s004360050203
更新日期：1997-01-01 00:00:00
abstract：:Distinct sequences of Giardia duodenalis assemblages raised the hypothesis that certain assemblages may contribute to its clinical outcome. However, sequences analysis is time consuming, expensive, and needs many manual operations. Nested PCR targeting intergenic spacer (IGS) region was applied successfully to genotyp...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-016-5347-0
更新日期：2017-02-01 00:00:00
abstract：:An overall 44% transplacental transmission rate was observed in 221 rats fed cysts of 12 Toxoplasma strains at 15 days of pregnancy, with a range of 0-90% transmission. Considerable variability in the transmission rate was seen among different groups of rats that received similar Toxoplasma inocula; this is attributed...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s004360100486
更新日期：2001-11-01 00:00:00
abstract：:Aelurostrongylus abstrusus parasitizes the respiratory tract and can heavily affect the breathing and general condition of cats. Experimental infections of six cats were initiated by intragastric administration with 100 or 800 third-stage larvae (L3) obtained from the terrestrial snail Helix aspersa. First-stage larva...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-014-3783-2
更新日期：2014-04-01 00:00:00
abstract：:Seventy specimens of Hypsiboas albopunctatus (Anura: Hylidae) were collected and surveyed for endoparasites in Brasília, Brazil at the end of the rainy season (February and March) in 2005. Sixteen males (22.86%) were infected by Rhabdias sp., Aplectana sp., Cosmocerca sp., and Falcaustra mascula. Rhabdias sp., a lung ...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-007-0797-z
更新日期：2008-03-01 00:00:00
abstract：:The spatial distribution of parasite communities from teleost fishes (Diplodus vulgaris, Mullus surmuletus, Pagellus erythrinus, Phycis phycis, Scorpaena scrofa and Symphodus tinca) was studied in the Bonifacio Strait Marine Reserve (Corsica Island) with different protection levels. Canonical correspondence analysis w...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-008-1323-7
更新日期：2009-06-01 00:00:00
abstract：:We have previously reported the involvement of cyclic AMP in regulating flagellar waveforms in Leishmania. Here, we investigated the roles of calcium, calmodulin, and calcineurin in flagellar motility regulation in L. donovani. Using high-speed videomicroscopy, we show that calcium-independent calmodulin and calcineur...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-017-5608-6
更新日期：2017-11-01 00:00:00
abstract：:Ticks and tick-borne diseases (TBDs) continue to pose an insidious and ever-present threat to livestock and livelihoods across the globe. Two of the most significant TBDs of cattle in Africa are heartwater and babesioisis, caused by Ehrlichia ruminantium and Babesia bigemina respectively. Both pathogens are endemic in...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-019-06204-1
更新日期：2019-03-01 00:00:00
abstract：:Gas-liquid chromatographic studies were done to determine the fatty acid composition of the digestive gland-gonad (DGG) complex of Helisoma trivolvis snails infected with the intramolluscan stages of Echinostoma trivolvis, of rediae freed from the DGG, of uninfected DGG, and of 41-day-old adult worms grown in golden h...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/BF00931585
更新日期：1993-01-01 00:00:00
abstract：:We analyzed extra chromosomal genomes of avian blood protozoa, Leucocytozoon caulleryi. One of the genomes, the mitochondrial genome was completely sequenced resulting 5,959 bp in length. This genome contained the identical gene organization and contents to that of other avian blood protozoa previously analyzed: three...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-008-1083-4
更新日期：2008-09-01 00:00:00
abstract：:Haemosporidian blood parasites of the Plasmodium genus are the causative agents of avian malaria in many parts of the world. Despite the great diversity of Brazilian avifauna, few studies have been conducted to examine the haemosporidians of wild birds found in the Brazilian Atlantic Forest, especially those kept in c...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-017-5465-3
更新日期：2017-07-01 00:00:00
