Abstract:
:Trichomonas gallinae are parasitic flagellates of importance in wild and domestic birds. The parasite is worldwide distributed, and Columbine birds are its main host. Current research focuses mostly on epidemiological and phylogenetic studies. However, there is still a lack of knowledge regarding parasite-host interaction or therapy development. Real-time PCR is a useful tool for diagnostic and quantification of gene copies in a determined sample. By amplification of a 113-bp region of the 18S small subunit ribosomal RNA gene, a SYBR green-based real-time PCR assay was developed. A standard curve was prepared for quantification analysis. Assay efficiency, linearity, and dissociation analysis were successfully performed. Specificity, sensibility, and reproducibility analysis were tested. This assay could be a useful tool not only for diagnostic purposes but also for future in vivo and in vitro T. gallinae studies.
journal_name
Parasitol Resjournal_title
Parasitology researchauthors
Rentería-Solís Z,Nguyen-Ho-Bao T,Taha S,Daugschies Adoi
10.1007/s00436-020-06887-xsubject
Has Abstractpub_date
2020-11-01 00:00:00pages
3909-3913issue
11eissn
0932-0113issn
1432-1955pii
10.1007/s00436-020-06887-xjournal_volume
119pub_type
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