A SYBR green I real-time polymerase chain reaction (PCR) assay for detection and quantification of Trichomonas gallinae.

Abstract:

:Trichomonas gallinae are parasitic flagellates of importance in wild and domestic birds. The parasite is worldwide distributed, and Columbine birds are its main host. Current research focuses mostly on epidemiological and phylogenetic studies. However, there is still a lack of knowledge regarding parasite-host interaction or therapy development. Real-time PCR is a useful tool for diagnostic and quantification of gene copies in a determined sample. By amplification of a 113-bp region of the 18S small subunit ribosomal RNA gene, a SYBR green-based real-time PCR assay was developed. A standard curve was prepared for quantification analysis. Assay efficiency, linearity, and dissociation analysis were successfully performed. Specificity, sensibility, and reproducibility analysis were tested. This assay could be a useful tool not only for diagnostic purposes but also for future in vivo and in vitro T. gallinae studies.

journal_name

Parasitol Res

journal_title

Parasitology research

authors

Rentería-Solís Z,Nguyen-Ho-Bao T,Taha S,Daugschies A

doi

10.1007/s00436-020-06887-x

subject

Has Abstract

pub_date

2020-11-01 00:00:00

pages

3909-3913

issue

11

eissn

0932-0113

issn

1432-1955

pii

10.1007/s00436-020-06887-x

journal_volume

119

pub_type

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