Abstract:
:Recombinant adeno-associated virus (AAV) vectors are powerful tools for both basic neuroscience experiments and clinical gene therapies for neurological diseases. Intravascularly administered self-complementary AAV9 vectors can cross the blood-brain barrier. However, AAV9 vectors are of limited usefulness because they mainly transduce astrocytes in adult animal brains and have restrictions on foreign DNA package sizes. In this study, we show that intracardiac injections of tyrosine-mutant pseudotype AAV9/3 vectors resulted in extensive and widespread transgene expression in the brains and spinal cords of adult mice. Furthermore, the usage of neuron-specific promoters achieved selective transduction of neurons. These results suggest that tyrosine-mutant AAV9/3 vectors may be effective vehicles for delivery of therapeutic genes, including miRNAs, into the brain and for treating diseases that affect broad areas of the central nervous system.
journal_name
Biomed Res Intjournal_title
BioMed research internationalauthors
Iida A,Takino N,Miyauchi H,Shimazaki K,Muramatsu Sdoi
10.1155/2013/974819subject
Has Abstractpub_date
2013-01-01 00:00:00pages
974819eissn
2314-6133issn
2314-6141journal_volume
2013pub_type
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journal_title:BioMed research international
pub_type: 杂志文章
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更新日期:2013-01-01 00:00:00
abstract:PURPOSE:To determine the predictive factors that affect short term and long term postoperative drift in intermittent exotropia after bilateral lateral rectus recession and to evaluate its effect on surgical outcome. METHODS:Retrospective review of 203 patients with diagnosis of intermittent exotropia, who had surgical...
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journal_title:BioMed research international
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journal_title:BioMed research international
pub_type: 杂志文章,评审
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doi:10.1155/2017/6069374
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pub_type: 临床试验,杂志文章
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pub_type: 杂志文章,评审
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journal_title:BioMed research international
pub_type: 杂志文章
doi:10.1155/2016/5473204
更新日期:2016-01-01 00:00:00
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pub_type: 临床试验,杂志文章
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