Abstract:
:The release of ATP from the urothelium in response to stretch during filling demonstrates the importance of the purinergic system for the physiological functioning of the bladder. This study examined the effect of P2 receptor agonists on ATP release from two urothelial cell lines (RT4 and UROtsa cells). Hypotonic Krebs was used as a stretch stimulus. Incubation of urothelial cells with high concentrations of the P2Y agonist ADP induced ATP release to a level that was 40-fold greater than hypotonic-stimulated ATP release (P < 0.0011, ADP EC50 1.8 µM). Similarly, an increase in ATP release was also observed with the P2Y agonist, UTP, up to a maximum of 70% of the hypotonic response (EC50 0.62 µM). Selective P2 receptor agonists, αβ -methylene-ATP, ATP- γ -S, and 2-methylthio-ADP had minimal effects on ATP release. ADP-stimulated ATP release was significantly inhibited by suramin (100 µM, P = 0.002). RT4 urothelial cells break down nucleotides (100 µM) including ATP, ADP, and UTP to liberate phosphate. Phosphate liberation was also demonstrated from endogenous nucleotides with approximately 10% of the released ATP broken down during the incubation. These studies demonstrate a role for P2Y receptor activation in stimulation of ATP release and emphasize the complexity of urothelial P2 receptor signalling.
journal_name
Biomed Res Intjournal_title
BioMed research internationalauthors
Mansfield KJ,Hughes JRdoi
10.1155/2014/830374subject
Has Abstractpub_date
2014-01-01 00:00:00pages
830374eissn
2314-6133issn
2314-6141journal_volume
2014pub_type
杂志文章abstract::Activity-dependent plasticity as a result of reorganization of neural circuits is a fundamental characteristic of the central nervous system that occurs simultaneously in multiple sites. In this study, we established the effects of subthreshold transcranial magnetic stimulation (TMS) over the primary motor cortex regi...
journal_title:BioMed research international
pub_type: 临床试验,杂志文章
doi:10.1155/2013/516427
更新日期:2013-01-01 00:00:00
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pub_type: 杂志文章
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更新日期:2017-01-01 00:00:00
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journal_title:BioMed research international
pub_type: 临床试验,杂志文章
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journal_title:BioMed research international
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journal_title:BioMed research international
pub_type: 杂志文章
doi:10.1155/2016/4182595
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journal_title:BioMed research international
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doi:10.1155/2014/218546
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doi:10.1155/2019/4370382
更新日期:2019-10-07 00:00:00
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pub_type: 杂志文章
doi:10.1155/2018/3061742
更新日期:2018-11-01 00:00:00
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journal_title:BioMed research international
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doi:10.1155/2016/6451849
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journal_title:BioMed research international
pub_type: 临床试验,杂志文章
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journal_title:BioMed research international
pub_type: 杂志文章
doi:10.1155/2015/435253
更新日期:2015-01-01 00:00:00
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journal_title:BioMed research international
pub_type: 杂志文章
doi:10.1155/2016/7529521
更新日期:2016-01-01 00:00:00
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journal_title:BioMed research international
pub_type: 杂志文章,评审
doi:10.1155/2013/849819
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journal_title:BioMed research international
pub_type: 杂志文章,评审
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journal_title:BioMed research international
pub_type: 临床试验,杂志文章
doi:10.1155/2015/730291
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pub_type: 杂志文章
doi:10.1155/2015/328628
更新日期:2015-01-01 00:00:00
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journal_title:BioMed research international
pub_type: 杂志文章
doi:10.1155/2020/5315930
更新日期:2020-10-05 00:00:00
abstract::Objectives. To construct a frailty index from next-of-kin information of the last year of life of community-dwelling 50 years old or older adults and test its association with health services utilization. Methods. Cross-sectional analysis from next-of-kin data available from the last wave of the Mexican Health and Agi...
journal_title:BioMed research international
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doi:10.1155/2017/6069374
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abstract::The interplay between Aspergillus fumigatus and the host immune response in lung infection has been subject of studies over the last years due to its importance in immunocompromised patients. The multifactorial virulence factors of A. fumigatus are related to the fungus biological characteristics, for example, structu...
journal_title:BioMed research international
pub_type: 杂志文章,评审
doi:10.1155/2013/693023
更新日期:2013-01-01 00:00:00
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journal_title:BioMed research international
pub_type: 杂志文章
doi:10.1155/2015/516984
更新日期:2015-01-01 00:00:00
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journal_title:BioMed research international
pub_type: 杂志文章,评审
doi:10.1155/2015/434683
更新日期:2015-01-01 00:00:00
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pub_type: 杂志文章,评审
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