Abstract:
:The large-scale production of cardiomyocytes is a key step in the development of cell therapy and tissue engineering to treat cardiovascular diseases, particularly those caused by ischemia. The main objective of this study was to establish a procedure for the efficient production of cardiomyocytes by reprogramming mesenchymal stem cells from adipose tissue. First, lentiviral vectors expressing neoR and GFP under the control of promoters expressed specifically during cardiomyogenesis were constructed to monitor cell reprogramming into precardiomyocytes and to select cells for amplification and characterization. Cellular reprogramming was performed using 5'-azacytidine followed by electroporation with plasmid pOKS2a, which expressed Oct4, Sox2, and Klf4. Under these conditions, GFP expression began only after transfection with pOKS2a, and less than 0.015% of cells were GFP(+). These GFP(+) cells were selected for G418 resistance to find molecular markers of cardiomyocytes by RT-PCR and immunocytochemistry. Both genetic and protein markers of cardiomyocytes were present in the selected cells, with some variations among them. Cell doubling time did not change after selection. Together, these results indicate that enrichment with vectors expressing GFP and neoR under cardiomyocyte-specific promoters can produce large numbers of cardiomyocyte precursors (CMPs), which can then be differentiated terminally for cell therapy and tissue engineering.
journal_name
Biomed Res Intjournal_title
BioMed research internationalauthors
Zanetti BF,Gomes WJ,Han SWdoi
10.1155/2013/390789subject
Has Abstractpub_date
2013-01-01 00:00:00pages
390789eissn
2314-6133issn
2314-6141journal_volume
2013pub_type
杂志文章abstract::The study aims to investigate the effects of the alcohol extract of Coreopsis tinctoria Nutt (AC) on diabetic nephropathy (DN) mice. A total of 30 db/db (DN) mice were divided into 3 groups, which were treated with AC (300 mg/kg/day), metformin (180 mg/kg/day), or saline by gavage for 10 weeks. Ten db/m mice treated w...
journal_title:BioMed research international
pub_type: 杂志文章
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journal_title:BioMed research international
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pub_type: 杂志文章,评审
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journal_title:BioMed research international
pub_type: 杂志文章
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journal_title:BioMed research international
pub_type: 杂志文章,评审
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更新日期:2013-01-01 00:00:00
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journal_title:BioMed research international
pub_type: 杂志文章,评审
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更新日期:2015-01-01 00:00:00
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journal_title:BioMed research international
pub_type: 杂志文章,评审
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journal_title:BioMed research international
pub_type: 杂志文章,meta分析
doi:10.1155/2019/2139834
更新日期:2019-11-26 00:00:00
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pub_type: 临床试验,杂志文章
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pub_type: 杂志文章,多中心研究
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pub_type: 杂志文章,评审
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pub_type: 杂志文章
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