当前位置： SCI文献检索 > Biomed Research International期刊下所有文献 > A pipeline with multiplex reverse transcription polymerase chain reaction and microarray for screening of chromosomal translocations in leukemia.

A pipeline with multiplex reverse transcription polymerase chain reaction and microarray for screening of chromosomal translocations in leukemia.

Abstract：

:Chromosome rearrangements and fusion genes present major portion of leukemogenesis and contribute to leukemic subtypes. It is practical and helpful to detect the fusion genes in clinic diagnosis of leukemia. Present application of reverse transcription polymerase chain reaction (RT-PCR) method to detect the fusion gene transcripts is effective, but time- and labor-consuming. To set up a simple and rapid system, we established a method that combined multiplex RT-PCR and microarray. We selected 15 clinically most frequently observed chromosomal rearrangements generating more than 50 fusion gene variants. Chimeric reverse primers and chimeric PCR primers containing both gene-specific and universal sequences were applied in the procedure of multiplex RT-PCR, and then the PCR products hybridized with a designed microarray. With this approach, among 200 clinic samples, 63 samples were detected to have gene rearrangements. All the detected fusion genes positive and negative were validated with RT-PCR and Sanger sequencing. Our data suggested that the RT-PCR-microarray pipeline could screen 15 partner gene pairs simultaneously at the same accuracy of the fusion gene detection with regular RT-PCR. The pipeline showed effectiveness in multiple fusion genes screening in clinic samples.

journal_name

Biomed Res Int

journal_title

BioMed research international

authors

Xiong FF,Li BS,Zhang CX,Xiong H,Shen SH,Zhang QH

doi

10.1155/2013/135086

subject

Has Abstract

pub_date

2013-01-01 00:00:00

pages

135086

eissn

2314-6133

issn

2314-6141

journal_volume

2013

pub_type

杂志文章

相关文献

Biomed Research International文献大全
  • Effects of Arteriovenous Thrombolysis Combined with Mechanical Thrombectomy on Efficacy and Neurological Function of Acute Cerebral Infarct Patients.

    abstract：Objective:To investigate the effects of arteriovenous thrombolysis combined with mechanical thrombectomy on clinical efficacy, neurological function, and the changes of nerve injury markers of acute cerebral infarct (ACI) patients. Methods:A total of 143 cases with ACI admitted to our hospital from June 2017 to June 2...

    journal_title：BioMed research international

    pub_type： 杂志文章

    doi：10.1155/2020/9743075

    authors： Ding T,Tang L,Hu B,Yuan J,Li X,Wen J

    更新日期：2020-10-09 00:00:00

  • Variants of SCARB1 and VDR Involved in Complex Genetic Interactions May Be Implicated in the Genetic Susceptibility to Clear Cell Renal Cell Carcinoma.

    abstract：:The current data are still inconclusive in terms of a genetic component involved in the susceptibility to renal cell carcinoma. Our aim was to evaluate 40 selected candidate polymorphisms for potential association with clear cell renal cell carcinoma (ccRCC) based on independent group of 167 patients and 200 healthy c...

    journal_title：BioMed research international

    pub_type： 临床试验,杂志文章

    doi：10.1155/2015/860405

    authors： Pośpiech E,Ligęza J,Wilk W,Gołas A,Jaszczyński J,Stelmach A,Ryś J,Blecharczyk A,Wojas-Pelc A,Jura J,Branicki W

    更新日期：2015-01-01 00:00:00

  • Possible Effect of Concomitant Prokinetics and Herbal Medicines against Nausea in Patients Taking Lubiprostone.

    abstract：Background and Aim:Lubiprostone is a novel laxative that sometimes causes nausea, but preventive strategies remain unconfirmed. Methods:We retrospectively chose 126 patients prescribed lubiprostone from 2013 to 2016. Medical records were reviewed to clarify whether nausea developed after administration of the drug. Ba...

    journal_title：BioMed research international

    pub_type： 杂志文章

    doi：10.1155/2017/3762179

    authors： Yamamoto T,Osumi S,Yanagisawa D,Yamato H,Aoyagi H,Isono A,Abe K,Kita H

    更新日期：2017-01-01 00:00:00

  • Enhanced Production of Polymyxin E in Paenibacillus polymyxa by Replacement of Glucose by Starch.

    abstract：:Polymyxin E or colistin, produced by Paenibacillus polymyxa, is an important antibiotic against Gram-negative pathogens. The objective of this study is to evaluate the effect of starch in fermentation medium on colistin biosynthesis in P. polymyxa. The results indicated that replacement of glucose by starch stimulated...

    journal_title：BioMed research international

    pub_type： 杂志文章

    doi：10.1155/2018/1934309

    authors： Yu Z,Sun Z,Yin J,Qiu J

    更新日期：2018-10-14 00:00:00

  • The Association between PON1 (Q192R and L55M) Gene Polymorphisms and Risk of Cancer: A Meta-Analysis Based on 43 Studies.

    abstract：:Q192R and L55M polymorphism were considered to be associated with the development of multiple cancers. Nevertheless, the results of these researches were inconclusive and controversial. Therefore, we conducted a meta-analysis of all eligible case-control studies to assess the association between PON1 (Q192R and L55M) ...

    journal_title：BioMed research international

    pub_type： 杂志文章,meta分析,评审

    doi：10.1155/2019/5897505

    authors： Pan X,Huang L,Li M,Mo D,Liang Y,Liu Z,Huang Z,Huang L,Liu J,Zhu B

    更新日期：2019-07-28 00:00:00

  • Autophagy Promoted the Degradation of Mutant ATXN3 in Neurally Differentiated Spinocerebellar Ataxia-3 Human Induced Pluripotent Stem Cells.

    abstract：:Spinocerebellar ataxia-3 (SCA3) is the most common dominant inherited ataxia worldwide and is caused by an unstable CAG trinucleotide expansion mutation within the ATXN3 gene, resulting in an expanded polyglutamine tract within the ATXN3 protein. Many in vitro studies have examined the role of autophagy in neurodegene...

    journal_title：BioMed research international

    pub_type： 杂志文章

    doi：10.1155/2016/6701793

    authors： Ou Z,Luo M,Niu X,Chen Y,Xie Y,He W,Song B,Xian Y,Fan D,OuYang S,Sun X

    更新日期：2016-01-01 00:00:00

  • 3D Culture of MIN-6 Cells on Decellularized Pancreatic Scaffold: In Vitro and In Vivo Study.

    abstract：:Type 1 diabetes is an autoimmune disease which is due to the lack of β cells. The ideal therapy to cure the disease is pancreas transplantation, but its application is confined to a limited number of people due to the shortage of organ and the need for life-long immunosuppression. Regenerative medicine methods such as...

    journal_title：BioMed research international

    pub_type： 杂志文章

    doi：10.1155/2015/432645

    authors： Wu D,Wan J,Huang Y,Guo Y,Xu T,Zhu M,Fan X,Zhu S,Ling C,Li X,Lu J,Zhu H,Zhou P,Lu Y,Wang Z

    更新日期：2015-01-01 00:00:00

  • Antioxidant and hepatoprotective effect of aqueous extract of germinated and fermented mung bean on ethanol-mediated liver damage.

    abstract：:Mung bean is a hepatoprotective agent in dietary supplements. Fermentation and germination processes are well recognized to enhance the nutritional values especially the concentration of active compounds such as amino acids and GABA of various foods. In this study, antioxidant and hepatoprotective effects of freeze-dr...

    journal_title：BioMed research international

    pub_type： 杂志文章

    doi：10.1155/2013/693613

    authors： Mohd Ali N,Mohd Yusof H,Long K,Yeap SK,Ho WY,Beh BK,Koh SP,Abdullah MP,Alitheen NB

    更新日期：2013-01-01 00:00:00

  • Contrast-Enhanced Mammography (CEM) for Detecting Residual Disease after Neoadjuvant Chemotherapy: A Comparison with Breast Magnetic Resonance Imaging (MRI).

    abstract：Objective:To evaluate the performance of contrast-enhanced mammography (CEM) compared to magnetic resonance imaging (MRI) for estimating residual tumor size after neoadjuvant chemotherapy (NAC) in women with newly diagnosed breast cancer. Methods:The institutional review board approved this study. This prospective stu...

    journal_title：BioMed research international

    pub_type： 杂志文章

    doi：10.1155/2018/8531916

    authors： Barra FR,Sobrinho AB,Barra RR,Magalhães MT,Aguiar LR,de Albuquerque GFL,Costa RP,Farage L,Pratesi R

    更新日期：2018-11-08 00:00:00

  • Whether Keratectasia Area Shown in Corneal Topography Is Appropriate for Evaluating the Effect of Corneal Cross-Linking for Keratoconus: A 12-Month Follow-Up Study.

    abstract：Purpose:To analyze the keratectasia area (KEA) shown in corneal topography before and after corneal cross-linking (CXL) in patients with progressive keratoconus (KC) and figure out whether KEA is appropriate for evaluating the effect of CXL. Methods:A retrospective analysis was conducted in 34 eyes from 24 progressive...

    journal_title：BioMed research international

    pub_type： 杂志文章

    doi：10.1155/2019/1762537

    authors： Wang J,Li Z,Zhang H,Gao N,Mu G

    更新日期：2019-04-03 00:00:00

  • Amplification of the insulin-like growth factor 1 receptor gene is a rare event in adrenocortical adenocarcinomas: searching for potential mechanisms of overexpression.

    abstract：CONTEXT:IGF1R overexpression appears to be a prognostic biomarker of metastatic pediatric adrenocortical tumors. However, the molecular mechanisms that are implicated in its upregulation remain unknown. Aim. To investigate the potential mechanisms involved in IGF1R overexpression. PATIENTS AND METHODS:We studied 64 ad...

    journal_title：BioMed research international

    pub_type： 临床试验,杂志文章

    doi：10.1155/2014/936031

    authors： Ribeiro TC,Jorge AA,Almeida MQ,Mariani BM,Nishi MY,Mendonca BB,Fragoso MC,Latronico AC

    更新日期：2014-01-01 00:00:00

  • Rare Mutations in AHDC1 in Patients with Obstructive Sleep Apnea.

    abstract：Objectives:Obstructive sleep apnea (OSA) is a common disorder influenced by genetic and environmental factors. Mutations of AT-hook DNA-binding motif containing 1 (AHDC1) gene have been implicated which could cause rare syndromes presenting OSA. This study aims to investigate some rare mutations of AHDC1 in Chinese Han...

    journal_title：BioMed research international

    pub_type： 杂志文章

    doi：10.1155/2019/5907361

    authors： Yang S,Li K,Zhu MM,Yuan XD,Jiao XL,Yang YY,Li J,Li L,Zhang HN,Du YH,Wei YX,Qin YW

    更新日期：2019-10-13 00:00:00

  • In Vivo Osseointegration Performance of Titanium Dioxide Coating Modified Polyetheretherketone Using Arc Ion Plating for Spinal Implant Application.

    abstract：:Polyetheretherketone (PEEK), which has biomechanical performance similar to that of human cancellous bone, is used widely as a spinal implant material. However, its bioinertness and hydrophobic surface properties result in poor osseointegration. This study applies a novel modification method, arc ion plating (AIP), th...

    journal_title：BioMed research international

    pub_type： 杂志文章

    doi：10.1155/2015/328943

    authors： Tsou HK,Chi MH,Hung YW,Chung CJ,He JL

    更新日期：2015-01-01 00:00:00

  • Corrigendum to "All-Atom Four-Body Knowledge-Based Statistical Potentials to Distinguish Native Protein Structures from Nonnative Folds".

    abstract：:[This corrects the article DOI: 10.1155/2017/5760612.]. ...

    journal_title：BioMed research international

    pub_type： 杂志文章,已发布勘误

    doi：10.1155/2018/7108272

    authors： Masso M

    更新日期：2018-01-08 00:00:00

  • Imaging and Management of Incidental Renal Lesions.

    abstract：:The increased use of imaging modalities in the last years has led to a greater incidence in depicting abdominal incidental lesions. In particular, "incidentalomas" of the kidney are discovered in asymptomatic patients or patients who suffer from diseases not directly related to the kidneys. The aim of this paper is to...

    journal_title：BioMed research international

    pub_type： 杂志文章,评审

    doi：10.1155/2017/1854027

    authors： Mazziotti S,Cicero G,D'Angelo T,Marino MA,Visalli C,Salamone I,Ascenti G,Blandino A

    更新日期：2017-01-01 00:00:00

  • Adipose tissue-derived stem cell secreted IGF-1 protects myoblasts from the negative effect of myostatin.

    abstract：:Myostatin, a TGF-β family member, is associated with inhibition of muscle growth and differentiation and might interact with the IGF-1 signaling pathway. Since IGF-1 is secreted at a bioactive level by adipose tissue-derived mesenchymal stem cells (ASCs), these cells (ASCs) provide a therapeutic option for Duchenne Mu...

    journal_title：BioMed research international

    pub_type： 杂志文章

    doi：10.1155/2014/129048

    authors： Gehmert S,Wenzel C,Loibl M,Brockhoff G,Huber M,Krutsch W,Nerlich M,Gosau M,Klein S,Schreml S,Prantl L,Gehmert S

    更新日期：2014-01-01 00:00:00

  • Adult Onset Vitiligo: Multivariate Analysis Suggests the Need for a Thyroid Screening.

    abstract：:Background. There are limited epidemiological studies evaluating the effect of age at onset on disease features in vitiligo. Objectives. To identify factors associated with adult onset vitiligo in comparison with childhood onset vitiligo. Patients and Methods. We retrospectively collected medical records of 191 patien...

    journal_title：BioMed research international

    pub_type： 杂志文章

    doi：10.1155/2016/8065765

    authors： Lazzeri L,Colucci R,Cammi A,Dragoni F,Moretti S

    更新日期：2016-01-01 00:00:00

  • Protective Effects of Quercetin on Livers from Mice Exposed to Long-Term Cigarette Smoke.

    abstract：:Cigarette smoke is highly toxic, and it can promote increased production of reactive species and inflammatory response and leads to liver diseases. Quercetin is a flavonoid that displays antioxidant and anti-inflammatory activities in liver diseases. This study aimed at evaluating the protective effects of quercetin o...

    journal_title：BioMed research international

    pub_type： 杂志文章

    doi：10.1155/2020/2196207

    authors： Machado-Junior PA,Araújo NPS,Souza ABF,Castro TF,Oliveira M,Costa GP,Matos NA,Vieira PMA,Talvani A,Bezerra FS,Cangussú SD

    更新日期：2020-11-15 00:00:00

  • Determinants of Neonatal Jaundice among Neonates Admitted to Neonatal Intensive Care Unit in Public General Hospitals of Central Zone, Tigray, Northern Ethiopia, 2019: a Case-Control Study.

    abstract：Background:Neonatal jaundice is common a clinical problem worldwide. Globally, every year, about 1.1 million babies develop severe hyperbilirubinemia with or without bilirubin encephalopathy and the vast majority reside in sub-Saharan Africa and South Asia. Strategies and information on determinants of neonatal jaundic...

    journal_title：BioMed research international

    pub_type： 杂志文章

    doi：10.1155/2020/4743974

    authors： Asefa GG,Gebrewahid TG,Nuguse H,Gebremichael MW,Birhane M,Zereabruk K,Zemicheal TM,Hailay A,Abrha WA,Hadera SA,Hailu AG,Beyene BH,Dagnazgi EA,Tekulu FG,Welay F

    更新日期：2020-10-21 00:00:00

  • Tracking Transplanted Stem Cells Using Magnetic Resonance Imaging and the Nanoparticle Labeling Method in Urology.

    abstract：:A reliable in vivo imaging method to localize transplanted cells and monitor their viability would enable a systematic investigation of cell therapy. Most stem cell transplantation studies have used immunohistological staining, which does not provide information about the migration of transplanted cells in vivo in the...

    journal_title：BioMed research international

    pub_type： 杂志文章,评审

    doi：10.1155/2015/231805

    authors： Kim JH,Lee HJ,Song YS

    更新日期：2015-01-01 00:00:00

  • The investigation of quality of life in 87 Chinese patients with disorders of sex development.

    abstract：OBJECTIVE:In the process of care for disorders of sex development (DSD), clinical decisions should focus on the long-term quality of life (QOL). We sought to investigate the QOL of patients with DSD in China. DESIGN:Case-control study was carried out. PATIENTS:90 patients of DSD participated in the study. Finally, 87...

    journal_title：BioMed research international

    pub_type： 临床试验,杂志文章

    doi：10.1155/2015/342420

    authors： Wang C,Tian Q

    更新日期：2015-01-01 00:00:00

  • Methylation Changes of Primary Tumors, Monolayer, and Spheroid Tissue Culture Environments in Malignant Melanoma and Breast Carcinoma.

    abstract：:Epigenetic changes have major role in the normal development and programming of gene expression. Aberrant methylation results in carcinogenesis. The primary objective of our study is to determine whether primary tumor tissue and cultured tumor cells in 2D and 3D tissue culture systems have the same methylation signatu...

    journal_title：BioMed research international

    pub_type： 杂志文章

    doi：10.1155/2019/1407167

    authors： Şükrüoğlu Erdoğan Ö,Kılıç Erciyas S,Bilir A,Buğra Tunçer Ş,Akdeniz Ödemiş D,Kurul S,Karanlık H,Cabıoğlu N,Yazıcı H

    更新日期：2019-01-17 00:00:00

  • Trunk and shoulder kinematic and kinetic and electromyographic adaptations to slope increase during motorized treadmill propulsion among manual wheelchair users with a spinal cord injury.

    abstract：:The main objective was to quantify the effects of five different slopes on trunk and shoulder kinematics as well as shoulder kinetic and muscular demands during manual wheelchair (MWC) propulsion on a motorized treadmill. Eighteen participants with spinal cord injury propelled their MWC at a self-selected constant spe...

    journal_title：BioMed research international

    pub_type： 杂志文章

    doi：10.1155/2015/636319

    authors： Gagnon D,Babineau AC,Champagne A,Desroches G,Aissaoui R

    更新日期：2015-01-01 00:00:00

  • Propranolol Treatment for Infantile Hemangiomas: Short-Term Adverse Effects and Follow-Up to Age Two.

    abstract：Objective:To analyse the short-term adverse effects (AEs) of propranolol in the treatment of infantile hemangiomas (IHs) and their relevant factors, as well as the relationship between child growth and propranolol. Methods:A total of 506 patients with confirmed or suspected IHs were enrolled, and a total of 439 cases ...

    journal_title：BioMed research international

    pub_type： 杂志文章

    doi：10.1155/2019/2728952

    authors： Li X,Yang K,Li H,Huo R

    更新日期：2019-11-25 00:00:00

  • Data-Driven-Based Approach to Identifying Differentially Methylated Regions Using Modified 1D Ising Model.

    abstract：Background:DNA methylation is essential for regulating gene expression, and the changes of DNA methylation status are commonly discovered in disease. Therefore, identification of differentially methylation patterns, especially differentially methylated regions (DMRs), in two different groups is important for understand...

    journal_title：BioMed research international

    pub_type： 杂志文章

    doi：10.1155/2018/1070645

    authors： Zhang Y,Wang S,Wang X

    更新日期：2018-11-18 00:00:00

  • Recurrence-Associated Multi-RNA Signature to Predict Disease-Free Survival for Ovarian Cancer Patients.

    abstract：:Ovarian cancer (OvCa) is an intractable gynecological malignancy due to the high recurrence rate. Several molecular biomarkers have been previously screened for early identifying patients with a high recurrence risk and poor prognosis. However, all the known studies focused on a single type of RNAs, not integrating va...

    journal_title：BioMed research international

    pub_type： 杂志文章

    doi：10.1155/2020/1618527

    authors： Zhang Y,Ye Q,He J,Chen P,Wan J,Li J,Yang Y,Li X

    更新日期：2020-02-14 00:00:00

  • Automated Classification of Lung Cancer Types from Cytological Images Using Deep Convolutional Neural Networks.

    abstract：:Lung cancer is a leading cause of death worldwide. Currently, in differential diagnosis of lung cancer, accurate classification of cancer types (adenocarcinoma, squamous cell carcinoma, and small cell carcinoma) is required. However, improving the accuracy and stability of diagnosis is challenging. In this study, we d...

    journal_title：BioMed research international

    pub_type： 杂志文章

    doi：10.1155/2017/4067832

    authors： Teramoto A,Tsukamoto T,Kiriyama Y,Fujita H

    更新日期：2017-01-01 00:00:00

  • Hepatoprotective and antioxidant effect of Bauhinia hookeri extract against carbon tetrachloride-induced hepatotoxicity in mice and characterization of its bioactive compounds by HPLC-PDA-ESI-MS/MS.

    abstract：:The hepatoprotective and antioxidant activity of Bauhinia hookeri ethanol extract (BHE) against CCl4-induced liver injury was investigated in mice. BHE was administered (500 and 1000 mg/kg/day) along with CCl4 for 6 weeks. The hepatic marker enzymes: alanine aminotransferase (ALT), aspartate aminotransferase (AST), an...

    journal_title：BioMed research international

    pub_type： 杂志文章

    doi：10.1155/2014/245171

    authors： Al-Sayed E,Martiskainen O,Seif el-Din SH,Sabra AN,Hammam OA,El-Lakkany NM,Abdel-Daim MM

    更新日期：2014-01-01 00:00:00

  • Liver Tumor Segmentation from MR Images Using 3D Fast Marching Algorithm and Single Hidden Layer Feedforward Neural Network.

    abstract：:Objective. Our objective is to develop a computerized scheme for liver tumor segmentation in MR images. Materials and Methods. Our proposed scheme consists of four main stages. Firstly, the region of interest (ROI) image which contains the liver tumor region in the T1-weighted MR image series was extracted by using se...

    journal_title：BioMed research international

    pub_type： 杂志文章

    doi：10.1155/2016/3219068

    authors： Le TN,Bao PT,Huynh HT

    更新日期：2016-01-01 00:00:00

  • A Prospective Noninterventional, Observational Study to Describe the Effectiveness and Safety of Trandolapril and Verapamil Single-Pill Combination in the Management of Patients with Hypertension and Type 2 Diabetes Mellitus: A Harvest TR Study.

    abstract：:Maintaining regular blood pressure control usually requires multidrug regimens rather than monotherapy. The objective of this study was to describe the effectiveness and safety of an angiotensin-converting enzyme inhibitor and a nondihydropyridine calcium channel blocker in a single-tablet combination in patients with...

    journal_title：BioMed research international

    pub_type： 杂志文章

    doi：10.1155/2020/2123601

    authors： Atalar E,Eskin F,Tugtekin HB,Karabulut A,Kanyilmaz S,Kirbiyik H,Ozyildiz AG

    更新日期：2020-09-05 00:00:00