A simple protocol for the screening and preliminary identification of antibodies to human IgA using unpurified proteins in the passive haemagglutination test.


:Experiments have shown that suitably diluted, unpurified IgA paraprotein sera can be substituted for chemically purified IgA proteins in the passive haemagglutination test for the detection of anti-IgA antibodies. The substitution of unpurified IgA paraprotein was made possible by the enhanced serological activity obtained by pre-heating the unpurified immunoglobulin preparations at 56 degrees C for 60 min. Using a small screening panel of suitably coated tanned red cells, various population groups were investigated for the presence of anti-IgA antibodies. Results were similar to those obtained by other workers using purified IgA preparations in the passive haemagglutination test.


Vox Sang


Vox sanguinis


Hunt A,Reed M




Has Abstract


1990-01-01 00:00:00












  • A comparison of in vitro tests for predicting the severity of haemolytic disease of the fetus and newborn.

    abstract::Haemolytic disease of the newborn (HDN) is characterized by the presence of IgG antibodies in the maternal circulation which cause haemolysis in the fetus by crossing the placenta and sensitizing red cells for destruction by macrophages in the fetal spleen. Numerous serological, quantitative and cellular assays have b...

    journal_title:Vox sanguinis

    pub_type: 杂志文章,评审


    authors: Hadley AG

    更新日期:1998-01-01 00:00:00

  • Platelet storage media.

    abstract::Present platelet storage media often designated platelet additive solutions (PAS) basically contain acetate, citrate and phosphate and recently also potassium and magnesium. However, there seems to be an increasing interest in developing PASs that can be used also after further reduction of residual plasma content bel...

    journal_title:Vox sanguinis

    pub_type: 杂志文章,评审


    authors: Gulliksson H

    更新日期:2014-10-01 00:00:00

  • The 24-hour posttransfusion survival and lifespan of autologous baboon red cells treated with inositol hexaphosphate-polyethylene glycol or inositol hexaphosphate-adenosine triphosphate-polyethylene glycol to decrease oxygen affinity.

    abstract::Baboon red cells were treated to reduce oxygen affinity by an osmotic-pulse procedure using dimethyl-sulfoxide. Inositol hexaphosphate (IHP) alone or IHP and adenosine triphosphate (ATP) were incorporated into the red cells in the presence of polyethylene glycol (PEG). The procedure produced variable increases in the ...

    journal_title:Vox sanguinis

    pub_type: 杂志文章


    authors: Franco RS,Weiner M,Wagner K,Martelo OJ,Ragno G,Pivacek LE,Valeri CR

    更新日期:1988-01-01 00:00:00

  • A new red blood cell cold autoantibody (anti-Me)

    abstract::A new red blood cell (RBC) cold autoantibody (anti-Me) is presented which was detected in a patient with autoimmune hemolytic anemia of 'cold type' associated with Waldenström's macroglobulinemia. The autoantibody was of the IgM (Kappa) class, had a moderate titer, and reacted with cord as well as with adult RBC. Its ...

    journal_title:Vox sanguinis

    pub_type: 杂志文章


    authors: Salama A,Pralle H,Mueller-Eckhardt C

    更新日期:1985-01-01 00:00:00

  • Estimation of the infectious viral load required for transfusion-transmitted human T-lymphotropic virus type 1 infection (TT-HTLV-1) and of the effectiveness of leukocyte reduction in preventing TT-HTLV-1.

    abstract:BACKGROUND AND OBJECTIVES:The risk of transfusion-transmitted human T-lymphotropic virus type 1 infection (TT-HTLV-1) after prestorage leucocyte reduction (LR) remains unknown, as the proviral load in the blood component that would cause TT-HTLV-1 is undetermined. On the basis of the distribution of HTLV-1 proviral loa...

    journal_title:Vox sanguinis

    pub_type: 杂志文章


    authors: Sobata R,Matsumoto C,Uchida S,Suzuki Y,Satake M,Tadokoro K

    更新日期:2015-08-01 00:00:00

  • Non-HLA lymphocytotoxic antibodies in HIV-seropositive and HIV-seronegative hemophiliacs.

    abstract::We tested serum samples from 11 HIV-seropositive and 32 HIV-seronegative Italian patients with hemophilia A or B for the presence of lymphocytotoxic antibodies (LCTAs). No patients had the acquired immune deficiency syndrome and all had received over many years heat-untreated commercial clotting factors. LCTAs directe...

    journal_title:Vox sanguinis

    pub_type: 杂志文章


    authors: Conte R,Giovanardi L,Tazzari PL,De Rosa V,Rodorigo G,Re MC

    更新日期:1988-01-01 00:00:00

  • Detection of anti-Lea in Le(a-b+) individuals by kinetic ELISA.

    abstract::Utilizing a kinetic enzyme-linked antiglobulin binding assay, eight examples of anti-Lea were detected in individuals whose Lewis phenotype is Le(a-b+). These antibodies were not detectable by routine hemagglutination techniques, but were indistinguishable from anti-Lea made by Le(a-b-) individuals in terms of their i...

    journal_title:Vox sanguinis

    pub_type: 杂志文章


    authors: Cowles JW,Spitalnik SL,Blumberg N

    更新日期:1986-01-01 00:00:00

  • Detection of early anti-p24 HIV responses in EIA- and immunoblot-negative individuals. Implications for confirmatory testing.

    abstract::A sensitive and specific radioimmunoprecipitation assay was developed for the detection and analysis of anti-HIV antibody response in human sera with the use of 125I-labelled purified HIV proteins with subsequent sodium-dodecylsulfate gel electrophoresis (125I-RIPA). The 125I-RIPA was shown to be as specific but at le...

    journal_title:Vox sanguinis

    pub_type: 杂志文章


    authors: Huisman JG,Winkel IN,Lelie PN,Tersmette M,Goudsmit J,Miedema F

    更新日期:1987-01-01 00:00:00

  • Red-blood-cell manufacturing methods and storage solutions differentially induce pulmonary cell activation.

    abstract:BACKGROUND AND OBJECTIVES:Red-blood-cell (RBC) transfusion is associated with lung injury, which is further exacerbated by mechanical ventilation. Manufacturing methods of blood products differ globally and may play a role in the induction of pulmonary cell activation through alteration of the immunomodulatory property...

    journal_title:Vox sanguinis

    pub_type: 杂志文章


    authors: Wirtz MR,Almizraq RJ,Weber NC,Norris PJ,Pandey S,Spinella PC,Muszynski JA,P Acker J,Juffermans NP

    更新日期:2020-07-01 00:00:00

  • Increase in glycocalicin levels in platelet concentrates stored in plasma or synthetic medium for 8 days: comparison with other platelet activation markers.

    abstract:BACKGROUND AND OBJECTIVES:Glycocalicin (GC) is a proteolytic fragment of GpIb and can conveniently be measured in supernatants of platelet concentrates (PCs) by means of a sandwich ELISA. Because of the convenience of the assay and easy sample storage, we tested its suitability as a sensitive platelet activation parame...

    journal_title:Vox sanguinis

    pub_type: 杂志文章


    authors: Kostelijk EH,Folman CC,Gouwerok CW,Kramer CM,Verhoeven AJ,de Korte D

    更新日期:2000-01-01 00:00:00

  • Impact of tobacco smoking on platelet function in apheresis products in vitro.

    abstract:BACKGROUND AND OBJECTIVES:The aim of this study was to analyse the platelet function, over a 5-day time-period, of apheresis-derived platelet concentrates obtained from smokers and non-smokers. MATERIALS AND METHODS:Smoker and non-smoker plateletpheresis products were investigated on days 1, 3 and 5 of storage. Recept...

    journal_title:Vox sanguinis

    pub_type: 杂志文章


    authors: Scheinichen D,Heuft HG,Renken C,Jüttner B,Jaeger K,Schürholz T,Blasczyk R,Piepenbrock S,Elsner HA

    更新日期:2004-05-01 00:00:00

  • Factors influencing plasma transfusion practices in paediatric intensive care units around the world.

    abstract:BACKGROUND AND OBJECTIVES:Plasma transfusions are a frequent treatment worldwide, but many studies have reported a wide variation in the indications to transfuse. Recently, an international paediatric study also showed wide variation in frequency in the use of plasma transfusions: 25% of the centres transfused plasma t...

    journal_title:Vox sanguinis

    pub_type: 杂志文章


    authors: Karam O,Demaret P,Duhamel A,Shefler A,Spinella PC,Tucci M,Leteurtre S,Stanworth SJ,PlasmaTV investigators.

    更新日期:2017-02-01 00:00:00

  • Posttransfusion non-A, non-B hepatitis after cardiac surgery: a prospective study.

    abstract::In a prospective study of 50 recipients of HBsAg-negative blood who had undergone cardiac surgery, 4 (8%) developed acute non-A, non-B hepatitis. The patients who developed hepatitis had received significantly more units of blood or blood products than the patients who had no hepatitis. The incubation period of the di...

    journal_title:Vox sanguinis

    pub_type: 杂志文章


    authors: Tur-Kaspa R,Shimon DV,Shalit M,Adler R,Shraga S,Manny N,Morag A,Eliakim M

    更新日期:1983-01-01 00:00:00

  • Red cells from the original JAL+ proband are also DAK+ and STEM+.

    abstract:BACKGROUND:The low-prevalence Rh antigen, JAL, was named after the index case, Mr. J. Allen. Based on reactivity of seven multi-specific sera with his RBCs, it was apparent that they express at least one additional low-prevalence antigen. The purpose of this study was to investigate the other low-prevalence antigen(s) ...

    journal_title:Vox sanguinis

    pub_type: 杂志文章


    authors: Hue-Roye K,Reid ME,Westhoff CM,Lomas-Francis C

    更新日期:2011-07-01 00:00:00

  • The effect of immunoadsorption with the Immusorba TR-350 column on coagulation compared to plasma exchange.

    abstract:BACKGROUND AND OBJECTIVES:Plasma exchange (PE) and immunoadsorption with the Immusorba TR-350 column (IA) are used to remove autoantibodies from plasma in acute neurological autoimmune disorders. The impact of IA on coagulation and on low molecular weight heparin (LMWH) levels in comparison with PE was investigated. P...

    journal_title:Vox sanguinis

    pub_type: 杂志文章


    authors: Koessler J,Kobsar A,Kuhn S,Koessler A,Yilmaz P,Weinig E,Putz E,Boeck M,Klinker E

    更新日期:2015-01-01 00:00:00

  • Estimation of the number of binding sites for a murine monoclonal anti-Lub on human erythrocytes.

    abstract::The number of Lub antigen sites on the human erythrocyte membrane, as recognised by the murine monoclonal antibody BRIC-108, has been determined. The number of antibody molecules bound per cell on one example of cells of the phenotype Lu(a-b-) with recessive inheritance was an average of around 200 in replicate determ...

    journal_title:Vox sanguinis

    pub_type: 杂志文章


    authors: Merry AH,Gardner B,Parsons SF,Anstee DJ

    更新日期:1987-01-01 00:00:00

  • International standards for minimum potency of anti-A and anti-B blood grouping reagents: evaluation of candidate preparations in an international collaborative study.

    abstract:BACKGROUND AND OBJECTIVES:The aim of the study was to evaluate lyophilized monoclonal IgM anti-A and anti-B preparations for use as international standards (IS) to specify recommended minimum potencies of anti-A and anti-B blood grouping reagents in tube tests. MATERIALS AND METHODS:The candidate IS for minimum potenc...

    journal_title:Vox sanguinis

    pub_type: 杂志文章


    authors: Thorpe SJ,Fox B,Heath AB,Scott M,de Haas M,Kochman S,Padilla A

    更新日期:2006-11-01 00:00:00

  • Loss of phosphotyrosine phosphatase activity and changes in the tyrosine phosphorylation state of proteins after storage of sheep platelets in plasma or Seto solution at 4 degrees C.

    abstract:BACKGROUND AND OBJECTIVES:During platelet storage an array of deleterious changes occur, through mechanisms not fully understood, which impair platelet haemostasis. Transfused platelets should maintain the integrated networks of signalling pathways that regulate platelet activation and functionality. We hypothesized th...

    journal_title:Vox sanguinis

    pub_type: 杂志文章


    authors: Hernández-Hernández A,Sánchez-Bernal C,Rodríguez MC,Gómez FP,Llanillo M,Sánchez-Yagüe J

    更新日期:2001-11-01 00:00:00

  • Lactated Ringer's solution versus hydroxyethyl starch for volume replacement in autologous blood donors with cardiovascular disease: a controlled, randomized trial.

    abstract:BACKGROUND AND OBJECTIVES:The study was designed to evaluate whether volume replacement following blood donation can prevent arterial hypotension in autologous blood donors with cardiovascular disease. MATERIALS AND METHODS:One hundred nineteen autologous blood donors with known cardiovascular disease were randomly al...

    journal_title:Vox sanguinis

    pub_type: 临床试验,杂志文章,随机对照试验


    authors: Kasper SM,Dahlmann H,Mellinghoff H,Ellering J,Baumann M,Buzello W

    更新日期:1998-01-01 00:00:00

  • Weak D alleles in Japanese: a c.960G>A silent mutation in exon 7 of the RHD gene that affects D expression.

    abstract:BACKGROUND AND OBJECTIVES:The molecular basis of the weak D phenotype has been investigated for many years, and more than 80 different alleles producing weak D phenotypes have been identified. Most alleles producing weak D phenotypes have a single missense mutation in exons corresponding to a transmembrane domain of th...

    journal_title:Vox sanguinis

    pub_type: 杂志文章


    authors: Ogasawara K,Sasaki K,Isa K,Tsuneyama H,Uchikawa M,Satake M,Tadokoro K

    更新日期:2016-02-01 00:00:00

  • Persistent aggregates in apheresis platelet concentrates.

    abstract:BACKGROUND:Aggregates often appear during apheresis. Sometimes, these persist throughout storage, causing product wastage. This study assessed product quality of apheresis concentrates containing persistent aggregates (PA) and aimed to identify the factors that contribute to their formation. METHODS:Donation (n = 180)...

    journal_title:Vox sanguinis

    pub_type: 杂志文章


    authors: Feys HB,Coene J,Devloo R,Van Aelst B,Pottel H,Vandekerckhove P,Compernolle V

    更新日期:2015-05-01 00:00:00

  • White-blood-cell-containing allogeneic blood transfusion, postoperative infection and mortality: a meta-analysis of observational 'before-and-after' studies.

    abstract:BACKGROUND AND OBJECTIVES:An association of white blood cell (WBC) reduction with decreased mortality was reported by one observational, before-and-after study. A meta-analysis was undertaken to examine whether this finding is supported by all the evidence currently available from before-and-after studies, and whether ...

    journal_title:Vox sanguinis

    pub_type: 杂志文章,meta分析


    authors: Vamvakas EC

    更新日期:2004-02-01 00:00:00

  • Transfusion results of filtered and subsequently stored random platelet suspensions prepared from buffy coats.

    abstract::There is almost general agreement that removal of leukocytes from blood components reduces the incidence of HLA-antibody formation and refractoriness to random platelet transfusions. Recently filters have become available, which are able to reduce leukocyte contamination in platelet suspensions with acceptable platele...

    journal_title:Vox sanguinis

    pub_type: 杂志文章


    authors: Novotny VM,van Doorn R,Rozier Y,D'Amaro J,Harvey MS,Brand A

    更新日期:1992-01-01 00:00:00

  • Study of coagulation function in thawed apheresis plasma for photochemical treatment by amotosalen and UVA.

    abstract:BACKGROUND AND OBJECTIVES:Photochemical treatment (PCT) based on amotosalen and ultraviolet A light (UVA) demonstrated a wide range of pathogen inactivation. However, coagulation proteins are affected by this treatment. The aim of this study was to evaluate the coagulation parameters in apheresis plasma units after tha...

    journal_title:Vox sanguinis

    pub_type: 杂志文章


    authors: de Valensart N,Rapaille A,Goossenaerts E,Sondag-Thull D,Deneys V

    更新日期:2009-04-01 00:00:00

  • False reactions in radioimmunoassay for viral hepatitis B markers in patients suffering from coagulation disorders.

    abstract::Simultaneous occurrence of hepatitis B surface antigen (HBsAg) and antibodies to HBsAg (anti-HBs) has been frequently described in hemophiliac patients. During our working procedures, this phenomenon was also observed. Interference of coagulation disorders with radioimmunoassay (RIA) used in the detection of viral hep...

    journal_title:Vox sanguinis

    pub_type: 杂志文章


    authors: Grangeot-Keros L,Lambert T,Dubreuil P,Briantais MJ,Pillot J

    更新日期:1982-03-01 00:00:00

  • The strategies to reduce iron deficiency in blood donors randomized trial: design, enrolment and early retention.

    abstract:BACKGROUND AND OBJECTIVES:Repeated blood donation produces iron deficiency. Changes in dietary iron intake do not prevent donation-induced iron deficiency. Prolonging the interdonation interval or using oral iron supplements can mitigate donation-induced iron deficiency. The most effective operational methods for reduc...

    journal_title:Vox sanguinis

    pub_type: 临床试验,杂志文章,随机对照试验


    authors: Bialkowski W,Bryant BJ,Schlumpf KS,Wright DJ,Birch R,Kiss JE,D'Andrea P,Cable RG,Spencer BR,Vij V,Mast AE

    更新日期:2015-02-01 00:00:00

  • A newly devised flow cytometric antibody binding assay helps evaluation of dithiothreitol treatment for the inactivation of CD38 on red blood cells.

    abstract:BACKGROUND AND OBJECTIVES:Anti-CD38 monoclonal antibodies, including daratumumab and isatuximab, often interfere with pretransfusion testing. Dithiothreitol (DTT) treatment of red blood cells (RBCs) negates this interference. However, the optimum DTT concentration and treatment time have not been well defined. Here, we...

    journal_title:Vox sanguinis

    pub_type: 杂志文章


    authors: Furumaki H,Takeshita A,Ohto H,Yamada C,Fujihara H,Ishizuka K,Shibata H,Shinba T,Nemoto N,Ino K,Ozawa A,Watanabe H,Kawabata K,Obata Y

    更新日期:2020-12-13 00:00:00

  • Immunofluorescent staining of platelet suspensions and detection of antiplatelet antibody in patients with idiopathic thrombocytopenic purpura.

    abstract::Direct immunofluorescent staining of 31 specimens of platelets obtained from 13 cases of idiopathic thrombocytopenic purpura (ITP) revealed positive staining on the surface of platelets for both immunoglobulins (Igs) and human B1C globulin in 9 specimens, for only Igs in 1 specimen and for human B1C alone in 5 specime...

    journal_title:Vox sanguinis

    pub_type: 杂志文章


    authors: Boonpucknavig S,Vuttivirojana O,Srichaikul T

    更新日期:1979-01-01 00:00:00

  • A novel mutation of the GATA site in the erythroid cell-specific regulatory element of the ABO gene in a blood donor with the Am B phenotype.

    abstract::The Am and Bm phenotypes are characterized by weak expression of the A or B antigens, respectively, by red blood cells with a normal expression by the saliva of secretors. Deletion of the regulatory element in the first intron of the ABO gene and disruption of the GATA motif in the element were found to be responsible...

    journal_title:Vox sanguinis

    pub_type: 杂志文章


    authors: Oda A,Isa K,Ogasawara K,Kameyama K,Okuda K,Hirashima M,Ishii H,Kimura K,Matsukura H,Hirayama F,Kawa K

    更新日期:2015-05-01 00:00:00

  • In vitro phagocytosis of liquid-stored red blood cells requires serum and can be inhibited with fucoidan and dextran sulphate.

    abstract:BACKGROUND AND OBJECTIVES:Red-blood-cells (RBCs) undergo structural and metabolic changes with prolonged storage, which ultimately may decrease their survival after transfusion. Although the storage-induced damage to RBCs has been rather well described biochemically, little is known about the mechanisms underlying the ...

    journal_title:Vox sanguinis

    pub_type: 杂志文章


    authors: Hult A,Toss F,Malm C,Oldenborg PA

    更新日期:2020-11-01 00:00:00