A simple protocol for the screening and preliminary identification of antibodies to human IgA using unpurified proteins in the passive haemagglutination test.

Abstract:

:Experiments have shown that suitably diluted, unpurified IgA paraprotein sera can be substituted for chemically purified IgA proteins in the passive haemagglutination test for the detection of anti-IgA antibodies. The substitution of unpurified IgA paraprotein was made possible by the enhanced serological activity obtained by pre-heating the unpurified immunoglobulin preparations at 56 degrees C for 60 min. Using a small screening panel of suitably coated tanned red cells, various population groups were investigated for the presence of anti-IgA antibodies. Results were similar to those obtained by other workers using purified IgA preparations in the passive haemagglutination test.

journal_name

Vox Sang

journal_title

Vox sanguinis

authors

Hunt A,Reed M

doi

10.1111/j.1423-0410.1990.tb02110.x

subject

Has Abstract

pub_date

1990-01-01 00:00:00

pages

30-3

issue

1

eissn

0042-9007

issn

1423-0410

journal_volume

59

pub_type

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