A multi-centre study on the performance of the molecular genotyping platform ID RHD XT for resolving serological weak RhD phenotype in routine clinical practice.

abstract：BACKGROUND:Immune haemolysis in liver transplant (LT) can occur due to autoantibodies and alloantibodies. The aim of this study was to evaluate the prevalence and risk factors for immune haemolysis in LT. METHODS:A total of 175 consecutive patients were included. Multiorgan recipients were excluded. Samples, from befo...

journal_title：Vox sanguinis

authors： Brunetta DM,de Barros Carlos LM,Da Silva VFP,de Oliveira Alves TM,Macedo ÊS,Coelho GR,Vasconcelos JBM,De Francesco Daher E,Garcia JHP

更新日期：2020-01-01 00:00:00

abstract：:The Am and Bm phenotypes are characterized by weak expression of the A or B antigens, respectively, by red blood cells with a normal expression by the saliva of secretors. Deletion of the regulatory element in the first intron of the ABO gene and disruption of the GATA motif in the element were found to be responsible...

journal_title：Vox sanguinis

authors： Oda A,Isa K,Ogasawara K,Kameyama K,Okuda K,Hirashima M,Ishii H,Kimura K,Matsukura H,Hirayama F,Kawa K

更新日期：2015-05-01 00:00:00

abstract：:A population of human erythrocytes (free A3 cells) which was entirely unagglutinable with anti-A hemagglutinins was successfully separated from human blood group A3 erythrocytes by affinity chromatography on a column of lima bean anti-A hemagglutinin (LBH)-Sepharose. The hemagglutinating and the binding properties of ...

journal_title：Vox sanguinis

authors： Oguchi Y,Kawaguchi T,Suzuta T,Osawa T

更新日期：1978-01-01 00:00:00

abstract：OBJECTIVES:Baxter's methylene blue (MB) photoinactivation of fresh frozen plasma (FFP) is a method that effectively removes any intracellular potentially infectious agent, such as prions, by filtration combined with an improved photochemical virus inactivation on a single unit of FFP. The purpose of this study was to d...

journal_title：Vox sanguinis

authors： Simonsen AC,Sørensen H

更新日期：1999-01-01 00:00:00

abstract：:When the blood group H sites of cord erythrocytes obtained from newborn infants of groups O, A, B and AB were labelled specifically by incubation of the whole cells with the A1 gene dependent alpha-N-acetylgalactosaminyl transferase in the presence of UDP-N-acetyl [14C]-galactosamine, the incorporation of radioactivit...

journal_title：Vox sanguinis

authors： Schenkel-Brunner H

更新日期：1980-06-01 00:00:00

abstract：BACKGROUND AND OBJECTIVES:The aim of the study was to replace the 1(st) World Health Organization International Standard for parvovirus B19 DNA for nucleic acid amplification technique (NAT)-based assays (code 99/800). Two lyophilized preparations (coded 99/800 and 99/802) had been evaluated in the original collaborati...

journal_title：Vox sanguinis

authors： Baylis SA,Chudy M,Blümel J,Pisani G,Candotti D,José M,Heath AB

更新日期：2010-04-01 00:00:00

abstract：OBJECTIVES:We have developed and optimised a new flow-cytometric method for the measurement of the Fc function of intravenous immunoglobulin (IVIg) preparations, which is important in predicting the effector function of immunoglobulin (Ig) in such preparations. MATERIALS AND METHODS:Ig was bound to a monocytic cell li...

journal_title：Vox sanguinis

authors： Ramasamy I,Tran E,Charnock A,Farrugia A

更新日期：2000-01-01 00:00:00

abstract：BACKGROUND AND OBJECTIVES:The low prevalence antigen, Be(a), is produced by a complex that also produces weak c, e and f (ce). We report here the molecular basis associated with Be(a) antigen expression. MATERIALS AND METHODS:Peripheral blood samples from four Be(a+) probands were tested. Haemagglutination, gDNA extra...

journal_title：Vox sanguinis

authors： Hue-Roye K,O'Shea K,Gillett R,Wadsworth LD,Hume H,Barnes J,Kinney J,Hodgins K,Fuchisawa A,Lomas-Francis C,Reid M

更新日期：2010-04-01 00:00:00

abstract：:Detailed serological investigation of five examples of anti-Jka revealed considerable heterogeneity in their characteristics. All the sera were investigated by the antiglobulin technique, with and without complement and by a range of sensitization and testing methods. Three were readily detected in the presence of com...

journal_title：Vox sanguinis

authors： Howell P,Giles CM

更新日期：1983-01-01 00:00:00

abstract：:Extracts of seeds from 79 plant species were tested for agglutinating and lytic activity with the red cells of ten vertebrate species (man, monkey, rabbit, rat, goat, sheep, guinea pig, horse, fowl, dog). Of these extracts, 24 were hemagglutinating and 15 were hemolytic. The results suggest that some lectins can serve...

journal_title：Vox sanguinis

authors： Bhalla V,Gaur V,Bhatia K

更新日期：1978-01-01 00:00:00

abstract：:In a prospective study of 50 recipients of HBsAg-negative blood who had undergone cardiac surgery, 4 (8%) developed acute non-A, non-B hepatitis. The patients who developed hepatitis had received significantly more units of blood or blood products than the patients who had no hepatitis. The incubation period of the di...

journal_title：Vox sanguinis

authors： Tur-Kaspa R,Shimon DV,Shalit M,Adler R,Shraga S,Manny N,Morag A,Eliakim M

更新日期：1983-01-01 00:00:00

abstract：:A major drawback in the use of platelet transfusions is the development of platelet refractoriness that is usually caused by alloimmunization provoked by leukocytes present in the blood products. We evaluated the development of alloimmunization both by serological demonstration of HLA antibodies and by clinical demons...

journal_title：Vox sanguinis

authors： Saarinen UM,Koskimies S,Myllylä G

更新日期：1993-01-01 00:00:00

abstract：:Platelet antibodies were looked for in 47 patients with autoimmune thrombocytopenia using a modification of the enzyme-linked assay previously described. Surface-bound antibodies measured as increased platelet-associated IgG were found in 32 (68%) of the patients. After incubation in test sera, the platelet-associated...

journal_title：Vox sanguinis

authors： Hegde UM,Bowes A,Powell DK,Joyner MV

更新日期：1981-11-01 00:00:00

abstract：:Four commercial intravenous immunoglobulin G (IGIV) preparations, Sandoglobulin (Sandoz), Intraglobin (Biotest), Gammonativ (Kabivitrum) and Gammaogard (Kabivitrum) showed percent inhibition of Fc receptor-mediated phagocytosis of anti-Rh(D)-coated erythrocytes as follows: 45 +/- 14 SD, 41 +/- 23 SD, 35 +/- 14 SD, 25 ...

journal_title：Vox sanguinis

authors： Sheth KV,al-Sedairy S,Lee J

更新日期：1993-01-01 00:00:00

abstract：:Interest in immunogenetics originated from two streams: (a) histocompatibility in mouse and man, and (b) inheritance of specific immune responses in the guinea pig and mouse. In the mouse, there are genes associated with the major histocompatibility complex (MHC) which (i) code for antigens determining allograft respo...

journal_title：Vox sanguinis

authors： Mackay IR,Wittingham S,Tait B

更新日期：1977-01-01 00:00:00

abstract：:Recently, we have shown that thiopyrylium has robust inactivation capabilities against a broad spectrum of pathogens in the human red blood cell (RBC) suspensions while retaining key RBC in vitro properties. The 24-h recovery and survival of canine red cells were measured upon autologus reinfusion of control and photo...

journal_title：Vox sanguinis

authors： Skripchenko A,Balch A,Mackin A,Wagner SJ

更新日期：2007-02-01 00:00:00

abstract：:Blood transfusion practices have evolved empirically, with few or no research data supporting them. In the past several decades, fuelled by fears of infection and reports of mistakes, patients have increasingly demanded their rights to choice, both in the components given to them as well as to refuse to receive these ...

journal_title：Vox sanguinis

authors： Sazama K

更新日期：2007-02-01 00:00:00

abstract：:To determine what effect different preparative protocols and different platelet storage bags have on in vitro release, platelet concentrates were prepared according to two different protocols (group I: 2,200 g for 3.5 min followed by 4,900 g for 5 min; group II: 1,000 g for 9 min and 3,000 g for 20 min). The storage b...

journal_title：Vox sanguinis

authors： Snyder EL,Koerner TA Jr,Hezzey A

更新日期：1982-01-01 00:00:00

abstract：:In a 2-year study involving 133 premature low birth weight (less than 1,500 g) infants, the impact of CMV infection via blood transfusion was assessed. 8.4% (7 out of 83) of transfused infants and 10% (7 out of 70) of those exposed to seropositive blood acquired CMV. In those less than 1,250 g the infection rate rose ...

journal_title：Vox sanguinis

authors： Galea G,Urbaniak SJ

更新日期：1992-01-01 00:00:00

abstract：:Experiments have shown that suitably diluted, unpurified IgA paraprotein sera can be substituted for chemically purified IgA proteins in the passive haemagglutination test for the detection of anti-IgA antibodies. The substitution of unpurified IgA paraprotein was made possible by the enhanced serological activity obt...

journal_title：Vox sanguinis

authors： Hunt A,Reed M

更新日期：1990-01-01 00:00:00

abstract：:A method of pasteurising a concentrate of factor I (C3b inactivator) prepared from human blood plasma is described. As well as obtaining a high yield of factor I by this virus inactivation procedure it is furthermore possible, by adjustment of the stabiliser concentration, to obtain a very pronounced reduction in the ...

journal_title：Vox sanguinis

authors： Alsøe KO,Kihl J

更新日期：1989-01-01 00:00:00

abstract：OBJECTIVES:Positive direct antiglobulin tests (DAT) occur occasionally, without obvious cause, among normal blood donors. This is sometimes associated with a false-positive serologic test for syphilis. Positive DATs due to antiphospholipid antibodies (APAbs) are well documented in the antiphospholipid syndrome with sys...

journal_title：Vox sanguinis

authors： Win N,Islam SI,Peterkin MA,Walker ID

更新日期：1997-01-01 00:00:00

abstract：:Platelet function in thrombosis and haemostasis is reasonably well understood at the molecular level with respect to the proteins involved in cellular structure, signalling networks and platelet interaction with clotting factors and other cells. However, the natural history of these proteins has only recently garnered...

journal_title：Vox sanguinis

authors： Schubert P,Devine DV

更新日期：2010-08-01 00:00:00

abstract：:Isoagglutinins were investigated following ABO-incompatible bone marrow transplantation. In major incompatibility anti-A tends to require more time to disappear than anti-B. Correspondingly, A erythrocytes require more time to become demonstrable. There is no such difference in major plus minor incompatibility. In min...

journal_title：Vox sanguinis

authors： Wernet D,Mayer G

更新日期：1992-01-01 00:00:00

abstract：BACKGROUND:The Mirasol Pathogen Reduction Technology System (PRT) for Plasma (CaridianBCT) is based on a riboflavin and UV light treatment process resulting in pathogen inactivation due to irreversible, photochemically induced damage of nucleic acids. This study evaluated the in vitro protein quality of plasma products...

journal_title：Vox sanguinis

authors： Bihm DJ,Ettinger A,Buytaert-Hoefen KA,Hendrix BK,Maldonado-Codina G,Rock G,Giclas PC,Goodrich RP

更新日期：2010-02-01 00:00:00

abstract：BACKGROUND AND OBJECTIVES:To prove the feasibility of a semi-automated cross-match procedure using a commercially available solid-phase microplate test and standard laboratory equipment. MATERIAL AND METHODS:The new procedure was evaluated against the conventional spin tube technique and the gel centrifugation system....

journal_title：Vox sanguinis

authors： Ostendorf N,Niefhoff D,Cassens U,Sibrowski W

更新日期：2001-05-01 00:00:00

abstract：:The results obtained in a multicentre clinical trial of an enzyme immunoassay (EIA) method for hepatitis B surface antigen (HBsAg) (65,451 sera tested) have demonstrated that this new test has a significantly higher sensitivity than a reversed haemagglutination test (rHA). In a part of the trial, EIA was also compared...

journal_title：Vox sanguinis

authors： Kacaki J,Wolters G,Kuijpers L,Stulemeyer S

更新日期：1978-07-01 00:00:00

abstract：BACKGROUND AND OBJECTIVES:A multicentre trial was set up to evaluate the performance of a new leucodepletion protocol. MATERIALS AND METHODS:Filtration at high haematocrit was started during collection of red blood cell (RBC) products by apheresis with Trima. SAG-M was added after filtration through the filter. Haemat...

journal_title：Vox sanguinis

authors： Soli M,Blanco L,Riggert J,Martínez-Clavel A,Lucas C,Lunghi M,Belloni M,Wolf C,van Waeg G,Antoon M

更新日期：2001-01-01 00:00:00

abstract：:A chromatographical study was made of eight antisera containing either anti-HL-A antibodies, cold lymphocytotoxins, or a mixture of both types of antibodies. The results vary according to the sera obtained. Usually cold lymphocytotoxins are sited in the IgM peak and anti-HL-A antibodies in the IgG peak, but there are ...

journal_title：Vox sanguinis

authors： Mayer S,Hauptmann G,Tongio MM

更新日期：1976-01-01 00:00:00

abstract：:A retrospective analysis was conducted on 20 D(-) liver transplant (LT) recipients transfused with D(+) RBCs perioperatively and screened for RBC antibodies between 2 and 6 months later. None developed anti-D detectable by the indirect antiglobulin test. Two patients produced weak anti-D that reacted only with papain-...

journal_title：Vox sanguinis

authors： Burin des Roziers N,Ibanez C,Samuel D,Francoz C,Idri S,François A,Mortelecque R,Bierling P,Pirenne F

更新日期：2016-07-01 00:00:00