The low prevalence Rh antigen Be(a) (Rh36) is associated with RHCE*ce 662C>G in exon 5, which is predicted to encode Rhce 221Arg.

Abstract:

BACKGROUND AND OBJECTIVES:The low prevalence antigen, Be(a), is produced by a complex that also produces weak c, e and f (ce). We report here the molecular basis associated with Be(a) antigen expression. MATERIALS AND METHODS:Peripheral blood samples from four Be(a+) probands were tested. Haemagglutination, gDNA extraction, PCR-based assays, reticulocyte RNA isolation, Rh-cDNA analyses, and sequencing were performed by standard procedures. RESULTS:RBCs from Probands 1 and 3 were D-C-E-c+e+, and from Probands 2 and 4 were D+C+E-c+(W)e+. In proband 1, cDNA sequencing of RHCE revealed heterozygosity of nucleotide (nt) 662C/G in exon 5 of RHCE*ce. No other nucleotide changes were observed. As the 662C>G nucleotide change ablates a MscI restriction enzyme cleavage site, PCR-RFLP analysis was performed and the RHCE*ce nt 662C/G heterozygosity was detected on gDNA from the four probands and two children from both Proband 3 and Proband 4. CONCLUSION:The low prevalence Rh antigen, Be(a), is associated with a single nucleotide change in exon 5 of RHCE*ce; that of 662C>G and this change is predicted to alter proline at amino acid position 221 of Rhce to arginine. The fundamental differences in the properties of these two amino acids may impose a steric and/or charge-related effect on the protein, and thereby provide an explanation for the weakened expression of c, e and f (ce) antigens in the Be(a) phenotype.

journal_name

Vox Sang

journal_title

Vox sanguinis

authors

Hue-Roye K,O'Shea K,Gillett R,Wadsworth LD,Hume H,Barnes J,Kinney J,Hodgins K,Fuchisawa A,Lomas-Francis C,Reid M

doi

10.1111/j.1423-0410.2009.01277.x

subject

Has Abstract

pub_date

2010-04-01 00:00:00

pages

e263-8

issue

3 Pt 1

eissn

0042-9007

issn

1423-0410

pii

VOX1277

journal_volume

98

pub_type

杂志文章
  • Iron deficiency among French whole-blood donors: first assessment and identification of predictive factors.

    abstract:BACKGROUND:The objectives of this study are to estimate the prevalence of iron deficiency (ID) among French whole-blood (WB) donors to identify factors associated with ID and to generate decision trees. STUDY DESIGN AND METHODS:A prospective National multicentre study was performed on WB donors from March 11, to April...

    journal_title:Vox sanguinis

    pub_type: 杂志文章

    doi:10.1111/vox.12991

    authors: Fillet AM,Martinaud C,Malard L,Le Cam S,Hejl C,Chenus F,Woimant G,Chueca M,Jacquot E,Besiers C,Morel P,Djoudi R,Garrabé E,Gross S

    更新日期:2021-01-01 00:00:00

  • Standardization of bromelin for use in routine one-stage antibody screening.

    abstract::A simple standardization method for bromelin used in routine one-stage antibody screening is described. Bromelin proteinase activity was assayed using casein as the substrate, and converted to units. The use of proteinase activity units for standardization of bromelin resolves differences between commercial preparatio...

    journal_title:Vox sanguinis

    pub_type: 杂志文章

    doi:10.1111/j.1423-0410.1987.tb02991.x

    authors: Mazda T,Ogasawara K,Nakata K,Shimizu M

    更新日期:1987-01-01 00:00:00

  • Metagenomics analysis of the virome of 300 concentrates from a Swiss platelet bank.

    abstract:BACKGROUND AND OBJECTIVES:Platelet concentrates are frequently transfused to patients with reduced immunity. An exhaustive description of their viral content is needed to prevent unwanted infection. MATERIAL AND METHODS:To track viral sequences, a shotgun metagenomics approach was used on a bank of 300 platelets conce...

    journal_title:Vox sanguinis

    pub_type: 杂志文章

    doi:10.1111/vox.12695

    authors: Brito F,Cordey S,Delwart E,Deng X,Tirefort D,Lemoine-Chaduc C,Zdobnov E,Lecompte T,Kaiser L,Waldvogel-Abramowski S,Preynat-Seauve O

    更新日期:2018-07-18 00:00:00

  • Red-blood-cell alloimmunization and prophylactic antigen matching for transfusion in patients with warm autoantibodies.

    abstract:BACKGROUND:Warm autoantibodies (WAA) are antibodies that react with an antigen on a patient's own red-blood-cells and can complicate compatibility testing whether or not they cause clinical haemolysis. The goal of this study was to understand the overall prevalence of WAA, the risk of RBC alloimmunization and determine...

    journal_title:Vox sanguinis

    pub_type: 杂志文章

    doi:10.1111/vox.12914

    authors: Delaney M,Apelseth TO,Bonet Bub C,Cohn CS,Dunbar NM,Mauro Kutner J,Murphy M,Perelman I,Selleng K,Staves J,Wendel S,Ziman A,Biomedical Excellence for Safer Transfusion (BEST) Collaborative.

    更新日期:2020-08-01 00:00:00

  • Plasmapheresis technology.

    abstract::Manual plasmapheresis is done worldwide on both paid donors, and to a much smaller extent on volunteer donors in order to generate source plasma to be fractionated into albumin, clotting factor concentrates, and gamma globulin. Automated technology has now been developed utilizing both centrifugal, and membrane separa...

    journal_title:Vox sanguinis

    pub_type: 杂志文章

    doi:10.1111/j.1423-0410.1986.tb01992.x

    authors: Gilcher RO

    更新日期:1986-01-01 00:00:00

  • Mimicking anti-S simulating a delayed transfusion reaction.

    abstract::An autoantibody mimicking anti-S specificity is described in an S-s+ patient. The antibody was detected 2 weeks after transfusion. The direct antiglobulin test (DAT) was positive and the antibody was thought to be an alloantibody formed after the transfusion. Eighteen months later, the DAT was still positive and anti-...

    journal_title:Vox sanguinis

    pub_type: 杂志文章

    doi:10.1111/j.1423-0410.1987.tb04944.x

    authors: Puig N,Carbonell F,Soler MA,Perez MT,Arriaga F,Marty ML

    更新日期:1987-01-01 00:00:00

  • Association of ABO gene mutations resulting in a rare B subgroup.

    abstract:BACKGROUND AND OBJECTIVES:B subgroups are rare and the genetic analysis reported to date has been limited. MATERIALS AND METHODS:Serological and molecular investigations were performed in blood from a B-subgroup donor. RESULTS:Red cells did not react with anti-B and anti-AB reagents. However, cells absorbed anti-B. R...

    journal_title:Vox sanguinis

    pub_type: 杂志文章

    doi:10.1111/j.1423-0410.2005.00583.x

    authors: Sousa N,Anicchino-Bizzacchi JM,Leite EM,Locatelli MF,Albuquerque D,Costa FF,Barjas-Castro ML

    更新日期:2005-01-01 00:00:00

  • Antibodies to factor VIII in hemophilia A patients.

    abstract::Inhibitor development represents the main complication in the treatment of haemophilia A. The risk of inhibitor formation is in part genetically determined by the type of the underlying factor VIII gene lesion but environmental factors may also play an important role. Due to the lack of efficiency of factor VIII in th...

    journal_title:Vox sanguinis

    pub_type: 杂志文章,评审

    doi:

    authors: Brackmann HH,Schwaab R,Effenberger W,Hess L,Hanfland P,Oldenburg J

    更新日期:2000-01-01 00:00:00

  • Estimation of the infectious viral load required for transfusion-transmitted human T-lymphotropic virus type 1 infection (TT-HTLV-1) and of the effectiveness of leukocyte reduction in preventing TT-HTLV-1.

    abstract:BACKGROUND AND OBJECTIVES:The risk of transfusion-transmitted human T-lymphotropic virus type 1 infection (TT-HTLV-1) after prestorage leucocyte reduction (LR) remains unknown, as the proviral load in the blood component that would cause TT-HTLV-1 is undetermined. On the basis of the distribution of HTLV-1 proviral loa...

    journal_title:Vox sanguinis

    pub_type: 杂志文章

    doi:10.1111/vox.12263

    authors: Sobata R,Matsumoto C,Uchida S,Suzuki Y,Satake M,Tadokoro K

    更新日期:2015-08-01 00:00:00

  • A microtiter modification of granulocyte immunofluorescence.

    abstract::The granulocyte immunofluorescence test (GIFT) is valuable for detecting allogeneic and autologous granulocyte antibodies. However, the original tube technique (macro GIFT) requires 50-100 microliters of serum and 10(6) granulocytes with time-consuming washing steps which cause cell loss. We report a modification (mic...

    journal_title:Vox sanguinis

    pub_type: 杂志文章

    doi:10.1111/j.1423-0410.1985.tb00777.x

    authors: Press C,Kline WE,Clay ME,McCullough J

    更新日期:1985-01-01 00:00:00

  • The ABCB6 mutation p.Arg192Trp is a recessive mutation causing the Lan- blood type.

    abstract:BACKGROUND AND OBJECTIVE:The membrane transporter ABCB6 has recently been shown to carry the high-frequency red-blood-cell (RBC) antigen Lan. All the Lan- individuals genotyped so far have inherited two recessive null mutations in ABCB6. The finding of a family with the Lan- blood type occurring in two successive gener...

    journal_title:Vox sanguinis

    pub_type: 杂志文章

    doi:10.1111/j.1423-0410.2012.01650.x

    authors: Saison C,Helias V,Peyrard T,Merad L,Cartron JP,Arnaud L

    更新日期:2013-02-01 00:00:00

  • Donor selection in Japan: a trial of new criteria with predonation haemoglobin testing.

    abstract:BACKGROUND AND OBJECTIVES:In Japan, eligibility for blood donation depends on blood specific gravity, which does not directly measure blood haemoglobin. Additionally, the criteria are not based on normal values. Therefore, we investigated the feasibility of predonation screening by using actual haemoglobin levels, and ...

    journal_title:Vox sanguinis

    pub_type: 杂志文章

    doi:10.1046/j.0042-9007.2001.00145.x

    authors: Shimoyama R,Nakase T,Kojima S,Tanaka S,Yamamori K,Kaneko C,Ishimaru K,Ikeda H

    更新日期:2002-02-01 00:00:00

  • Reduced risk of transfusion-transmitted HIV in Kenya through centrally co-ordinated blood centres, stringent donor selection and effective p24 antigen-HIV antibody screening.

    abstract:BACKGROUND:Following a 1994 study showing a high rate of transfusion-associated HIV, Kenya implemented WHO blood safety recommendations including: organizing the Kenya National Blood Transfusion Service (NBTS), stringent blood donor selection, and universal screening with fourth-generation p24 antigen and HIV antibody ...

    journal_title:Vox sanguinis

    pub_type: 杂志文章

    doi:10.1111/j.1423-0410.2010.01340.x

    authors: Basavaraju SV,Mwangi J,Nyamongo J,Zeh C,Kimani D,Shiraishi RW,Madoda R,Okonji JA,Sugut W,Ongwae S,Pitman JP,Marum LH

    更新日期:2010-10-01 00:00:00

  • A multicentre evaluation of a new filtration protocol for leucocyte depletion of high-haematocrit red blood cells collected by an automated blood collection system.

    abstract:BACKGROUND AND OBJECTIVES:A multicentre trial was set up to evaluate the performance of a new leucodepletion protocol. MATERIALS AND METHODS:Filtration at high haematocrit was started during collection of red blood cell (RBC) products by apheresis with Trima. SAG-M was added after filtration through the filter. Haemat...

    journal_title:Vox sanguinis

    pub_type: 临床试验,杂志文章,多中心研究

    doi:10.1046/j.1423-0410.2001.00078.x

    authors: Soli M,Blanco L,Riggert J,Martínez-Clavel A,Lucas C,Lunghi M,Belloni M,Wolf C,van Waeg G,Antoon M

    更新日期:2001-01-01 00:00:00

  • The incidence and consequences of cytomegalovirus transmission via blood transfusion to low birth weight, premature infants in north east Scotland.

    abstract::In a 2-year study involving 133 premature low birth weight (less than 1,500 g) infants, the impact of CMV infection via blood transfusion was assessed. 8.4% (7 out of 83) of transfused infants and 10% (7 out of 70) of those exposed to seropositive blood acquired CMV. In those less than 1,250 g the infection rate rose ...

    journal_title:Vox sanguinis

    pub_type: 杂志文章

    doi:10.1111/j.1423-0410.1992.tb01199.x

    authors: Galea G,Urbaniak SJ

    更新日期:1992-01-01 00:00:00

  • Nonradioactive chromium technique for red cell labeling.

    abstract::Following transfusion, red cells labeled with nonradioactive chromium (52Cr) can be detected in the circulation using atomic absorption spectrophotometry. Both in rhesus monkeys and in human subjects, the survival of red cells labeled with 52Cr was found to be insignificantly different from that of cells labeled with ...

    journal_title:Vox sanguinis

    pub_type: 杂志文章

    doi:10.1111/j.1423-0410.1990.tb02091.x

    authors: Sioufi HA,Button LN,Jacobson MS,Kevy SV

    更新日期:1990-01-01 00:00:00

  • Correlation of in vitro platelet quality measurements with in vivo platelet viability in human subjects.

    abstract:BACKGROUND AND OBJECTIVES:Changes in in vitro platelet quality parameters during platelet storage are associated with a decrease of in vivo platelet viability after platelet transfusion. Many attempts have been made to identify the most predictable in vitro parameters for in vivo performance. We used a riboflavin-based...

    journal_title:Vox sanguinis

    pub_type: 杂志文章

    doi:10.1111/j.1423-0410.2006.00761.x

    authors: Goodrich RP,Li J,Pieters H,Crookes R,Roodt J,Heyns Adu P

    更新日期:2006-05-01 00:00:00

  • Extended storage of platelets in SSP platelet additive solution.

    abstract:BACKGROUND AND OBJECTIVES:The aim of this study was to investigate the effects of extended storage of pooled random platelets in SSP+ additive solution (MacoPharma). MATERIALS AND METHODS:Eight buffy coat-derived, pooled, leucoreduced platelet concentrates were prepared in 75% SSP+, 25% plasma using Fresenius/NPBI Com...

    journal_title:Vox sanguinis

    pub_type: 杂志文章

    doi:10.1111/j.1423-0410.2006.00771.x

    authors: Hornsey VS,McColl K,Drummond O,McMillan L,Morrison A,Morrison L,MacGregor IR,Prowse CV

    更新日期:2006-07-01 00:00:00

  • A chromatographic procedure for the purification of human plasma albumin.

    abstract::Albumin is obtainable from human blood plasma by an ion exchange chromatographic procedure in a yield of about 95% and a purity well above Pharmacopoeia requirements. Cryosupernatant, factor IX depleted plasma is precipitated with 12 and 25% w/v polyethylene glycol 4000. The second precipitate is dissolved to 8% w/v p...

    journal_title:Vox sanguinis

    pub_type: 杂志文章

    doi:10.1111/j.1423-0410.1977.tb02240.x

    authors: Curling JM,Berglöf J,Lindquist LO,Eriksson S

    更新日期:1977-08-01 00:00:00

  • Cytomegalovirus (CMV) antibody in male homosexuals: a source for CMV immune globulin.

    abstract::Male homosexuals have been found to have an extraordinarily high prevalence, and high titers, of antibodies to cytomegalovirus (CMV), as determined by complement-fixation and passive hemagglutination tests. These findings support other data suggesting that CMV may be sexually transmitted. The high titers of CMV antibo...

    journal_title:Vox sanguinis

    pub_type: 杂志文章

    doi:10.1111/j.1423-0410.1983.tb01881.x

    authors: Ikram H,Prince AM,Baker LN

    更新日期:1983-03-01 00:00:00

  • Azapropazone-associated antibodies.

    abstract::2 patients on the drug azapropazone developed positive antiglobulin tests. Their sera were each found to contain two distinct antibodies. One was an IgG antibody, probably a drug-dependent autoantibody of the alpha-methyldopa type, but could have been related to the disease. The other was certainly a drug-dependent an...

    journal_title:Vox sanguinis

    pub_type: 杂志文章

    doi:10.1111/j.1423-0410.1984.tb00095.x

    authors: Bird GW,Wingham J,Babb RG,Bacon P,Wood D

    更新日期:1984-01-01 00:00:00

  • The D antigen characteristic of RoHar is a partial D antigen.

    abstract::The results of testing RoHarr cells with panels of monoclonal anti-D suggest that the D antigen (referred to as DHar) encoded by the haplotype (D)c(e) Rh33 is a partial D antigen. IgM monoclonal anti-D are more efficient than IgG monoclonal anti-D in detecting DHar.DHar expresses some but not all of both epD5 and epD6...

    journal_title:Vox sanguinis

    pub_type: 杂志文章

    doi:10.1111/j.1423-0410.1996.tb01317.x

    authors: Wallace M,Lomas-Francis C,Tippett P

    更新日期:1996-01-01 00:00:00

  • PCR-free blood group genotyping using a nanobiosensor.

    abstract:BACKGROUND AND OBJECTIVES:The last two decades have seen major developments in genotyping assays to facilitate the procurement of red blood cell units to alloimmunized patients. To make genotyping faster, simpler and less costly, a nanotechnology approach based on metal/silica fluorescent nanoparticles and a polymer-ba...

    journal_title:Vox sanguinis

    pub_type: 杂志文章

    doi:10.1111/vox.12207

    authors: Brouard D,Ratelle O,Perreault J,Boudreau D,St-Louis M

    更新日期:2015-02-01 00:00:00

  • Identification of kallikrein and FXIa as impurities in therapeutic immunoglobulins: implications for the safety and control of intravenous blood products.

    abstract:BACKGROUND AND OBJECTIVES:The occurrence of thromboembolic events (TEEs) with intravenous immunoglobulin lots (IVIGs) raised the question of the causative agent for these adverse events. We investigated the predominant plasma proteases in 19 IVIG lots from five manufacturers including three lots associated with adverse...

    journal_title:Vox sanguinis

    pub_type: 杂志文章

    doi:10.1111/j.1423-0410.2011.01502.x

    authors: Etscheid M,Breitner-Ruddock S,Gross S,Hunfeld A,Seitz R,Dodt J

    更新日期:2012-01-01 00:00:00

  • Effect of heat on stored red cells during non-flow conditions in a blood-warming device.

    abstract:BACKGROUND AND OBJECTIVES:While blood is flowing within a transfusion-warming device, the blood temperature is usually less than that applied externally. If the flow is temporarily stopped, the temperature can rise above 37 degrees C in some warming devices. We sought to determine whether temperatures near 45 degrees C...

    journal_title:Vox sanguinis

    pub_type: 杂志文章

    doi:10.1159/000031054

    authors: Eastlund T,Van Duren A,Clay ME

    更新日期:1999-01-01 00:00:00

  • The MNSs antigen Ridley (Ria).

    abstract::After more than 20 years the extended pedigree of the only known kindred carrying the low frequency antigen Ria (Ridley) was reinvestigated. It is established that Ria is governed by a gene which is part of or very closely linked to the MNSs locus. Further serological and frequency studies are reported. ...

    journal_title:Vox sanguinis

    pub_type: 杂志文章

    doi:10.1111/j.1423-0410.1984.tb00100.x

    authors: Contreras M,Armitage SE,Stebbing B

    更新日期:1984-01-01 00:00:00

  • The dynamics of clot formation in fresh-frozen plasma.

    abstract:BACKGROUND:Factor VIII (FVIII) levels are used as a quality marker of fresh-frozen plasma (FFP); however, other clotting factors are not routinely measured. METHODS:We assessed additional haemostatic parameters and the dynamics of coagulation using Thrombelastography (TEG) and a thrombin generation test (TGT). FFP was...

    journal_title:Vox sanguinis

    pub_type: 杂志文章

    doi:10.1111/j.1423-0410.2008.01037.x

    authors: Lawrie AS,Cardigan RA,Williamson LM,Machin SJ,Mackie IJ

    更新日期:2008-05-01 00:00:00

  • Genetic variations of CD36 and low platelet CD36 expression - a risk factor for lipemic plasma donation in Taiwanese apheresis donors.

    abstract:BACKGROUND:New CD36 mutations are constantly being identified, although no study has specifically targeted a Taiwanese population. CD36 deficiency can result in dyslipid state and slow clearance of chylomicron. This could be linked to more frequent lipemic donations. STUDY DESIGN AND METHODS:We used flow cytometric me...

    journal_title:Vox sanguinis

    pub_type: 杂志文章

    doi:10.1111/vox.12356

    authors: Lo SC,Lin KH,Hsieh HH,Lin DT,Hu CY

    更新日期:2016-04-01 00:00:00

  • Serratia marcescens strains implicated in adverse transfusion reactions form biofilms in platelet concentrates and demonstrate reduced detection by automated culture.

    abstract:BACKGROUND AND OBJECTIVES:Serratia marcescens is a gram-negative bacterium that has been implicated in adverse transfusion reactions associated with contaminated platelet concentrates. The aim of this study was to investigate whether the ability of S. marcescens to form surface-attached aggregates (biofilms) could acco...

    journal_title:Vox sanguinis

    pub_type: 杂志文章

    doi:10.1111/j.1423-0410.2011.01550.x

    authors: Greco-Stewart VS,Brown EE,Parr C,Kalab M,Jacobs MR,Yomtovian RA,Ramírez-Arcos SM

    更新日期:2012-04-01 00:00:00

  • Complications of blood donation reported to haemovigilance systems: analysis of eleven years of international surveillance.

    abstract:BACKGROUND AND OBJECTIVES:The International Haemovigilance Network collects aggregate data on complications of blood donation from member haemovigilance systems (HVS). We analysed the data collected in 2006-2016 in order to learn from it and consider future improvements. MATERIALS AND METHODS:National HVS entered annu...

    journal_title:Vox sanguinis

    pub_type: 杂志文章

    doi:10.1111/vox.13048

    authors: Wiersum-Osselton JC,Politis C,Richardson C,Goto N,Grouzi E,Marano G,Land KJ

    更新日期:2020-12-05 00:00:00