A microtiter modification of granulocyte immunofluorescence.

Abstract:

:The granulocyte immunofluorescence test (GIFT) is valuable for detecting allogeneic and autologous granulocyte antibodies. However, the original tube technique (macro GIFT) requires 50-100 microliters of serum and 10(6) granulocytes with time-consuming washing steps which cause cell loss. We report a modification (micro GIFT) using microtiter trays which requires only 20 microliters of serum and 2 X 10(5) granulocytes. The modified method conserves reagents, reduces the time required for washing by 67%, decreases cell loss in washing by 74%, reduces the volume of conjugate required by 67%, and compares favorably with the macro GIFT in accuracy, specificity, and sensitivity.

journal_name

Vox Sang

journal_title

Vox sanguinis

authors

Press C,Kline WE,Clay ME,McCullough J

doi

10.1111/j.1423-0410.1985.tb00777.x

subject

Has Abstract

pub_date

1985-01-01 00:00:00

pages

110-3

issue

2

eissn

0042-9007

issn

1423-0410

journal_volume

49

pub_type

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