Abstract:
:The granulocyte immunofluorescence test (GIFT) is valuable for detecting allogeneic and autologous granulocyte antibodies. However, the original tube technique (macro GIFT) requires 50-100 microliters of serum and 10(6) granulocytes with time-consuming washing steps which cause cell loss. We report a modification (micro GIFT) using microtiter trays which requires only 20 microliters of serum and 2 X 10(5) granulocytes. The modified method conserves reagents, reduces the time required for washing by 67%, decreases cell loss in washing by 74%, reduces the volume of conjugate required by 67%, and compares favorably with the macro GIFT in accuracy, specificity, and sensitivity.
journal_name
Vox Sangjournal_title
Vox sanguinisauthors
Press C,Kline WE,Clay ME,McCullough Jdoi
10.1111/j.1423-0410.1985.tb00777.xsubject
Has Abstractpub_date
1985-01-01 00:00:00pages
110-3issue
2eissn
0042-9007issn
1423-0410journal_volume
49pub_type
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