Systematic use of leukocyte-free blood components to prevent alloimmunization and platelet refractoriness in multitransfused children with cancer.

Abstract:

:A major drawback in the use of platelet transfusions is the development of platelet refractoriness that is usually caused by alloimmunization provoked by leukocytes present in the blood products. We evaluated the development of alloimmunization both by serological demonstration of HLA antibodies and by clinical demonstration of platelet refractoriness in 50 multitransfused children who exclusively received filtered, leukocyte-free blood products. The basic diagnoses included acute leukemia, pediatric solid tumors, and severe aplastic anemia. We also present a reference group of 10 similarly multitransfused children, who either deliberately or inadvertently had received nonfiltered blood products on some occasions. In the study group of 50 children, none developed platelet refractoriness. The median corrected increment was 11.1 x 10(9)/l at the time of initial diagnosis, and 10.4 at the time of the study 8 months later (median). None had detectable HLA antibodies in serum. In contrast, in the reference group 3/10 had HLA antibodies in serum, and 1/10 was clearly refractory to random pooled platelets. We conclude that systematic use of leukocyte-free blood components effectively prevents alloimmunization in heavily transfused children with cancer. Leukocyte depletion to the level of < 1 x 10(6) of contaminating leukocytes per unit is sufficient and seems to prevent alloimmunization totally.

journal_name

Vox Sang

journal_title

Vox sanguinis

authors

Saarinen UM,Koskimies S,Myllylä G

doi

10.1111/j.1423-0410.1993.tb02168.x

subject

Has Abstract

pub_date

1993-01-01 00:00:00

pages

286-92

issue

4

eissn

0042-9007

issn

1423-0410

journal_volume

65

pub_type

临床试验,杂志文章
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  • Group O RBCs: where is universal donor blood being used.

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  • Serological characterization of murine monoclonal antibodies directed against acquired B red cells.

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    pub_type: 杂志文章

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  • Platelet-rich plasma and platelet gel preparation using Plateltex.

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    pub_type: 历史文章,杂志文章,评审

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  • Effectiveness of four intravenous IgG preparations in prevention of phagocytosis of anti-Rh-(D)-coated erythrocytes by mononuclear phagocytes.

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  • Reticulocyte count in red-blood-cell units stored in AS-1.

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    pub_type: 杂志文章

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  • Non-HLA lymphocytotoxic antibodies in HIV-seropositive and HIV-seronegative hemophiliacs.

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    更新日期:1988-01-01 00:00:00

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    doi:10.1111/j.1423-0410.1987.tb04925.x

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    更新日期:1987-01-01 00:00:00

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    pub_type: 杂志文章

    doi:10.1046/j.1423-0410.2003.00295.x

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    更新日期:2003-05-01 00:00:00

  • Simultaneous occurrence of anti-F1 and anti-I cold agglutinins in a patient's serum.

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    pub_type: 杂志文章

    doi:10.1111/j.1423-0410.1984.tb01573.x

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    更新日期:1984-01-01 00:00:00

  • Preparation of human immunoglobulin free of plasmin and anticomplement activities.

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    pub_type: 杂志文章

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    更新日期:1977-01-01 00:00:00

  • Blood group ABH antigens on human cord red cells. Number of H antigenic sites and their distribution among different classes of membrane constituents.

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    pub_type: 杂志文章

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    pub_type: 临床试验,杂志文章,随机对照试验

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