HLA-DR: serology versus restriction fragment length polymorphism.

abstract：BACKGROUND AND OBJECTIVES:During the past 10 years several DNA-typing methods have been developed to complement routine serological typing for determination of polymorphisms in the ABO, RH, KEL, JK and FY blood group genes. However, the molecular basis of blood groups can differ widely between ethnic groups. The purpos...

journal_title：Vox sanguinis

authors： Irshaid NM,Ramadan S,Wester ES,Olausson P,Hellberg A,Merza JY,Olsson ML

更新日期：2002-07-01 00:00:00

abstract：:Activated protein C (APC) is a highly specific serine proteinase which functions as an important naturally occurring antithrombotic enzyme. APC also has anti-inflammatory properties. We have developed a large-scale process for the production of APC for therapeutic use starting with cryoprecipitate-poor human plasma. T...

journal_title：Vox sanguinis

authors： Orthner CL,Ralston AH,Gee D,Kent R,Kolen B,McGriff JD,Drohan WN

更新日期：1995-01-01 00:00:00

abstract：:The Am and Bm phenotypes are characterized by weak expression of the A or B antigens, respectively, by red blood cells with a normal expression by the saliva of secretors. Deletion of the regulatory element in the first intron of the ABO gene and disruption of the GATA motif in the element were found to be responsible...

journal_title：Vox sanguinis

authors： Oda A,Isa K,Ogasawara K,Kameyama K,Okuda K,Hirashima M,Ishii H,Kimura K,Matsukura H,Hirayama F,Kawa K

更新日期：2015-05-01 00:00:00

abstract：:A combined immunoassay for the simultaneous detection of antibodies to HIV-1/2 and HTLV-I/II (Bioelisa, Launch Diagnostics) has been evaluated to determine its suitability for routine use in blood screening. 84,222 donations were tested from 76,452 donors. One HIV- and 1 HTLV-1-positive donor were identified. The spec...

journal_title：Vox sanguinis

authors： Flanagan P,McAlpine L,Ramskill SJ,Smith AG,Eglin R,Parry JV,Mortimer PP

更新日期：1995-01-01 00:00:00

abstract：BACKGROUND AND OBJECTIVES:The German Armed Forces Blood Service in Koblenz supplies red blood cell concentrates (RBCs) to military and civilian institutions at home and to field hospitals during peacekeeping operations abroad. During long-distance transport, blood products can be exposed to extreme environmental condit...

journal_title：Vox sanguinis

authors： Klose T,Borchert HH,Pruss A,Roth WK,Bohnen HR,Putzker M

更新日期：2010-07-01 00:00:00

abstract：BACKGROUND AND OBJECTIVES:French Polynesia, where dengue virus (DENV) has been present for a long time, experienced two successive outbreaks of Zika (ZIKV) and chikungunya viruses (CHIKV) between 2013 and 2015. To avoid the transmission of these viruses by transfusion, nucleic acid testing (NAT) has been in place for D...

journal_title：Vox sanguinis

authors： Beau F,Mallet HP,Lastère S,Broult J,Laperche S

更新日期：2020-02-01 00:00:00

abstract：BACKGROUND AND OBJECTIVES:Severe anaemia is an important cause of mortality in developing countries. However, few studies have explored the use of and possibilities for blood transfusion services. The aims of this study are to explore the use of blood transfusion services at a hospital in sub-Saharan Africa and to asse...

journal_title：Vox sanguinis

authors： Bugge HF,Karlsen NC,Oydna E,Rake MM,Wexels N,Bendabenda J,Roald B,Heier HE,Chisuwo L,Jourdan PM

更新日期：2013-01-01 00:00:00

abstract：:Low-dose immune tolerance protocols use doses of factor VIII of less than 50 IU/kg/day to induce immune tolerance. Such protocols are chosen for a variety of reasons including cost, patient acceptability and ease of administration. Early published reports of low-dose protocols appeared in 1981, described small numbers...

journal_title：Vox sanguinis

authors： Sultan Y

更新日期：1999-01-01 00:00:00

abstract：:The following substances were tested for their influence on granulocyte function: 8 sera that contained human granulocyte-specific alloantibodies against the antigens NA1, NA2 and NB1, two HLA antisera, and the monoclonal antibodies W6/32 and CLB-FcR-gran 1. The effects examined included spontaneous and directed migra...

journal_title：Vox sanguinis

authors： Bux J,Dickmann JO,Stockert U,Mueller-Eckhardt C

更新日期：1993-01-01 00:00:00

abstract：:The sensitivity of a combination of various papain methods with normal and low ionic strength (lis) of the serum-cell mixture for the detection of red cell antibodies was tested after varying periods of incubation of the mixture. The mixture was centrifuged before reading when the period of incubation was short. The m...

journal_title：Vox sanguinis

authors： Jørgensen J,Nielsen M,Laugesen J,Stoltenberg S

更新日期：1979-01-01 00:00:00

abstract：BACKGROUND AND OBJECTIVES:Staphylococcus epidermidis is a predominant contaminant of platelet concentrates (PCs), outcompeting other skin flora bacteria such as Staphylococcus capitis. The accumulation-associated protein (Aap), encoded by the aap gene, is involved in formation of bacterial aggregates (biofilms) in S. e...

journal_title：Vox sanguinis

authors： Alabdullatif M,Ramirez-Arcos S

更新日期：2019-01-01 00:00:00

abstract：BACKGROUND AND OBJECTIVES:The low prevalence antigen, Be(a), is produced by a complex that also produces weak c, e and f (ce). We report here the molecular basis associated with Be(a) antigen expression. MATERIALS AND METHODS:Peripheral blood samples from four Be(a+) probands were tested. Haemagglutination, gDNA extra...

journal_title：Vox sanguinis

authors： Hue-Roye K,O'Shea K,Gillett R,Wadsworth LD,Hume H,Barnes J,Kinney J,Hodgins K,Fuchisawa A,Lomas-Francis C,Reid M

更新日期：2010-04-01 00:00:00

abstract：BACKGROUND AND OBJECTIVES:Serologic tests for granulocyte antibodies, i.e., the granulocyte agglutination test and the granulocyte immunofluorescence test, require panels of typed granulocytes that cannot be preserved for more than a few hours. We have developed a new method in which granulocyte antigens, extracted int...

journal_title：Vox sanguinis

authors： Araki N,Nose Y,Kohsaki M,Mito H,Ito K

更新日期：1999-01-01 00:00:00

abstract：:An alternative to blood transfusion, based on oxygen-carrying solutions, has been sought for over a century. The present 'first-generation' haemoglobin-products were based on observations that crosslinking with, for example, glutaraldehyde, overcame subunit dissociation and renal toxicity. Experience with these soluti...

journal_title：Vox sanguinis

authors： Winslow RM

更新日期：2006-08-01 00:00:00

abstract：:Prolonged storage of red blood cells (RBCs) at 4 degrees C results in decreased intracellular ATP levels with diminished posttransfusion survival. Meryman described a preservative medium, exceptional in its capacity to increase these intracellular levels during the first weeks of storage and later in maintaining adequ...

journal_title：Vox sanguinis

authors： Mazor D,Dvilansky A,Meyerstein N

更新日期：1994-01-01 00:00:00

abstract：:A competitive inhibition assay for functional fibronectin (Fn), based on ELISA technology, is described. The assay measures Fn's physiologic ability to bind to denatured collagen (gelatin). Affinity-purified Fn inhibits the binding of alkaline phosphatase coupled Fn to gelatin-coated wells of a microtiter plate in a c...

journal_title：Vox sanguinis

authors： Doran JE,Callaway BD,Reese AC,Wynn JJ,Mansberger AR

更新日期：1983-01-01 00:00:00

abstract：:The human Lewis gene encodes an alpha(1,3/1,4)-fucosyltransferase responsible for synthesis of the Le(a) and a Le(b) antigens. To define the molecular background for non-functional Lewis genes we have sequenced PCR-amplified DNA fragments from two Le(a-b-) individuals. One was homozygously mutated at nucleotides 202(T...

journal_title：Vox sanguinis

authors： Elmgren A,Börjeson C,Svensson L,Rydberg L,Larson G

更新日期：1996-01-01 00:00:00

abstract：:The international media attention to "bad blood" has fueled governmental action in countries around the world. Donor selection criteria continue to expand, focusing most recently on the possibility of exclusion due to intranasal cocaine use and, in the US, due to incarceration for more than 72 hours. As newer methods ...

journal_title：Vox sanguinis

authors： Sazama K

更新日期：1998-01-01 00:00:00

abstract：:In this prospective study, 26 consecutive patients being treated for haematological malignancies receiving standard (i.e. non-leucocyte-depleted) blood components were observed for the development of refractoriness to platelet transfusions. One hundred and sixteen of the 266 (44%) platelet transfusions failed to produ...

journal_title：Vox sanguinis

authors： Doughty HA,Murphy MF,Metcalfe P,Rohatiner AZ,Lister TA,Waters AH

更新日期：1994-01-01 00:00:00

abstract：:Employing standardized IgG-coated 51Cr-labelled human red blood cells (RBC), variation in rosette formation among monolayers obtained from 10 healthy donors was defined, as well as variation among 24 sequential monolayers obtained from one healthy donor and 8 sequential monolayers from a second healthy donor over a 4-...

journal_title：Vox sanguinis

authors： Munn LR,Chaplin H Jr

更新日期：1977-09-01 00:00:00

abstract：:As part of a programme to establish a panel of individuals with high titres of anticytomegalovirus (CMV) IgG for the production of CMV Ig, the CMV serostatus of 139 normal blood donors was determined by indirect haemagglutination assay, enzyme immunoassay, and complement fixation. The indirect haemagglutination assay ...

journal_title：Vox sanguinis

authors： Chung A,Chang W,Leung MK,Ofosu FA,Moore BP

更新日期：1984-01-01 00:00:00

abstract：BACKGROUND AND OBJECTIVES:Since July 1 1999, four laboratories in the Netherlands have been routinely screening plasma minipools for the release of labile blood components utilizing hepatitis C virus nucleic acid amplification technology (HCV NAT). This report describes the performance evaluation of the HCV NAT method ...

journal_title：Vox sanguinis

authors： Cuijpers HT,Molijn MH,Bos HJ,Peeters AP,van der Poel CL,Lelie PN

更新日期：2001-07-01 00:00:00

abstract：BACKGROUND:Immune haemolysis in liver transplant (LT) can occur due to autoantibodies and alloantibodies. The aim of this study was to evaluate the prevalence and risk factors for immune haemolysis in LT. METHODS:A total of 175 consecutive patients were included. Multiorgan recipients were excluded. Samples, from befo...

journal_title：Vox sanguinis

authors： Brunetta DM,de Barros Carlos LM,Da Silva VFP,de Oliveira Alves TM,Macedo ÊS,Coelho GR,Vasconcelos JBM,De Francesco Daher E,Garcia JHP

更新日期：2020-01-01 00:00:00

abstract：BACKGROUND AND OBJECTIVES:Weak ABO variants may escape tests using unlicensed sera. MATERIALS AND METHODS:Prior to transfusion, ABO grouping was performed using an automated system and in-house diluted sera, and manual and bedside test techniques. Genotyping and sequencing were performed using standard methods. RESUL...

journal_title：Vox sanguinis

authors： Pruss A,Heymann GA,Braun J,Kiesewetter HH,Salama A

更新日期：2006-04-01 00:00:00

abstract：:OCTAPLAS is a cell-free standardized blood group specific human coagulation active plasma for transfusion. The viral safety is mainly based on the treatment with solvent/detergent. SD is known to irreversibly inactivate the lipid enveloped viruses including HIV 1 + 2, HBV and HCV and has been recommended both by Europ...

journal_title：Vox sanguinis

authors： Biesert L,Suhartono H

更新日期：1998-01-01 00:00:00

abstract：:A new granulocyte collection method employing discontinuous flow centrifugation and low donor dose of hydroxyethyl starch (HES) is presented. Following the procedure, the increase in serum polysaccharide concentration was 100 mg/dl, about 20% of that seen after standard discontinuous flow leukapheresis. The average ef...

journal_title：Vox sanguinis

authors： Szymanski IO

更新日期：1983-01-01 00:00:00

abstract：:A 24-hours service was organized to study changes in the hemostatic system in surgical patients undergoing massive transfusion for excessive bleeding during operation or in the early postoperative period. Hemostasis tests gave normal results in only 12(7%) of the 172 patients, while in the remaining 160(93%) one or mo...

journal_title：Vox sanguinis

authors： Mannucci PM,Federici AB,Sirchia G

更新日期：1982-03-01 00:00:00

abstract：:The second International Society of Blood Transfusion and International Council for Standardization in Haematology workshop on molecular blood group genotyping was held in 2006. Forty-one laboratories participated. Six samples were distributed: two representing DNA from transfusion-dependent patients for testing for a...

journal_title：Vox sanguinis

authors： Daniels G,van der Schoot CE,Olsson ML

更新日期：2007-07-01 00:00:00

abstract：:Ocular chronic GVHD is efficaciously treated with autologous platelet-derived eye drops. We investigated the cytokine content of eye drops produced using a non-gelified lysate obtained from autologous platelet-rich plasma in six patients with ocular GVHD. In both the responding (n = 4) and the resistant (n = 2) patien...

journal_title：Vox sanguinis

authors： Valentini CG,Nuzzolo ER,Orlando N,Metafuni E,Bianchi M,Chiusolo P,Zini G,Teofili L

更新日期：2016-02-01 00:00:00

abstract：:We report a preliminary study on whether syphilis serology might be reactive in some blood donors at risk for human immunodeficiency virus (HIV) infection. We retrospectively analyzed voluntary blood donations with reactive Treponema pallidum antibody (TPA) tests according to the type of donation, the presence of othe...

journal_title：Vox sanguinis

authors： Ramsey G,Soltis F,Bowman R,McNamee J,Hahn LF,Dixon BW

更新日期：1991-01-01 00:00:00