Abstract:
:A competitive inhibition assay for functional fibronectin (Fn), based on ELISA technology, is described. The assay measures Fn's physiologic ability to bind to denatured collagen (gelatin). Affinity-purified Fn inhibits the binding of alkaline phosphatase coupled Fn to gelatin-coated wells of a microtiter plate in a concentration-dependent manner. The assay range is 50-500 micrograms Fn/ml, which is suitable for the measurement of plasma Fn in both normal and opsonin deficient individuals. It is reproducible over an eightfold dilution of plasma and is resistant to interference by normal plasma proteins. The assay described is quick, quantitative, and reproducible, and satisfies the need for a measure of functional Fn activity in the clinical laboratory.
journal_name
Vox Sangjournal_title
Vox sanguinisauthors
Doran JE,Callaway BD,Reese AC,Wynn JJ,Mansberger ARdoi
10.1111/j.1423-0410.1983.tb01910.xsubject
Has Abstractpub_date
1983-01-01 00:00:00pages
243-51issue
3eissn
0042-9007issn
1423-0410journal_volume
45pub_type
杂志文章相关文献
VOX SANGUINIS文献大全abstract:BACKGROUND AND OBJECTIVES:GB virus C (GBV-C)/hepatitis G virus (HGV) is a recently recognized parenterally and sexually transmitted agent. The prevalence of GBV-C/HGV markers in Canadian blood donors has not been previously studied and was therefore determined. MATERIALS AND METHODS:Blood donors [identity unlinked (IU...
journal_title:Vox sanguinis
pub_type: 杂志文章
doi:10.1159/000056731
更新日期:2000-01-01 00:00:00
abstract::BACKGROUND Whole blood (WB) donation encompasses several periods during which some donors faint. Identification of factors associated with fainting during each period should guide intervention strategies. Reducing faint reactions may reduce donor injuries and disability. METHODS Blood donation was divided into three...
journal_title:Vox sanguinis
pub_type: 杂志文章
doi:10.1111/j.1423-0410.2011.01494.x
更新日期:2011-11-01 00:00:00
abstract::The second International Society of Blood Transfusion and International Council for Standardization in Haematology workshop on molecular blood group genotyping was held in 2006. Forty-one laboratories participated. Six samples were distributed: two representing DNA from transfusion-dependent patients for testing for a...
journal_title:Vox sanguinis
pub_type:
doi:10.1111/j.1423-0410.2007.00926.x
更新日期:2007-07-01 00:00:00
abstract:BACKGROUND AND OBJECTIVES:The low prevalence antigen, Be(a), is produced by a complex that also produces weak c, e and f (ce). We report here the molecular basis associated with Be(a) antigen expression. MATERIALS AND METHODS:Peripheral blood samples from four Be(a+) probands were tested. Haemagglutination, gDNA extra...
journal_title:Vox sanguinis
pub_type: 杂志文章
doi:10.1111/j.1423-0410.2009.01277.x
更新日期:2010-04-01 00:00:00
abstract::Bivalent 7S subunits were obtained from 7 purified IgM human monoclonal anti-D antibodies with 33-63% of the total protein-retaining functional binding activity. The number of sites per red cell recognized by these subunits ranged between 9,400 and 28,500. The values are taken to indicate that a number of different ep...
journal_title:Vox sanguinis
pub_type: 杂志文章
doi:10.1111/j.1423-0410.1990.tb05021.x
更新日期:1990-01-01 00:00:00
abstract:BACKGROUND AND OBJECTIVES:Necrotizing enterocolitis (NEC) is a common and often severe gastrointestinal emergency in newborn infants. While usually affecting (very) premature infants, an association between NEC and haemolytic disease of the foetus and newborn (HDFN) has been suggested. HDFN may be an additional risk fa...
journal_title:Vox sanguinis
pub_type: 杂志文章
doi:10.1111/vox.12862
更新日期:2020-02-01 00:00:00
abstract:BACKGROUND AND OBJECTIVES:Human monoclonal antibodies have been produced against various Rh antigens. We report here the production of another one against Rh C and the first such antibody against Rh CW. MATERIALS AND METHODS:Two heterohybridomas secreting IgG human monoclonal antibodies against Rh CW and Rh C (MS-353 ...
journal_title:Vox sanguinis
pub_type: 杂志文章
doi:10.1046/j.1423-0410.1997.7330174.x
更新日期:1997-01-01 00:00:00
abstract:BACKGROUND:The rationale for using topical platelet gel therapy is to provide the healing tissues with concentrated platelet-derived factors. Several systems are available to prepare platelet-rich plasma (PRP) and from these, the platelet gel. These systems produce two- to six-fold platelet and growth factor-enriched c...
journal_title:Vox sanguinis
pub_type: 杂志文章
doi:10.1111/j.1423-0410.2009.01188.x
更新日期:2009-08-01 00:00:00
abstract:BACKGROUND AND OBJECTIVES:Pr1,2,3, PrM, Sa and Sia-l1, -b1, -lb1 are sialic acid (NeuNAc)-dependent antigens recognized by human cold agglutinins. Pr and Sa antigens are the O-glycans of glycophorins containing alpha 2,3NeuNAc (to galactose) and/or alpha 2,6NeuNAc (to galactosamine). The antigens of the Sia-l1, -b1, -l...
journal_title:Vox sanguinis
pub_type: 杂志文章
doi:
更新日期:1998-01-01 00:00:00
abstract::The domain specificity of anti-factor VIII (FVIII) inhibitor antibodies was determined in assays using FVIII domains generated by thrombin cleavage or expressed as recombinant polypeptides to neutralise the inhibitor. The results revealed the existence of three major types of inhibitors, and various combinations of th...
journal_title:Vox sanguinis
pub_type: 杂志文章,评审
doi:10.1159/000056708
更新日期:1999-01-01 00:00:00
abstract:BACKGROUND AND OBJECTIVES:Variant RHD genes associated with the weak D phenotype can result in complete or partial D-epitope expression on the red cell. This study examines the genetic classification in Australian blood donors with a weak D phenotype and correlates RHD variants associated with the weak D phenotype agai...
journal_title:Vox sanguinis
pub_type: 杂志文章
doi:10.1111/vox.12488
更新日期:2017-04-01 00:00:00
abstract::Neonatal alloimmune thrombocytopenia (NAIT) is usually induced by platelet-specific antibodies against HPA-1a (Zwa) or HPA-5b (Bra). Recently, low-frequency alloantigens on the platelet glycoprotein (GP) IIb/IIIa complex have been discovered as a cause for NAIT. In this report, a new low-frequency platelet-specific al...
journal_title:Vox sanguinis
pub_type: 杂志文章
doi:10.1111/j.1423-0410.1995.tb02604.x
更新日期:1995-01-01 00:00:00
abstract:BACKGROUND AND OBJECTIVES:A 2-year-old female with neuroblastoma needed In(b-), E- red blood cells (RBCs). No units were available at the blood centre (BC) nor in the rare donor programme member's inventories. BC's Immunohematology Reference Laboratory (IRL) and its marketing department concentrated on recruiting and t...
journal_title:Vox sanguinis
pub_type: 杂志文章
doi:10.1111/vox.13053
更新日期:2020-12-21 00:00:00
abstract::Prothrombin complex concentrates prepared for clinical use are reviewed with emphasis on frequently observed adverse reactions such as viral hepatitis and thromboembolic complications. Preparation procedures and quality control are described briefly, and the use of these concentrates in the treatment of hemophilia A p...
journal_title:Vox sanguinis
pub_type: 杂志文章,评审
doi:10.1111/j.1423-0410.1981.tb01048.x
更新日期:1981-11-01 00:00:00
abstract::The commercial immune serum globulin (ISG) product is suitable for use in passive immunization only by intramuscular administration; intravenous use results in vasomotor side reactions. We report the functional characterization of a chemically modified ISG (MISG) that is essentially free of nonspecific complement-bind...
journal_title:Vox sanguinis
pub_type: 杂志文章
doi:10.1111/j.1423-0410.1981.tb00726.x
更新日期:1981-01-01 00:00:00
abstract:OBJECTIVES:To determine whether the attitudes, beliefs and motivations of blood donors evolve over time. MATERIALS AND METHODS:Using questionnaires a 7-year longitudinal study of a cohort of 126 donors was performed to gather their sociodemographic characteristics and their attitudes, beliefs and motivations relating ...
journal_title:Vox sanguinis
pub_type: 杂志文章
doi:
更新日期:1998-01-01 00:00:00
abstract:BACKGROUND AND OBJECTIVES:Although most attention has been paid to viral infections as a complication of transfusion, some parasitic infections are readily transmissible and generate a heavy burden of disease, particularly in the developing world. Additionally, bacterial infection as a result of transfusion is the most...
journal_title:Vox sanguinis
pub_type: 杂志文章,评审
doi:
更新日期:2000-01-01 00:00:00
abstract::We have used the Optical Character Recognition system B (OCR-B) for automatic identification of blood samples on the Groupamatic 360 and in connection with computerized donor file management and control of blood product processing. For the Groupamatic system a device was constructed that lifts that samples, and during...
journal_title:Vox sanguinis
pub_type: 杂志文章
doi:
更新日期:1981-03-01 00:00:00
abstract::BS II lectin, a second hemagglutinin isolated from Bandeiraea simplicifolia seeds, agglutinated the red cells from 11 patients manifesting the 'acquired B' antigen phenomenon. The results of serological studies indicate that this lectin has specificity for the type III polyagglutinable receptors rather than for the 'B...
journal_title:Vox sanguinis
pub_type: 杂志文章
doi:10.1111/j.1423-0410.1977.tb04469.x
更新日期:1977-10-01 00:00:00
abstract::Mild as well as strong periodate oxidation of isolated erythrocyte N and M glycoproteins and glycopeptides gave extensive to complete destruction of N-specificities as measured with Vicia graminea extracts and of N- as well as M- activities determined with all but 1 of 8 animal anti-N and 13 anti-M sera. Results with ...
journal_title:Vox sanguinis
pub_type: 杂志文章
doi:10.1111/j.1423-0410.1978.tb02931.x
更新日期:1978-01-01 00:00:00
abstract::The effect on cryoprecipitate factor VIII recovery of three imortant variables has been investigated. The design of the study was such as to eliminate, for the purpose of internal comparisons, spurious effects such as those due to the variation in factor VIII levels in donor plasma. The choice of anticoagulant was sho...
journal_title:Vox sanguinis
pub_type: 杂志文章
doi:10.1111/j.1423-0410.1980.tb02358.x
更新日期:1980-01-01 00:00:00
abstract:BACKGROUND AND OBJECTIVES:Transfusion of fresh-frozen plasma is still a pillar in emergency medicine for using to prevent dilutional coagulopathy or disseminated intravascular coagulation after severe blood loss, but thawing procedures can delay its availability. On the other hand, the wastage of plasma, once thawed an...
journal_title:Vox sanguinis
pub_type: 杂志文章
doi:10.1111/j.1423-0410.2004.00552.x
更新日期:2004-10-01 00:00:00
abstract:BACKGROUND AND OBJECTIVES:Comparison of two models for estimating residual transfusion transmission risk by NAT screened window period (WP) donations in South African repeat donors gave identical results for HIV but not for HBV. In order to understand discrepant HBV modelling outcomes, the values of input parameters in...
journal_title:Vox sanguinis
pub_type: 杂志文章,评审
doi:10.1111/vox.12889
更新日期:2020-04-01 00:00:00
abstract:BACKGROUND AND OBJECTIVES:Severe anaemia is an important cause of mortality in developing countries. However, few studies have explored the use of and possibilities for blood transfusion services. The aims of this study are to explore the use of blood transfusion services at a hospital in sub-Saharan Africa and to asse...
journal_title:Vox sanguinis
pub_type: 杂志文章
doi:10.1111/j.1423-0410.2012.01628.x
更新日期:2013-01-01 00:00:00
abstract::Manual plasmapheresis is done worldwide on both paid donors, and to a much smaller extent on volunteer donors in order to generate source plasma to be fractionated into albumin, clotting factor concentrates, and gamma globulin. Automated technology has now been developed utilizing both centrifugal, and membrane separa...
journal_title:Vox sanguinis
pub_type: 杂志文章
doi:10.1111/j.1423-0410.1986.tb01992.x
更新日期:1986-01-01 00:00:00
abstract::Monocyte-erythrocyte interaction in patients with autoimmune haemolytic anaemia (AIHA) was assessed by phagocytosis and rosette assays. In most patients, a relationship was observed between haemolysis and the phagocytosis of their own erythrocytes by allogenic peripheral monocytes. An evaluation of the number of immun...
journal_title:Vox sanguinis
pub_type: 杂志文章
doi:10.1111/j.1423-0410.1987.tb03030.x
更新日期:1987-01-01 00:00:00
abstract::We report a preliminary study on whether syphilis serology might be reactive in some blood donors at risk for human immunodeficiency virus (HIV) infection. We retrospectively analyzed voluntary blood donations with reactive Treponema pallidum antibody (TPA) tests according to the type of donation, the presence of othe...
journal_title:Vox sanguinis
pub_type: 杂志文章
doi:10.1111/j.1423-0410.1991.tb00895.x
更新日期:1991-01-01 00:00:00
abstract:BACKGROUND AND OBJECTIVES:In Japan, eligibility for blood donation depends on blood specific gravity, which does not directly measure blood haemoglobin. Additionally, the criteria are not based on normal values. Therefore, we investigated the feasibility of predonation screening by using actual haemoglobin levels, and ...
journal_title:Vox sanguinis
pub_type: 杂志文章
doi:10.1046/j.0042-9007.2001.00145.x
更新日期:2002-02-01 00:00:00
abstract::Platelet concentrates were prepared in plastic packs of polyvinyl chloride with tri(2-ethylhexyl) trimellitate as plasticizer. They were stored, with gentle shaking, at room temperature for periods up to 7 days before labelling with isotope and reinfusing. In vivo survival studies, platelet counts, pH and electron mic...
journal_title:Vox sanguinis
pub_type: 杂志文章
doi:10.1111/j.1423-0410.1982.tb00016.x
更新日期:1982-01-01 00:00:00
abstract::Blood plasma and factor VIII concentrate have been fractionated by continuous free film electrophoresis in an apparatus which was designed to overcome the problems of overheating usually inherent in this technique. Results show that complete separation of plasma proteins is unlikely to be obtained by a single passage ...
journal_title:Vox sanguinis
pub_type: 杂志文章
doi:10.1111/j.1423-0410.1983.tb01877.x
更新日期:1983-03-01 00:00:00