A competitive inhibition assay for gelatin binding fibronectin.

Abstract:

:A competitive inhibition assay for functional fibronectin (Fn), based on ELISA technology, is described. The assay measures Fn's physiologic ability to bind to denatured collagen (gelatin). Affinity-purified Fn inhibits the binding of alkaline phosphatase coupled Fn to gelatin-coated wells of a microtiter plate in a concentration-dependent manner. The assay range is 50-500 micrograms Fn/ml, which is suitable for the measurement of plasma Fn in both normal and opsonin deficient individuals. It is reproducible over an eightfold dilution of plasma and is resistant to interference by normal plasma proteins. The assay described is quick, quantitative, and reproducible, and satisfies the need for a measure of functional Fn activity in the clinical laboratory.

journal_name

Vox Sang

journal_title

Vox sanguinis

authors

Doran JE,Callaway BD,Reese AC,Wynn JJ,Mansberger AR

doi

10.1111/j.1423-0410.1983.tb01910.x

subject

Has Abstract

pub_date

1983-01-01 00:00:00

pages

243-51

issue

3

eissn

0042-9007

issn

1423-0410

journal_volume

45

pub_type

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