Partial separation of blood clotting factors, albumin, and IgG by continuous free film electrophoresis.

Abstract:

:Blood plasma and factor VIII concentrate have been fractionated by continuous free film electrophoresis in an apparatus which was designed to overcome the problems of overheating usually inherent in this technique. Results show that complete separation of plasma proteins is unlikely to be obtained by a single passage through this apparatus. However, a useful degree of fractionation may be obtained with certain proteins, for example fibrinogen and IgG. It should be possible to concentrate blood clotting factors if the electrophoresis is used in combination with a conventional separation procedure such as adsorption on aluminium hydroxide. The advantage of the electrophoretic technique is that it is a continuous process using the minimum of extraneous chemicals. The disadvantage is that it introduces a 40-fold dilution into the fractionation.

journal_name

Vox Sang

journal_title

Vox sanguinis

authors

Austen DE,Cartwright T,Dickerson CH

doi

10.1111/j.1423-0410.1983.tb01877.x

subject

Has Abstract

pub_date

1983-03-01 00:00:00

pages

151-5

issue

3

eissn

0042-9007

issn

1423-0410

journal_volume

44

pub_type

杂志文章
  • False reactions in radioimmunoassay for viral hepatitis B markers in patients suffering from coagulation disorders.

    abstract::Simultaneous occurrence of hepatitis B surface antigen (HBsAg) and antibodies to HBsAg (anti-HBs) has been frequently described in hemophiliac patients. During our working procedures, this phenomenon was also observed. Interference of coagulation disorders with radioimmunoassay (RIA) used in the detection of viral hep...

    journal_title:Vox sanguinis

    pub_type: 杂志文章

    doi:10.1111/j.1423-0410.1982.tb01088.x

    authors: Grangeot-Keros L,Lambert T,Dubreuil P,Briantais MJ,Pillot J

    更新日期:1982-03-01 00:00:00

  • Effect of rate and delay of cooling during initial cooling process: in vitro effect on red cells.

    abstract:BACKGROUND:Whole blood is stored at room temperature (RT) until processing into components. After separation and filtration, the RCC has to be cooled from RT to +2 - 6°C. Different start times of the cooling process and different cooling rates can be encountered in daily routine. The effect of these parameters of the i...

    journal_title:Vox sanguinis

    pub_type: 杂志文章

    doi:10.1111/j.1423-0410.2010.01458.x

    authors: Bontekoe IJ,van der Meer PF,de Korte D

    更新日期:2011-07-01 00:00:00

  • Molecular basis of Tn-polyagglutinability.

    abstract::Spectrophotometric and gas-liquid chromatographic analyses on the carbohydrate moiety of tryptic erythrocyte glycopeptides from persons with Tn-syndrome reveal a selective lowering of the galactose and sialic acid content, the degree being dependent on the percentage of polyagglutinable cells. Alkaline borohydride spe...

    journal_title:Vox sanguinis

    pub_type: 杂志文章

    doi:10.1111/j.1423-0410.1975.tb00475.x

    authors: Dahr W,Uhlenbruck G,Gunson HH,Van Der Hart M

    更新日期:1975-01-01 00:00:00

  • Estimated risk of transmission of hepatitis C virus by blood transfusion.

    abstract:OBJECTIVE:The risk of transmitting hepatitis C (HCV) by transfusion of anti-HCV-negative screened blood was estimated for the blood donor population of Baden-Württemberg (southwestern Germany). METHODS:The data from the blood donors screened for anti-HCV and for HBsAg during 1990-1995 were analyzed. RESULTS:The preva...

    journal_title:Vox sanguinis

    pub_type: 杂志文章,多中心研究

    doi:

    authors: Koerner K,Cardoso M,Dengler T,Kerowgan M,Kubanek B

    更新日期:1998-01-01 00:00:00

  • Prothrombin complex concentrate for emergent reversal of warfarin: an international survey of hospital protocols.

    abstract::For emergent warfarin reversal, four-factor prothrombin complex concentrates (4FPCCs) are recommended by many international guidelines. We surveyed international clinical sites including members of the Biomedical Excellence for Safer Transfusion (BEST) Collaborative. Most sites have emergent warfarin reversal protocol...

    journal_title:Vox sanguinis

    pub_type: 杂志文章

    doi:10.1111/vox.12539

    authors: Gorlin J,Kinney S,Fung MK,Tinmouth A,BEST Collaborative.

    更新日期:2017-08-01 00:00:00

  • Incidence of hepatitis associated antigen HAA and homologous antibody in patients with rheumatoid arthritis.

    abstract::The authors have studied the incidence of hepatitis associated antigen (HAA) and the homologous antibody in sera of patients with rheumatoid arthritis on the basis of (1) arthritis sometimes associated with viral hepatitis, (2) the possible infectious etiology of rheumatoid arthritis, and (3) observation on the possib...

    journal_title:Vox sanguinis

    pub_type: 杂志文章

    doi:10.1111/j.1423-0410.1975.tb02735.x

    authors: Marcolongo R,Debolini A

    更新日期:1975-01-01 00:00:00

  • The D antigen characteristic of RoHar is a partial D antigen.

    abstract::The results of testing RoHarr cells with panels of monoclonal anti-D suggest that the D antigen (referred to as DHar) encoded by the haplotype (D)c(e) Rh33 is a partial D antigen. IgM monoclonal anti-D are more efficient than IgG monoclonal anti-D in detecting DHar.DHar expresses some but not all of both epD5 and epD6...

    journal_title:Vox sanguinis

    pub_type: 杂志文章

    doi:10.1111/j.1423-0410.1996.tb01317.x

    authors: Wallace M,Lomas-Francis C,Tippett P

    更新日期:1996-01-01 00:00:00

  • Donor follow-up of influenza vaccine-related multiple viral enzyme immunoassay reactivity.

    abstract::False-positive enzyme immunoassay (EIA) tests in blood donors receiving influenza vaccine were first reported in 1991. We conducted follow-up testing for 6 months of those donors with multiply reactive, but unconfirmed EIA (at least 2 positives in anti-HCV-1.0, anti-HIV-1, and anti-HTLV-I assays) with a history of rec...

    journal_title:Vox sanguinis

    pub_type: 杂志文章

    doi:10.1111/j.1423-0410.1994.tb01658.x

    authors: Arnold NL,Slade BA,Jones MM,Popovsky MA

    更新日期:1994-01-01 00:00:00

  • Transmission of parasites by blood transfusion.

    abstract::A number of parasitic diseases are known, or suspected to be transmitted by blood transfusion. Of greatest concern are malaria and Chagas' disease, but babesia, leishmania and toxoplasmosis also offer risk in particular locations or circumstances. Some of these parasites may be imported into non-endemic areas as a res...

    journal_title:Vox sanguinis

    pub_type: 杂志文章,评审

    doi:10.1111/j.1423-0410.1998.tb05415.x

    authors: Dodd RY

    更新日期:1998-01-01 00:00:00

  • KEL*02 alleles with alterations in and around exon 8 in individuals with apparent KEL:1,-2 phenotypes.

    abstract:BACKGROUND AND OBJECTIVES:Antibodies to antigens in the Kell blood group system, especially anti-KEL1, are involved in both haemolytic disease of the newborn and foetus and haemolytic transfusion reactions. Correct typing results are important and discrepancies between serologic and genetic typing must be resolved. Her...

    journal_title:Vox sanguinis

    pub_type: 杂志文章

    doi:10.1111/j.1423-0410.2010.01334.x

    authors: Wester ES,Steffensen R,Ligthart PC,Vad J,de Haas M,Storry JR,Olsson ML

    更新日期:2010-08-01 00:00:00

  • Monoclonal antibody recognizing a unique Rh-related specificity.

    abstract::A mouse IgG1 monoclonal antibody (MAb) UMRh, was prepared by immunizing Balb/c mice with the Jurkat T cell acute lymphoblastic leukemia (T-ALL) cell line. The MAb UMRh is directed against a widely distributed Rh-related cell surface antigen, present on red blood cells (RBCs) expressing the more common Rh phenotypes. T...

    journal_title:Vox sanguinis

    pub_type: 杂志文章

    doi:10.1111/j.1423-0410.1993.tb03061.x

    authors: Poss MT,Swanson JL,Telen MJ,Lasky LC,Vallera DA

    更新日期:1993-01-01 00:00:00

  • Transferrin binding by peripheral blood mononuclear cells in human lymphomas, myelomas and leukemias.

    abstract::Significantly more peripheral blood mononuclear cells (PBM) from 39 patients with lymphoma (p less than 0.001), myeloma (p less than 0.001), or leukemia (p less than 0.001-0.025) bind transferrin (Trf) than those of 23 normals. That Trf was bound to a receptor on these cells was shown by the inhibition of these cells ...

    journal_title:Vox sanguinis

    pub_type: 杂志文章

    doi:10.1111/j.1423-0410.1984.tb00078.x

    authors: Yeh CJ,Taylor CG,Faulk WP

    更新日期:1984-01-01 00:00:00

  • Expression of genetic markers of erythrocyte immunoglobulin G autoantibodies in autoimmune hemolytic anemia.

    abstract::The Gm allotype constitution of the autoantibody molecules and the serum Gm phenotypes were determined in 19 patients with autiommune hemolytic anemia of IgG type. The results showed that the known heterogeneity of the antierythrocyte autoantibodies in this disease concerns not only the immunoglobulin class and the is...

    journal_title:Vox sanguinis

    pub_type: 杂志文章

    doi:10.1111/j.1423-0410.1976.tb02208.x

    authors: Le Petit JC,Rivat L,François N,Ropartz C,Brizard CP

    更新日期:1976-01-01 00:00:00

  • Prevalence and laboratorial determinants of the clinical relevance of antibodies of undetermined specificity.

    abstract:BACKGROUND AND OBJECTIVES:Antibodies of unknown specificity (AUS) are frequently identified in the pre-transfusion testing. These antibodies can be insignificant or potentially cause post-transfusion haemolysis. Information about the prevalence of clinically relevant AUS is still lacking. Our aim was to predict the pot...

    journal_title:Vox sanguinis

    pub_type: 杂志文章

    doi:10.1111/vox.12797

    authors: de Albuquerque da Veiga Conrado MC,Cardoso RA,Dezan MR,Oliveira VB,Neto ADC,Ziza KC,Krieger JE,Pereira AC,Sabino EC,Rocha V,Mendrone-Júnior A,Dinardo CL

    更新日期:2019-08-01 00:00:00

  • Blood transfusion in sickle cell disease.

    abstract::Blood transfusion is used as a life-saving and prophylactic treatment in sickle cell disease. Despite the many complications associated with its use, few randomised controlled trials and careful research studies have been performed to fully define its role. This subjects is, therefore, discussed in the context of the ...

    journal_title:Vox sanguinis

    pub_type: 杂志文章,评审

    doi:10.1111/j.1423-0410.1995.tb03916.x

    authors: Davies SC,Olatunji PO

    更新日期:1995-01-01 00:00:00

  • Plasmodium falciparum malaria and the immunogenetics of ABO, HLA, and CD36 (platelet glycoprotein IV).

    abstract::Plasmodium falciparum malaria has long been a killer of the young, and has selected for polymorphisms affecting not only erythrocytes, but the immunogenetics of three histocompatibility systems: ABO, human leukocyte antigen (HLA), and CD36. The ABO system is important because the original allele, encoding glycosylatio...

    journal_title:Vox sanguinis

    pub_type: 杂志文章,评审

    doi:10.1111/j.1423-0410.2010.01429.x

    authors: Cserti-Gazdewich CM,Mayr WR,Dzik WH

    更新日期:2011-01-01 00:00:00

  • A Swiss family showing independent segregation of the Lutheran and Swann genes.

    abstract::A family is reported in which the parents' mating is of the 'double back-cross' type with respect to the Sw, Lu and Se genes. The inheritance of these genes in the children shows, with reference to a possible linkage between Sw and the two other loci, four non-recombinants and three recombinants (or vice versa). The i...

    journal_title:Vox sanguinis

    pub_type: 杂志文章

    doi:10.1111/j.1423-0410.1976.tb02221.x

    authors: Metaxas MN,Metaxas-Buehler M

    更新日期:1976-01-01 00:00:00

  • Rosette formation by sensitized human red cells- effects of source of peripheral leukocyte monolayers.

    abstract::Employing standardized IgG-coated 51Cr-labelled human red blood cells (RBC), variation in rosette formation among monolayers obtained from 10 healthy donors was defined, as well as variation among 24 sequential monolayers obtained from one healthy donor and 8 sequential monolayers from a second healthy donor over a 4-...

    journal_title:Vox sanguinis

    pub_type: 杂志文章

    doi:10.1111/j.1423-0410.1977.tb02245.x

    authors: Munn LR,Chaplin H Jr

    更新日期:1977-09-01 00:00:00

  • Inactivation of hepatitis A virus by pasteurization and elimination of picornaviruses during manufacture of factor VIII concentrate.

    abstract::Hepatitis A virus (HAV) infections have been reported among hemophiliacs who received factor VIII concentrates which had been purified by ion-exchange chromatography and treated by the solvent detergent (SD) method. Since the virus inactivation procedure of our manufacturing process is heat treatment of the stabilized...

    journal_title:Vox sanguinis

    pub_type: 杂志文章

    doi:

    authors: Hilfenhaus J,Nowak T

    更新日期:1994-01-01 00:00:00

  • A family with allo-immune neonatal neutropenia: group-specific pathogenicity of maternal antibodies.

    abstract::A family is presented, in which the mother developed strong neutrophil granulocyte-specific antibodies with the specificity anti-NA. The first and third children, both NA1-positive, suffered from severe transient neonatal neutropenia, but the second child, NA1-negative, did not. Anti-NA1 antibodies were detectable in ...

    journal_title:Vox sanguinis

    pub_type: 杂志文章

    doi:10.1111/j.1423-0410.1979.tb04390.x

    authors: Verheugt FW,van Noord-Bokhorst JC,von dem Borne AE,Engelfriet CP

    更新日期:1979-01-01 00:00:00

  • Thrombocytopenia at birth in neonates with red cell alloimmune haemolytic disease.

    abstract:OBJECTIVE:To evaluate the incidence and severity of and risk factors for thrombocytopenia at birth in neonates with red cell alloimmunization. STUDY DESIGN:All neonates with haemolytic disease of the foetus/newborn (HDFN) due to red cell alloimmunization admitted to our centre between January 2000 and September 2010 w...

    journal_title:Vox sanguinis

    pub_type: 杂志文章,随机对照试验

    doi:10.1111/j.1423-0410.2011.01539.x

    authors: Rath ME,Smits-Wintjens VE,Oepkes D,van Zwet EW,van Kamp IL,Brand A,Walther FJ,Lopriore E

    更新日期:2012-04-01 00:00:00

  • Longer storage of red blood cells is associated with increased in vitro erythrophagocytosis.

    abstract:BACKGROUND AND OBJECTIVES:Refrigerated storage of red blood cells (RBCs) induces numerous changes that may target the cells for erythrophagocytosis following transfusion. The influence of storage upon the phagocytosis of unseparated and fractionated young and old stored RBCs was investigated using two in vitro quantita...

    journal_title:Vox sanguinis

    pub_type: 杂志文章

    doi:10.1111/vox.12095

    authors: Veale MF,Healey G,Sparrow RL

    更新日期:2014-04-01 00:00:00

  • Sensitivity of assays for the detection of HPA-1a antibodies: results of an international workshop demonstrating the impact of cation chelation from integrin αIIbβ3 on three widely used assays.

    abstract:BACKGROUND AND OBJECTIVES:HPA-1a antibodies account for 70-80% of cases of fetal-neonatal alloimmune thrombocytopenia (FNAIT) in Caucasians. However, numerous workshops have demonstrated variability in their detection. We recently showed that exposure of αIIbβ3 to ethylene diamine tetraacetic acid (EDTA) affected bindi...

    journal_title:Vox sanguinis

    pub_type: 杂志文章

    doi:10.1111/vox.12043

    authors: Allen DL,Metcalfe P,Kaplan C,Kekomaki R,de Haas M,Yusuf R,Ouwehand WH

    更新日期:2013-08-01 00:00:00

  • The effect of previous blood transfusion on lymphocyte subsets and natural killer cell function in patients with colorectal cancer.

    abstract::To elucidate the possible influence of previous blood transfusion on immune functions, the transfusion history of 153 patients admitted to hospital for elective colorectal surgery was correlated with lymphocyte subsets and natural killer (NK) cell function. The subsets determined were CD2, CD3, CD4, CD8, CD16, CD20, C...

    journal_title:Vox sanguinis

    pub_type: 杂志文章

    doi:10.1111/j.1423-0410.1994.tb05035.x

    authors: Mathiesen O,Lund L,Brodthagen U,Gandrup P,Grunnet N,Balslev I,Jersild C

    更新日期:1994-01-01 00:00:00

  • Development of white blood cell fragments, during the preparation and storage of platelet concentrates, as measured by using real-time polymerase chain reaction.

    abstract:BACKGROUND AND OBJECTIVES:White blood cell (WBC) fragments may cause human leucocyte antigen (HLA) immunization in recipients. We investigated the occurrence and production of WBC fragments in platelet concentrates (PCs) and plasma units, during storage and filtration, by using real-time polymerase chain reaction (PCR)...

    journal_title:Vox sanguinis

    pub_type: 杂志文章

    doi:10.1111/j.1423-0410.2004.00576.x

    authors: Dijkstra-Tiekstra MJ,van der Schoot CE,Pietersz RN,Huijgens PC,van der Meer PF,Reesink HW

    更新日期:2004-11-01 00:00:00

  • Genotype frequencies of the human platelet antigen, Ca/Tu, in Japanese, determined by a PCR-RFLP method.

    abstract::Recently, the polymorphism of a new human platelet antigen, Ca/Tu, was shown to be derived from a G-A nucleotide substitution at base 1564 of GPIIIa cDNA, which leads to a single amino acid difference, Arg/Gln at amino acid 489 of GPIIIa. We developed a PCR-RFLP method to determine the genotypes of Ca/Tu and their fre...

    journal_title:Vox sanguinis

    pub_type: 杂志文章

    doi:10.1111/j.1423-0410.1996.tb00995.x

    authors: Tanaka S,Taniue A,Nagao N,Tomita T,Ohnoki S,Shibata H,Okubo Y,Yamaguchi H,Shibata Y

    更新日期:1996-01-01 00:00:00

  • An autoantibody with activity dependent on red cell age in the serum of a patient with autoimmune haemolytic anaemia and a negative direct antiglobulin test.

    abstract::A red cell IgM autoantibody with anti-e specificity was identified in the serum of a rhesus-negative (rr) patient presenting with haemolytic anaemia and a negative direct antiglobulin test. The autoantibody strongly agglutinated normal allogeneic rhesus-negative (rr) red cells in saline at 37 degrees C but had only we...

    journal_title:Vox sanguinis

    pub_type: 杂志文章

    doi:10.1111/j.1423-0410.1987.tb02993.x

    authors: Herron R,Clark M,Smith DS

    更新日期:1987-01-01 00:00:00

  • Necrotizing enterocolitis in haemolytic disease of the newborn: a retrospective cohort study.

    abstract:BACKGROUND AND OBJECTIVES:Necrotizing enterocolitis (NEC) is a common and often severe gastrointestinal emergency in newborn infants. While usually affecting (very) premature infants, an association between NEC and haemolytic disease of the foetus and newborn (HDFN) has been suggested. HDFN may be an additional risk fa...

    journal_title:Vox sanguinis

    pub_type: 杂志文章

    doi:10.1111/vox.12862

    authors: Ree IMC,de Grauw AM,Bekker V,de Haas M,Te Pas AB,Oepkes D,Middeldorp AJM,Lopriore E

    更新日期:2020-02-01 00:00:00

  • Novel polymorphisms in exons 6 and 7 of A/B alleles detected by polymerase chain reaction-single strand conformation polymorphism.

    abstract:BACKGROUND AND OBJECTIVES:The ABO blood group system is the most important blood group system in transfusion medicine. In addition to the major A, B and O alleles, many rare alleles have been defined. Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) and analysis by PCR sequence specific pri...

    journal_title:Vox sanguinis

    pub_type: 杂志文章

    doi:10.1111/j.1423-0410.2005.00729.x

    authors: Chen YJ,Chen PS,Liu HM,Lyou JY,Hu HY,Lin JS,Tzeng CH

    更新日期:2006-02-01 00:00:00

  • A simplified method for the preparation of immune-serum globulin.

    abstract::This report describes the recovery of highly purified immune-serum globulin (ISG) from the 42% ethanol precipitate fraction (42% P) generated during the first step of albumin purification. The method consists of three purification steps: (1) reprecipitation of the 42% P at 20% ethanol, pH 7.2; (2) solubilization of IS...

    journal_title:Vox sanguinis

    pub_type: 杂志文章

    doi:10.1111/j.1423-0410.1981.tb00706.x

    authors: Hao YL,Wickerhauser M

    更新日期:1981-04-01 00:00:00