Longer storage of red blood cells is associated with increased in vitro erythrophagocytosis.

Abstract:

BACKGROUND AND OBJECTIVES:Refrigerated storage of red blood cells (RBCs) induces numerous changes that may target the cells for erythrophagocytosis following transfusion. The influence of storage upon the phagocytosis of unseparated and fractionated young and old stored RBCs was investigated using two in vitro quantitative phagocytosis assays. MATERIALS AND METHODS:Leucocyte-depleted RBC units were sampled at day 1 or 42 of storage. Young and old RBCs were fractionated at day 1 by density centrifugation and stored in paediatric packs for up to 42 days. RBCs were labelled with the fluorescent dye PKH26 and incubated with the human monocytic cell line THP-1. Erythrophagocytosis was quantified by flow cytometry and plate fluorometric assays. RESULTS:A higher proportion of THP-1 cells phagocytosed RBCs stored for 42 days compared with 1 day (41% and 24% respectively; P<0·0001). This was associated with an increased mean number of RBCs phagocytosed per THP-1 cell (5·2±0·6 and 3·3±0·2 respectively; P<0·002). Erythrophagocytosis of fractionated young and old RBCs increased with longer storage duration up to 28 days (P<0·05). However, no significant differences were observed between erythrophagocytosis of young and old RBCs. CONCLUSION:The susceptibility of stored RBCs to erythrophagocytosis significantly increased with longer storage time of the RBC units. Storage duration of RBCs had a greater influence on in vitro erythrophagocytosis than the chronological age of the RBCs at donation.

journal_name

Vox Sang

journal_title

Vox sanguinis

authors

Veale MF,Healey G,Sparrow RL

doi

10.1111/vox.12095

subject

Has Abstract

pub_date

2014-04-01 00:00:00

pages

219-26

issue

3

eissn

0042-9007

issn

1423-0410

journal_volume

106

pub_type

杂志文章
  • Cytomegalovirus (CMV) antibody in male homosexuals: a source for CMV immune globulin.

    abstract::Male homosexuals have been found to have an extraordinarily high prevalence, and high titers, of antibodies to cytomegalovirus (CMV), as determined by complement-fixation and passive hemagglutination tests. These findings support other data suggesting that CMV may be sexually transmitted. The high titers of CMV antibo...

    journal_title:Vox sanguinis

    pub_type: 杂志文章

    doi:10.1111/j.1423-0410.1983.tb01881.x

    authors: Ikram H,Prince AM,Baker LN

    更新日期:1983-03-01 00:00:00

  • White blood cell fragments in platelet concentrates prepared by the platelet-rich plasma or buffy-coat methods.

    abstract:BACKGROUND AND OBJECTIVES:White blood cell (WBC) fragments in platelet concentrates (PCs) may induce allo-immunization in the recipient. MATERIALS AND METHODS:As the level of WBC fragments can differ between PCs produced using different methods, we compared PCs prepared by using the buffy-coat method (BC-PCs) in plasm...

    journal_title:Vox sanguinis

    pub_type: 杂志文章

    doi:10.1111/j.1423-0410.2005.00634.x

    authors: Dijkstra-Tiekstra MJ,van der Schoot CE,Pietersz RN,Reesink HW

    更新日期:2005-05-01 00:00:00

  • A new method for phenotyping red blood cells using microplates.

    abstract:BACKGROUND AND OBJECTIVES:The supply of phenotyped red blood cells (RBC) for patients with several RBC antibodies presents a difficult task to hospital blood banks and regional blood centers. The aim of this study was to establish a low-cost typing system to allow extensive phenotyping of regular blood donors for clini...

    journal_title:Vox sanguinis

    pub_type: 杂志文章

    doi:10.1159/000031093

    authors: Llopis F,Carbonell-Uberos F,Montero MC,Bonanad S,Planelles MD,Plasencia I,Riol C,Planells T,Carrillo C,De Miguel A

    更新日期:1999-01-01 00:00:00

  • Clinical tolerance of methylene blue virus-inactivated plasma. A randomized crossover trial in 12 healthy human volunteers.

    abstract:OBJECTIVES:Baxter's methylene blue (MB) photoinactivation of fresh frozen plasma (FFP) is a method that effectively removes any intracellular potentially infectious agent, such as prions, by filtration combined with an improved photochemical virus inactivation on a single unit of FFP. The purpose of this study was to d...

    journal_title:Vox sanguinis

    pub_type: 临床试验,杂志文章,随机对照试验

    doi:10.1159/000031129

    authors: Simonsen AC,Sørensen H

    更新日期:1999-01-01 00:00:00

  • Value of central analysis of leucocyte depletion quality control data within the National Blood Service, England.

    abstract:BACKGROUND AND OBJECTIVES:The results of quality monitoring leucocyte counts were analysed nationally for the first 2 years of universal leucocyte depletion (LD), spanning the time-period before and after standardization of the counting and LD methods. The objectives were twofold: first to determine whether the impleme...

    journal_title:Vox sanguinis

    pub_type: 杂志文章

    doi:10.1046/j.1423-0410.2002.00210.x

    authors: Beckman N,Cardigan R,Wallington T,Williamson LM

    更新日期:2002-08-01 00:00:00

  • An autoantibody with activity dependent on red cell age in the serum of a patient with autoimmune haemolytic anaemia and a negative direct antiglobulin test.

    abstract::A red cell IgM autoantibody with anti-e specificity was identified in the serum of a rhesus-negative (rr) patient presenting with haemolytic anaemia and a negative direct antiglobulin test. The autoantibody strongly agglutinated normal allogeneic rhesus-negative (rr) red cells in saline at 37 degrees C but had only we...

    journal_title:Vox sanguinis

    pub_type: 杂志文章

    doi:10.1111/j.1423-0410.1987.tb02993.x

    authors: Herron R,Clark M,Smith DS

    更新日期:1987-01-01 00:00:00

  • Effect of periodate oxidation on specific activities and carbohydrate components of human blood group N- and M-specific glycoproteins and glycopeptides.

    abstract::Mild as well as strong periodate oxidation of isolated erythrocyte N and M glycoproteins and glycopeptides gave extensive to complete destruction of N-specificities as measured with Vicia graminea extracts and of N- as well as M- activities determined with all but 1 of 8 animal anti-N and 13 anti-M sera. Results with ...

    journal_title:Vox sanguinis

    pub_type: 杂志文章

    doi:10.1111/j.1423-0410.1978.tb02931.x

    authors: Springer GF,Yang HJ

    更新日期:1978-01-01 00:00:00

  • Plasma temperature during methylene blue/light treatment influences virus inactivation capacity and product quality.

    abstract:BACKGROUND:Photodynamic treatment using methylene blue (MB) and visible light is in routine use for pathogen inactivation of human plasma in different countries. Ambient and product temperature conditions for human plasma during production may vary between production sites. The influence of different temperature condit...

    journal_title:Vox sanguinis

    pub_type: 杂志文章

    doi:10.1111/vox.12643

    authors: Gravemann U,Handke W,Sumian C,Alvarez I,Reichenberg S,Müller TH,Seltsam A

    更新日期:2018-05-01 00:00:00

  • Transmission of parasites by blood transfusion.

    abstract::A number of parasitic diseases are known, or suspected to be transmitted by blood transfusion. Of greatest concern are malaria and Chagas' disease, but babesia, leishmania and toxoplasmosis also offer risk in particular locations or circumstances. Some of these parasites may be imported into non-endemic areas as a res...

    journal_title:Vox sanguinis

    pub_type: 杂志文章,评审

    doi:10.1111/j.1423-0410.1998.tb05415.x

    authors: Dodd RY

    更新日期:1998-01-01 00:00:00

  • Intrapatient comparison of an intermittent and a continuous flow cell separator for the collection of progenitor and stem cells from the blood.

    abstract:BACKGROUND AND OBJECTIVES:Continuous-flow and intermittent-flow blood cell separators (CFCS and IFCS) are both used to collect stem cells from the blood to rescue patients undergoing myeloablative treatment for cancer. MATERIALS AND METHODS:We designed a study to compare the collection efficiency of the two systems. T...

    journal_title:Vox sanguinis

    pub_type: 杂志文章

    doi:

    authors: Beretta F,van den Bosch S,Castelli D,Cavalli F,Ghielmini M

    更新日期:1998-01-01 00:00:00

  • Removal of hepatitis B virus infectivity from human gamma-globulin prepared by ion-exchange chromatography.

    abstract::Hepatitis B virus infectivity can be removed from human plasma used to prepare a gamma-globulin product by ion-exchange chromatography with commercially available resins. A pooled human plasma sample free of hepatitis markers was contaminated with 10,000 chimpanzee infectious doses of hepatitis B virus per milliliter ...

    journal_title:Vox sanguinis

    pub_type: 杂志文章

    doi:10.1111/j.1423-0410.1985.tb01130.x

    authors: Zolton RP,Padvelskis JV,Kaplan PM

    更新日期:1985-01-01 00:00:00

  • Blood donors infected with the hepatitis B virus but persistently lacking antibodies to the hepatitis B core antigen.

    abstract:BACKGROUND AND OBJECTIVES:Antibodies to the core of hepatitis B virus (anti-HBc) are considered to be the best serologically reliable markers of hepatitis B virus (HBV) infection. Through a national epidemiological survey, two young and first-time blood donors, originating from HBV-endemic areas, were identified as HBV...

    journal_title:Vox sanguinis

    pub_type: 杂志文章

    doi:10.1046/j.1423-0410.2001.00016.x

    authors: Laperche S,Guitton C,Smilovici W,Courouce AM

    更新日期:2001-02-01 00:00:00

  • Collaborative study to calibrate hepatitis C virus genotypes 2-6 against the HCV International Standard, 96/790 (genotype 1).

    abstract:BACKGROUND AND OBJECTIVES:A major requirement of a hepatitis C virus (HCV) RNA nucleic acid amplification technology (NAT) assay validation is the ability of the assay to detect the six major genotypes of HCV with equivalent sensitivities. The aim of this study was to characterize and calibrate an HCV genotype panel fo...

    journal_title:Vox sanguinis

    pub_type: 杂志文章

    doi:10.1046/j.1423-0410.2003.00260.x

    authors: Saldanha J,Heath A,Collaborative Study Group.

    更新日期:2003-01-01 00:00:00

  • Immunochemical characterization of the Rh CW antigen using human monoclonal antibodies.

    abstract:BACKGROUND AND OBJECTIVES:Human monoclonal antibodies have been produced against various Rh antigens. We report here the production of another one against Rh C and the first such antibody against Rh CW. MATERIALS AND METHODS:Two heterohybridomas secreting IgG human monoclonal antibodies against Rh CW and Rh C (MS-353 ...

    journal_title:Vox sanguinis

    pub_type: 杂志文章

    doi:10.1046/j.1423-0410.1997.7330174.x

    authors: Thorpe SJ,Boult CE,Thompson KM

    更新日期:1997-01-01 00:00:00

  • Systematic use of leukocyte-free blood components to prevent alloimmunization and platelet refractoriness in multitransfused children with cancer.

    abstract::A major drawback in the use of platelet transfusions is the development of platelet refractoriness that is usually caused by alloimmunization provoked by leukocytes present in the blood products. We evaluated the development of alloimmunization both by serological demonstration of HLA antibodies and by clinical demons...

    journal_title:Vox sanguinis

    pub_type: 临床试验,杂志文章

    doi:10.1111/j.1423-0410.1993.tb02168.x

    authors: Saarinen UM,Koskimies S,Myllylä G

    更新日期:1993-01-01 00:00:00

  • Epitope specificity and inactivation mechanisms of factor VIII inhibitor antibodies.

    abstract::The domain specificity of anti-factor VIII (FVIII) inhibitor antibodies was determined in assays using FVIII domains generated by thrombin cleavage or expressed as recombinant polypeptides to neutralise the inhibitor. The results revealed the existence of three major types of inhibitors, and various combinations of th...

    journal_title:Vox sanguinis

    pub_type: 杂志文章,评审

    doi:10.1159/000056708

    authors: Scandella D

    更新日期:1999-01-01 00:00:00

  • Cold lymphocytotoxins. Chromatographical study.

    abstract::A chromatographical study was made of eight antisera containing either anti-HL-A antibodies, cold lymphocytotoxins, or a mixture of both types of antibodies. The results vary according to the sera obtained. Usually cold lymphocytotoxins are sited in the IgM peak and anti-HL-A antibodies in the IgG peak, but there are ...

    journal_title:Vox sanguinis

    pub_type: 杂志文章

    doi:10.1111/j.1423-0410.1976.tb02207.x

    authors: Mayer S,Hauptmann G,Tongio MM

    更新日期:1976-01-01 00:00:00

  • Effect of beta-propiolactone on blood group antibody detection.

    abstract::A panel of 50 blood group antibodies covering a range of blood group antigens has been tested in the presence of 0.25% beta-propiolactone as a possible means of reducing infectivity of high-risk HTLV III/HBsAg samples. 11/50 (22%) antibodies could not be detected by the indirect antiglobulin test, and 6/40 (15%) were ...

    journal_title:Vox sanguinis

    pub_type: 杂志文章

    doi:10.1111/j.1423-0410.1986.tb00207.x

    authors: Hunt AF,Jones MT,Howkins GJ

    更新日期:1986-01-01 00:00:00

  • Detection of early anti-p24 HIV responses in EIA- and immunoblot-negative individuals. Implications for confirmatory testing.

    abstract::A sensitive and specific radioimmunoprecipitation assay was developed for the detection and analysis of anti-HIV antibody response in human sera with the use of 125I-labelled purified HIV proteins with subsequent sodium-dodecylsulfate gel electrophoresis (125I-RIPA). The 125I-RIPA was shown to be as specific but at le...

    journal_title:Vox sanguinis

    pub_type: 杂志文章

    doi:10.1111/j.1423-0410.1987.tb04910.x

    authors: Huisman JG,Winkel IN,Lelie PN,Tersmette M,Goudsmit J,Miedema F

    更新日期:1987-01-01 00:00:00

  • Management of fetal alloimmune thrombocytopenia by weekly in utero platelet transfusions.

    abstract::Alloimmune neonatal thrombocytopenia (ANT) may cause intracranial haemorrhage in utero as well as at delivery. Recent management has concentrated on attempts to minimise fetal thrombocytopenia and prevent its complications. This report describes further experience with the use of repeated intravascular transfusions of...

    journal_title:Vox sanguinis

    pub_type: 杂志文章

    doi:10.1111/j.1423-0410.1990.tb02054.x

    authors: Murphy MF,Pullon HW,Metcalfe P,Chapman JF,Jenkins E,Waters AH,Nicolaides KH,Mibashan RS

    更新日期:1990-01-01 00:00:00

  • Unusual serological response to hepatitis E virus in plasma donors consistent with re-infection.

    abstract::Hepatitis E virus (HEV)-positive plasma donations, identified by a plasma mini-pool screening approach, were analysed using serological methods for the presence of anti-HEV IgM and IgG. Avidity testing was performed on the IgG-reactive donations. Anti-HEV IgG with high avidity was observed in two donors together with ...

    journal_title:Vox sanguinis

    pub_type: 杂志文章

    doi:10.1111/vox.12294

    authors: Baylis SA,Crossan C,Corman VM,Blümel J,Scobie L,Dalton HR

    更新日期:2015-11-01 00:00:00

  • Quantification by ELISA of erythrocyte-bound C3 fragments expressing C3d and/or C3c epitopes in patients with factor I deficiency and with autoimmune diseases.

    abstract::A sensitive ELISA assay for quantifying erythrocyte (E) bound C3 fragments was developed. The assay employs a double-antibody sandwich technique, using polyclonal anti-C3d or anti-C3c antibodies to quantify C3 fragments, expressing C3d and/or C3c epitopes in washed, detergent-solubilized E. The assay detected 50-120 m...

    journal_title:Vox sanguinis

    pub_type: 杂志文章

    doi:10.1111/j.1423-0410.1989.tb02039.x

    authors: Møller Rasmussen J,Jepsen HH,Teisner B,Holmskov-Nielsen U,Rasmussen GG,Svehag SE

    更新日期:1989-01-01 00:00:00

  • Transfusion transmitted hepatitis C and non-A, non-B, non-C.

    abstract::The decline in the incidence of TAH from 1985-1990 was considerable and based on a variety of interventions that led to increasingly intensive scrutiny of potential donors. The decline in incidence observed since 1990, and particularly since 1992, has been truly extraordinary and based principally on the introduction ...

    journal_title:Vox sanguinis

    pub_type: 杂志文章,评审

    doi:10.1111/j.1423-0410.1994.tb04539.x

    authors: Alter HJ

    更新日期:1994-01-01 00:00:00

  • Investigation of the thrombin-generating capacity, evaluated by thrombogram, and clot formation evaluated by thrombelastography of platelets stored in the blood bank for up to 7 days.

    abstract:BACKGROUND AND OBJECTIVES:Transfusion based on the Thrombelastograph (TEG) results reduces transfusion requirements in cardiac surgery and in liver transplantation. Taking the pivotal role of thrombin generation in the coagulation process into consideration, the clinical utility of the TEG may, in part, depend on its r...

    journal_title:Vox sanguinis

    pub_type: 杂志文章

    doi:10.1111/j.1423-0410.2007.01011.x

    authors: Johansson PI,Svendsen MS,Salado J,Bochsen L,Kristensen AT

    更新日期:2008-02-01 00:00:00

  • Prospective evaluation of immune haemolysis in liver transplantation.

    abstract:BACKGROUND:Immune haemolysis in liver transplant (LT) can occur due to autoantibodies and alloantibodies. The aim of this study was to evaluate the prevalence and risk factors for immune haemolysis in LT. METHODS:A total of 175 consecutive patients were included. Multiorgan recipients were excluded. Samples, from befo...

    journal_title:Vox sanguinis

    pub_type: 杂志文章

    doi:10.1111/vox.12865

    authors: Brunetta DM,de Barros Carlos LM,Da Silva VFP,de Oliveira Alves TM,Macedo ÊS,Coelho GR,Vasconcelos JBM,De Francesco Daher E,Garcia JHP

    更新日期:2020-01-01 00:00:00

  • Delayed hemolytic transfusion reaction associated with Rh antibody anti-f: first reported case.

    abstract::A delayed hemolytic transfusion reaction (DHTR) occurring in a 30-year-old Caucasian man is described. His blood groups were B, CDe/cDE (R1R2, f-) K-, S-s+, Fy (a + b-), and Jk (a + b +). Eight days after receiving two units of packed red blood cells, his urine was strongly positive for hemoglobin; serum-free hemoglob...

    journal_title:Vox sanguinis

    pub_type: 杂志文章

    doi:10.1111/j.1423-0410.1985.tb00808.x

    authors: O'Reilly RA,Lombard CM,Azzi RL

    更新日期:1985-01-01 00:00:00

  • Human T-cell lymphotropic virus: A simulation model to estimate residual risk with universal leucoreduction and testing strategies in Canada.

    abstract:BACKGROUND AND OBJECTIVES:In Canada, transfusion transmission risk of Human T-cell lymphotropic virus -I/II (HTLV) is addressed by universal leucoreduction and universal antibody testing. We aimed to estimate the risk with the current policy, if testing only first-time donors and if testing were stopped. MATERIALS AND...

    journal_title:Vox sanguinis

    pub_type: 杂志文章

    doi:10.1111/vox.12722

    authors: O'Brien SF,Yi QL,Goldman M,Grégoire Y,Delage G

    更新日期:2018-11-01 00:00:00

  • Microbiology, time course and clinical characteristics of infection in critically ill patients receiving packed red blood cell transfusion.

    abstract:BACKGROUND AND OBJECTIVES:Packed red blood cell transfusion has been associated with increased infection in a variety of critically ill patient populations. We evaluated the microbiology and time course of infection in transfused patients in the intensive care unit (ICU) as no data exist on these parameters. MATERIALS...

    journal_title:Vox sanguinis

    pub_type: 杂志文章

    doi:10.1111/j.1423-0410.2009.01134.x

    authors: Rachoin JS,Daher R,Schorr C,Milcarek B,Parrillo JE,Gerber DR

    更新日期:2009-11-01 00:00:00

  • Lack of linkage between hereditary angioedema and the A and B loci of the HLA system.

    abstract::A large 81 member, four-generation black family with hereditary angioedema is reported with regards to its clinical course and the association with the histocompatibility system. No mortality was seen related directly to the disease. Assuming that this trait is autosomal dominant it appeared to have no linkage with th...

    journal_title:Vox sanguinis

    pub_type: 杂志文章

    doi:10.1111/j.1423-0410.1978.tb02911.x

    authors: Blumenthal MN,Dalmasso AP,Roitman B,Kelly J,Noreen H,Emmy L,Mendell NR,Yunis EJ

    更新日期:1978-09-01 00:00:00

  • Riboflavin and amotosalen photochemical treatments of platelet concentrates reduce thrombus formation kinetics in vitro.

    abstract:BACKGROUND:Photochemical treatment (PCT) of platelet concentrates using photosensitizers and ultraviolet light illumination reduces the proliferation potential of pathogens by damaging biomolecules. MATERIALS AND METHODS:The impact of riboflavin (RF-PRT)- and amotosalen (AS-PCT)-based pathogen inactivation on platelet...

    journal_title:Vox sanguinis

    pub_type: 杂志文章

    doi:10.1111/vox.12231

    authors: Van Aelst B,Feys HB,Devloo R,Vanhoorelbeke K,Vandekerckhove P,Compernolle V

    更新日期:2015-05-01 00:00:00