Abstract:
BACKGROUND AND OBJECTIVES:Continuous-flow and intermittent-flow blood cell separators (CFCS and IFCS) are both used to collect stem cells from the blood to rescue patients undergoing myeloablative treatment for cancer. MATERIALS AND METHODS:We designed a study to compare the collection efficiency of the two systems. The continuous-flow Cobe Spectra and the intermittent-flow Haemonetics MCS-3P were used to collect cells on consecutive days from 9 patients mobilised with G-CSF with or without chemotherapy. Blood obtained before leukapheresis and the leukapheresis product were analysed for their content of red and white cells, platelets, CD34-positive cells, GM-CFC, CFC-E, and BFU-E. An extraction ratio was calculated. RESULTS:We found that the CFCS extracted about 4 times more mononuclear cells per unit time, 3 times more CD34-positive, and 4 times more clonogenic cells than the IFCS. The subject acceptability of the two systems was similar. CONCLUSION:The CFCS is a more efficient system for stem cell collection. IFCS requires a longer harvesting time for the same result.
journal_name
Vox Sangjournal_title
Vox sanguinisauthors
Beretta F,van den Bosch S,Castelli D,Cavalli F,Ghielmini Msubject
Has Abstractpub_date
1998-01-01 00:00:00pages
149-53issue
2eissn
0042-9007issn
1423-0410pii
vox75149journal_volume
75pub_type
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