Intrapatient comparison of an intermittent and a continuous flow cell separator for the collection of progenitor and stem cells from the blood.

Abstract:

BACKGROUND AND OBJECTIVES:Continuous-flow and intermittent-flow blood cell separators (CFCS and IFCS) are both used to collect stem cells from the blood to rescue patients undergoing myeloablative treatment for cancer. MATERIALS AND METHODS:We designed a study to compare the collection efficiency of the two systems. The continuous-flow Cobe Spectra and the intermittent-flow Haemonetics MCS-3P were used to collect cells on consecutive days from 9 patients mobilised with G-CSF with or without chemotherapy. Blood obtained before leukapheresis and the leukapheresis product were analysed for their content of red and white cells, platelets, CD34-positive cells, GM-CFC, CFC-E, and BFU-E. An extraction ratio was calculated. RESULTS:We found that the CFCS extracted about 4 times more mononuclear cells per unit time, 3 times more CD34-positive, and 4 times more clonogenic cells than the IFCS. The subject acceptability of the two systems was similar. CONCLUSION:The CFCS is a more efficient system for stem cell collection. IFCS requires a longer harvesting time for the same result.

journal_name

Vox Sang

journal_title

Vox sanguinis

authors

Beretta F,van den Bosch S,Castelli D,Cavalli F,Ghielmini M

subject

Has Abstract

pub_date

1998-01-01 00:00:00

pages

149-53

issue

2

eissn

0042-9007

issn

1423-0410

pii

vox75149

journal_volume

75

pub_type

杂志文章
  • Pst I polymorphism of the antithrombin III gene in a French population.

    abstract::Pst I endonuclease fragment length polymorphism of the antithrombin III probe was studied in a French population. Among 25 subjects tested, 15 were found heterozygous at the Pst I site, so that this polymorphism might serve as a useful markers of antithrombin III gene in families with antithrombin III deficiency. ...

    journal_title:Vox sanguinis

    pub_type: 杂志文章

    doi:10.1111/j.1423-0410.1985.tb00787.x

    authors: Le Paslier D,Rochu D,Lucotte G

    更新日期:1985-01-01 00:00:00

  • Syphilis serology in blood donors: a possible surrogate marker for human immunodeficiency virus risk.

    abstract::We report a preliminary study on whether syphilis serology might be reactive in some blood donors at risk for human immunodeficiency virus (HIV) infection. We retrospectively analyzed voluntary blood donations with reactive Treponema pallidum antibody (TPA) tests according to the type of donation, the presence of othe...

    journal_title:Vox sanguinis

    pub_type: 杂志文章

    doi:10.1111/j.1423-0410.1991.tb00895.x

    authors: Ramsey G,Soltis F,Bowman R,McNamee J,Hahn LF,Dixon BW

    更新日期:1991-01-01 00:00:00

  • A new method to collect granulocytes using a low dose of hydroxyethyl starch.

    abstract::A new granulocyte collection method employing discontinuous flow centrifugation and low donor dose of hydroxyethyl starch (HES) is presented. Following the procedure, the increase in serum polysaccharide concentration was 100 mg/dl, about 20% of that seen after standard discontinuous flow leukapheresis. The average ef...

    journal_title:Vox sanguinis

    pub_type: 杂志文章

    doi:10.1111/j.1423-0410.1983.tb04111.x

    authors: Szymanski IO

    更新日期:1983-01-01 00:00:00

  • An electronic method for counting human T and B cell rosettes.

    abstract::This study reports the adaptation of an electronic particle-counting method for the enumeration of T cell and B cell rosettes of human lymphocytes. This method and the conventional hemocytometer method were compared in parallel on seventeen T cell and seven B cell rosette enumerations in a blind study design. All data...

    journal_title:Vox sanguinis

    pub_type: 杂志文章

    doi:10.1111/j.1423-0410.1979.tb02298.x

    authors: Denny TN,Kinsley KM,Benson JW,Bull RW

    更新日期:1979-01-01 00:00:00

  • An improved method to study complement receptor-mediated function of the fixed macrophage system in vivo.

    abstract::A method to coat unsensitized erythrocytes with fragments of C3 and C4 using autologous serum, in order to study complement receptor-dependent function of the fixed macrophage system, is presented. After incubation with serum under optimal conditions, at least 90% of the cells had C3b/iC3b deposited on the surface, wi...

    journal_title:Vox sanguinis

    pub_type: 杂志文章

    doi:10.1111/j.1423-0410.1991.tb00926.x

    authors: Nilsson Ekdahl K,Lööf L,Nilsson UR,Nilsson B

    更新日期:1991-01-01 00:00:00

  • What would encourage blood donation in Ireland?

    abstract:BACKGROUND:Recent changes have resulted in the loss of 4% of the donor panel in the Republic of Ireland and 3% in Northern Ireland. In order to increase the number of donors in these two regions, it is important that transfusion service providers explore and understand the reasons, which prevent individuals from donati...

    journal_title:Vox sanguinis

    pub_type: 杂志文章

    doi:10.1111/j.1423-0410.2007.00893.x

    authors: Harrington M,Sweeney MR,Bailie K,Morris K,Kennedy A,Boilson A,O'Riordan J,Staines A

    更新日期:2007-05-01 00:00:00

  • World Health Organization collaborative study to calibrate the 3rd International Standard for Hepatitis C virus RNA nucleic acid amplification technology (NAT)-based assays.

    abstract:BACKGROUND AND OBJECTIVES:A collaborative study was undertaken to evaluate a replacement World Health Organization International Standard for hepatitis C virus (HCV) RNA for nucleic acid amplification technology (NAT)-based assays. The candidate preparations were calibrated in International Units (IUs). MATERIALS AND ...

    journal_title:Vox sanguinis

    pub_type: 杂志文章,多中心研究

    doi:10.1111/j.1423-0410.2010.01434.x

    authors: Baylis SA,Heath AB,Collaborative Study Group.

    更新日期:2011-05-01 00:00:00

  • Thrombocytopenia at birth in neonates with red cell alloimmune haemolytic disease.

    abstract:OBJECTIVE:To evaluate the incidence and severity of and risk factors for thrombocytopenia at birth in neonates with red cell alloimmunization. STUDY DESIGN:All neonates with haemolytic disease of the foetus/newborn (HDFN) due to red cell alloimmunization admitted to our centre between January 2000 and September 2010 w...

    journal_title:Vox sanguinis

    pub_type: 杂志文章,随机对照试验

    doi:10.1111/j.1423-0410.2011.01539.x

    authors: Rath ME,Smits-Wintjens VE,Oepkes D,van Zwet EW,van Kamp IL,Brand A,Walther FJ,Lopriore E

    更新日期:2012-04-01 00:00:00

  • Iron deficiency among French whole-blood donors: first assessment and identification of predictive factors.

    abstract:BACKGROUND:The objectives of this study are to estimate the prevalence of iron deficiency (ID) among French whole-blood (WB) donors to identify factors associated with ID and to generate decision trees. STUDY DESIGN AND METHODS:A prospective National multicentre study was performed on WB donors from March 11, to April...

    journal_title:Vox sanguinis

    pub_type: 杂志文章

    doi:10.1111/vox.12991

    authors: Fillet AM,Martinaud C,Malard L,Le Cam S,Hejl C,Chenus F,Woimant G,Chueca M,Jacquot E,Besiers C,Morel P,Djoudi R,Garrabé E,Gross S

    更新日期:2021-01-01 00:00:00

  • The effect of previous blood transfusion on lymphocyte subsets and natural killer cell function in patients with colorectal cancer.

    abstract::To elucidate the possible influence of previous blood transfusion on immune functions, the transfusion history of 153 patients admitted to hospital for elective colorectal surgery was correlated with lymphocyte subsets and natural killer (NK) cell function. The subsets determined were CD2, CD3, CD4, CD8, CD16, CD20, C...

    journal_title:Vox sanguinis

    pub_type: 杂志文章

    doi:10.1111/j.1423-0410.1994.tb05035.x

    authors: Mathiesen O,Lund L,Brodthagen U,Gandrup P,Grunnet N,Balslev I,Jersild C

    更新日期:1994-01-01 00:00:00

  • Screening blood donors for Plasmodium falciparum malariae. Application of ready-to-use homologous antigens.

    abstract::Prevention of post-transfusion malaria remains a worrying problem. Until now it was very difficult to obtain homologous antigens, and immunologic methods used to detect malaria were expensive. We tested the ready-to-use Plasmodium falciparum prepared slides using the IFA (indirect fluorescent antibody) test on 866 sel...

    journal_title:Vox sanguinis

    pub_type: 杂志文章

    doi:10.1111/j.1423-0410.1983.tb01933.x

    authors: Deroff P,Reguer M,Simitzis AM,Boudon A,Saleun JP

    更新日期:1983-01-01 00:00:00

  • Accumulation of soluble inflammatory mediators between blood donation and pre-storage leucocyte depletion.

    abstract:BACKGROUND AND OBJECTIVES:Leucocyte-derived cytokines accumulate in stored whole blood. Pre-storage leucocyte depletion has reduced cytokine levels and, consequently, febrile non-haemolytic transfusion reactions. As leucocyte filtration and component separation can be performed until 24 h after donation, we hypothesize...

    journal_title:Vox sanguinis

    pub_type: 杂志文章

    doi:10.1111/j.1423-0410.2008.01132.x

    authors: Chudziak D,Sireis W,Pfeiffer HU,Henschler R,Seifried E,Bönig H

    更新日期:2009-02-01 00:00:00

  • Longer storage of red blood cells is associated with increased in vitro erythrophagocytosis.

    abstract:BACKGROUND AND OBJECTIVES:Refrigerated storage of red blood cells (RBCs) induces numerous changes that may target the cells for erythrophagocytosis following transfusion. The influence of storage upon the phagocytosis of unseparated and fractionated young and old stored RBCs was investigated using two in vitro quantita...

    journal_title:Vox sanguinis

    pub_type: 杂志文章

    doi:10.1111/vox.12095

    authors: Veale MF,Healey G,Sparrow RL

    更新日期:2014-04-01 00:00:00

  • Biochemical and biological properties of an alpha 1-antitrypsin concentrate.

    abstract::alpha 1-Antitrypsin (AAT) has been purified from human plasma supernatant A (equivalent to COHN fraction II + III) by a large-scale chromatographic procedure involving anion-exchange adsorption on DEAE Sepharose CL-6B fast flow and size-exclusion chromatography on Sephacryl S-200. Before freeze-drying, the liquid conc...

    journal_title:Vox sanguinis

    pub_type: 杂志文章

    doi:10.1111/j.1423-0410.1987.tb04895.x

    authors: Burnouf T,Constans J,Clerc A,Descamps J,Martinache L,Goudemand M

    更新日期:1987-01-01 00:00:00

  • Hemovigilance in the United States of America.

    abstract::Although hemovigilance is not a word used commonly in the United States, the concept of monitoring morbidity associated with blood transfusion is accepted widely. Currently, quality management focuses on error avoidance rather than error detection in both the "manufacturing" and "clinical" aspects of blood banking. "M...

    journal_title:Vox sanguinis

    pub_type: 杂志文章,评审

    doi:10.1111/j.1423-0410.1998.tb05455.x

    authors: Menitove JE

    更新日期:1998-01-01 00:00:00

  • A new red blood cell cold autoantibody (anti-Me)

    abstract::A new red blood cell (RBC) cold autoantibody (anti-Me) is presented which was detected in a patient with autoimmune hemolytic anemia of 'cold type' associated with Waldenström's macroglobulinemia. The autoantibody was of the IgM (Kappa) class, had a moderate titer, and reacted with cord as well as with adult RBC. Its ...

    journal_title:Vox sanguinis

    pub_type: 杂志文章

    doi:10.1111/j.1423-0410.1985.tb01122.x

    authors: Salama A,Pralle H,Mueller-Eckhardt C

    更新日期:1985-01-01 00:00:00

  • Oxygenating the microcirculation: the perspective from blood transfusion and blood storage.

    abstract::Tissue oxygen delivery depends on red blood cell (RBC) content and RBC flow regulation in the microcirculation. The important role of the RBC in tissue oxygenation is clear from anaemia and the use of RBC transfusion which has saved many lives. Whether RBC transfusion actually restores tissue oxygenation is difficult ...

    journal_title:Vox sanguinis

    pub_type: 杂志文章,评审

    doi:10.1111/j.1423-0410.2007.00909.x

    authors: Raat NJ,Ince C

    更新日期:2007-07-01 00:00:00

  • Therapeutic efficacy of platelet components treated with amotosalen and ultraviolet A pathogen inactivation method: results of a meta-analysis of randomized controlled trials.

    abstract:BACKGROUND AND OBJECTIVES:There are conflicting data regarding the therapeutic efficacy of platelets inactivated using amotosalen and ultraviolet A light. We have performed a meta-analysis to summarize the results of different randomized controlled trials (RCT). MATERIALS AND METHODS:Five RCTs reported through March 2...

    journal_title:Vox sanguinis

    pub_type: 杂志文章,meta分析

    doi:10.1111/j.1423-0410.2012.01614.x

    authors: Cid J,Escolar G,Lozano M

    更新日期:2012-11-01 00:00:00

  • Safety of leucodepleted salvaged blood in oncological surgery: an in vitro model.

    abstract::Intra-operative blood cell salvage (IOCS) is mainly avoided in onco surgery due to the suspicion that it could increase metastasis' risk. We simulated IOCS followed by leucodepletion: HCT116 (human colorectal cancer) cells were inoculated into packed red blood cells units, and their distribution was evaluated, step-by...

    journal_title:Vox sanguinis

    pub_type: 杂志文章

    doi:10.1111/vox.12565

    authors: Marraccini C,Merolle L,Berni P,Boito K,Tamagnini I,Kuhn E,Ragazzi M,Baricchi R,Pertinhez TA

    更新日期:2017-11-01 00:00:00

  • Use of monoclonal anti-C3 antibodies to characterise the fragments of C3 that are found on erythrocytes.

    abstract::Using monoclonal antibodies to C3 it has been shown that the red blood cells of patients with cold haemagglutinin disease carry on their cells C3d,g (alpha-2D-globulin) rather than C3d. C3d,g seems to be the final product of in vivo C3 activation in fluid phase and on red cells. The cleavage of C3dg to C3d and C3g doe...

    journal_title:Vox sanguinis

    pub_type: 杂志文章

    doi:10.1111/j.1423-0410.1983.tb01928.x

    authors: Lachmann PJ,Voak D,Oldroyd RG,Downie DM,Bevan PC

    更新日期:1983-01-01 00:00:00

  • A method for estimating the residual risk of transfusion-transmitted HBV infection associated with occult hepatitis B virus infection in a donor population without universal anti-HBc screening.

    abstract:BACKGROUND AND OBJECTIVES:This report describes a method for estimating the risk of transfusion-transmitted HBV infection attributable to blood components from donors with occult hepatitis B virus infection (OBI) applicable where universal anti-HBc screening is not performed. MATERIALS AND METHODS:In the context of pa...

    journal_title:Vox sanguinis

    pub_type: 杂志文章

    doi:10.1111/vox.12060

    authors: Seed CR,Kiely P

    更新日期:2013-11-01 00:00:00

  • An autoantibody with activity dependent on red cell age in the serum of a patient with autoimmune haemolytic anaemia and a negative direct antiglobulin test.

    abstract::A red cell IgM autoantibody with anti-e specificity was identified in the serum of a rhesus-negative (rr) patient presenting with haemolytic anaemia and a negative direct antiglobulin test. The autoantibody strongly agglutinated normal allogeneic rhesus-negative (rr) red cells in saline at 37 degrees C but had only we...

    journal_title:Vox sanguinis

    pub_type: 杂志文章

    doi:10.1111/j.1423-0410.1987.tb02993.x

    authors: Herron R,Clark M,Smith DS

    更新日期:1987-01-01 00:00:00

  • The effect of plasmapheresis on blood pressure in voluntary plasma donors.

    abstract:BACKGROUND AND OBJECTIVES:Donor plasmapheresis involves the removal of a weight-adjusted volume of plasma and the return of cellular components to the donor. Although plasma volume generally returns to normal, some residual effect on vital signs may be possible. This analysis was performed to determine the possible eff...

    journal_title:Vox sanguinis

    pub_type: 杂志文章

    doi:10.1111/vox.12188

    authors: Rosa-Bray M,Wisdom C,Marier JF,Mouksassi MS,Wada S

    更新日期:2015-01-01 00:00:00

  • Anti-A production by a group O spleen transplanted to a group A recipient.

    abstract::A group A1 diabetic received a pancreas-spleen transplant from a group 0 donor. Severe immune hemolysis due to anti-A ensued, requiring graft splenectomy. The transplanted spleen can be a potent source of blood group antibody. ...

    journal_title:Vox sanguinis

    pub_type: 杂志文章

    doi:10.1111/j.1423-0410.1985.tb00187.x

    authors: Salamon DJ,Ramsey G,Nusbacher J,Yang S,Starzl TE,Israel L

    更新日期:1985-01-01 00:00:00

  • Occurrence and characterization of lympho-agglutinins in Indian plants.

    abstract::A survey of the occurrence of lympho-agglutinins was carried out on seeds of 150 species of wild and cultivated plants. Potent agglutinins were detected in 17 extracts by employing lymphocytes of man, guinea pig, goat and sheep in the bioassay. Except for one species each of Mimosoideae, Euphorbiaceae and Clusiaceae, ...

    journal_title:Vox sanguinis

    pub_type: 杂志文章

    doi:10.1111/j.1423-0410.1982.tb00034.x

    authors: Sandhu RS,Kamboj SS,Reen RS,Arora JS

    更新日期:1982-01-01 00:00:00

  • Selective depression of blood group antigens associated with hereditary ovalocytosis among melanesians.

    abstract::Recessively inherited ovalocytosis in coastal Melanesians is associated with widespread, but selective, depression of blood group antigens in homozygotes, who comprise about 15% of these populations. It is suggested that a membrane anomaly exists, and that the series of depressed determinants all depend, for their ful...

    journal_title:Vox sanguinis

    pub_type: 杂志文章

    doi:10.1111/j.1423-0410.1977.tb00612.x

    authors: Booth PB,Serjeantson S,Woodfield DG,Amato D

    更新日期:1977-01-01 00:00:00

  • Clinical consequences of the extremely rare anti-PP1Pk isoantibodies in pregnancy: a case series and review of the literature.

    abstract:BACKGROUND:The absence of the red cell antigens P, P1 and Pk , known as 'p', represents an extremely rare red cell phenotype. Individuals with this phenotype spontaneously form anti-PP1Pk isoantibodies, associated with severe haemolytic transfusion reactions, recurrent spontaneous abortion and haemolytic disease of the...

    journal_title:Vox sanguinis

    pub_type: 杂志文章

    doi:10.1111/vox.13042

    authors: Di Ciaccio P,Cutts B,Alahakoon TI,Dennington PM,Soo LA,Curnow J

    更新日期:2020-12-16 00:00:00

  • Radioimmunoassay of hepatitis B core antigen and antibody with autologous reagents.

    abstract::Anti-HBc is the most sensitive indicator of current or recent hepatitis B virus infection, hence its detection is valuable in laboratory diagnosis of the infection. Dane particles isolated from the plasma of a single chronic carrier and his autologous IgG labeled with 125I were utilized in a solid-phase sandwich radio...

    journal_title:Vox sanguinis

    pub_type: 杂志文章

    doi:10.1111/j.1423-0410.1977.tb02259.x

    authors: Vyas GN,Roberts IM

    更新日期:1977-12-01 00:00:00

  • Transfusion practice in massively bleeding patients: time for a change?

    abstract:BACKGROUND AND OBJECTIVES:We identified some fatal cases where massively bleeding patients received inadequate transfusion therapy. The aim of this study was to review and evaluate the transfusion practice in acutely multitransfused patients. MATERIALS AND METHODS:Patients receiving > 10 units of red blood cells (RBC)...

    journal_title:Vox sanguinis

    pub_type: 杂志文章

    doi:10.1111/j.1423-0410.2005.00668.x

    authors: Johansson PI,Hansen MB,Sørensen H

    更新日期:2005-08-01 00:00:00

  • Report of the Second International Workshop on molecular blood group genotyping.

    abstract::The second International Society of Blood Transfusion and International Council for Standardization in Haematology workshop on molecular blood group genotyping was held in 2006. Forty-one laboratories participated. Six samples were distributed: two representing DNA from transfusion-dependent patients for testing for a...

    journal_title:Vox sanguinis

    pub_type:

    doi:10.1111/j.1423-0410.2007.00926.x

    authors: Daniels G,van der Schoot CE,Olsson ML

    更新日期:2007-07-01 00:00:00