An electronic method for counting human T and B cell rosettes.

abstract：:Since 2000, Quality Assurance (QA) exercises for the detection and identification of granulocyte antibodies and DNA typing for human neutrophil antigens (HNA) have been distributed within the International Granulocyte Immunobiology Workshops, which are linked to International Society of Blood Transfusion. The exercise...

journal_title：Vox sanguinis

authors： Lucas G,Porcelijn L,Fung YL,Green F,Reil A,Hopkins M,Schuller R,Green A,de Haas M,Bux J

更新日期：2013-10-01 00:00:00

abstract：:We have employed a PCR approach to determine the nucleotide sequences of the coding region in the last two coding exons of the histo-blood group ABO genes from one A(X) and one B(A) individual. Compared with A1 alleles, the (A(X)) allele has a single nucleotide substitution (T-->A at nucleotide 646) resulting in an am...

journal_title：Vox sanguinis

authors： Yamamoto F,McNeill PD,Yamamoto M,Hakomori S,Harris T

更新日期：1993-01-01 00:00:00

abstract：:The human Lewis gene encodes an alpha(1,3/1,4)-fucosyltransferase responsible for synthesis of the Le(a) and a Le(b) antigens. To define the molecular background for non-functional Lewis genes we have sequenced PCR-amplified DNA fragments from two Le(a-b-) individuals. One was homozygously mutated at nucleotides 202(T...

journal_title：Vox sanguinis

authors： Elmgren A,Börjeson C,Svensson L,Rydberg L,Larson G

更新日期：1996-01-01 00:00:00

abstract：:Platelet concentrates were prepared in plastic packs of polyvinyl chloride with tri(2-ethylhexyl) trimellitate as plasticizer. They were stored, with gentle shaking, at room temperature for periods up to 7 days before labelling with isotope and reinfusing. In vivo survival studies, platelet counts, pH and electron mic...

journal_title：Vox sanguinis

authors： Archer GT,Grimsley PG,Jindra J,Robson JE,Ribeiro A

更新日期：1982-01-01 00:00:00

abstract：:Anti-Vw detecting an antigen on Miltenberger I (Mi I) variant glycophorin A (GPA) has rarely been reported as a cause of hemolytic disease of the newborn (HDN). We report an infant with severe HDN due to anti-Vw. Examination of the Vw+ erythrocytes of the father and paternal grandmother by sodium dodecylsulphate polya...

journal_title：Vox sanguinis

authors： Rearden A,Frandson S,Carry JB

更新日期：1987-01-01 00:00:00

abstract：BACKGROUND AND OBJECTIVES:Transfusion based on the Thrombelastograph (TEG) results reduces transfusion requirements in cardiac surgery and in liver transplantation. Taking the pivotal role of thrombin generation in the coagulation process into consideration, the clinical utility of the TEG may, in part, depend on its r...

journal_title：Vox sanguinis

authors： Johansson PI,Svendsen MS,Salado J,Bochsen L,Kristensen AT

更新日期：2008-02-01 00:00:00

abstract：BACKGROUND AND OBJECTIVES:Our post-thaw cell recovery rates differed substantially in interlaboratory comparisons of identical samples, potentially due to different temperatures during cell staining. MATERIALS AND METHODS:Viable CD34(+) cells and leucocyte (WBC) subtypes were quantified by multiparameter single-platfo...

journal_title：Vox sanguinis

authors： Fritsch G,Frank N,Dmytrus J,Frech C,Pichler H,Witt V,Geyeregger R,Scharner D,Trbojevic D,Zipperer E,Printz D,Worel N

更新日期：2016-08-01 00:00:00

abstract：OBJECTIVES:Positive direct antiglobulin tests (DAT) occur occasionally, without obvious cause, among normal blood donors. This is sometimes associated with a false-positive serologic test for syphilis. Positive DATs due to antiphospholipid antibodies (APAbs) are well documented in the antiphospholipid syndrome with sys...

journal_title：Vox sanguinis

authors： Win N,Islam SI,Peterkin MA,Walker ID

更新日期：1997-01-01 00:00:00

abstract：:Forty-six subjects (44 HIV antibody-positive) with some degree of immune deficiency (at least TH/TS ratio below 1) were randomly distributed into 4 treatment groups. Each group was assigned to 1 of 4 products to be used exclusively for a 1-year period: 1 concentrate was of intermediate purity and not heat-treated, and...

journal_title：Vox sanguinis

authors： Allain JP,Frommel D,Bosser C,Gazengel C,Larrieu MJ,Sultan Y

更新日期：1987-01-01 00:00:00

abstract：BACKGROUND AND OBJECTIVES:Cold-stored platelets may be an alternative to conventional room temperature (RT) storage. However, cold-stored platelets are cleared more rapidly from circulation, reducing their suitability for prophylactic transfusion. To minimise wastage, it may be beneficial to store platelets conventiona...

journal_title：Vox sanguinis

authors： Wood B,Johnson L,Hyland RA,Marks DC

更新日期：2018-04-06 00:00:00

abstract：:Ocular chronic GVHD is efficaciously treated with autologous platelet-derived eye drops. We investigated the cytokine content of eye drops produced using a non-gelified lysate obtained from autologous platelet-rich plasma in six patients with ocular GVHD. In both the responding (n = 4) and the resistant (n = 2) patien...

journal_title：Vox sanguinis

authors： Valentini CG,Nuzzolo ER,Orlando N,Metafuni E,Bianchi M,Chiusolo P,Zini G,Teofili L

更新日期：2016-02-01 00:00:00

abstract：:The Am and Bm phenotypes are characterized by weak expression of the A or B antigens, respectively, by red blood cells with a normal expression by the saliva of secretors. Deletion of the regulatory element in the first intron of the ABO gene and disruption of the GATA motif in the element were found to be responsible...

journal_title：Vox sanguinis

authors： Oda A,Isa K,Ogasawara K,Kameyama K,Okuda K,Hirashima M,Ishii H,Kimura K,Matsukura H,Hirayama F,Kawa K

更新日期：2015-05-01 00:00:00

abstract：:Following transfusion, red cells labeled with nonradioactive chromium (52Cr) can be detected in the circulation using atomic absorption spectrophotometry. Both in rhesus monkeys and in human subjects, the survival of red cells labeled with 52Cr was found to be insignificantly different from that of cells labeled with ...

journal_title：Vox sanguinis

authors： Sioufi HA,Button LN,Jacobson MS,Kevy SV

更新日期：1990-01-01 00:00:00

abstract：BACKGROUND AND OBJECTIVES:Previous data that showed maintenance of reticulocyte percentage in whole blood stored in CPDA-1 have led to the assumption that reticulocyte maturation becomes arrested during refrigerated storage. However, reticulocyte behaviour in red-blood-cell units stored in additive solutions has not ye...

journal_title：Vox sanguinis

authors： Urbina A,Palomino F

更新日期：2013-05-01 00:00:00

abstract：BACKGROUND AND OBJECTIVES:The results of quality monitoring leucocyte counts were analysed nationally for the first 2 years of universal leucocyte depletion (LD), spanning the time-period before and after standardization of the counting and LD methods. The objectives were twofold: first to determine whether the impleme...

journal_title：Vox sanguinis

authors： Beckman N,Cardigan R,Wallington T,Williamson LM

更新日期：2002-08-01 00:00:00

abstract：OBJECTIVES:To determine whether the attitudes, beliefs and motivations of blood donors evolve over time. MATERIALS AND METHODS:Using questionnaires a 7-year longitudinal study of a cohort of 126 donors was performed to gather their sociodemographic characteristics and their attitudes, beliefs and motivations relating ...

journal_title：Vox sanguinis

authors： Fernández-Montoya A,López-Berrio A,Luna del Castillo JD

更新日期：1998-01-01 00:00:00

abstract：:A simple method, based on ethanol fractionation, for the preparation of highly purified human serum albumin with a higher yield than that of the conventional ethanol procedures is described. It consists of two purification steps, namely, precipitation of most of the other plasma proteins from a 3-fold diluted plasma w...

journal_title：Vox sanguinis

authors： Hao Y

更新日期：1979-01-01 00:00:00

abstract：BACKGROUND AND OBJECTIVES:In peripheral blood stem cell transplantation, the number of CD34(+) cells infused is considered a predictor of haematopoietic engraftment. However, the currently accepted minimal threshold of CD34(+) cells/kg was determined by counting CD34(+) cells before freezing, and the loss of viable CD3...

journal_title：Vox sanguinis

authors： Lee S,Kim S,Kim H,Baek EJ,Jin H,Kim J,Kim HO

更新日期：2008-02-01 00:00:00

abstract：:Since its discovery, human plasminogen (PLG) polymorphism has received widespread acceptance in population genetics and forensic haematology. Due to the large number of variant alleles described, a PLG reference typing and Plasminogen Symposium was held, at which a nomenclature proposal was inaugurated. The technology...

journal_title：Vox sanguinis

authors： Skoda U,Bertrams J,Dykes D,Eiberg H,Hobart M,Hummel K,Kühnl P,Mauff G,Nakamura S,Nishimukai H

更新日期：1986-01-01 00:00:00

abstract：:A combined immunoassay for the simultaneous detection of antibodies to HIV-1/2 and HTLV-I/II (Bioelisa, Launch Diagnostics) has been evaluated to determine its suitability for routine use in blood screening. 84,222 donations were tested from 76,452 donors. One HIV- and 1 HTLV-1-positive donor were identified. The spec...

journal_title：Vox sanguinis

authors： Flanagan P,McAlpine L,Ramskill SJ,Smith AG,Eglin R,Parry JV,Mortimer PP

更新日期：1995-01-01 00:00:00

abstract：BACKGROUND AND OBJECTIVES:Packed red blood cell transfusion has been associated with increased infection in a variety of critically ill patient populations. We evaluated the microbiology and time course of infection in transfused patients in the intensive care unit (ICU) as no data exist on these parameters. MATERIALS...

journal_title：Vox sanguinis

authors： Rachoin JS,Daher R,Schorr C,Milcarek B,Parrillo JE,Gerber DR

更新日期：2009-11-01 00:00:00

abstract：:The successful cloning of a non-structural antigen from the genome of what is now designated as the 'hepatitis C virus' (HCV) has transformed an erstwhile diagnosis of exclusion for non-A, non-B hepatitis (NANBH). The assay has been validated against panels of known infectivity for NANBH and sera from haemophiliac pat...

journal_title：Vox sanguinis

authors： Barbara JA,Contreras M

更新日期：1991-01-01 00:00:00

abstract：BACKGROUND AND OBJECTIVES:A major requirement of a hepatitis C virus (HCV) RNA nucleic acid amplification technology (NAT) assay validation is the ability of the assay to detect the six major genotypes of HCV with equivalent sensitivities. The aim of this study was to characterize and calibrate an HCV genotype panel fo...

journal_title：Vox sanguinis

authors： Saldanha J,Heath A,Collaborative Study Group.

更新日期：2003-01-01 00:00:00

abstract：BACKGROUND AND OBJECTIVES:White blood cell (WBC) fragments in platelet concentrates (PCs) may induce allo-immunization in the recipient. MATERIALS AND METHODS:As the level of WBC fragments can differ between PCs produced using different methods, we compared PCs prepared by using the buffy-coat method (BC-PCs) in plasm...

journal_title：Vox sanguinis

authors： Dijkstra-Tiekstra MJ,van der Schoot CE,Pietersz RN,Reesink HW

更新日期：2005-05-01 00:00:00

abstract：:After more than 20 years the extended pedigree of the only known kindred carrying the low frequency antigen Ria (Ridley) was reinvestigated. It is established that Ria is governed by a gene which is part of or very closely linked to the MNSs locus. Further serological and frequency studies are reported. ...

journal_title：Vox sanguinis

authors： Contreras M,Armitage SE,Stebbing B

更新日期：1984-01-01 00:00:00

abstract：BACKGROUND AND OBJECTIVES:The last two decades have seen major developments in genotyping assays to facilitate the procurement of red blood cell units to alloimmunized patients. To make genotyping faster, simpler and less costly, a nanotechnology approach based on metal/silica fluorescent nanoparticles and a polymer-ba...

journal_title：Vox sanguinis

authors： Brouard D,Ratelle O,Perreault J,Boudreau D,St-Louis M

更新日期：2015-02-01 00:00:00

abstract：:The authors have studied the incidence of hepatitis associated antigen (HAA) and the homologous antibody in sera of patients with rheumatoid arthritis on the basis of (1) arthritis sometimes associated with viral hepatitis, (2) the possible infectious etiology of rheumatoid arthritis, and (3) observation on the possib...

journal_title：Vox sanguinis

authors： Marcolongo R,Debolini A

更新日期：1975-01-01 00:00:00

abstract：BACKGROUND AND OBJECTIVES:Neonatal alloimmune thrombocytopenia is usually attributable to HPA-la antibodies. We report a case of parental platelet antigen incompatibility associated with a severe neonatal thrombocytopenia secondary to alloimmunization to HPA-3a. MATERIALS AND METHODS:Platelet antibodies were detected ...

journal_title：Vox sanguinis

authors： Boehlen F,Kaplan C,de Moerloose P

更新日期：1998-01-01 00:00:00

abstract：BACKGROUND AND OBJECTIVES:Recombinant activated factor VII (rFVIIa, NovoSeven) has been used off-label for various conditions. A protocol for its use in acute, uncontrolled life-threatening bleeding, was devised and employed. A haematologist/transfusion specialist was assigned as a member of the team. MATERIALS AND ME...

journal_title：Vox sanguinis

authors： Mayo A,Misgav M,Kluger Y,Geenberg R,Pauzner D,Klausner J,Ben-Tal O

更新日期：2004-07-01 00:00:00

abstract：BACKGROUND:The low-prevalence Rh antigen, JAL, was named after the index case, Mr. J. Allen. Based on reactivity of seven multi-specific sera with his RBCs, it was apparent that they express at least one additional low-prevalence antigen. The purpose of this study was to investigate the other low-prevalence antigen(s) ...

journal_title：Vox sanguinis

authors： Hue-Roye K,Reid ME,Westhoff CM,Lomas-Francis C

更新日期：2011-07-01 00:00:00