A simple method for the preparation of human serum albumin.

Abstract:

:A simple method, based on ethanol fractionation, for the preparation of highly purified human serum albumin with a higher yield than that of the conventional ethanol procedures is described. It consists of two purification steps, namely, precipitation of most of the other plasma proteins from a 3-fold diluted plasma with ethanol at 42% concentration, pH 5.75 and -5 degrees C, leaving over 96% pure albumin in the supernatant, followed by isoelectric precipitation of albumin from the supernatant at pH 4.8 and -5 degrees C. The paste thus obtained was processed to the final albumin solution according to the conventional methods. The yield of the final albumin with a purity of over 99% was equivalent to 29.5 g/l of plasma representing a recovery of over 93%. The possibility of recovering other plasma proteins and the suitability for large scale preparation are also discussed.

journal_name

Vox Sang

journal_title

Vox sanguinis

authors

Hao Y

doi

10.1111/j.1423-0410.1979.tb04441.x

subject

Has Abstract

pub_date

1979-01-01 00:00:00

pages

313-20

issue

5

eissn

0042-9007

issn

1423-0410

journal_volume

36

pub_type

杂志文章
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    doi:10.1111/j.1423-0410.1985.tb00189.x

    authors: Dinning G,Doughty RW,Collins AK

    更新日期:1985-01-01 00:00:00

  • Determinants of transfusion decisions in the ICU: haemoglobin concentration, what else? - a retrospective cohort study.

    abstract:BACKGROUND AND OBJECTIVES:The aim of this study was to assess potentially relevant clinical characteristics which influence the decision to transfuse red cells in critically ill patients with low haemoglobin concentrations (6.0-10.0 g/dl). MATERIALS AND METHODS:This was a retrospective observational cohort study of pa...

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    journal_title:Vox sanguinis

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    doi:10.1111/j.1423-0410.1994.tb04541.x

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    更新日期:1994-01-01 00:00:00

  • Delayed hemolytic transfusion reaction associated with Rh antibody anti-f: first reported case.

    abstract::A delayed hemolytic transfusion reaction (DHTR) occurring in a 30-year-old Caucasian man is described. His blood groups were B, CDe/cDE (R1R2, f-) K-, S-s+, Fy (a + b-), and Jk (a + b +). Eight days after receiving two units of packed red blood cells, his urine was strongly positive for hemoglobin; serum-free hemoglob...

    journal_title:Vox sanguinis

    pub_type: 杂志文章

    doi:10.1111/j.1423-0410.1985.tb00808.x

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  • Prospective study of posttransfusion hepatitis in cardiac surgery patients receiving only blood or also blood products.

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    doi:10.1111/j.1423-0410.1983.tb04099.x

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  • Persistence of Parvovirus B19 (B19V) DNA and humoral immune response in B19V-infected blood donors.

    abstract:BACKGROUND AND OBJECTIVES:Parvovirus B19 (B19V) DNA seems to persist in the plasma of B19V-infected blood donors. The relevance of this for recipients of single-donor blood components is yet unclear. MATERIAL AND METHODS:We studied serial archive and follow-up samples from 75 B19V-infected blood donors to obtain more ...

    journal_title:Vox sanguinis

    pub_type: 杂志文章

    doi:10.1111/vox.12162

    authors: Juhl D,Görg S,Hennig H

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  • The influence of platelet glycoprotein polymorphisms on receptor function and risk for thrombosis.

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    pub_type: 杂志文章,评审

    doi:10.1111/j.1423-0410.2002.tb05274.x

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  • Current concepts for quality assured long-distance transport of temperature-sensitive red blood cell concentrates.

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    journal_title:Vox sanguinis

    pub_type: 杂志文章

    doi:10.1111/j.1423-0410.2009.01302.x

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    更新日期:2010-07-01 00:00:00

  • Transmission of parasites and bacteria by blood components.

    abstract:BACKGROUND AND OBJECTIVES:Although most attention has been paid to viral infections as a complication of transfusion, some parasitic infections are readily transmissible and generate a heavy burden of disease, particularly in the developing world. Additionally, bacterial infection as a result of transfusion is the most...

    journal_title:Vox sanguinis

    pub_type: 杂志文章,评审

    doi:

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    更新日期:2000-01-01 00:00:00

  • Population study of heterophile antibodies.

    abstract::Sera from 2,829 participants in a population health survey were tested for the presence of a heterophilic antibody, detectable by immunofluorescence. The antibody was found in 9.8% of the sera from women and in 12.4% of the sera from men. The age-specific prevalence of the antibody was relatively constant in women but...

    journal_title:Vox sanguinis

    pub_type: 杂志文章

    doi:10.1111/j.1423-0410.1980.tb02973.x

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    更新日期:1980-12-01 00:00:00

  • Blood transfusion in preterm infants improves intestinal tissue oxygenation without alteration in blood flow.

    abstract:BACKGROUND AND OBJECTIVE:The objective of the study was to investigate the splanchnic blood flow velocity and oximetry response to blood transfusion in preterm infants according to postnatal age. MATERIALS AND METHODS:Preterm infants receiving blood transfusion were recruited to three groups: 1-7 (group 1; n = 20), 8-...

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    pub_type: 杂志文章

    doi:10.1111/vox.12436

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  • The Sdx antigen and antibody: biochemical studies on the inhibitory property of human urine.

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    journal_title:Vox sanguinis

    pub_type: 杂志文章

    doi:10.1111/j.1423-0410.1983.tb01884.x

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    更新日期:1983-03-01 00:00:00

  • A1 weak B phenotypes found in two generations of a Romanian family: A1Bx versus cis AB complex.

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    journal_title:Vox sanguinis

    pub_type: 杂志文章

    doi:10.1111/j.1423-0410.1975.tb00593.x

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    更新日期:1975-01-01 00:00:00

  • Rapid thawing of fresh-frozen plasma with radio wave-based thawing technology and effects on coagulation factors during prolonged storage at 4 degrees C.

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    journal_title:Vox sanguinis

    pub_type: 杂志文章

    doi:10.1111/j.1423-0410.2009.01175.x

    authors: Boström F,Ekemar L,Olsson D,Egberg N,Lundahl J

    更新日期:2009-07-01 00:00:00

  • The serology of Sda effects of transfusion and pregnancy.

    abstract::Pre- and posttransfusion antibody titers were performed on 6 patients with anti-Sda transfused with incompatible blood. In 3 of these patients a significant rise in IgG antibody titer was found. The data suggest that in occasional patients the Sda antigen does evoke a secondary immune response. We evaluated 245 pregna...

    journal_title:Vox sanguinis

    pub_type: 杂志文章

    doi:10.1111/j.1423-0410.1982.tb01104.x

    authors: Spitalnik S,Cox MT,Spennacchio J,Guenther R,Blumberg N

    更新日期:1982-01-01 00:00:00

  • Transfusion transmitted hepatitis C and non-A, non-B, non-C.

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    journal_title:Vox sanguinis

    pub_type: 杂志文章,评审

    doi:10.1111/j.1423-0410.1994.tb04539.x

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    更新日期:1994-01-01 00:00:00

  • "VA", a new type of erythrocyte polyagglutination characterized by depressed H receptors and associated with hemolytic anemia. I. Serological and hematological observations.

    abstract::This report describes a case of persistent polyagglutinability restriced to the red blood cells, and associated with hemolytic anemia in a 20-year-old male. AIME WITH NORMAL INTERVALS. His red blood cells were weakly agglutinated by almost all adult sera. No autoagglutination was noted and the direct antihuman globuli...

    journal_title:Vox sanguinis

    pub_type: 杂志文章

    doi:

    authors: Graninger W,Rameis H,Fischer K,Poschmann A,Bird GW,Wingham J,Neumann E

    更新日期:1977-01-01 00:00:00

  • Automated serological compatibility testing using a solid-phase test and standard laboratory equipment.

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    journal_title:Vox sanguinis

    pub_type: 杂志文章

    doi:10.1046/j.1423-0410.2001.00034.x

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    更新日期:2001-05-01 00:00:00

  • The strategies to reduce iron deficiency in blood donors randomized trial: design, enrolment and early retention.

    abstract:BACKGROUND AND OBJECTIVES:Repeated blood donation produces iron deficiency. Changes in dietary iron intake do not prevent donation-induced iron deficiency. Prolonging the interdonation interval or using oral iron supplements can mitigate donation-induced iron deficiency. The most effective operational methods for reduc...

    journal_title:Vox sanguinis

    pub_type: 临床试验,杂志文章,随机对照试验

    doi:10.1111/vox.12210

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    更新日期:2015-02-01 00:00:00

  • Sensitivity of assays for the detection of HPA-1a antibodies: results of an international workshop demonstrating the impact of cation chelation from integrin αIIbβ3 on three widely used assays.

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    journal_title:Vox sanguinis

    pub_type: 杂志文章

    doi:10.1111/vox.12043

    authors: Allen DL,Metcalfe P,Kaplan C,Kekomaki R,de Haas M,Yusuf R,Ouwehand WH

    更新日期:2013-08-01 00:00:00

  • Purification of human immunoglobulin G: a new approach to plasma fractionation.

    abstract:BACKGROUND AND OBJECTIVES:Currently, plasma fractionation involves multiple processing steps using established methods such as ethanol precipitation and column chromatography. The known limitations associated with conventional purification techniques, combined with strict regulations on safety and high demand for parti...

    journal_title:Vox sanguinis

    pub_type: 杂志文章

    doi:10.1046/j.1423-0410.2002.00241.x

    authors: Li G,Stewart R,Conlan B,Gilbert A,Roeth P,Nair H

    更新日期:2002-11-01 00:00:00

  • A multicentre evaluation of a new filtration protocol for leucocyte depletion of high-haematocrit red blood cells collected by an automated blood collection system.

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    journal_title:Vox sanguinis

    pub_type: 临床试验,杂志文章,多中心研究

    doi:10.1046/j.1423-0410.2001.00078.x

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    更新日期:2001-01-01 00:00:00

  • Modified formulation of CPDA for storage of whole blood, and of SAGM for storage of red blood cells, to maintain the concentration of 2,3-diphosphoglycerate.

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    journal_title:Vox sanguinis

    pub_type: 杂志文章

    doi:10.1111/j.0042-9007.2003.00366.x

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    更新日期:2003-11-01 00:00:00

  • Effect of periodate oxidation on specific activities and carbohydrate components of human blood group N- and M-specific glycoproteins and glycopeptides.

    abstract::Mild as well as strong periodate oxidation of isolated erythrocyte N and M glycoproteins and glycopeptides gave extensive to complete destruction of N-specificities as measured with Vicia graminea extracts and of N- as well as M- activities determined with all but 1 of 8 animal anti-N and 13 anti-M sera. Results with ...

    journal_title:Vox sanguinis

    pub_type: 杂志文章

    doi:10.1111/j.1423-0410.1978.tb02931.x

    authors: Springer GF,Yang HJ

    更新日期:1978-01-01 00:00:00

  • Weak D alleles in Japanese: a c.960G>A silent mutation in exon 7 of the RHD gene that affects D expression.

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    pub_type: 杂志文章

    doi:10.1111/vox.12322

    authors: Ogasawara K,Sasaki K,Isa K,Tsuneyama H,Uchikawa M,Satake M,Tadokoro K

    更新日期:2016-02-01 00:00:00

  • Not every PRP-gel is born equal. Evaluation of growth factor availability for tissues through four PRP-gel preparations: Fibrinet, RegenPRP-Kit, Plateltex and one manual procedure.

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    journal_title:Vox sanguinis

    pub_type: 杂志文章

    doi:10.1111/j.1423-0410.2009.01188.x

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    更新日期:2009-08-01 00:00:00

  • Human alloantibodies detecting a red cell antigen apparently identical to MER2.

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    pub_type: 杂志文章

    doi:10.1111/j.1423-0410.1988.tb05085.x

    authors: Daniels GL,Levene C,Berrebi A,Schechter Y,Moulds M,Sela R,Poole J,Lacey P,Atkins CJ

    更新日期:1988-01-01 00:00:00

  • Differences in substrate specificities for cysteine proteinases used in blood group serology, and the use of bromelain in a two-phase inhibitor technique.

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    journal_title:Vox sanguinis

    pub_type: 杂志文章

    doi:10.1111/j.1423-0410.1989.tb04988.x

    authors: Ogasawara K,Mazda T

    更新日期:1989-01-01 00:00:00

  • Acute hypoproteinemic fluid overload: its determinants, distribution, and treatment with concentrated albumin and diuretics.

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    pub_type: 杂志文章

    doi:10.1111/j.1423-0410.1977.tb04481.x

    authors: Pappova E,Bachmeier W,Crevoisier JL,Kollar J,Kollar M,Tobler P,Zahler HW,Zaugg D,Lundsgaard-Hansen P

    更新日期:1977-01-01 00:00:00

  • A comparative study of platelets stored in polyvinyl chloride containers plasticised with butyryl trihexyl citrate or triethylhexyl trimellitate.

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    pub_type: 杂志文章

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    更新日期:1995-01-01 00:00:00