Abstract:
:A simple method, based on ethanol fractionation, for the preparation of highly purified human serum albumin with a higher yield than that of the conventional ethanol procedures is described. It consists of two purification steps, namely, precipitation of most of the other plasma proteins from a 3-fold diluted plasma with ethanol at 42% concentration, pH 5.75 and -5 degrees C, leaving over 96% pure albumin in the supernatant, followed by isoelectric precipitation of albumin from the supernatant at pH 4.8 and -5 degrees C. The paste thus obtained was processed to the final albumin solution according to the conventional methods. The yield of the final albumin with a purity of over 99% was equivalent to 29.5 g/l of plasma representing a recovery of over 93%. The possibility of recovering other plasma proteins and the suitability for large scale preparation are also discussed.
journal_name
Vox Sangjournal_title
Vox sanguinisauthors
Hao Ydoi
10.1111/j.1423-0410.1979.tb04441.xsubject
Has Abstractpub_date
1979-01-01 00:00:00pages
313-20issue
5eissn
0042-9007issn
1423-0410journal_volume
36pub_type
杂志文章相关文献
VOX SANGUINIS文献大全abstract::An example of a cold-reacting IgM anti-K2 is described. The patient has a history of blood transfusion and two pregnancies. It is, therefore, not possible to differentiate between natural occurrence or immune stimulation as an explanation of this antibody's presence. In 1966, Thomas and Konugres reported an unusual ex...
journal_title:Vox sanguinis
pub_type: 杂志文章
doi:10.1111/j.1423-0410.1985.tb00189.x
更新日期:1985-01-01 00:00:00
abstract:BACKGROUND AND OBJECTIVES:The aim of this study was to assess potentially relevant clinical characteristics which influence the decision to transfuse red cells in critically ill patients with low haemoglobin concentrations (6.0-10.0 g/dl). MATERIALS AND METHODS:This was a retrospective observational cohort study of pa...
journal_title:Vox sanguinis
pub_type: 杂志文章
doi:10.1111/vox.12831
更新日期:2019-11-01 00:00:00
abstract::Marrow transplantation is increasing, hematopoietic stem cells are being obtained from a wider variety of donors, and the cells almost always undergo some processing steps. These activities, along with the hematologic complexities of marrow transplant patients, create many varied opportunities for blood centers. ...
journal_title:Vox sanguinis
pub_type: 杂志文章,评审
doi:10.1111/j.1423-0410.1994.tb04541.x
更新日期:1994-01-01 00:00:00
abstract::A delayed hemolytic transfusion reaction (DHTR) occurring in a 30-year-old Caucasian man is described. His blood groups were B, CDe/cDE (R1R2, f-) K-, S-s+, Fy (a + b-), and Jk (a + b +). Eight days after receiving two units of packed red blood cells, his urine was strongly positive for hemoglobin; serum-free hemoglob...
journal_title:Vox sanguinis
pub_type: 杂志文章
doi:10.1111/j.1423-0410.1985.tb00808.x
更新日期:1985-01-01 00:00:00
abstract::The incidence, etiology and risk factors of posttransfusion (PT) hepatitis were evaluated in a prospective study of 297 consecutive open-heart surgery patients. PT hepatitis occurred in 63 (21.2%) patients with a significantly higher hepatitis attack rate in 51 recipients of commercial clotting factor concentrates (56...
journal_title:Vox sanguinis
pub_type: 杂志文章
doi:10.1111/j.1423-0410.1983.tb04099.x
更新日期:1983-01-01 00:00:00
abstract:BACKGROUND AND OBJECTIVES:Parvovirus B19 (B19V) DNA seems to persist in the plasma of B19V-infected blood donors. The relevance of this for recipients of single-donor blood components is yet unclear. MATERIAL AND METHODS:We studied serial archive and follow-up samples from 75 B19V-infected blood donors to obtain more ...
journal_title:Vox sanguinis
pub_type: 杂志文章
doi:10.1111/vox.12162
更新日期:2014-10-01 00:00:00
abstract::With regard to hemostasis and thrombosis, collagens are among the most important physiologic components of the extracellular matrix in their role as platelet activators. Differences in the rate of platelet activation markedly influence normal hemostasis and the pathological outcome of thrombosis. Thus, collagen recept...
journal_title:Vox sanguinis
pub_type: 杂志文章,评审
doi:10.1111/j.1423-0410.2002.tb05274.x
更新日期:2002-08-01 00:00:00
abstract:BACKGROUND AND OBJECTIVES:The German Armed Forces Blood Service in Koblenz supplies red blood cell concentrates (RBCs) to military and civilian institutions at home and to field hospitals during peacekeeping operations abroad. During long-distance transport, blood products can be exposed to extreme environmental condit...
journal_title:Vox sanguinis
pub_type: 杂志文章
doi:10.1111/j.1423-0410.2009.01302.x
更新日期:2010-07-01 00:00:00
abstract:BACKGROUND AND OBJECTIVES:Although most attention has been paid to viral infections as a complication of transfusion, some parasitic infections are readily transmissible and generate a heavy burden of disease, particularly in the developing world. Additionally, bacterial infection as a result of transfusion is the most...
journal_title:Vox sanguinis
pub_type: 杂志文章,评审
doi:
更新日期:2000-01-01 00:00:00
abstract::Sera from 2,829 participants in a population health survey were tested for the presence of a heterophilic antibody, detectable by immunofluorescence. The antibody was found in 9.8% of the sera from women and in 12.4% of the sera from men. The age-specific prevalence of the antibody was relatively constant in women but...
journal_title:Vox sanguinis
pub_type: 杂志文章
doi:10.1111/j.1423-0410.1980.tb02973.x
更新日期:1980-12-01 00:00:00
abstract:BACKGROUND AND OBJECTIVE:The objective of the study was to investigate the splanchnic blood flow velocity and oximetry response to blood transfusion in preterm infants according to postnatal age. MATERIALS AND METHODS:Preterm infants receiving blood transfusion were recruited to three groups: 1-7 (group 1; n = 20), 8-...
journal_title:Vox sanguinis
pub_type: 杂志文章
doi:10.1111/vox.12436
更新日期:2016-11-01 00:00:00
abstract::Anti-Sdx is an IgM complement-binding autoantibody that defines a red cell antigen which is independent of I, i, Sp1 (Pr) and Gd. Hemagglutination by the antibody is unusually sensitive to variation in pH, salt, or other charged molecular species. The antibody is inhibited by urine from Sd(a+) persons, but inhibition ...
journal_title:Vox sanguinis
pub_type: 杂志文章
doi:10.1111/j.1423-0410.1983.tb01884.x
更新日期:1983-03-01 00:00:00
abstract::Three 'A1 weak B' blood groups found in two generations of a Romanian family were studied with quantitative and thermodynamic assays in order to determine if they derive from 'A1Bx' or from 'cis AB' genotypes, what cannot be achieved by the genetics. ...
journal_title:Vox sanguinis
pub_type: 杂志文章
doi:10.1111/j.1423-0410.1975.tb00593.x
更新日期:1975-01-01 00:00:00
abstract:BACKGROUND AND OBJECTIVES:With traditional techniques the thawing time for fresh-frozen plasma (FFP) is about 20-30 min. A new technology using radio waves, radio wave thawing device (RTD), was applied for thawing FFP. With this technology the thawing time can substantially be reduced. The new technique was compared to...
journal_title:Vox sanguinis
pub_type: 杂志文章
doi:10.1111/j.1423-0410.2009.01175.x
更新日期:2009-07-01 00:00:00
abstract::Pre- and posttransfusion antibody titers were performed on 6 patients with anti-Sda transfused with incompatible blood. In 3 of these patients a significant rise in IgG antibody titer was found. The data suggest that in occasional patients the Sda antigen does evoke a secondary immune response. We evaluated 245 pregna...
journal_title:Vox sanguinis
pub_type: 杂志文章
doi:10.1111/j.1423-0410.1982.tb01104.x
更新日期:1982-01-01 00:00:00
abstract::The decline in the incidence of TAH from 1985-1990 was considerable and based on a variety of interventions that led to increasingly intensive scrutiny of potential donors. The decline in incidence observed since 1990, and particularly since 1992, has been truly extraordinary and based principally on the introduction ...
journal_title:Vox sanguinis
pub_type: 杂志文章,评审
doi:10.1111/j.1423-0410.1994.tb04539.x
更新日期:1994-01-01 00:00:00
abstract::This report describes a case of persistent polyagglutinability restriced to the red blood cells, and associated with hemolytic anemia in a 20-year-old male. AIME WITH NORMAL INTERVALS. His red blood cells were weakly agglutinated by almost all adult sera. No autoagglutination was noted and the direct antihuman globuli...
journal_title:Vox sanguinis
pub_type: 杂志文章
doi:
更新日期:1977-01-01 00:00:00
abstract:BACKGROUND AND OBJECTIVES:To prove the feasibility of a semi-automated cross-match procedure using a commercially available solid-phase microplate test and standard laboratory equipment. MATERIAL AND METHODS:The new procedure was evaluated against the conventional spin tube technique and the gel centrifugation system....
journal_title:Vox sanguinis
pub_type: 杂志文章
doi:10.1046/j.1423-0410.2001.00034.x
更新日期:2001-05-01 00:00:00
abstract:BACKGROUND AND OBJECTIVES:Repeated blood donation produces iron deficiency. Changes in dietary iron intake do not prevent donation-induced iron deficiency. Prolonging the interdonation interval or using oral iron supplements can mitigate donation-induced iron deficiency. The most effective operational methods for reduc...
journal_title:Vox sanguinis
pub_type: 临床试验,杂志文章,随机对照试验
doi:10.1111/vox.12210
更新日期:2015-02-01 00:00:00
abstract:BACKGROUND AND OBJECTIVES:HPA-1a antibodies account for 70-80% of cases of fetal-neonatal alloimmune thrombocytopenia (FNAIT) in Caucasians. However, numerous workshops have demonstrated variability in their detection. We recently showed that exposure of αIIbβ3 to ethylene diamine tetraacetic acid (EDTA) affected bindi...
journal_title:Vox sanguinis
pub_type: 杂志文章
doi:10.1111/vox.12043
更新日期:2013-08-01 00:00:00
abstract:BACKGROUND AND OBJECTIVES:Currently, plasma fractionation involves multiple processing steps using established methods such as ethanol precipitation and column chromatography. The known limitations associated with conventional purification techniques, combined with strict regulations on safety and high demand for parti...
journal_title:Vox sanguinis
pub_type: 杂志文章
doi:10.1046/j.1423-0410.2002.00241.x
更新日期:2002-11-01 00:00:00
abstract:BACKGROUND AND OBJECTIVES:A multicentre trial was set up to evaluate the performance of a new leucodepletion protocol. MATERIALS AND METHODS:Filtration at high haematocrit was started during collection of red blood cell (RBC) products by apheresis with Trima. SAG-M was added after filtration through the filter. Haemat...
journal_title:Vox sanguinis
pub_type: 临床试验,杂志文章,多中心研究
doi:10.1046/j.1423-0410.2001.00078.x
更新日期:2001-01-01 00:00:00
abstract:BACKGROUND AND OBJECTIVES:A dramatic decrease in the level of 2,3-diphosphoglycerate (2,3-DPG) takes place during the storage of whole blood (WB) in CPDA (citrate-phosphate-dextrose-adenine) and a similar decrease occurs during the storage of red blood cells (RBCs) in SAGM (saline-adenine-glucose-mannitol). The aim of ...
journal_title:Vox sanguinis
pub_type: 杂志文章
doi:10.1111/j.0042-9007.2003.00366.x
更新日期:2003-11-01 00:00:00
abstract::Mild as well as strong periodate oxidation of isolated erythrocyte N and M glycoproteins and glycopeptides gave extensive to complete destruction of N-specificities as measured with Vicia graminea extracts and of N- as well as M- activities determined with all but 1 of 8 animal anti-N and 13 anti-M sera. Results with ...
journal_title:Vox sanguinis
pub_type: 杂志文章
doi:10.1111/j.1423-0410.1978.tb02931.x
更新日期:1978-01-01 00:00:00
abstract:BACKGROUND AND OBJECTIVES:The molecular basis of the weak D phenotype has been investigated for many years, and more than 80 different alleles producing weak D phenotypes have been identified. Most alleles producing weak D phenotypes have a single missense mutation in exons corresponding to a transmembrane domain of th...
journal_title:Vox sanguinis
pub_type: 杂志文章
doi:10.1111/vox.12322
更新日期:2016-02-01 00:00:00
abstract:BACKGROUND:The rationale for using topical platelet gel therapy is to provide the healing tissues with concentrated platelet-derived factors. Several systems are available to prepare platelet-rich plasma (PRP) and from these, the platelet gel. These systems produce two- to six-fold platelet and growth factor-enriched c...
journal_title:Vox sanguinis
pub_type: 杂志文章
doi:10.1111/j.1423-0410.2009.01188.x
更新日期:2009-08-01 00:00:00
abstract::Three examples of an antibody were found to be detecting a red cell polymorphism probably identical to MER2. The antibodies were made by Jews originating from India and living in Israel. Two of them were sibs and the third was unrelated. All 3 had kidney disease requiring renal dialysis and regular blood transfusion. ...
journal_title:Vox sanguinis
pub_type: 杂志文章
doi:10.1111/j.1423-0410.1988.tb05085.x
更新日期:1988-01-01 00:00:00
abstract::Because substrate specificities differ between proteolytic enzymes and because knowledge of the optimal enzyme activity levels is necessary in order to standardize procedures used in antibody screening, a study was made of the best common assay method for the routinely employed enzymes bromelain, papain and ficin. Cas...
journal_title:Vox sanguinis
pub_type: 杂志文章
doi:10.1111/j.1423-0410.1989.tb04988.x
更新日期:1989-01-01 00:00:00
abstract::We simulated the use of massive volumes of crystalloid fluids as a treatment of acute plasma loss in a standardized experimental model and studied the factors determining the retention or excretion of the resulting acute hypoproteinemic fluid overload, its distribution within the body, and its treatment with concentra...
journal_title:Vox sanguinis
pub_type: 杂志文章
doi:10.1111/j.1423-0410.1977.tb04481.x
更新日期:1977-01-01 00:00:00
abstract::Platelet concentrates (PCs), stored for 5 days in PL 2209, a new polyvinyl chloride (PVC) storage container plasticised with butyryl trihexyl citrate, were compared with those stored in PL 1240, a PVC platelet container plasticised with triethylhexyl trimellitate. In part 1 of the study, pooled platelet-rich plasma (P...
journal_title:Vox sanguinis
pub_type: 杂志文章
doi:10.1111/j.1423-0410.1995.tb02593.x
更新日期:1995-01-01 00:00:00