Differences in substrate specificities for cysteine proteinases used in blood group serology, and the use of bromelain in a two-phase inhibitor technique.

Abstract:

:Because substrate specificities differ between proteolytic enzymes and because knowledge of the optimal enzyme activity levels is necessary in order to standardize procedures used in antibody screening, a study was made of the best common assay method for the routinely employed enzymes bromelain, papain and ficin. Casein degradation was found better suited to this purpose than azoalbumin. With standardization achieved, a useful two-phase bromelain inhibitor technique was devised using bromelain at 20 casein units of activity. This method improved upon the one-stage bromelain technique in terms of sensitivity, freedom from false positive reactions and it compared well with the two-phase papain inhibitor technique.

journal_name

Vox Sang

journal_title

Vox sanguinis

authors

Ogasawara K,Mazda T

doi

10.1111/j.1423-0410.1989.tb04988.x

subject

Has Abstract

pub_date

1989-01-01 00:00:00

pages

72-6

issue

1

eissn

0042-9007

issn

1423-0410

journal_volume

57

pub_type

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