Immunochemical characterization of the Rh CW antigen using human monoclonal antibodies.

Abstract:

BACKGROUND AND OBJECTIVES:Human monoclonal antibodies have been produced against various Rh antigens. We report here the production of another one against Rh C and the first such antibody against Rh CW. MATERIALS AND METHODS:Two heterohybridomas secreting IgG human monoclonal antibodies against Rh CW and Rh C (MS-353 and MS-242 respectively) were produced from alloimmunized donors. Their specificity was confirmed by serological testing. RESULTS:MS-353 reacted with all CW-positive phenotypes but not with any CW-negative phenotypes, whereas MS-242 reacted with all CW-positive and C-positive phenotypes. Using 125I-labelled antibodies, the average number of CW sites/red cell was 32,000 (R1WR1W, 15,200 (R1WR1), 19,800 (R1WR2), 15,300 (R1Wr), 26,200 (r'Wr), and the average number of C sites per red cell on equivalent CW/C-positive phenotypes was similar: 22,400 (R1R1), 21,800 (R1WR1), 12,300 (R1R2), 11,700 (R1WR2), 13,400 (R1r), 15,100 (R1Wr), 21,100 (r'r), 18,700 (r'Wr). MS-353 and MS-242 were mutually inhibitory. Both antibodies specifically immunoprecipitated a 33- to 34-kD polypeptide from 125I-surface-labelled cells. CONCLUSION:MS-353 is suitable for the serological detection of CW-positive cells, and the immunochemical data are entirely consistent with the report that the CW antigen is associated with a point mutation in the RHCE gene [Blood, 1995;86:1196-1201].

journal_name

Vox Sang

journal_title

Vox sanguinis

authors

Thorpe SJ,Boult CE,Thompson KM

doi

10.1046/j.1423-0410.1997.7330174.x

subject

Has Abstract

pub_date

1997-01-01 00:00:00

pages

174-81

issue

3

eissn

0042-9007

issn

1423-0410

journal_volume

73

pub_type

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