A simplified method for the preparation of immune-serum globulin.

Abstract:

:This report describes the recovery of highly purified immune-serum globulin (ISG) from the 42% ethanol precipitate fraction (42% P) generated during the first step of albumin purification. The method consists of three purification steps: (1) reprecipitation of the 42% P at 20% ethanol, pH 7.2; (2) solubilization of ISG at 17% ethanol, pH 5.2, and (3) isoelectric precipitation of ISG at 25% ethanol, pH 7.2. ISG prepared by this method was homogeneous when subjected to immunoelectrophoresis and polyacrylamide gel electrophoresis, and gave a yield equivalent to 4.7 g/l plasma, corresponding to a final recovery of over 48%.

journal_name

Vox Sang

journal_title

Vox sanguinis

authors

Hao YL,Wickerhauser M

doi

10.1111/j.1423-0410.1981.tb00706.x

subject

Has Abstract

pub_date

1981-04-01 00:00:00

pages

278-85

issue

4

eissn

0042-9007

issn

1423-0410

journal_volume

40

pub_type

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