Molecular bases of the ABO blood groups of Indians from the Brazilian Amazon region.

Abstract:

:Phenotype studies of ABO blood groups in most Amerindian populations revealed the exclusive presence of group O. Since group O is the result of the absence of glycosyltransferase activity, its molecular bases may be heterogeneous. We carried out ABO blood group genotyping by analysis of DNA of 30 Indians from 2 Amazonian tribes (Yanomami and Arara), and compared the findings with other populations (Caucasians and Blacks). Two segments of the glycosyltransferase gene were amplified by PCR and digested with KpnI or AluI to detect deletion or base change at positions 258 and 700, respectively. For all subjects, the gene basis of blood group O is the deletion of a single nucleotide at position 258 of the glycosyltransferase A gene, similar to that observed in Caucasoids and Negroids. DNA sequencing of limited regions of the gene supports this conclusion. This finding does not exclude, however, that a heterogeneity of the O allele may be revealed by a more extensive analysis.

journal_name

Vox Sang

journal_title

Vox sanguinis

authors

Franco RF,Simões BP,Guerreiro JF,Santos SE,Zago MA

doi

10.1111/j.1423-0410.1994.tb01255.x

subject

Has Abstract

pub_date

1994-01-01 00:00:00

pages

299-301

issue

3

eissn

0042-9007

issn

1423-0410

journal_volume

67

pub_type

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