Biofilm-associated accumulation-associated protein (Aap): A contributing factor to the predominant growth of Staphylococcus epidermidis in platelet concentrates.


BACKGROUND AND OBJECTIVES:Staphylococcus epidermidis is a predominant contaminant of platelet concentrates (PCs), outcompeting other skin flora bacteria such as Staphylococcus capitis. The accumulation-associated protein (Aap), encoded by the aap gene, is involved in formation of bacterial aggregates (biofilms) in S. epidermidis and is absent in S. capitis. In this study, the role of S. epidermidis aap in enhancing biofilm formation and conferring an advantageous growth in PCs was investigated. MATERIALS AND METHODS:Biofilm formation assays of S. epidermidis 1457, S. epidermidis 1457∆aap, S. capitis 517 and S. capitis 517 carrying S. epidermidis aap (S. capitis 517/pRBaap) were performed in glucose-supplemented trypticase soy broth (TSBg) and PCs. Additionally, competition assays with paired cultures (1:1 ratio) of S. epidermidis and S. capitis strains were seeded in PCs, followed by determination of viable counts of each organism at the end of PC storage. RESULTS:Staphylococcus epidermidis aap had no effect on biofilm formation in TSBg. By contrast in PCs, S. epidermidis 1457 showed higher biofilm formation than S. epidermidis 1457∆aap (P < 0·05). Biofilm formation was also enhanced in S. capitis 517/pRBaap compared to S. capitis 517 (P = 0·054). Competition assays showed that S. epidermidis 1457 outcompeted S. capitis 517, and importantly, S. capitis 517/pRBaap outcompeted S. capitis 517 and S. epidermidis 1457∆aap. CONCLUSION:This study demonstrated that S. epidermidis aap plays a role in biofilm formation in PCs conferring an advantageous proliferation to skin flora bacteria in this milieu. The molecular mechanisms of action of Aap merit further investigation.


Vox Sang


Vox sanguinis


Alabdullatif M,Ramirez-Arcos S




Has Abstract


2019-01-01 00:00:00












  • Blood group ABH antigens on human cord red cells. Number of H antigenic sites and their distribution among different classes of membrane constituents.

    abstract::When the blood group H sites of cord erythrocytes obtained from newborn infants of groups O, A, B and AB were labelled specifically by incubation of the whole cells with the A1 gene dependent alpha-N-acetylgalactosaminyl transferase in the presence of UDP-N-acetyl [14C]-galactosamine, the incorporation of radioactivit...

    journal_title:Vox sanguinis

    pub_type: 杂志文章


    authors: Schenkel-Brunner H

    更新日期:1980-06-01 00:00:00

  • The effect of plasmapheresis on blood pressure in voluntary plasma donors.

    abstract:BACKGROUND AND OBJECTIVES:Donor plasmapheresis involves the removal of a weight-adjusted volume of plasma and the return of cellular components to the donor. Although plasma volume generally returns to normal, some residual effect on vital signs may be possible. This analysis was performed to determine the possible eff...

    journal_title:Vox sanguinis

    pub_type: 杂志文章


    authors: Rosa-Bray M,Wisdom C,Marier JF,Mouksassi MS,Wada S

    更新日期:2015-01-01 00:00:00

  • Resuscitation fluids.

    abstract:BACKGROUND AND OBJECTIVES:The aim of this review was to summarize the properties of the commonly available resuscitation fluids and highlight where knowledge of the characteristics of individual fluids might guide their use in clinical practice. Some of the current controversies surrounding resuscitation fluids are als...

    journal_title:Vox sanguinis

    pub_type: 杂志文章,评审


    authors: Grocott MP,Hamilton MA

    更新日期:2002-01-01 00:00:00

  • Factors related to allergic transfusion reactions and febrile non-haemolytic transfusion reactions in children.

    abstract:BACKGROUND AND OBJECTIVES:Allergic transfusion reactions (ATRs) and febrile non-haemolytic transfusion reactions (FNHTRs) are the two major types of transfusion-related adverse reactions (TRARs). Although prestorage leucocyte reduction and diversion of the first aliquot of blood (LR/D) could reduce FNHTRs and bacterial...

    journal_title:Vox sanguinis

    pub_type: 杂志文章


    authors: Yanagisawa R,Shimodaira S,Sakashita K,Hidaka Y,Kojima S,Nishijima F,Hidaka E,Shiohara M,Nakamura T

    更新日期:2016-05-01 00:00:00

  • The association of fever with transfusion-associated circulatory overload.

    abstract:BACKGROUND:Fever is described in transfusion-associated circulatory overload (TACO), reflecting either comprehensive haemovigilance or an inflammatory pathobiology (such as congestion-associated atheroma disruptions). METHODS:Hospital haemovigilance data (1/1/2010-31/12/2012) were reviewed for TACO cases (frequency an...

    journal_title:Vox sanguinis

    pub_type: 杂志文章


    authors: Parmar N,Pendergrast J,Lieberman L,Lin Y,Callum J,Cserti-Gazdewich C

    更新日期:2017-01-01 00:00:00

  • Use of Fab anti-IgG in phenotyping IgG-sensitised red cells.

    abstract::Previously described methods of phenotyping red cells sensitised with IgG using the indirect antiglobulin test required the dissociation of the coating protein. Based on an entirely different principle, Fab fragments of anti-human IgG (Fab anti-IgG) were used to block the antiglobulin binding sites on cell-bound IgG m...

    journal_title:Vox sanguinis

    pub_type: 杂志文章


    authors: Henry SM,Baird SJ,Woodfield DG

    更新日期:1987-01-01 00:00:00

  • A Swiss family showing independent segregation of the Lutheran and Swann genes.

    abstract::A family is reported in which the parents' mating is of the 'double back-cross' type with respect to the Sw, Lu and Se genes. The inheritance of these genes in the children shows, with reference to a possible linkage between Sw and the two other loci, four non-recombinants and three recombinants (or vice versa). The i...

    journal_title:Vox sanguinis

    pub_type: 杂志文章


    authors: Metaxas MN,Metaxas-Buehler M

    更新日期:1976-01-01 00:00:00

  • External quality assessment of human neutrophil antigen (HNA)-specific antibody detection and HNA genotyping from 2000 to 2012.

    abstract::Since 2000, Quality Assurance (QA) exercises for the detection and identification of granulocyte antibodies and DNA typing for human neutrophil antigens (HNA) have been distributed within the International Granulocyte Immunobiology Workshops, which are linked to International Society of Blood Transfusion. The exercise...

    journal_title:Vox sanguinis

    pub_type: 杂志文章


    authors: Lucas G,Porcelijn L,Fung YL,Green F,Reil A,Hopkins M,Schuller R,Green A,de Haas M,Bux J

    更新日期:2013-10-01 00:00:00

  • A family with allo-immune neonatal neutropenia: group-specific pathogenicity of maternal antibodies.

    abstract::A family is presented, in which the mother developed strong neutrophil granulocyte-specific antibodies with the specificity anti-NA. The first and third children, both NA1-positive, suffered from severe transient neonatal neutropenia, but the second child, NA1-negative, did not. Anti-NA1 antibodies were detectable in ...

    journal_title:Vox sanguinis

    pub_type: 杂志文章


    authors: Verheugt FW,van Noord-Bokhorst JC,von dem Borne AE,Engelfriet CP

    更新日期:1979-01-01 00:00:00

  • Analbuminemia: report of a case and review of the literature.

    abstract::Idiopathic analbuminemia was fortuitously detected in a 30-year-old Italian woman. No associated disease condition could be revealed in this patient, the only consistent finding being a slight tendency to develop ankle edema. Asthenia and a moderate hypotension were also recorded. Trace amounts (5.2 mg/100 ml) of seru...

    journal_title:Vox sanguinis

    pub_type: 杂志文章,评审


    authors: Dammacco F,Miglietta A,D'Addabbo A,Fratello A,Moschetta R,Bonomo L

    更新日期:1980-09-01 00:00:00

  • Detection of early anti-p24 HIV responses in EIA- and immunoblot-negative individuals. Implications for confirmatory testing.

    abstract::A sensitive and specific radioimmunoprecipitation assay was developed for the detection and analysis of anti-HIV antibody response in human sera with the use of 125I-labelled purified HIV proteins with subsequent sodium-dodecylsulfate gel electrophoresis (125I-RIPA). The 125I-RIPA was shown to be as specific but at le...

    journal_title:Vox sanguinis

    pub_type: 杂志文章


    authors: Huisman JG,Winkel IN,Lelie PN,Tersmette M,Goudsmit J,Miedema F

    更新日期:1987-01-01 00:00:00

  • Screening blood donors for Plasmodium falciparum malariae. Application of ready-to-use homologous antigens.

    abstract::Prevention of post-transfusion malaria remains a worrying problem. Until now it was very difficult to obtain homologous antigens, and immunologic methods used to detect malaria were expensive. We tested the ready-to-use Plasmodium falciparum prepared slides using the IFA (indirect fluorescent antibody) test on 866 sel...

    journal_title:Vox sanguinis

    pub_type: 杂志文章


    authors: Deroff P,Reguer M,Simitzis AM,Boudon A,Saleun JP

    更新日期:1983-01-01 00:00:00

  • Evaluation of HLA-haplotype disparate parental marrow grafts depleted of T lymphocytes by differential agglutination with a soybean lectin and E-rosette depletion for the treatment of severe combined immunodeficiency.

    abstract::The factors that impact upon successful bone marrow transplantation leading to immunologic reconstitution in severe combined immune deficiency (SCID). Wiskott-Aldrich syndrome, and in other lethal congenital immunodeficiencies are reviewed. Evidence is presented that graft-versus-host disease (GVHD) can be abrogated b...

    journal_title:Vox sanguinis

    pub_type: 杂志文章


    authors: O'Reilly RJ,Brochstein J,Collins N,Keever C,Kapoor N,Kirkpatrick D,Kernan N,Dupont B,Burns J,Reisner Y

    更新日期:1986-01-01 00:00:00

  • Inactivation of hepatitis A virus by pasteurization and elimination of picornaviruses during manufacture of factor VIII concentrate.

    abstract::Hepatitis A virus (HAV) infections have been reported among hemophiliacs who received factor VIII concentrates which had been purified by ion-exchange chromatography and treated by the solvent detergent (SD) method. Since the virus inactivation procedure of our manufacturing process is heat treatment of the stabilized...

    journal_title:Vox sanguinis

    pub_type: 杂志文章


    authors: Hilfenhaus J,Nowak T

    更新日期:1994-01-01 00:00:00

  • KEL*02 alleles with alterations in and around exon 8 in individuals with apparent KEL:1,-2 phenotypes.

    abstract:BACKGROUND AND OBJECTIVES:Antibodies to antigens in the Kell blood group system, especially anti-KEL1, are involved in both haemolytic disease of the newborn and foetus and haemolytic transfusion reactions. Correct typing results are important and discrepancies between serologic and genetic typing must be resolved. Her...

    journal_title:Vox sanguinis

    pub_type: 杂志文章


    authors: Wester ES,Steffensen R,Ligthart PC,Vad J,de Haas M,Storry JR,Olsson ML

    更新日期:2010-08-01 00:00:00

  • The non-Mendelian inheritance of Lewis-c blood group substance, as demonstrated in the case of a Bombay, Le(a-b-c-) saliva.

    abstract::A Bombay, Le(a-b-) saliva was shown to lack Pneumococcus type XIV activity, an unusual situation, since this sample should be rich in this precursor to the ABO blood group substances. However, the sample was found to contain a new serological specificity, Le-c. It is argued that simple Mendelian inheritance does not o...

    journal_title:Vox sanguinis

    pub_type: 杂志文章


    authors: Savvas RS

    更新日期:1975-01-01 00:00:00

  • Placental transfer of Rh antibody (anti-D IgG) during pregnancy.

    abstract::Anti-D levels in blood taken immediately postpartum from Rh-negative women who have been given antenatal Rh immune globulin and the anti-D levels in the cord blood of their babies were measured semiquantitatively by a low ionic polybrene Auto Analyzer technique. When both mother and baby were Rh-negative a comparison ...

    journal_title:Vox sanguinis

    pub_type: 杂志文章


    authors: Pollock JM,Bowman JM

    更新日期:1982-01-01 00:00:00

  • Maternal-fetal hemorrhage: a reappraisal.

    abstract:PURPOSE:To determine the frequency of maternal-fetal hemorrhage at or above 1 microliter of maternal whole blood. METHODS:Seventy-three mothers whose red blood cells bore an Rh antigen (Rh D, Rh c, Rh E) that was absent on red blood cells of their newborns were identified and a new cytological method, the Kleihauerimm...

    journal_title:Vox sanguinis

    pub_type: 杂志文章


    authors: Brossard Y,Pons JC,Jrad I,van Nifterik J,Gillot R,Saure C,Le Gaudion M,Richard A,Papiernik E

    更新日期:1996-01-01 00:00:00

  • Allelic subdivisions of the HLA-B12-Bw21 complex: association with Bw4 and Bw6.

    abstract::Allelic subdeterminants (or splits) of the B12-Bw21 complex were characterized by the use of highly selected antisera and cell donors. The B12 antigen was separated into two major components, Bw44 and Bw45; and possibly a third. It was shown that Bw44 is primarily an antigen of whites, and Bw45 of blacks. Of the 68 pe...

    journal_title:Vox sanguinis

    pub_type: 杂志文章


    authors: Mittal KK

    更新日期:1980-01-01 00:00:00

  • Intraoperative autologous blood recovery in prostate cancer surgery: in vivo validation using a tumour marker.

    abstract:BACKGROUND AND OBJECTIVE:Intraoperative autologous blood recovery during radical retro pubic prostatectomy has the potential of contamination with tumour cells. Its safety is proved by similar survival rates between allogeneic and autologous transfusion to oncology patients without standardization. Silencing of the gen...

    journal_title:Vox sanguinis

    pub_type: 杂志文章


    authors: Poli M,Camargo A,Villa L,Moura R,Colella R,Deheinzelin D

    更新日期:2008-11-01 00:00:00

  • Nonradioactive chromium technique for red cell labeling.

    abstract::Following transfusion, red cells labeled with nonradioactive chromium (52Cr) can be detected in the circulation using atomic absorption spectrophotometry. Both in rhesus monkeys and in human subjects, the survival of red cells labeled with 52Cr was found to be insignificantly different from that of cells labeled with ...

    journal_title:Vox sanguinis

    pub_type: 杂志文章


    authors: Sioufi HA,Button LN,Jacobson MS,Kevy SV

    更新日期:1990-01-01 00:00:00

  • Iron deficiency among French whole-blood donors: first assessment and identification of predictive factors.

    abstract:BACKGROUND:The objectives of this study are to estimate the prevalence of iron deficiency (ID) among French whole-blood (WB) donors to identify factors associated with ID and to generate decision trees. STUDY DESIGN AND METHODS:A prospective National multicentre study was performed on WB donors from March 11, to April...

    journal_title:Vox sanguinis

    pub_type: 杂志文章


    authors: Fillet AM,Martinaud C,Malard L,Le Cam S,Hejl C,Chenus F,Woimant G,Chueca M,Jacquot E,Besiers C,Morel P,Djoudi R,Garrabé E,Gross S

    更新日期:2021-01-01 00:00:00

  • Molecular genetic analysis of the ABO blood group system: 3. A(X) and B(A) alleles.

    abstract::We have employed a PCR approach to determine the nucleotide sequences of the coding region in the last two coding exons of the histo-blood group ABO genes from one A(X) and one B(A) individual. Compared with A1 alleles, the (A(X)) allele has a single nucleotide substitution (T-->A at nucleotide 646) resulting in an am...

    journal_title:Vox sanguinis

    pub_type: 杂志文章


    authors: Yamamoto F,McNeill PD,Yamamoto M,Hakomori S,Harris T

    更新日期:1993-01-01 00:00:00

  • Sensitivity of assays for the detection of HPA-1a antibodies: results of an international workshop demonstrating the impact of cation chelation from integrin αIIbβ3 on three widely used assays.

    abstract:BACKGROUND AND OBJECTIVES:HPA-1a antibodies account for 70-80% of cases of fetal-neonatal alloimmune thrombocytopenia (FNAIT) in Caucasians. However, numerous workshops have demonstrated variability in their detection. We recently showed that exposure of αIIbβ3 to ethylene diamine tetraacetic acid (EDTA) affected bindi...

    journal_title:Vox sanguinis

    pub_type: 杂志文章


    authors: Allen DL,Metcalfe P,Kaplan C,Kekomaki R,de Haas M,Yusuf R,Ouwehand WH

    更新日期:2013-08-01 00:00:00

  • Loss of phosphotyrosine phosphatase activity and changes in the tyrosine phosphorylation state of proteins after storage of sheep platelets in plasma or Seto solution at 4 degrees C.

    abstract:BACKGROUND AND OBJECTIVES:During platelet storage an array of deleterious changes occur, through mechanisms not fully understood, which impair platelet haemostasis. Transfused platelets should maintain the integrated networks of signalling pathways that regulate platelet activation and functionality. We hypothesized th...

    journal_title:Vox sanguinis

    pub_type: 杂志文章


    authors: Hernández-Hernández A,Sánchez-Bernal C,Rodríguez MC,Gómez FP,Llanillo M,Sánchez-Yagüe J

    更新日期:2001-11-01 00:00:00

  • Biochemical and biological properties of an alpha 1-antitrypsin concentrate.

    abstract::alpha 1-Antitrypsin (AAT) has been purified from human plasma supernatant A (equivalent to COHN fraction II + III) by a large-scale chromatographic procedure involving anion-exchange adsorption on DEAE Sepharose CL-6B fast flow and size-exclusion chromatography on Sephacryl S-200. Before freeze-drying, the liquid conc...

    journal_title:Vox sanguinis

    pub_type: 杂志文章


    authors: Burnouf T,Constans J,Clerc A,Descamps J,Martinache L,Goudemand M

    更新日期:1987-01-01 00:00:00

  • Blood transfusion in preterm infants improves intestinal tissue oxygenation without alteration in blood flow.

    abstract:BACKGROUND AND OBJECTIVE:The objective of the study was to investigate the splanchnic blood flow velocity and oximetry response to blood transfusion in preterm infants according to postnatal age. MATERIALS AND METHODS:Preterm infants receiving blood transfusion were recruited to three groups: 1-7 (group 1; n = 20), 8-...

    journal_title:Vox sanguinis

    pub_type: 杂志文章


    authors: Banerjee J,Leung TS,Aladangady N

    更新日期:2016-11-01 00:00:00

  • Sexual risk behaviour and donor deferral in Europe.

    abstract::One of the most controversial policies in blood transfusion worldwide is the permanent deferral from donating blood of men with sexual contacts to other men (MSM). This policy was implemented for safety reasons as sex between men is known to be a high risk factor for acquiring severe infectious diseases transmissible ...

    journal_title:Vox sanguinis

    pub_type: 杂志文章


    authors: Offergeld R,Kamp C,Heiden M,Norda R,Behr-Gross ME

    更新日期:2014-11-01 00:00:00

  • Estimated risk of transmission of hepatitis C virus by blood transfusion.

    abstract:OBJECTIVE:The risk of transmitting hepatitis C (HCV) by transfusion of anti-HCV-negative screened blood was estimated for the blood donor population of Baden-Württemberg (southwestern Germany). METHODS:The data from the blood donors screened for anti-HCV and for HBsAg during 1990-1995 were analyzed. RESULTS:The preva...

    journal_title:Vox sanguinis

    pub_type: 杂志文章,多中心研究


    authors: Koerner K,Cardoso M,Dengler T,Kerowgan M,Kubanek B

    更新日期:1998-01-01 00:00:00

  • Recurrent hepatitis caused by two viruses acquired simultaneously during blood transfusion.

    abstract::We here report 2 patients who have acquired two consecutive episodes each of acute hepatitis, within 6 months after blood transfusion. The clinical and biochemical course was typical of recurrent acute viral hepatitis, and the patients recovered completely after each episode. The first infection was probably caused by...

    journal_title:Vox sanguinis

    pub_type: 杂志文章


    authors: Ben-Dov I,Fainaru M

    更新日期:1980-10-01 00:00:00