Serological characterization of murine monoclonal antibodies directed against acquired B red cells.

abstract：:Because substrate specificities differ between proteolytic enzymes and because knowledge of the optimal enzyme activity levels is necessary in order to standardize procedures used in antibody screening, a study was made of the best common assay method for the routinely employed enzymes bromelain, papain and ficin. Cas...

journal_title：Vox sanguinis

authors： Ogasawara K,Mazda T

更新日期：1989-01-01 00:00:00

abstract：BACKGROUND AND OBJECTIVES:To prove the feasibility of a semi-automated cross-match procedure using a commercially available solid-phase microplate test and standard laboratory equipment. MATERIAL AND METHODS:The new procedure was evaluated against the conventional spin tube technique and the gel centrifugation system....

journal_title：Vox sanguinis

authors： Ostendorf N,Niefhoff D,Cassens U,Sibrowski W

更新日期：2001-05-01 00:00:00

abstract：:A competitive inhibition assay for functional fibronectin (Fn), based on ELISA technology, is described. The assay measures Fn's physiologic ability to bind to denatured collagen (gelatin). Affinity-purified Fn inhibits the binding of alkaline phosphatase coupled Fn to gelatin-coated wells of a microtiter plate in a c...

journal_title：Vox sanguinis

authors： Doran JE,Callaway BD,Reese AC,Wynn JJ,Mansberger AR

更新日期：1983-01-01 00:00:00

abstract：:Articles on the appropriateness of intention-to-treat (ITT) vs. as-treated (AT) analyses of randomized controlled trials (RCTs) have highlighted issues relevant to drug trials, such as approaches suitable for 'explanatory' vs. 'pragmatic' RCTs. These considerations are less relevant to transfusion medicine RCTs, espec...

journal_title：Vox sanguinis

authors： Vamvakas EC

更新日期：2008-10-01 00:00:00

abstract：BACKGROUND AND OBJECTIVES:Weak ABO variants may escape tests using unlicensed sera. MATERIALS AND METHODS:Prior to transfusion, ABO grouping was performed using an automated system and in-house diluted sera, and manual and bedside test techniques. Genotyping and sequencing were performed using standard methods. RESUL...

journal_title：Vox sanguinis

authors： Pruss A,Heymann GA,Braun J,Kiesewetter HH,Salama A

更新日期：2006-04-01 00:00:00

abstract：BACKGROUND:Human platelet growth factors (HPGF) are essential for tissue regeneration and may replace fetal bovine serum (FBS) in cell therapy. No method for the manufacture of standardized virally inactivated HPGF has been developed yet. STUDY DESIGN AND METHODS:Platelet concentrates (PC) were subjected to solvent/de...

journal_title：Vox sanguinis

authors： Su CY,Kuo YP,Lin YC,Huang CT,Tseng YH,Burnouf T

更新日期：2009-08-01 00:00:00

abstract：BACKGROUND:Recent changes have resulted in the loss of 4% of the donor panel in the Republic of Ireland and 3% in Northern Ireland. In order to increase the number of donors in these two regions, it is important that transfusion service providers explore and understand the reasons, which prevent individuals from donati...

journal_title：Vox sanguinis

authors： Harrington M,Sweeney MR,Bailie K,Morris K,Kennedy A,Boilson A,O'Riordan J,Staines A

更新日期：2007-05-01 00:00:00

abstract：:Sera from 2,829 participants in a population health survey were tested for the presence of a heterophilic antibody, detectable by immunofluorescence. The antibody was found in 9.8% of the sera from women and in 12.4% of the sera from men. The age-specific prevalence of the antibody was relatively constant in women but...

journal_title：Vox sanguinis

authors： Hawkins BR,Saueracker GC,Dawkins RL,Davey MG,O'Connor KJ

更新日期：1980-12-01 00:00:00

abstract：:Isoagglutinins were investigated following ABO-incompatible bone marrow transplantation. In major incompatibility anti-A tends to require more time to disappear than anti-B. Correspondingly, A erythrocytes require more time to become demonstrable. There is no such difference in major plus minor incompatibility. In min...

journal_title：Vox sanguinis

authors： Wernet D,Mayer G

更新日期：1992-01-01 00:00:00

abstract：BACKGROUND AND OBJECTIVES:National guidelines for monitoring bacterial contamination of blood components were introduced in Germany in 1997. Between 1998 and 2002, numerous measures were implemented to prevent bacterial contamination. This study investigates their impact on contamination rates. MATERIALS AND METHODS:C...

journal_title：Vox sanguinis

authors： Walther-Wenke G,Wirsing von König CH,Däubener W,Heiden M,Hoch J,Hornei B,Volkers P,Working Party on Bacteria Safety in Transfusion Medicine, Advisory Board of the German Ministry of Health, Berlin.

更新日期：2011-05-01 00:00:00

abstract：:Bivalent 7S subunits were obtained from 7 purified IgM human monoclonal anti-D antibodies with 33-63% of the total protein-retaining functional binding activity. The number of sites per red cell recognized by these subunits ranged between 9,400 and 28,500. The values are taken to indicate that a number of different ep...

journal_title：Vox sanguinis

authors： Hughes-Jones NC,Gorick BD,Beale D

更新日期：1990-01-01 00:00:00

abstract：BACKGROUND AND OBJECTIVES:While blood is flowing within a transfusion-warming device, the blood temperature is usually less than that applied externally. If the flow is temporarily stopped, the temperature can rise above 37 degrees C in some warming devices. We sought to determine whether temperatures near 45 degrees C...

journal_title：Vox sanguinis

authors： Eastlund T,Van Duren A,Clay ME

更新日期：1999-01-01 00:00:00

abstract：BACKGROUND AND OBJECTIVES:The last two decades have seen major developments in genotyping assays to facilitate the procurement of red blood cell units to alloimmunized patients. To make genotyping faster, simpler and less costly, a nanotechnology approach based on metal/silica fluorescent nanoparticles and a polymer-ba...

journal_title：Vox sanguinis

authors： Brouard D,Ratelle O,Perreault J,Boudreau D,St-Louis M

更新日期：2015-02-01 00:00:00

abstract：BACKGROUND:A disposable set for platelet concentrate (PC) preparation by the buffy coat method allows pooling of buffy coats, centrifugation and cell separation with in-line leucocyte filtration. This study compares three commercially available pooling sets in combination with INTERCEPT pathogen inactivation (PI). MAT...

journal_title：Vox sanguinis

authors： Feys HB,Devloo R,Sabot B,Coene J,Compernolle V

更新日期：2018-05-24 00:00:00

abstract：:Pst I endonuclease fragment length polymorphism of the antithrombin III probe was studied in a French population. Among 25 subjects tested, 15 were found heterozygous at the Pst I site, so that this polymorphism might serve as a useful markers of antithrombin III gene in families with antithrombin III deficiency. ...

journal_title：Vox sanguinis

authors： Le Paslier D,Rochu D,Lucotte G

更新日期：1985-01-01 00:00:00

abstract：BACKGROUND AND OBJECTIVES:Allergic transfusion reactions (ATRs) and febrile non-haemolytic transfusion reactions (FNHTRs) are the two major types of transfusion-related adverse reactions (TRARs). Although prestorage leucocyte reduction and diversion of the first aliquot of blood (LR/D) could reduce FNHTRs and bacterial...

journal_title：Vox sanguinis

authors： Yanagisawa R,Shimodaira S,Sakashita K,Hidaka Y,Kojima S,Nishijima F,Hidaka E,Shiohara M,Nakamura T

更新日期：2016-05-01 00:00:00

abstract：BACKGROUND AND OBJECTIVES:Pr1,2,3, PrM, Sa and Sia-l1, -b1, -lb1 are sialic acid (NeuNAc)-dependent antigens recognized by human cold agglutinins. Pr and Sa antigens are the O-glycans of glycophorins containing alpha 2,3NeuNAc (to galactose) and/or alpha 2,6NeuNAc (to galactosamine). The antigens of the Sia-l1, -b1, -l...

journal_title：Vox sanguinis

authors： Roelcke D,Hack H,Kreft H,Gross HJ

更新日期：1998-01-01 00:00:00

abstract：:BOW is a 'new' low-frequency red-cell antigen, detected in 2 unrelated English blood donors, that is sensitive to alpha-chymotrypsin and pronase. Anti-BOW is present in many polyspecific reagents used to define low-frequency antigens. Red-cell groups of the proposita, R.B., and her family show that the BOW blood group...

journal_title：Vox sanguinis

authors： Chaves MA,Leak MR,Poole J,Giles CM

更新日期：1988-01-01 00:00:00

abstract：:We simulated the use of massive volumes of crystalloid fluids as a treatment of acute plasma loss in a standardized experimental model and studied the factors determining the retention or excretion of the resulting acute hypoproteinemic fluid overload, its distribution within the body, and its treatment with concentra...

journal_title：Vox sanguinis

authors： Pappova E,Bachmeier W,Crevoisier JL,Kollar J,Kollar M,Tobler P,Zahler HW,Zaugg D,Lundsgaard-Hansen P

更新日期：1977-01-01 00:00:00

abstract：:Blood plasma and factor VIII concentrate have been fractionated by continuous free film electrophoresis in an apparatus which was designed to overcome the problems of overheating usually inherent in this technique. Results show that complete separation of plasma proteins is unlikely to be obtained by a single passage ...

journal_title：Vox sanguinis

authors： Austen DE,Cartwright T,Dickerson CH

更新日期：1983-03-01 00:00:00

abstract：:The Gm allotype constitution of the autoantibody molecules and the serum Gm phenotypes were determined in 19 patients with autiommune hemolytic anemia of IgG type. The results showed that the known heterogeneity of the antierythrocyte autoantibodies in this disease concerns not only the immunoglobulin class and the is...

journal_title：Vox sanguinis

authors： Le Petit JC,Rivat L,François N,Ropartz C,Brizard CP

更新日期：1976-01-01 00:00:00

abstract：BACKGROUND AND OBJECTIVES:The study was designed to evaluate whether volume replacement following blood donation can prevent arterial hypotension in autologous blood donors with cardiovascular disease. MATERIALS AND METHODS:One hundred nineteen autologous blood donors with known cardiovascular disease were randomly al...

journal_title：Vox sanguinis

authors： Kasper SM,Dahlmann H,Mellinghoff H,Ellering J,Baumann M,Buzello W

更新日期：1998-01-01 00:00:00

abstract：:Paraformaldehyde-fixed platelets from normal donors were used for detection of antibody to platelet by in vitro sensitization (indirect method) utilizing rabbit anti-human IgG heavy chain specific antibody-coated polyacrylamide gels (Immunobeads). The sensitized platelets formed rosettes with Immunobeads and the posit...

journal_title：Vox sanguinis

authors： Salmassi S,Yokoyama MM,Maples JA,Matsui Y

更新日期：1980-11-01 00:00:00

abstract：:There is an ongoing debate whether platelet concentrates (PCs) prepared from either whole-blood donations or by plateletpheresis are superior. Usage of these two product types varies greatly between countries and individual institutions. Some use mainly apheresis PCs; others prefer pooled PCs which are produced from w...

journal_title：Vox sanguinis

authors： Schrezenmeier H,Seifried E

更新日期：2010-07-01 00:00:00

abstract：:A number of parasitic diseases are known, or suspected to be transmitted by blood transfusion. Of greatest concern are malaria and Chagas' disease, but babesia, leishmania and toxoplasmosis also offer risk in particular locations or circumstances. Some of these parasites may be imported into non-endemic areas as a res...

journal_title：Vox sanguinis

authors： Dodd RY

更新日期：1998-01-01 00:00:00

abstract：:An antibody to a high frequency antigen, made in a WES+ Black antenatal patient (Wash.), failed to react with the red cells of a presumed WES+ homozygote and is, therefore, probably antithetical to anti-WES. Like anti-WES, it reacted with papain, ficin, trypsin or neuraminidase treated cells but not with alpha-chymotr...

journal_title：Vox sanguinis

authors： Daniels GL,Green CA,Darr FW,Anderson H,Sistonen P

更新日期：1987-01-01 00:00:00

abstract：:beta 2-Microglobulin is a low molecular weight protein with sequence homology to immunoglobulins. As a portion of the HLA complex this protein is an important cell-surface structure. Under normal conditions beta 2-microglobulin is synthesized and shed by many cells, particularly lymphocytes, and is detectable in the c...

journal_title：Vox sanguinis

authors： Bernier GM

更新日期：1980-06-01 00:00:00

abstract：BACKGROUND AND OBJECTIVES:Since July 1 1999, four laboratories in the Netherlands have been routinely screening plasma minipools for the release of labile blood components utilizing hepatitis C virus nucleic acid amplification technology (HCV NAT). This report describes the performance evaluation of the HCV NAT method ...

journal_title：Vox sanguinis

authors： Cuijpers HT,Molijn MH,Bos HJ,Peeters AP,van der Poel CL,Lelie PN

更新日期：2001-07-01 00:00:00

abstract：:We tested serum samples from 11 HIV-seropositive and 32 HIV-seronegative Italian patients with hemophilia A or B for the presence of lymphocytotoxic antibodies (LCTAs). No patients had the acquired immune deficiency syndrome and all had received over many years heat-untreated commercial clotting factors. LCTAs directe...

journal_title：Vox sanguinis

authors： Conte R,Giovanardi L,Tazzari PL,De Rosa V,Rodorigo G,Re MC

更新日期：1988-01-01 00:00:00

abstract：:The presence of the glycoproteins haptoglobin and hemopexin in human plasma albumin (HPA) solutions were demonstrated to be responsible for the formation of polymers and aggregates during heat treatment for 10 h at 60 degrees C. Apart from haptoglobin and hemopexin three other contaminating proteins were identified as...

journal_title：Vox sanguinis

authors： Jensen LB,Dam J,Teisner B

更新日期：1994-01-01 00:00:00