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GTP and cytosol stimulate phosphoinositide hydrolysis in isolated platelet membranes.

Abstract：

:Hydrolysis of polyphosphoinositides by phospholipase C was examined in isolated membranes prepared from [32P]labelled platelets. In the presence of GTP gamma S, thrombin increased the release of inositol triphosphate and inositol biphosphate approximately 500%. GTP gamma S alone stimulated release 2 fold. Maximal activation of thrombin-induced phosphoinositide hydrolysis was observed at 10 uM GTP. Although addition of calcium had no effect, 2 mM EGTA completely inhibited inositolphosphate release. Addition of high speed supernatant to [32P]labelled membranes stimulated the release of inositolphosphates. This hydrolysis was further enhanced by the addition of GTP. These data demonstrate that the breakdown of polyphosphoinositides in isolated platelet membranes is dependent on GTP and stimulated by platelet cytosol.

journal_name

Biochem Biophys Res Commun

journal_title

Biochemical and biophysical research communications

authors

Baldassare JJ,Fisher GJ

doi

10.1016/0006-291x(86)91150-2

subject

Has Abstract

pub_date

1986-06-13 00:00:00

pages

801-5

issue

2

eissn

0006-291X

issn

1090-2104

pii

0006-291X(86)91150-2

journal_volume

137

pub_type

杂志文章

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