Roles for LPS-dependent interaction and relocation of TLR4 and TRAM in TRIF-signaling.

abstract：:The recently detected Rieske iron-sulfur center in the membrane of the thermoacidophilic archaeon Sulfolobus acidocaldarius (Anemüller et al., 1993, FEBS Lett. 318, 61-64) was further characterized by EPR spectroscopy, coupled to redox-potentiometry and functional studies. The reduction potential is pH-dependent above...

journal_title：Biochemical and biophysical research communications

authors： Anemüller S,Schmidt CL,Schäfer G,Bill E,Trautwein AX,Teixeira M

更新日期：1994-07-15 00:00:00

abstract：:We have examined the steady-state levels of intermediate filament mRNA in amyotrophic lateral sclerosis using the RNAse protection assay (NFL, NFM, NFH; corrected against GAPDH) or by PCR (peripherin, alpha-internexin, nestin, and vimentin; corrected against beta-actin). Significant elevations of NFL and peripherin mR...

journal_title：Biochemical and biophysical research communications

authors： Strong MJ,Leystra-Lantz C,Ge WW

更新日期：2004-04-02 00:00:00

abstract：:A protein of M(r) 59,000 (p59) was recently cloned and identified as a Heat shock protein Binding Immunophilin (p59/HBI). It participates to the heterooligomeric, non-DNA binding form of steroid receptors, in association with the heat shock protein of M(r) 90,000 (hsp90). It binds the immunosuppressants FK506 and rapa...

journal_title：Biochemical and biophysical research communications

authors： Le Bihan S,Renoir JM,Radanyi C,Chambraud B,Joulin V,Catelli MG,Baulieu EE

更新日期：1993-09-15 00:00:00

abstract：:A phosphate buffer containing a mixture of glucose oxidase, acrylic acid derivatives, N,N'-1,2 dihydroxy-ethylene-bis(acrylamide), N,N'-(methylene)-bisacrylamide and surface-modified silica was radically polymerized with (NH4)2S2O8. The polymer formed a thin layer around the silica beads. After sieving of these polyme...

journal_title：Biochemical and biophysical research communications

authors： Burow M,Minoura N

更新日期：1996-10-14 00:00:00

abstract：:TGFbeta is of crucial importance during transdifferentiation of resting retinoid-storing hepatic stellate cells (HSC) to extracellular matrix producing myofibroblasts (MFB) and consequently, inhibition of TGFbeta signal transduction is an effective means for preventing experimental fibrosis. We have shown that isolate...

journal_title：Biochemical and biophysical research communications

authors： Dooley S,Streckert M,Delvoux B,Gressner AM

更新日期：2001-05-11 00:00:00

abstract：:We report here that at least seven low Mr GTP-binding proteins (range 21.5 to 29 kDa) are associated with the membranes of zymogen granules from rat pancreas. GTP binding proteins of similar Mr but in different relative proportions were found in the cytosolic fraction. Treatment of intact granules with either trypsin ...

journal_title：Biochemical and biophysical research communications

authors： Padfield PJ,Jamieson JD

更新日期：1991-01-31 00:00:00

abstract：:Endothelin (ET)-1 and big ET-1 both caused contraction of isolated porcine coronary arteries, but the potency of big ET-1 was 1/100-1/200 that of ET-1. These responses were independent of the vascular endothelium. Phosphoramidon blocked the vasoconstriction caused by 30 nM big ET-1, but was ineffective on the action o...

journal_title：Biochemical and biophysical research communications

authors： Fukuroda T,Noguchi K,Tsuchida S,Nishikibe M,Ikemoto F,Okada K,Yano M

更新日期：1990-10-30 00:00:00

abstract：:We have cloned two cDNA isoforms as well as genomic sequences of the mouse Prx V gene and characterized their molecular genetic features. Two isoforms of the mouse Prx V cDNA were identified from liver and testis. The testis-originated long transcripts had extra 1164-bp 5'-UTR sequences compared to the liver-originate...

journal_title：Biochemical and biophysical research communications

authors： Lee TH,Kim SJ,Kang SW,Lee KK,Rhee SG,Yu DY

更新日期：2000-04-13 00:00:00

abstract：:AMP-activated protein kinase (AMPK) is an important sensor of cellular energy status, and is involved in cell growth and autophagy through mammalian target of rapamycin complex 1 (mTORC1). Carbonyl cyanide m-chlorophenylhydrazone (CCCP), a mitochondrial uncoupler, leads to AMPK activation and Parkin-dependent mitophag...

journal_title：Biochemical and biophysical research communications

authors： Kwon KY,Viollet B,Yoo OJ

更新日期：2011-12-16 00:00:00

abstract：:Green fluorescent protein (GFP)-tagged phospholipase C (PLC)-delta1 and its mutants were expressed in Madin-Darby canine kidney (MDCK) cells. GFP-PLC-delta1 or the GFP-tagged pleckstrin homology (PH) domain of PLC-delta1 itself was found to be predominantly localized at the plasma membrane. The DeltaPH mutant or a sit...

journal_title：Biochemical and biophysical research communications

authors： Fujii M,Ohtsubo M,Ogawa T,Kamata H,Hirata H,Yagisawa H

更新日期：1999-01-19 00:00:00

abstract：:Pancreatic carcinoma is characterized by a poor prognosis and lack of response to conventional therapy. The regulatory mechanisms for the rapid proliferation of pancreatic cancer cells and the particular aggressiveness of this cancer are still not fully understood. In mammalian cells, three MAPK families including ERK...

journal_title：Biochemical and biophysical research communications

authors： Ding XZ,Adrian TE

更新日期：2001-03-30 00:00:00

abstract：:The ability to identify apoptotic cells within a complex population is crucial in the research and diagnosis of normal physiology and disease states. The Cellscan mark S (CS-S) cytometer was used in this study to detect intracellular fluorescence intensity and polarization (FI and FP) in several well-established model...

journal_title：Biochemical and biophysical research communications

authors： Zurgil N,Schiffer Z,Shafran Y,Kaufman M,Deutsch M

更新日期：2000-02-05 00:00:00

abstract：:Combined-modality treatment has improved the outcome in cases of various solid tumors, and radiosensitizers are used to enhance the radiotherapeutic efficiency. Rosiglitazone, a synthetic ligand of peroxisome proliferator-activated receptors gamma used in the treatment of type-2 diabetes, has been shown to reduce tumo...

journal_title：Biochemical and biophysical research communications

authors： Chiu SJ,Hsaio CH,Tseng HH,Su YH,Shih WL,Lee JW,Chuah JQ

更新日期：2010-04-09 00:00:00

abstract：:A 48-h incubation of cultured human fibroblasts with 5 x 10(-5) M oleic acid or polyunsaturated fatty acids (PUFA) from the (n-6) (linoleic, gamma-linolenic and arachidonic acids) or (n-3) (alpha-linolenic and eicosapentaenoic acids) series resulted in an enrichment of the cells with the introduced fatty acid. Cell en...

journal_title：Biochemical and biophysical research communications

authors： Mazière C,Conte MA,Degonville J,Ali D,Mazière JC

更新日期：1999-11-01 00:00:00

abstract：:Platelet-derived growth factor (PDGF) is a potent mesenchymal cell mitogen and chemoattractant involved in the pathogenesis of fibroproliferative diseases. There are four known PDGF ligand isoforms designated A-D, two of which, C and D, were only recently discovered. We have identified a splicing variant in the PDGF-D...

journal_title：Biochemical and biophysical research communications

authors： Zhuo Y,Hoyle GW,Zhang J,Morris G,Lasky JA

更新日期：2003-08-15 00:00:00

abstract：:HIV-1 Vpr is a protein with multiple functions. It has been suggested that such pleiotropic effects by a viral protein may be mediated by its association with viral and cellular proteins or through modulation of expression of specific cellular genes. To address this, we used cDNA microarray techniques to analyze the r...

journal_title：Biochemical and biophysical research communications

authors： Janket ML,Manickam P,Majumder B,Thotala D,Wagner M,Schafer EA,Collman RG,Srinivasan A,Ayyavoo V

更新日期：2004-02-20 00:00:00

abstract：:A glycerol kinase (Gyk) knock-out (KO) mouse model permits improved understanding of glycerol kinase (GK) deficiency (GKD) pathogenesis, however, early death of affected mice limits its utility. The purpose of this work was to delay death of affected males to investigate thoroughly their phenotypes. An adenoviral vect...

journal_title：Biochemical and biophysical research communications

authors： Kuwada N,Nagano K,MacLennan N,Havens J,Kumar M,Dipple KM,McCabe ER

更新日期：2005-09-16 00:00:00

abstract：:As part of an effort to identify genes potentially involved in the Down Syndrome pathogenesis, in this paper we report the identification and characterization of a new human gene (named SH3BGRL), which shows a high homology to the SH3BGR gene, previously mapped to the Down Syndrome region of chromosome 21. The SH3BGRL...

journal_title：Biochemical and biophysical research communications

authors： Egeo A,Mazzocco M,Arrigo P,Vidal-Taboada JM,Oliva R,Pirola B,Giglio S,Rasore-Quartino A,Scartezzini P

更新日期：1998-06-18 00:00:00

abstract：:The dystrophin-associated protein complex (DAPC), consisting of dystrophin, dystroglycans, sarcoglycans, dystrobrevins and syntrophins, provides a linkage between the cytoskeleton and the extracellular matrix. The disruption of DAPC leads to Duchenne/Becker muscular dystrophy and other neuromuscular diseases. Although...

journal_title：Biochemical and biophysical research communications

authors： Romo-Yáñez J,Montañez C,Salazar-Olivo LA

更新日期：2011-01-14 00:00:00

abstract：:A complete understanding of bitterness perception requires identification of cognate bitter substances for all human bitter taste receptors (TAS2Rs). However, so far, no agonists have been identified for five of the 25 TAS2Rs, i.e., TAS2R41, TAS2R42, TAS2R45, TAS2R48 and TAS2R60. Due to substantial genetic variability...

journal_title：Biochemical and biophysical research communications

authors： Thalmann S,Behrens M,Meyerhof W

更新日期：2013-05-31 00:00:00

abstract：:Leydig cells were purified from rat testes by discontinuous metrizamide density gradient and were shown to contain renin (EC 3.4.99.1), angiotensin-converting enzyme (dipeptidyl carboxypeptidase, (EC 3.4.15.1), and the peptide hormone angiotensins I, II and III as determined by the combined HPLC and radioimmunoassay. ...

journal_title：Biochemical and biophysical research communications

authors： Pandey KN,Misono KS,Inagami T

更新日期：1984-08-16 00:00:00

abstract：:It has been reported that changes in deoxynucleotide pool levels following exposure to UV might lead to an overestimation of the UV-induced depression in DNA synthesis as analyzed by incorporation of 3H-thymidine (1). We attempted to determine the importance of such pool effects in two ways. First, we examined the gra...

journal_title：Biochemical and biophysical research communications

authors： Griffiths TD,Ling SY

更新日期：1984-09-17 00:00:00

abstract：:Although mutations and deletions in the p53 tumor suppressor gene lead to resistance to low linear energy transfer (LET) radiation, high-LET radiation efficiently induces cell lethality and apoptosis regardless of the p53 gene status in cancer cells. Recently, it has been suggested that the induction of p53-independen...

journal_title：Biochemical and biophysical research communications

authors： Nakagawa Y,Takahashi A,Kajihara A,Yamakawa N,Imai Y,Ota I,Okamoto N,Mori E,Noda T,Furusawa Y,Kirita T,Ohnishi T

更新日期：2012-07-13 00:00:00

abstract：:The force-extension behaviour of synthesized double-stranded DNAs (dsDNAs) designed to have 2.1% or 6.6% of the thymine bases alkyne functionalized was studied using near infrared (NIR) optical tweezers. Measurements were carried out on substrates with and without flurophores covalently attached to the alkyne moiety o...

journal_title：Biochemical and biophysical research communications

authors： Raudsepp A,Kent LM,Hall SB,Williams MAK

更新日期：2018-02-12 00:00:00

abstract：:Diocleinae lectins are highly homologous in their primary structure which features metal binding sites and a carbohydrate recognition domain (CRD). Differences in the biological activity of legume lectins have been widely investigated using hemagglutination inhibition assays, isothermal titration microcalorimetry and ...

journal_title：Biochemical and biophysical research communications

authors： Bezerra EH,Rocha BA,Nagano CS,Bezerra Gde A,Moura TR,Bezerra MJ,Benevides RG,Sampaio AH,Assreuy AM,Delatorre P,Cavada BS

更新日期：2011-05-20 00:00:00

abstract：:The bioactivity, refolding, and multimer formation of endostatin, particularly of recombinant endostatin produced from bacteria, are proved challenging for clinical application. In order to determine the biological activity of recombinant endostatin multimer, first, we expressed endostatin in Escherichia coli and puri...

journal_title：Biochemical and biophysical research communications

authors： Wei DM,Gao Y,Cao XR,Zhu NC,Liang JF,Xie WP,Zhen MY,Zhu MS

更新日期：2006-07-14 00:00:00

abstract：:A human isoform of the vesicle-associated membrane protein-associated proteins (VAPs), VAPB, causes amyotrophic lateral sclerosis eight due to the missense mutation of Pro-56, whereas human VAPA and the yeast VAP Scs2p proteins are not significantly affected by similar mutations. We have found that VAPA and Scs2p have...

journal_title：Biochemical and biophysical research communications

authors： Nakamichi S,Yamanaka K,Suzuki M,Watanabe T,Kagiwada S

更新日期：2011-01-14 00:00:00

abstract：:The production of therapeutic monoclonal antibodies is costly; therefore, antigen-binding fragments (Fabs) can be used instead. However, their tendency toward aggregation can reduce the half-life in the plasma and the therapeutic effectiveness. To examine the effect of glycosylation on the properties of the Fab of a t...

journal_title：Biochemical and biophysical research communications

authors： Nakamura H,Oda-Ueda N,Ueda T,Ohkuri T

更新日期：2018-09-05 00:00:00

abstract：:To identify novel psoriasis-associated genes, we focused on several ESTs (expressed sequence tags) whose expression was predominantly increased in the affected skin in patients with psoriasis vulgaris, as assessed by microarray assay. In this paper, a full-length cDNA corresponding to one of those ESTs (AI440266) was ...

journal_title：Biochemical and biophysical research communications

authors： Matsuzaka Y,Okamoto K,Tsuji H,Mabuchi T,Ozawa A,Tamiya G,Inoko H

更新日期：2002-10-11 00:00:00

abstract：:Sea urchin spermatozoa contain a unique histone H1 (SpH1) with an unusual primary structure. Within 12 minutes postinsemination SpH1 is phosphorylated and lost from the chromatin. Both sperm and egg kinases phosphorylate SpH1 in vivo. Until now the analysis of the phosphorylation of SpH1 in eggs in vivo during the fir...

journal_title：Biochemical and biophysical research communications

authors： Porter DC,Vacquier VD

更新日期：1988-03-30 00:00:00