Thrombin-induced transfer of arachidonic acid in human platelets is not inhibited by trifluoperazine.

Abstract:

:Human platelets have been shown to contain a Ca++- and CoA-independent transacylase enzyme that catalyzes the transfer of arachidonic acid from phosphatidylcholine (PC) to lysoplasmenylethanolamine. It has been suggested that this route may represent a major source for released arachidonic acid in stimulated platelets. In this study, we have shown using arachidonic-labelled human platelets that the thrombin-induced activation of a transacylase reaction was not affected by concentrations of trifluoperazine (TFP) (15 micrograms/2 X 10(9) cells) which abolished the accumulation of free [3H]arachidonic acid in the presence of the cyclooxygenase/lipoxygenase inhibitor BW755C. TFP, at this concentration failed to block the hydrolysis of phosphatidylcholine (PC) completely and had no effect on the increased radioactivity seen in total phosphatidylethanolamine (PE) (160% of control after 4 min of incubation). These results suggest that the transacylase pathway activated in response to thrombin is not likely dependent on calcium. As TFP blocks effectively both the accumulation of free [3H]arachidonic acid and the mass of arachidonic acid without affecting the transfer of this fatty acid from PC to PE in thrombin-stimulated human platelets, it is very unlikely that the transacylation pathway represents a major source of release arachidonic acid. Based on these findings, we conclude that the above pathway may be primarily involved in the turnover of plasmenylethanolamine lipids in stimulated human platelets.

authors

Mahadevappa VG

doi

10.1016/s0006-291x(87)80038-4

subject

Has Abstract

pub_date

1987-04-29 00:00:00

pages

821-8

issue

2

eissn

0006-291X

issn

1090-2104

pii

S0006-291X(87)80038-4

journal_volume

144

pub_type

杂志文章
  • The tea flavonoid epigallocatechin-3-gallate reduces cytokine-induced VCAM-1 expression and monocyte adhesion to endothelial cells.

    abstract::Attachment of leukocytes to the vascular endothelium and the subsequent migration of cells into the vessel wall are early events in atherogenesis. This process requires the expression of endothelial adhesion molecules. Since tea catechins are reputed to promote antiatherogenic activities, we investigated the effects o...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1016/j.bbrc.2004.02.099

    authors: Ludwig A,Lorenz M,Grimbo N,Steinle F,Meiners S,Bartsch C,Stangl K,Baumann G,Stangl V

    更新日期:2004-04-09 00:00:00

  • Specific binding of phorbol esters to nuclei of human promyelocytic leukemia cells.

    abstract::In this report, we demonstrate that HL-60 nuclei isolated in calcium but not EGTA containing buffers specifically bind PE and express approximately 37,000 receptor sites/nucleus. Nuclear phorbol ester binding is lost by isolation in the absence of calcium, but can be repleted by the addition of partially purified prot...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1016/s0006-291x(87)80523-5

    authors: Kraft AS,Appling C,Berkow RL

    更新日期:1987-04-14 00:00:00

  • Differences in two mice strains on kainic acid-induced amygdalar seizures.

    abstract::Seizure susceptibility and neurodegeneration induced by microinjection of kainic acid (KA) into the left amygdala (LA) in C57BL/6 and FVB/N mouse strains were compared. Mice were administered 0.5 microg of KA into the LA. Electroencephalograms (EEGs) and behavior were monitored for 48 h after KA microinjection. Neuron...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1016/j.bbrc.2007.04.067

    authors: Kasugai M,Akaike K,Imamura S,Matsukubo H,Tojo H,Nakamura M,Tanaka S,Sano A

    更新日期:2007-06-15 00:00:00

  • Two isoforms of the rat kidney EP3 receptor derived by alternative RNA splicing: intrarenal expression co-localization.

    abstract::Two different clones, named rEP3A (approximately 2.2 kb) and rEP3B (approximately 5.2 kb), were isolated from a rat kidney cDNA library by a homology screening approach. rEP3A was shown to encode the rat kidney prostaglandin E receptor EP3 subtype (rEP3A receptor) (Takeuchi, K. et al. B.B.R.C. (1993) 194: 885). rEP3B ...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1006/bbrc.1994.1304

    authors: Takeuchi K,Takahashi N,Abe T,Abe K

    更新日期:1994-03-15 00:00:00

  • Regulation of cellular adhesion to extracellular matrix proteins by galectin-3.

    abstract::The control of cellular adhesion to extracellular matrix proteins is poorly understood. In the present analyses, we set out to test the hypothesis that high galectin-3 concentration on the cell surface downregulates cellular adhesion to the extracellular matrix proteins. Various tumor cell lines were briefly incubated...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1006/bbrc.1998.8708

    authors: Ochieng J,Leite-Browning ML,Warfield P

    更新日期:1998-05-29 00:00:00

  • Activated p53 induces NF-kappaB DNA binding but suppresses its transcriptional activation.

    abstract::NF-kappaB plays an important role in oncogenesis. Recently, we have demonstrated that loss of p53 function enhances DNA binding and transcriptional activities of NF-kappaB via IKKalpha and IKKbeta, and that glycolysis, activated by NF-kappaB, has an integral role in oncogene-induced cell transformation. Here, we show ...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1016/j.bbrc.2008.05.021

    authors: Kawauchi K,Araki K,Tobiume K,Tanaka N

    更新日期:2008-07-18 00:00:00

  • Actin cytoskeletons regulate the stretch-induced increase of Ca2+ current in human gastric myocytes.

    abstract::Using the whole-cell and single channel recording techniques, the influence of actin cytoskeletons on L-type Ca2+ current was investigated in human gastric smooth muscle cells. In isotonic condition, an actin depolymerizer cytochalasin D (Cyt-D) markedly decreased the whole-cell current (I(Ba)) without changing steady...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1016/j.bbrc.2006.11.051

    authors: Kim JH,Rhee PL,Kang TM

    更新日期:2007-01-12 00:00:00

  • Investigation of antidiabetic potential of Phyllanthus niruri L. using assays for α-glucosidase, muscle glucose transport, liver glucose production, and adipogenesis.

    abstract::Phyllanthus niruri is used in herbal medicine for treatment of diabetes. The objective of this study was to investigate the antidiabetic potential of P. niruri, using assays for α-glucosidase, muscle glucose transport, liver glucose production and adipogenesis. α-Glucosidase inhibitory activity was performed on aqueou...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1016/j.bbrc.2017.09.080

    authors: Najari Beidokhti M,Andersen MV,Eid HM,Sanchez Villavicencio ML,Staerk D,Haddad PS,Jäger AK

    更新日期:2017-11-04 00:00:00

  • On the methodological weakness of 'the effective number of codons': a reply to Marashi and Najafabadi.

    abstract::In a recent publication [Biochem. Biophys. Res. Commun. 317 (2004) 957] it was proposed that the 'effective number of codons' (Nc) in a gene should be calculated by summing the individual amino acid Nc's using rounding whenever the codon homozygosities are lower than the reciprocal value of the number of members of th...

    journal_title:Biochemical and biophysical research communications

    pub_type: 评论,杂志文章

    doi:10.1016/j.bbrc.2004.11.133

    authors: Fuglsang A

    更新日期:2005-02-04 00:00:00

  • Structures of the lamin A/C R335W and E347K mutants: implications for dilated cardiolaminopathies.

    abstract::Dilated cardiomyopathy (DCM) is a condition whereby the normal muscular function of the myocardium is altered by specific or multiple aetiologies. About 25-35% of DCM patients show familial forms of the disease, with most mutations affecting genes encoding cytoskeletal proteins. Most of the DCM-related mutations fall ...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1016/j.bbrc.2011.12.136

    authors: Bollati M,Barbiroli A,Favalli V,Arbustini E,Charron P,Bolognesi M

    更新日期:2012-02-10 00:00:00

  • Bile acids of patients with renal failure (possibility of bile acid secretion in the distal tubule).

    abstract::Bile acids of patients with renal failure and of healthy subjects were analyzed by capillary gas chromatography after group separation. The amount of bile acids in the total dialysate (150 L) of the patients was smaller than that in the 24-h urine of healthy subjects. Polar bile acid sulfates constituted 17.3% and 30....

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1016/s0006-291x(88)80699-5

    authors: Yamaga N,Adachi K,Kohara H,Shimizu K

    更新日期:1988-04-15 00:00:00

  • Transforming growth factor-beta inhibits endothelial cell proliferation.

    abstract::Transforming growth factor-beta (TGF-beta) is an inhibitor of the proliferation of bovine aortic endothelial cells in culture. Basal cell growth in serum-containing medium and cell proliferation stimulated by fibroblast growth factor (FGF) are inhibited by TGF-beta in a dose-dependent manner. Half-maximal inhibition o...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1016/0006-291x(86)91209-x

    authors: Fràter-Schröder M,Müller G,Birchmeier W,Böhlen P

    更新日期:1986-05-29 00:00:00

  • Age-related changes in the activities of antioxidant enzymes and lipid peroxidation status in ventral and dorsolateral prostate lobes of noble rats.

    abstract::In this investigation, the activities of five enzymes (catalase, superoxide dismutase, glutathione peroxidase, glutathione reductase, glucose-6-dehydrogenase) involved in oxygen radical (OR)-detoxification and antioxidative defense were measured in conjunction with an index of lipid peroxidation in major prostatic lob...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1006/bbrc.1996.0749

    authors: Ghatak S,Ho SM

    更新日期:1996-05-15 00:00:00

  • Down-regulation of Homer1b/c attenuates group I metabotropic glutamate receptors dependent Ca²⁺ signaling through regulating endoplasmic reticulum Ca²⁺ release in PC12 cells.

    abstract::The molecular basis for group I metabotropic glutamate receptors (mGluR1 and 5) coupling to membrane ion channels and intracellular calcium pools is not fully understood. Homer is a family of post synaptic density proteins functionally and physically attached to target proteins at proline-rich sequences. In the presen...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1016/j.bbrc.2014.07.044

    authors: Lv MM,Cheng YC,Xiao ZB,Sun MY,Ren PC,Sun XD

    更新日期:2014-08-08 00:00:00

  • T-kininogen--the major plasma kininogen in rat adjuvant arthritis.

    abstract::Total kininogen in plasma of Freund's adjuvant treated rats increased 20-fold 7 days following the injection. Analysis of the kininogens demonstrated that increases in T-kininogen was the major reason for the rise in kininogen. High molecular weight and low molecular weight kininogens showed little or no change. The i...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1016/0006-291x(85)91434-2

    authors: Barlas A,Okamoto H,Greenbaum LM

    更新日期:1985-05-31 00:00:00

  • The mode of chaperoning of dithiothreitol-denatured alpha-lactalbumin by alpha-crystallin.

    abstract::Molecular chaperones prevent the aggregation of partially folded or misfolded forms of protein. alpha-crystallin performs such a function in the ocular lens. To gain insight into the mechanism of the anti-aggregation activity of alpha-crystallin, we performed dynamic light scattering (DLS) measurements investigating i...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1006/bbrc.1999.1031

    authors: Bettelheim FA,Ansari R,Cheng QF,Zigler JS Jr

    更新日期:1999-08-02 00:00:00

  • Physical and functional interactions between protein tyrosine phosphatase alpha, PI 3-kinase, and PKCdelta.

    abstract::The somatostatin analogue, TT-232 inhibits cell proliferation and induces apoptosis in a variety of tumor cells both in vivo and in vitro. While the early transient activation of Erk/MAPK was found to be important for the induction of cell cycle arrest, the signaling pathway leading to the activation of Erk/MAPK had n...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1006/bbrc.2001.5811

    authors: Steták A,Csermely P,Ullrich A,Kéri G

    更新日期:2001-11-02 00:00:00

  • MicroRNA-376c suppresses non-small-cell lung cancer cell growth and invasion by targeting LRH-1-mediated Wnt signaling pathway.

    abstract::MicroRNAs (miRNAs) that negatively regulate gene expression have emerged as novel therapeutic tools for cancer treatment. In this study, we investigated the potential role of Liver receptor homolog-1 (LRH-1), a novel oncogene, in non-small-cell lung cancer (NSCLC), and examined the regulation of LRH-1 by miRNAs. We fo...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1016/j.bbrc.2016.04.002

    authors: Jiang W,Tian Y,Jiang S,Liu S,Zhao X,Tian D

    更新日期:2016-05-13 00:00:00

  • MicroRNA-215 suppresses cell proliferation, migration and invasion of colon cancer by repressing Yin-Yang 1.

    abstract::Colorectal cancer is one of the most common malignant tumors worldwide with rising incidence. MicroRNAs are small non-coding RNAs that implicate in multiple physiological or pathological processes. The aberrant expression of miRNA-215 (miR-215) has been illustrated in various types of cancers. However, the expression ...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1016/j.bbrc.2016.09.089

    authors: Chen Z,Han S,Huang W,Wu J,Liu Y,Cai S,He Y,Wu S,Song W

    更新日期:2016-10-21 00:00:00

  • GnRH neuron type-specific transcriptome analysis by laser captured single-cell microarray in the medaka.

    abstract::Most vertebrates possess at least two gonadotropin-releasing hormone (GnRH) neuron types. To understand the physiological significance of the multiple GnRH systems in the brain, we examined three GnRH neuron type-specific transcriptomes using single-cell microarray analyses in the medaka (Oryzias latipes). A microarra...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1016/j.bbrc.2013.05.004

    authors: Moriya S,Ogawa S,Parhar IS

    更新日期:2013-06-14 00:00:00

  • Induction of ornithine decarboxylase activity in mouse tissues by phorbol ester is effectively blocked by methylglyoxal bis(butylamidinohydrazone).

    abstract::Ornithine decarboxylase (ODC) was induced in the liver, lung and brain of the mouse injected intraperitoneally with 12-O-tetradecanoylphorbol 13-acetate (TPA), showing maximal enzyme activity four hours after the injection. The increase of ODC activity was due to the enhanced syntheses of mRNA and protein. The inducti...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1016/s0006-291x(87)80029-3

    authors: Hibasami H,Tsukada T,Maekawa S,Sakurai M,Nakashima K

    更新日期:1987-04-29 00:00:00

  • Enterococcus faecalis lipoteichoic acid regulates macrophages autophagy via PI3K/Akt/mTOR pathway.

    abstract::Enterococcus faecalis (E. faecalis) infection is considered an important etiological factor for the development of persistent apical periodontitis (PAP), but the exact mechanisms of autophagy between E. faecalis and immune cells remain unknown. In this study, we elucidated how E. faecalis lipoteichoic acid (LTA) is as...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1016/j.bbrc.2018.03.109

    authors: Lin D,Gao Y,Zhao L,Chen Y,An S,Peng Z

    更新日期:2018-04-15 00:00:00

  • Kinetic studies of the electron exchange reaction between the octaheme cytochrome c3 (Mr 26000) and the hydrogenase from Desulfovibrio desulfuricans Norway.

    abstract::The octaheme cytochrome c3 (Mr 26000) from Desulfovibrio desulfuricans Norway was studied using cyclic voltammetry at the pyrolytic graphite electrode. The kinetics of reduction of the octaheme cytochrome c3 (Mr 26000) from D. desulfuricans Norway by the Ni-Fe-Se hydrogenase purified from the same organism was investi...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1016/0006-291x(91)91414-8

    authors: Haladjian J,Bianco P,Guerlesquin F,Bruschi M

    更新日期:1991-08-30 00:00:00

  • Synthesis of a nonreducible cyclic analog of somatostatin having only growth hormone release inhibiting activity.

    abstract::A nonreducible cyclic analog of somatostatin (SRIF) was prepared by a combination of solid phase and solution peptide synthesis. The compound, gamma-Abu-Lys-Asn-Phe-Phe-Trp-Lys-Thr-Phe-Thr-Ser-Asp-OH, was tested for its effect on the release of growth hormone, glucagon and insulin in rats. It significantly suppressed ...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1016/0006-291x(76)90208-4

    authors: Garsky VM,Clark DE,Grant NH

    更新日期:1976-12-20 00:00:00

  • Specific binding sites for [3H]inositol(1,3,4,5)tetrakisphosphate on membranes of HL-60 cells.

    abstract::Membranes of HL-60 cells were shown to possess saturable binding sites for [3H]inositol(1,3,4,5)tetrakisphosphate, with nanomolar affinity (KD = 90 nM) and a density of 250 fmol/mg protein. The specificity of the binding sites for Ins(1,3,4,5)P4 was assessed by competition studies utilising a variety of inositol polyp...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1016/0006-291x(87)90421-9

    authors: Bradford PG,Irvine RF

    更新日期:1987-12-16 00:00:00

  • Physiological role of the association complexes of alpha-crystallin and its substrates on the chaperone activity.

    abstract::Previous reports on the chaperone activity of alpha-crystallin to prevent protein denaturation and thermal aggregation have suggested that partially denatured proteins can bind alpha-crystallin in its central region. Likewise, beta- and gamma-crystallin can also be localized to the central cavity of alpha-crystallin p...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1006/bbrc.1998.8272

    authors: Lee JS,Samejima T,Liao JH,Wu SH,Chiou SH

    更新日期:1998-03-17 00:00:00

  • Lycopene suppresses ovalbumin-induced airway inflammation in a murine model of asthma.

    abstract::In this study, we attempt to determine whether lycopene regulates inflammatory mediators in the ovalbumin (OVA)-induced murine asthma model. To address this, mice were sensitized and challenged with OVA, and then treated with lycopene before the last OVA challenge. Administration of lycopene significantly alleviated t...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1016/j.bbrc.2008.07.032

    authors: Lee CM,Chang JH,Moon DO,Choi YH,Choi IW,Park YM,Kim GY

    更新日期:2008-09-19 00:00:00

  • Inhibition of gamma-glutamyl hydrolases in human cells by 2-mercaptomethylglutaric acid.

    abstract::Cultured human lymphocytes and fibroblasts accumulate methotrexate during 24 hours and synthesize methotrexate polyglutamates up to the hexaglutamate, with the triglutamate predominating. In the interval after incubation with methotrexate, drug is lost, metabolites are converted to longer chain-lengths, and methotrexa...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1016/s0006-291x(87)80509-0

    authors: Whitehead VM,Kalman TI,Vuchich MJ

    更新日期:1987-04-14 00:00:00

  • Diet-dependent obesity and hypercholesterolemia in the New Zealand obese mouse: identification of a quantitative trait locus for elevated serum cholesterol on the distal mouse chromosome 5.

    abstract:AIMS:New Zealand obese (NZO) mice exhibit a polygenic syndrome of obesity, insulin resistance, and hypercholesterolemia that resembles the human metabolic syndrome. This study was performed in order to locate genes responsible for elevated serum cholesterol and to compare their effects under a standard and high fat die...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1016/s0006-291x(03)00664-8

    authors: Giesen K,Plum L,Kluge R,Ortlepp J,Joost HG

    更新日期:2003-05-16 00:00:00

  • Radioligand binding properties of VV-hemorphin 7, an atypical opioid peptide.

    abstract::Receptor binding properties of the hemoglobin-derived nonapeptide VV-hemorphin 7 (Val-Val-Tyr-Pro-Trp-Thr-Gln-Arg-Phe-OH) were studied using both the unlabelled form and tritium-labelled derivative of the peptide. In binding studies using selective opioid radioligands, VV-hemorphin 7 exhibited a rank order of potency ...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1006/bbrc.2001.4397

    authors: Szikra J,Benyhe S,Orosz G,Darula Z,Piot JM,Fruitier I,Monory K,Hanoune J,Borsodi A

    更新日期:2001-03-02 00:00:00