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Mouse thymoma cell line expresses a gluconeogenic enzyme, fructose 1,6-bisphosphatase.

Abstract：

:Fructose 1,6-bisphosphatase was observed in a thymic lymphoma cell line, WEH17.1 (11.5 +/- 0.8 munits/mg cytosol protein). Only a trace amount of the enzyme activity was observed in normal thymus tissue. The WEH17.1 enzyme had a pH optimum at around 7.5. The AMP-concentration giving 50% inhibition of the activity was about 73 microM. That of the crude mouse liver enzyme was 35 microM. The antibodies against the liver and intestinal enzymes cross-reacted with the WEH17.1 enzyme with a lower affinity than the liver enzyme. Immunoblot showed that the subunit molecular weight of the WEH17.1 enzyme was the same as that of the liver enzyme.

journal_name

Biochem Biophys Res Commun

journal_title

Biochemical and biophysical research communications

authors

Mizunuma H,Tashima Y

doi

10.1016/0006-291x(89)92811-8

subject

Has Abstract

pub_date

1989-02-15 00:00:00

pages

929-35

issue

3

eissn

0006-291X

issn

1090-2104

pii

0006-291X(89)92811-8

journal_volume

158

pub_type

杂志文章

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