Abstract:
:Established cancer cell lines are routinely used to study cancer. Several factors such as serial passage may affect the reproducibility of experiments with cancer cell lines, but few researches focused on these changes. In the present study, different morphology and decreased tumorigenicity were observed in late passage U87MG cells. In vitro experiments further revealed that late passage U87MG cells possessed lower invasion properties than early passage, whereas no significant differences of proliferation and migration were found between early and late passage U87MG cells. In particular, we confirmed that late passage U87MG cells exhibited more epithelial phenotype with decreased PI3K/Akt pathway and TGF-β pathway expressions at protein level. In summary, our results focused on the changes of U87MG cells during serial in vitro passage, suggested that passage-induced changes may lead to notable changes of biological characteristics and several molecular transitions in cancer cell lines, indicating the necessity to shorten experiment-span and accomplish experiments with the same or similar passage cancer cell strains.
journal_name
Cell Mol Neurobioljournal_title
Cellular and molecular neurobiologyauthors
Zeng Y,Wang X,Wang J,Yi R,Long H,Zhou M,Luo Q,Zhai Z,Song Y,Qi Sdoi
10.1007/s10571-018-0592-7subject
Has Abstractpub_date
2018-08-01 00:00:00pages
1245-1252issue
6eissn
0272-4340issn
1573-6830pii
10.1007/s10571-018-0592-7journal_volume
38pub_type
杂志文章abstract::The transforming growth factor betas (TGFbetas) are context-dependent regulators of neurons in vitro, but their physiological functions in the brain are unclear. Haploinsufficiency of either Tgfbeta1 or Tgfbeta2 leads to age-related deterioration of neurons, but the development of the brain is normal in the full absen...
journal_title:Cellular and molecular neurobiology
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doi:10.1007/s10571-009-9354-x
更新日期:2009-07-01 00:00:00
abstract::Elevated iron levels in the substantia nigra (SN) participate in neuronal death in Parkinson's disease (PD), while the mechanisms underlying the increased iron are still unknown. Ceruloplasmin (CP), a ferroxidase, converts highly toxic ferrous iron to its non-toxic ferric form, which cooperated with ferroportin1 (FP1)...
journal_title:Cellular and molecular neurobiology
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更新日期:2015-07-01 00:00:00
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journal_title:Cellular and molecular neurobiology
pub_type: 杂志文章
doi:10.1007/s10571-010-9518-8
更新日期:2010-08-01 00:00:00
abstract::1. Data obtained studying permeability characteristics of single Deiters' membranes in a microchamber system show that intracellular GABA can activate chloride in-->out passage with a GABAA pharmacology. 2. The overall data suggest the presence of a chloride extrusion pump in these neurons based on intracellular GABA ...
journal_title:Cellular and molecular neurobiology
pub_type: 杂志文章
doi:10.1023/a:1006944820946
更新日期:1999-12-01 00:00:00
abstract::Methylmalonic acidemia and propionic acidemia are organic acidemias biochemically characterized by predominant tissue accumulation of methylmalonic acid (MMA) and propionic acid (PA), respectively. Affected patients present predominantly neurological symptoms, whose pathogenesis is not yet fully established. In the pr...
journal_title:Cellular and molecular neurobiology
pub_type: 杂志文章
doi:10.1007/s10571-011-9675-4
更新日期:2011-07-01 00:00:00
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journal_title:Cellular and molecular neurobiology
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doi:10.1007/BF00733792
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journal_title:Cellular and molecular neurobiology
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更新日期:2010-05-01 00:00:00
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journal_title:Cellular and molecular neurobiology
pub_type: 杂志文章
doi:10.1007/BF02152058
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journal_title:Cellular and molecular neurobiology
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doi:10.1007/s10571-017-0483-3
更新日期:2017-11-01 00:00:00
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journal_title:Cellular and molecular neurobiology
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更新日期:2020-07-01 00:00:00
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journal_title:Cellular and molecular neurobiology
pub_type: 杂志文章
doi:10.1007/s10571-015-0172-z
更新日期:2015-08-01 00:00:00
abstract:AIMS:Treatments that increase acetylcholine release from brain slices decrease the synthesis of phosphatidylcholine by, and its levels in, the slices. We examined whether adding cytidine or uridine to the slice medium, which increases the utilization of choline to form phospholipids, also decreases acetylcholine levels...
journal_title:Cellular and molecular neurobiology
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doi:10.1007/s10571-006-9004-5
更新日期:2006-07-01 00:00:00
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journal_title:Cellular and molecular neurobiology
pub_type: 杂志文章
doi:10.1007/s10571-020-00836-z
更新日期:2021-01-01 00:00:00
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journal_title:Cellular and molecular neurobiology
pub_type: 杂志文章
doi:10.1007/BF00711228
更新日期:1988-12-01 00:00:00
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journal_title:Cellular and molecular neurobiology
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doi:10.1007/s10571-017-0566-1
更新日期:2018-05-01 00:00:00
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journal_title:Cellular and molecular neurobiology
pub_type: 杂志文章
doi:10.1007/BF02071882
更新日期:1995-08-01 00:00:00
abstract::Amyotrophic lateral sclerosis (ALS) is an adult-onset, progressive, and fatal neurodegenerative disease caused by selective loss of motor neurons. Both ALS model mice and patients with sporadic ALS have increased levels of prostaglandin E2 (PGE2). Furthermore, the protein levels of microsomal PGE synthase-1 and cycloo...
journal_title:Cellular and molecular neurobiology
pub_type: 杂志文章
doi:10.1007/s10571-016-0377-9
更新日期:2017-04-01 00:00:00
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journal_title:Cellular and molecular neurobiology
pub_type: 杂志文章
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journal_title:Cellular and molecular neurobiology
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更新日期:2005-06-01 00:00:00
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journal_title:Cellular and molecular neurobiology
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更新日期:2017-04-01 00:00:00
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journal_title:Cellular and molecular neurobiology
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更新日期:1996-04-01 00:00:00
abstract::1. The proteins of large dense-core vesicles (LDV) in neuroendocrine tissues are well characterized. Secretory components comprise chromogranins and neuropeptides. Intrinsic membrane proteins include cytochrome b-561, transporters, SV2, synaptotagmin, and synaptobrevin. 2. The effects of stimulation and of second mess...
journal_title:Cellular and molecular neurobiology
pub_type: 杂志文章,评审
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更新日期:1998-04-01 00:00:00
abstract::Endothelins regulate cellular functions in the mammalian brain through the endothelin receptors A and B (EDNRA and EDNRB). In this study, we investigated the role of EDNRB on cell proliferation in the cerebellum by using the spotting lethal (sl) rat, which carries a naturally occurring deletion in the EDNRB gene. Prol...
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更新日期:2008-12-01 00:00:00
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journal_title:Cellular and molecular neurobiology
pub_type: 杂志文章
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更新日期:2018-10-01 00:00:00
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journal_title:Cellular and molecular neurobiology
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更新日期:2013-03-01 00:00:00
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journal_title:Cellular and molecular neurobiology
pub_type: 杂志文章
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journal_title:Cellular and molecular neurobiology
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更新日期:2011-07-01 00:00:00
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journal_title:Cellular and molecular neurobiology
pub_type: 杂志文章
doi:10.1023/a:1006972709733
更新日期:1999-02-01 00:00:00
abstract::1. Long before onset of synaptogenesis in the chicken neural tube, the closely related enzymes butyrylcholinesterase (BChE) and acetylcholinesterase (AChE) are expressed in a mutually exclusive manner. Accordingly, neuroblasts on the ventricular side of the neural tube transiently express BChE before they abruptly acc...
journal_title:Cellular and molecular neurobiology
pub_type: 杂志文章,评审
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更新日期:1991-02-01 00:00:00