Abstract:
:1. Antidepressants have been used clinically for many years; however, the neurochemical mechanism for their therapeutic effect has not been clarified yet. Recent reports indicate that chronic antidepressant treatment directly affects the postsynaptic membrane to increase the coupling between the stimulatory GTP-binding (G) protein, Gs, and adenylyl cyclase. Tubulin, a cytoskeletal element, is involved in the stimulatory and inhibitory regulation of adenylyl cyclase in rat cerebral cortex via direct transfer of GTP to G proteins. In this study, we investigated whether the functional change of the adenylyl cyclase system caused by chronic antidepressant treatment involves an alteration of tubulin function in the regulation of adenylyl cyclase activity. 2. Male Sprague-Dawley rats were treated once daily with amitriptyline or saline by intraperitoneal injection (10 mg/kg) for 21 days, and their cerebral cortex membranes and GppNHp-liganded tubulin (tubulin-GppNHp) were prepared for what. 3. GppNHp-stimulated adenylyl cyclase activity in cortex membranes from amitriptyline-treated rats was significantly higher than that in control membranes. Furthermore, tubulin-GppNHp prepared from amitriptyline-treated rats was more potent than that from control rats in the stimulation of adenylyl cyclase activity in the cortex membranes of the controls. However, there was no significant difference in manganese-stimulated adenylyl cyclase activity between control and amitriptyline-treated rats. 4. The present results suggest that chronic antidepressant treatment enhances not only the coupling between Gs and the catalytic subunit of adenylyl cyclase but also tubulin interaction with Gs in the cerebral cortex of the rat.
journal_name
Cell Mol Neurobioljournal_title
Cellular and molecular neurobiologyauthors
Kamada H,Saito T,Hatta S,Toki S,Ozawa H,Watanabe M,Takahata Ndoi
10.1023/a:1006972709733subject
Has Abstractpub_date
1999-02-01 00:00:00pages
109-17issue
1eissn
0272-4340issn
1573-6830journal_volume
19pub_type
杂志文章abstract::Abeta accumulation, a hallmark of Alzheimer's disease, promotes the disease progress in multiple facets. Abeta is formed through amyloidogenic cleavage pathway of amyloid precursor protein (APP). Production of Abeta can be decreased via activation of 5-HT2C receptor, which enhances alternative APP non-amyloidogenic cl...
journal_title:Cellular and molecular neurobiology
pub_type: 杂志文章
doi:10.1007/s10571-014-0138-6
更新日期:2015-04-01 00:00:00
abstract::1. A lambda phage genomic DNA library for Limulus (L.) polyphemus brain was constructed using the AGEM-12 vector and the host strain KW251. 2. The primary library contained approximately 1.275 x 10(6) independent clones, increasing upon amplfication to 6.66 x 10(9) pfu/ml in a total volume of 58 ml. 3. A total of 28 c...
journal_title:Cellular and molecular neurobiology
pub_type: 杂志文章
doi:10.1023/a:1007027232412
更新日期:2000-08-01 00:00:00
abstract::Both sex and steroid hormones are important to consider in human ischemic stroke and its experimental models. Stroke initiates a cascade of changes that lead to neural cell death, but also activates endogenous protective processes that counter the deleterious consequences of ischemia. Steroids may be part of these cer...
journal_title:Cellular and molecular neurobiology
pub_type: 杂志文章,评审
doi:10.1007/s10571-018-0627-0
更新日期:2019-05-01 00:00:00
abstract::EP3 is prostaglandin E2 receptor subtype 3 and mediates the activation of several signaling pathways, changing in cAMP levels, calcium mobilization, and activation of phospholipase C. Previous studies demonstrated a direct role for EP3 in various neurodegenerative disorders, such as stroke and Alzheimer disease. Howev...
journal_title:Cellular and molecular neurobiology
pub_type: 杂志文章
doi:10.1007/s10571-015-0287-2
更新日期:2016-08-01 00:00:00
abstract::Acute encephalopathy is a generic term for acute brain dysfunction occurring after infection. Acute encephalopathy induced by influenza virus results in high mortality, and most cases of influenza-associated encephalopathy (IAE) result in brain edema. Administration of diclofenac sodium (DCF), a non-steroidal anti-inf...
journal_title:Cellular and molecular neurobiology
pub_type: 杂志文章
doi:10.1007/s10571-013-9905-z
更新日期:2013-04-01 00:00:00
abstract::The Pax5, a B-cell-Specific Activator Protein (BSAP) and redox-sensitive transcription factor, is expressed in the immune-privileged brain, B-lymphocytes, lymph nodes and spleen. PAX5-mediated immune pathway has also been described in the progression of Glioblastoma multiforme. However, the status of Pax5 and its role...
journal_title:Cellular and molecular neurobiology
pub_type: 杂志文章
doi:10.1007/s10571-017-0566-1
更新日期:2018-05-01 00:00:00
abstract::In order to guarantee better conditions for competition, the nervous system has developed not only mechanisms controlling muscle effectors, but also retrograde systems that, starting from peripheral structures, may influence brain functions. Under such perspective, physical activity could play an important role in inf...
journal_title:Cellular and molecular neurobiology
pub_type: 杂志文章,评审
doi:10.1007/s10571-009-9488-x
更新日期:2010-05-01 00:00:00
abstract::Working memory (WM) is a highly heritable cognitive trait that is involved in many higher-level cognitive functions. In the past few years, much evidence has indicated that the reduction of dopamine activity in human brain can impair the WM system of the neuropsychiatric disorders. In this study, we hypothesized that ...
journal_title:Cellular and molecular neurobiology
pub_type: 杂志文章
doi:10.1007/s10571-012-9817-3
更新日期:2012-08-01 00:00:00
abstract::Consisting of a fragment of ACTH(4-7) and C-terminal PGP tripeptide, the polypeptide Semax is successfully used for acute stroke therapy. Previous experiments showed rapid induction of Bdnf, Ngf, and TrkB expression in intact rat hippocampus following Semax treatment. To investigate the mRNA expression of neurotrophin...
journal_title:Cellular and molecular neurobiology
pub_type: 杂志文章
doi:10.1007/s10571-009-9432-0
更新日期:2010-01-01 00:00:00
abstract::1. Coexisting with oxytocin or vasopressin in the cell bodies and nerve terminals of the hypothalamic-neurohypophysial system are smaller amounts of other peptides. For a number of these "copeptides" there is strong evidence of corelease with the major magnocellular hormones. Guided by the location of their specific r...
journal_title:Cellular and molecular neurobiology
pub_type: 杂志文章,评审
doi:10.1007/BF00712791
更新日期:1989-12-01 00:00:00
abstract::1. Compared to pineal N-acetyl transferase (NAT) activity, which exhibited a dramatic drop following acute light exposure at night, nocturnal rat pineal thyroxine type II 5'-deiodinase (5'-D) activity was minimally influenced by the same light exposure. The injection of cycloheximide, a potent inhibitor of protein syn...
journal_title:Cellular and molecular neurobiology
pub_type: 杂志文章
doi:10.1007/BF00711228
更新日期:1988-12-01 00:00:00
abstract::1. Alzheimer's disease is characterized by the deposition in the brain of extracellular amyloid plaques and vascular deposits consisting mostly of amyloid beta-peptide (A beta). A beta, a polypeptide of 39-43 amino acids (M(r), approximately 4 kDa), is derived proteolytically from a family of proteins of 695-770 amino...
journal_title:Cellular and molecular neurobiology
pub_type: 杂志文章
doi:10.1007/BF02088713
更新日期:1994-08-01 00:00:00
abstract::The study was performed to investigate the effect of combination therapy with aminoguanidine (AG) and dexamethasone (DEX) on the compression spinal cord injury (SCI) in rat. Compared to the control group, the combination therapy group with AG (75 mg/kg) and DEX (0.025 mg/kg) significantly reduced the degree of (1) spi...
journal_title:Cellular and molecular neurobiology
pub_type: 杂志文章
doi:10.1007/s10571-009-9380-8
更新日期:2009-07-01 00:00:00
abstract::1. The term "blood-brain barrier" describes a range of mechanisms that control the exchange of molecules between the internal environment of the brain and the rest of the body. 2. The underlying morphological feature of these barriers is the presence of tight junctions which are present between cerebral endothelial ce...
journal_title:Cellular and molecular neurobiology
pub_type: 杂志文章,评审
doi:10.1023/a:1006991809927
更新日期:2000-02-01 00:00:00
abstract::It is now well accepted that inflammatory responses in brain contribute to the genesis and evolution of damage in neurological diseases, trauma, and infection. Inflammatory mediators including cytokines, cell adhesion molecules, and reactive oxygen species including NO are detected in human brain and its animal models...
journal_title:Cellular and molecular neurobiology
pub_type: 杂志文章,评审
doi:10.1023/a:1025084415833
更新日期:2003-10-01 00:00:00
abstract::In rats, variations in the levels of neuromodulatory molecules and in the expression of their receptors are observed during pregnancy and postpartum. These changes may contribute to the development and management of maternal behavior. The frequency of licking the pups is used to evaluate maternal care, having mothers ...
journal_title:Cellular and molecular neurobiology
pub_type: 杂志文章
doi:10.1007/s10571-015-0306-3
更新日期:2016-10-01 00:00:00
abstract::1. The aim of the present study is to map the incipient phase of Fos expression in the sacral spinal cord neuronal pools of multiple cauda equina constrictions canine model. 2. Fos-positive neurons were found bilaterally in the lateral portion of superficial dorsal horn layers (Laminae I-III) and along the lateral edg...
journal_title:Cellular and molecular neurobiology
pub_type: 杂志文章
doi:10.1023/a:1012610407025
更新日期:2001-08-01 00:00:00
abstract::The effects of the calcium channel blocker D-600 on the cation channels activated by acetylcholine (ACh) was studied in voltage-clamped Aplysia neurons by voltage-jump relaxation analysis. D-600 blocked the steady-state ACh current in a highly voltage-dependent manner, the degree of antagonism increasing with membrane...
journal_title:Cellular and molecular neurobiology
pub_type: 杂志文章
doi:10.1007/BF00734714
更新日期:1983-12-01 00:00:00
abstract::Hippocampal structures are a major target for adrenal steroid hormones, and hence these neural regions are some of the most likely mediators of the effects of adrenocortical steroids on behavior. Memory disturbance, in particular biasing toward negative contents, are part of the symptomatology presented by depressive ...
journal_title:Cellular and molecular neurobiology
pub_type: 杂志文章
doi:10.1007/BF00711580
更新日期:1993-08-01 00:00:00
abstract::Microelectrophoretic application of sex hormones onto pineal cells in guinea pigs has shown different responses in pregnant females as compared to males. In pregnant females estrone caused excitation in 74% of the cells tested, while progesterone and testosterone, prolactin, and HCG were inhibitory in a majority of th...
journal_title:Cellular and molecular neurobiology
pub_type: 杂志文章
doi:10.1007/BF00710681
更新日期:1981-09-01 00:00:00
abstract::One of the few areas of the adult CNS, that are known to be competent for neuronal proliferation, is the subventricular zone (SVZ) lining the brain lateral ventricles. Cells proliferating in the SVZ migrate along a defined pathway, the rostral migratory stream (RMS), where their proliferation continues until reaching ...
journal_title:Cellular and molecular neurobiology
pub_type: 杂志文章
doi:10.1007/s10571-006-9067-3
更新日期:2006-10-01 00:00:00
abstract::Pain is defined as an unpleasant sensory and emotional experience associated with actual or potential tissue damage. The opioid epidemic in the USA has highlighted the need for alternative treatments for pain. Following reports on the opioid interactions of various antipsychotic medications, we speculated that the inv...
journal_title:Cellular and molecular neurobiology
pub_type: 杂志文章
doi:10.1007/s10571-020-01001-2
更新日期:2020-11-13 00:00:00
abstract::Established cancer cell lines are routinely used to study cancer. Several factors such as serial passage may affect the reproducibility of experiments with cancer cell lines, but few researches focused on these changes. In the present study, different morphology and decreased tumorigenicity were observed in late passa...
journal_title:Cellular and molecular neurobiology
pub_type: 杂志文章
doi:10.1007/s10571-018-0592-7
更新日期:2018-08-01 00:00:00
abstract::1. The effect of transient forebrain ischemia on endothelin-1 (ET-1) and endothelin-3 (ET-3) production in the hippocampus of stroke-prone spontaneously hypertensive rats (SHRSPs) was investigated using immunohistochemical techniques. 2. In SHRSPs subjected to 10-min bilateral carotid occlusion, neuronal degeneration ...
journal_title:Cellular and molecular neurobiology
pub_type: 杂志文章
doi:10.1007/BF00712986
更新日期:1993-02-01 00:00:00
abstract::We previously found that xanthotoxol, one of the major active ingredients in Cnidium monnieri (L.) Cusson, exerts protective effects in a rat model of focal cerebral ischemia/reperfusion injury by alleviating brain edema, inhibiting the neutrophil infiltration, and decreasing the expression of intercellular adhesion m...
journal_title:Cellular and molecular neurobiology
pub_type: 杂志文章
doi:10.1007/s10571-013-9939-2
更新日期:2013-07-01 00:00:00
abstract::Ubiquitination of proteins and their degradation within the proteasome has emerged as the major proteolytic mechanism used by mammalian cells to regulate cytosolic and nuclear protein levels. Substrate ubiquitylation is mediated by ubiquitin (Ub) ligases, also called E3 Ub ligases. HECT-E3 Ub ligases are characterized...
journal_title:Cellular and molecular neurobiology
pub_type: 杂志文章
doi:10.1007/s10571-008-9303-0
更新日期:2009-02-01 00:00:00
abstract::Human mesenchymal stem cells (MSCs) are considered a promising tool for cell-based therapies of nervous system diseases. Bone marrow (BM) has been the traditional source of MSCs (BM-MSCs). However, there are some limitations for their clinical use, such as the decline in cell number and differentiation potential with ...
journal_title:Cellular and molecular neurobiology
pub_type: 杂志文章
doi:10.1007/s10571-013-9922-y
更新日期:2013-05-01 00:00:00
abstract::In our previous research, the formation and development of age-related cataract (ARC) is associated with DNA hypermethylation of some genes in lens epithelial cells (LECs). This study aimed to investigate the expression profile of DNA methylation- and transcriptional repression-associated genes in LECs of ARC. The exp...
journal_title:Cellular and molecular neurobiology
pub_type: 杂志文章
doi:10.1007/s10571-016-0393-9
更新日期:2017-04-01 00:00:00
abstract::Traumatic brain injury (TBI) causes permanent neurological and cognitive impairments. Effective pharmacological interventions remain elusive. Spermidine is a polyamine compound found in our body that may play a role in brain development and congenital function. In this study, we aimed to investigate the therapeutic po...
journal_title:Cellular and molecular neurobiology
pub_type: 杂志文章
doi:10.1007/s10571-019-00783-4
更新日期:2020-08-01 00:00:00
abstract::The stimulation of peripheral opioid receptors counteracts thermal hyperalgesia produced by the intratibial inoculation of NCTC 2472 cells in mice, through the activation of the nitric oxide/cGMP/ATP-sensitive K+-channels (NO/cGMP/K(+) (ATP)) cascade (Menéndez et al. 2007, Neuropharmacology 53:71-80). We aimed to eluc...
journal_title:Cellular and molecular neurobiology
pub_type: 杂志文章
doi:10.1007/s10571-008-9272-3
更新日期:2008-11-01 00:00:00