Abstract:
:The Pax5, a B-cell-Specific Activator Protein (BSAP) and redox-sensitive transcription factor, is expressed in the immune-privileged brain, B-lymphocytes, lymph nodes and spleen. PAX5-mediated immune pathway has also been described in the progression of Glioblastoma multiforme. However, the status of Pax5 and its role in brain immunity are not yet elucidated. In silico analysis of Pax5 interacting proteins predicts its interaction with proteins of cell proliferation, differentiation of hematopoietic cells, neurogenesis and several cell signalling pathways. Promoter analysis shows multiple binding sites for Pax5 in promoter of ionized calcium-binding adapter molecule 1 (Iba1). Like Iba1, Pax5 is also associated with inflammatory and immune response, activation of leukocyte and remodelling of actin cytoskeleton. Therefore, localization and interaction of Pax5 with Iba1 in brain of mice were studied using Chromatin Immunoprecipitation (ChIP), Co-Immunoprecipitation (Co-IP) and Immuno-fluorescence assay. The Pax5- and Iba1-positive cells were observed in cerebral cortex, cerebellum, olfactory bulb, hippocampus, and ventricles of brain. The co-localization of Pax5 and Iba1 was evident in microglia in almost all evaluated regions of brain. In some regions, Pax5- and Iba1-positive were distinctly compartmentalized. The Pax5a/b interacts with Iba1 and binds to its regulatory sequences. Results indicate Pax5-associated activities of Iba1 in microglia in brain of mice.
journal_name
Cell Mol Neurobioljournal_title
Cellular and molecular neurobiologyauthors
Maurya SK,Mishra Rdoi
10.1007/s10571-017-0566-1subject
Has Abstractpub_date
2018-05-01 00:00:00pages
919-927issue
4eissn
0272-4340issn
1573-6830pii
10.1007/s10571-017-0566-1journal_volume
38pub_type
杂志文章abstract::1. Human medulloblastoma (ONS-76), a central nervous system (CNS)-derived undifferentiated cell line, was found to possess glial characteristics as defined by responses in the interferon (IFN) system; ONS-76 cells produced as much IFN-beta as human fibroblast and glioma cells by viral infection and poly(I):poly(C) ind...
journal_title:Cellular and molecular neurobiology
pub_type: 杂志文章
doi:10.1023/a:1026327309345
更新日期:1998-10-01 00:00:00
abstract::1. The possibility that a long-lasting neuronal activation regulates the expression of muscarinic cholinergic receptors was studied with three cultured neuronal cell lines. 2. Continuous depolarization of a subclone of the neuroblastoma-glioma NG108-15 hybrid cells with potassium chloride increased by 45-75% the numbe...
journal_title:Cellular and molecular neurobiology
pub_type: 杂志文章
doi:10.1007/BF00711445
更新日期:1989-03-01 00:00:00
abstract::1. Compared to pineal N-acetyl transferase (NAT) activity, which exhibited a dramatic drop following acute light exposure at night, nocturnal rat pineal thyroxine type II 5'-deiodinase (5'-D) activity was minimally influenced by the same light exposure. The injection of cycloheximide, a potent inhibitor of protein syn...
journal_title:Cellular and molecular neurobiology
pub_type: 杂志文章
doi:10.1007/BF00711228
更新日期:1988-12-01 00:00:00
abstract::Calmodulin is known to transduce Ca(2+) signals by interacting with specific target proteins. In order to determine the role of calmodulin in regulating neuronal survival and death, we examined, whether calmodulin inhibitors induce caspase-dependent apoptotic cell death, and whether glycogen synthase kinase-3 is invol...
journal_title:Cellular and molecular neurobiology
pub_type: 杂志文章
doi:10.1007/s10571-007-9172-y
更新日期:2007-09-01 00:00:00
abstract::Cannabinoid (CB) ligands have demonstrated neuroprotective properties. In this study we compared the effects of a diverse set of CB ligands against β amyloid-mediated neuronal toxicity and activated microglial-conditioned media-based neurotoxicity in vitro, and compared this with a capacity to directly alter β amyloid...
journal_title:Cellular and molecular neurobiology
pub_type: 杂志文章
doi:10.1007/s10571-013-9984-x
更新日期:2014-01-01 00:00:00
abstract::The intrauterinal development in mammals represents a very sensitive period of life in relation to many environmental factors, including ionizing radiation (IR). The developing nervous system is particularly vulnerable to IR, and the consequences of exposure are of importance because of its potential health risks. The...
journal_title:Cellular and molecular neurobiology
pub_type: 杂志文章
doi:10.1007/s10571-014-0144-8
更新日期:2015-01-01 00:00:00
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journal_title:Cellular and molecular neurobiology
pub_type: 杂志文章
doi:10.1007/s10571-017-0483-3
更新日期:2017-11-01 00:00:00
abstract::The NMDA receptor is the most widely studied ionotropic glutamate receptor, and it is central to many physiological and pathophysiological processes in the central nervous system. GluN2A is one of the two main types of GluN2 NMDA receptor subunits in the forebrain. The proper activity of GluN2A is important to brain f...
journal_title:Cellular and molecular neurobiology
pub_type: 杂志文章,评审
doi:10.1007/s10571-016-0388-6
更新日期:2017-04-01 00:00:00
abstract::We previously found that xanthotoxol, one of the major active ingredients in Cnidium monnieri (L.) Cusson, exerts protective effects in a rat model of focal cerebral ischemia/reperfusion injury by alleviating brain edema, inhibiting the neutrophil infiltration, and decreasing the expression of intercellular adhesion m...
journal_title:Cellular and molecular neurobiology
pub_type: 杂志文章
doi:10.1007/s10571-013-9939-2
更新日期:2013-07-01 00:00:00
abstract::Ischemic preconditioning (IPC) represents an important adaptation of CNS to sub-lethal ischemia, which results in increased tolerance of CNS to the lethal ischemia. Ischemia-induced mitochondrial apoptosis is considered to be an important event leading to neuronal cell death after cerebral blood flow arrest. In presen...
journal_title:Cellular and molecular neurobiology
pub_type: 杂志文章
doi:10.1007/s10571-009-9373-7
更新日期:2009-09-01 00:00:00
abstract::1. In the retinal inner nuclear layer of the majority of species, a dopaminergic neuronal network has been visualized in either amacrine cells or the so-called interplexiform cells. 2. Binding studies of retinal dopamine receptors have revealed the existence of both D1- as well D2-subtypes. The D1-subtype was characte...
journal_title:Cellular and molecular neurobiology
pub_type: 杂志文章,评审
doi:10.1007/BF00711177
更新日期:1990-09-01 00:00:00
abstract::Ubiquitination of proteins and their degradation within the proteasome has emerged as the major proteolytic mechanism used by mammalian cells to regulate cytosolic and nuclear protein levels. Substrate ubiquitylation is mediated by ubiquitin (Ub) ligases, also called E3 Ub ligases. HECT-E3 Ub ligases are characterized...
journal_title:Cellular and molecular neurobiology
pub_type: 杂志文章
doi:10.1007/s10571-008-9303-0
更新日期:2009-02-01 00:00:00
abstract::1. We have devised a radioimmunoassay for atrial natriueretic factor (ANF). Its application to rat brain extract led to the discovery of ANF in the brain. In addition to the hypothalamus and the pontine medullary region, it was widely distributed. 2. ANF in the brain is stored in a low molecular weight form, in contra...
journal_title:Cellular and molecular neurobiology
pub_type: 杂志文章,评审
doi:10.1007/BF00711444
更新日期:1989-03-01 00:00:00
abstract::1. Data obtained studying permeability characteristics of single Deiters' membranes in a microchamber system show that intracellular GABA can activate chloride in-->out passage with a GABAA pharmacology. 2. The overall data suggest the presence of a chloride extrusion pump in these neurons based on intracellular GABA ...
journal_title:Cellular and molecular neurobiology
pub_type: 杂志文章
doi:10.1023/a:1006944820946
更新日期:1999-12-01 00:00:00
abstract::1. The original concept of the ischemic penumbra surrounding a focus of dense cerebral ischemia is based on electrophysiological observations. In the cortex of baboons following middle cerebral artery occlusion, complete failure of the cortical evoked potential was observed at a cerebral blood flow (CBF) threshold lev...
journal_title:Cellular and molecular neurobiology
pub_type: 杂志文章,评审
doi:10.1023/a:1020629818207
更新日期:1998-12-01 00:00:00
abstract::1. Photoreceptor terminals in the flies Musca domestica and Drosophila melanogaster have been reconstructed in three dimensions from serial EM to reveal the surface distributions of afferent tetrad synapses. 2. The terminals are cylindrical and surround two target cells; they have synaptic sites distributed along thei...
journal_title:Cellular and molecular neurobiology
pub_type: 杂志文章
doi:10.1007/BF02151904
更新日期:1996-12-01 00:00:00
abstract::Intrachain disulfide bond formation among the cysteine thiols of SNAP-25, a component of the SNARE protein complex required for neurotransmitter release, has been hypothesized to link oxidative stress and inhibition of synaptic transmission. However, neither the availability in vivo of SNAP-25 thiols, which are known ...
journal_title:Cellular and molecular neurobiology
pub_type: 杂志文章
doi:10.1007/s10571-011-9748-4
更新日期:2012-03-01 00:00:00
abstract::Alzheimer's disease (AD) is characterized by deposition of beta-amyloid peptides (Aβ) and progressive loss of neurons. Neural stem/progenitor cells (NSPCs) can proliferate and produce immature neurons even in the brain of AD patients. However, Aβ42 significantly decreased the expression of RhoC in NSPCs during the co-...
journal_title:Cellular and molecular neurobiology
pub_type: 杂志文章
doi:10.1007/s10571-014-0026-0
更新日期:2014-04-01 00:00:00
abstract::Membrane responses to cyclic adenosine monophosphate (cAMP) injections have been studied by means of voltage clamp, Ca-indicator dye, and ion substitution techniques in identified neurons from the abdominal ganglion of Limax maximus. The ventral abdominal giant cell (AGC) displayed a response consisting of a decrease ...
journal_title:Cellular and molecular neurobiology
pub_type: 杂志文章
doi:10.1007/BF00733595
更新日期:1984-12-01 00:00:00
abstract::1. We have used horseradish peroxidase-conjugated protein A- and 125I-protein A to develop immunohistochemical and radioimmunohistochemical methods for the localization of antigens in brain and other tissues of the rat. 2. We visualized methionine-enkephalin fibers in the rat brain by incubating tissue sections with a...
journal_title:Cellular and molecular neurobiology
pub_type: 杂志文章
doi:10.1007/BF00712912
更新日期:1988-03-01 00:00:00
abstract::Hydrogen peroxide (H(2)O(2)), a major reactive oxygen species produced during oxidative stress, has been implicated in the pathophysiology of various neurodegenerative conditions. Cyperus rotundus is a traditional medicinal herb that has recently found applications in food and confectionary industries. In the current ...
journal_title:Cellular and molecular neurobiology
pub_type: 杂志文章
doi:10.1007/s10571-012-9865-8
更新日期:2013-01-01 00:00:00
abstract::Dideoxycytidine (zalcitabine, ddC) produces neurotoxic effects. It is particularly important to understand the toxic effects of ddC on different subpopulations of dorsal root ganglion (DRG) neurons which express distinct tyrosine kinase receptor (Trk) and to find therapeutic factors for prevention and therapy for ddC-...
journal_title:Cellular and molecular neurobiology
pub_type: 杂志文章
doi:10.1007/s10571-013-0001-1
更新日期:2014-03-01 00:00:00
abstract::It is now well accepted that inflammatory responses in brain contribute to the genesis and evolution of damage in neurological diseases, trauma, and infection. Inflammatory mediators including cytokines, cell adhesion molecules, and reactive oxygen species including NO are detected in human brain and its animal models...
journal_title:Cellular and molecular neurobiology
pub_type: 杂志文章,评审
doi:10.1023/a:1025084415833
更新日期:2003-10-01 00:00:00
abstract::Normokalemic periodic paralysis (normoPP) is a type of skeletal muscle function disorder which is characterized by paralysis attack with concomitant normal serum potassium level. We previously reported that R675Q mutation of human skeletal muscle voltage-gated sodium channel α subunit (SCN4A) may be the novel mutation...
journal_title:Cellular and molecular neurobiology
pub_type: 杂志文章
doi:10.1007/s10571-014-0052-y
更新日期:2014-07-01 00:00:00
abstract::1. The term "blood-brain barrier" describes a range of mechanisms that control the exchange of molecules between the internal environment of the brain and the rest of the body. 2. The underlying morphological feature of these barriers is the presence of tight junctions which are present between cerebral endothelial ce...
journal_title:Cellular and molecular neurobiology
pub_type: 杂志文章,评审
doi:10.1023/a:1006991809927
更新日期:2000-02-01 00:00:00
abstract::Oxidative stress is one of the mechanisms implicated to play a significant role in the pathophysiology of Parkinson's disease. Previously, we showed that an aqueous extract of Selaginella delicatula (SDAE) offered robust neuroprotection against rotenone (ROT) in a Drosophila model. In furtherance in the present study,...
journal_title:Cellular and molecular neurobiology
pub_type: 杂志文章
doi:10.1007/s10571-013-9959-y
更新日期:2013-10-01 00:00:00
abstract::1. We describe here the alterations in the nociceptive sensitivity of Swiss CD1 mice receiving an intraplantar (i.pl.) administration of XC Rous sarcoma-virus-transformed rat fibroblasts (XC cells). 2. Histological studies reveal that XC cells remain at the injection site 2-3 weeks after implantation, a time at which ...
journal_title:Cellular and molecular neurobiology
pub_type: 杂志文章
doi:10.1023/b:cemn.0000018621.58328.ea
更新日期:2004-04-01 00:00:00
abstract::1. The visual transduction system of the vertebrate retina is a well-studied model for biochemical and molecular studies of signal transduction. The structure and function of rhodopsin, a prototypical G protein-coupled receptor, and transducin or Gt, the photoreceptor G protein, have been particularly well studied. Me...
journal_title:Cellular and molecular neurobiology
pub_type: 杂志文章,评审
doi:10.1007/BF00741446
更新日期:1991-12-01 00:00:00
abstract::Thyroid hormone (T(3)) plays an essential role in the central nervous system development. Astrocytes mediate many of the T(3) effects in the growth and differentiation of cerebellum. In culture, T(3) induces cerebellar astrocytes to secrete growth factors, mainly FGF(2), and alters the expression and organization of t...
journal_title:Cellular and molecular neurobiology
pub_type: 杂志文章
doi:10.1007/s10571-008-9260-7
更新日期:2008-09-01 00:00:00
abstract::Organotellurium compounds have been synthesized since 1840, but pharmacological and toxicological studies about them are still incipient. Therefore, the objective of this study was to verify the effect of acute administration of the organochalcogen 3-butyl-1-phenyl-2-(phenyltelluro)oct-en-1-one on some parameters of o...
journal_title:Cellular and molecular neurobiology
pub_type: 杂志文章
doi:10.1007/s10571-010-9547-3
更新日期:2010-10-01 00:00:00